LUMBAR PUNCTURE:

 CSF can be obtained from lumber puncture from subchondral space. It is the easiest
method and commonly used.
 Lumbar puncture, also called a spinal tap, is a test where a small amount of fluid is
removed from the space around the spinal cord. The fluid is tested for problems.
 It is done by passing a needle in the interspace between the third and fourth lumbar
spine.
 A lumbar puncture can help diagnose serious infections, such as meningitis; other
disorders of the central nervous system, such as Guillain-Barre syndrome and multiple
sclerosis; or cancers of the brain or spinal cord.
 Sometimes a lumbar puncture is used to inject anesthetic medications or
chemotherapy drugs into the cerebrospinal fluid.
CSF FORMATION:
 The bulk of CSF is formed by choroid plexuses of the lateral ventricles and lesser
amounts by the choroid plexuses of the third and fourth ventricle.
 Possibly, it is also formed by the capillaries on the surface of the brain and spinal
cord.
 The total quantity of CSF is about 150ml. it is formed at the rate of about 200ml per
hour or 5000ml per day. The normal pressure of CSF is 60 to 100mm of water.
CSF CIRCULATION:
Forms in lateral ventricle
Interventricular foramen
III ventricle
Aqueduct of midbrain
IV ventricle

Central canal of spinal cord 3 apertures in roof- one median and 2 laterals
Open in

Subarachnoid space around spinal

cord and cauda equina Cerebello-medullary cistern
and pontine cistern,

tentorial notch

inferior space of cerebrum

superolateral surface of cerebrum

arachnoid granulations
superior sagittal sinus