1/25/2021 2: Mass Spectrometry - Chemistry LibreTexts
2: Mass Spectrometry
Learning Objectives
This chapter will define construction and use of multiple types of mass spectrometers including GCMS and LCMS systems.
Interpretation of the resulting data will be an important focus.
describe the design of differing types of mass spectrometers
explain how gas chromatography and liquid chromatography are used in tandem with mass spectrometers
explain common ionization methods
describe the function of common mass analyzers
interpret mass spectra
2.1: Introduction to Mass Spectrometry
Individual molecules often fall apart during the mass
spectrometry experiment. As a result, in addition to
measuring the mass of an entire molecule, we also obtain
the weights of various smaller pieces of the molecule.
That may add some confusion to the data. However,
these fragments provide an idea about what parts make
up the whole molecule.
2.3: How the Mass Spectrometer Works
This page describes how a mass spectrum is produced
using a mass spectrometer.
Electrospray Ionization Mass Spectrometry
Injection Stage
MALDI-TOF
Mass Analyzers (Mass Spectrometry)
2.5: Mass Spectrometry Problems
LEGO Exercise
https://chem.libretexts.org/Courses/University_of_Illinois_Springfield/Introduction_to_Organic_Spectroscopy/2%3A_Mass_Spectrometry
2.2: Mass Spectrometry
Mass spectrometry (MS) is a powerful characterization
technique used for the identification of a wide variety of
chemical compounds. At its simplest, MS is merely a tool
for determining the molecular weight of the chemical
species in a sample. However, with the high resolution
obtainable from modern machines, it is possible to
distinguish isomers, isotopes, and even compounds with
nominally identical molecular weights. Libraries of mass
spectra have been compiled which allow rapid
identification
2.4: Interpretation of Mass Spectra
13C
Br and Cl
Fragmentation
Fragmentation Patterns in Mass Spectra
Molecular Ions
Molecular Ion and Nitrogen
Organic Compounds Containing Halogen Atoms
The Molecular Ion (M⁺) Peak
The M 1 Peak
2.6: ESI-QTOF-MS Coupled to HPLC and its
Application for Food Safety
Mass spectrometry (MS) is a detection technique by
measuring mass-to-charge ratio of ionic species. The
procedure consists of different steps. First, a sample is
injected in the instrument and then evaporated. Second,
species in the sample are charged by certain ionized
methods, such as electron ionization (EI), electrospray
ionization (ESI), chemical ionization (CI), matrix-assisted
l d / ( )
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laser desorption/ionization (MALDI).

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