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Ix. CSF Analysis
Ix. CSF Analysis
Ix. CSF Analysis
1
Denielle
Genesis
B.
Camato
IX.
CSF
ANALYSIS
ANALYSIS
O F
URINALYSIS
AND
BODY
FLUIDS
|
REVIEWER
CYTOCENTRIFUGATION
As little as 0.1mL of CSF combined with one drop of
30% albumin produces an adequate cell yield when
processed with the cytocentrifuged.
Addition of albumin increases the cell yield and
TOTAL CELL COUNT decrease the cellular distortion frequently seen on
cytocentrifuged specimens
Clear specimens may be counted undiluted, provided
no overlapping of cells is seen during the microscopic
CSF CELLULAR CONSTIUTENTS
examination
Adults have usually predominance of lymphocytes to
When dilutions are required, calibrated automatic
monocytes (70:30) ratio whereas monocytes are
pipettes are used
more prevalent in children.
Dilutions for total cell counts are made with normal
Pleocytosis- increased numbers of these normal
saline mixed by inversion and loaded in the
cells is considered abnormal, as the finding of
hemocytometer by a Pasteur pipette
immature leukocytes, eosinophils, plasma cells and
Cells are counted in the four corner square on both
macrophages, increased tissue cells & malignant cells
sides of the haemocytometer
High WBC count of which the majority of the cells
Clarity Dilution Amount of Amount of
are neutrophils is indicative of bacterial meningitis
Sample Diluent
High percentage of lymphocytes & monocytes
Slightly hazy 1 : 10 30μL 270 μL suggests: viral, tubercular, fungal, or parasitic origin
Cell forms differing those found in blood include
Hazy 1 : 20 30μL 570 μL macrophages, choroid plexus, and ependymal cells,
Slightly 1 : 100 30μL 2970 μL spindle-shaped cells, and malignant cells
cloudy
Slightly 1 : 200 30μL 5970 μL
bloody
Cloudy 0.1 mL of a 9.9 mL
Bloody 1 : 10,000 1:100 dilution
Turbid
WHITE BLOOD CELL COUNT
2
Denielle
Genesis
B.
Camato
IX.
CSF
ANALYSIS
ANALYSIS
O F
URINALYSIS
AND
BODY
FLUIDS
|
REVIEWER