User Manual

AUTOMATED MICROPLATE READER

 Table of Contents

1. INSTALLATION.......................................................................................................... 3
1.1. UNPACKING.............................................................................................................. 3
1.2. INSTALLATION ENVIRONMENT REQUIREMENTS................................................. 3
1.2.1 Transportation and Storage...................................................................................... 3
1.2.2. Operation Environment........................................................................................... 3
1.3. CONNECTION.......................................................................................................... 3

2. FUNCTION.................................................................................................................... 4
2.1. INTRODUCTION......................................................................................................... 4
2.2. FRONT VIEW (FIGURE 1)......................................................................................... 4
2.3. REAR VIEW (FIGURE 2)............................................................................................ 5

3. OPERATION................................................................................................................................ 6
3.1. POWER-ON................................................................................................................................. 6
3.2. MAIN MENU............................................................................................................................... 6
3.3. MEASURE................................................................................................................................... 6
3.4. PROGRAM.................................................................................................................................. 8
3.4.1. Edit Test................................................................................................................................... 9
3.4.2. New Test................................................................................................................................. 13
3.4.3. Delete Test.............................................................................................................................. 14

3.5. QUALITY
CONTROL…...................................................................................................................................... 14

3.6. SETUP........................................................................................................................................ 16
3.6.1. Date and Time.......................................................................................................................... 16
3.6.2. Plate Mixing.............................................................................................................................. 16
3.6.3. Light Source.............................................................................................................................. 17
3.6.4. Printer...................................................................................................................................... 17
3.6.5. Filter........................................................................................................................................ 17

3.7. SERVICE................................................................................................................................... 18
3.7.1. Data Transfer.......................................................................................................................... 18
3.7.2. Result Review........................................................................................................................ 18
3.7.3. Standard Review..................................................................................................................... 18

3.8. RAPID MEASURE..................................................................................................................... 19

4. MAINTENANCE............................................................................................................................. 20
4.1. NOTICE....................................................................................................................................... 20
4.2. MAINTENANCE........................................................................................................................... 20
5. TECHNICAL SPECIFICATIONS.................................................................................................... 21

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6. TROUBLESHOOTING................................................................................................................... 22
7. WARNING.............................. ........................................................................................................ 23

1. Installation

1.1. Unpacking

 When you receive your Erba Lisascan II Microplate Reader, carefully unpack, and
check the system. If you see any damages, promptly inform the company.

 Put away the packaging material for future use.

 Check your Erba Lisascan II Microplate Reader accessories against the packing list.
If you find anything missing, promptly inform the company.

1.2. Installation Environment Requirements

1.2.1 Transportation and Storage

 Ambient temperature (-20 - 55)℃

 Relative humidity ≤93%

 Atmospheric pressure (50 - 106) kPa

1.2.2. Operation Environment

 The Erba Lisascan II Microplate Reader should be free of dust, vibration, exposure to
corrosive and flammable gases, direct interference by strong current and magnetic
field, as well as direct sunlight. Moreover, enough space should be kept behind the
system for good ventilation.

 Ambient temperature: 10 - 40℃.

Relative humidity: ≤85%
Atmospheric pressure: 86 - 106kPa

 Power requirements: AC 220/110V, 50/60Hz

1.3. Connection

 Please check if the voltage conforms to requirements of the system. Make sure the
power socket is grounded correctly; if not, you must connect a single ground wire to
the Grounding port on the device.

 Please connect the printer to the Printer port on the device through a data cable.


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  Insert one end of the power cord into the Power socket on the rear panel of the device
and other end to the power supply.

2. Function

2.1. Introduction

 The Erba Lisascan II Microplate Reader is an 8-channel optical photometric system,

which can be used to measure the optical density of the vertical light. It is a
specialized analyzer to hold microplates, and then take readings and process data.

 The Erba Lisascan II Microplate Reader adopts the touch screen, which is a human
interface and comfortable to operate.

 With the advanced high-speed microprocessor and advanced software, the Erba
Lisascan II Microplate Reader makes operating of the system, simpler and more
convenient, calculation of results quicker, and thereby increasing operator’s efficiency.

2.2. Top View (Figure 1)

Touch Screen Sample Compartment

Figure 1
2.3. Rear View (Figure 2)

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 Printer Port Serial RS232 Port Power Socket Power Switch Grounding

Figure 2

3. Operation

3.1. Power-on

When you Put the Power switch ON (|), the system will begin self-check and then display the
Main Menu screen (Figure 3). After powering on the system, it takes 10 minutes for warm-up
of the lamp.

Main Menu Jan.18, 2008

18: 18: 18

Measure Absorbance

Program Set Up

Quality Control Service

Figure 3

3.2. Main Menu

The Main Menu displays the function keys and current date and time. You can enter the
submenu screen by touching corresponding function key.

3.3. Measure


 Touch the Measure button, the Select Test screen as shown in Figure 4 .
 Touch Pgdn to display the tests of other pages circularly (42 tests in total, 3 pages).
Touch the Exit button to return to the Main Menu.

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 Figure 4 Select Test screen

After you select the test, the Plate No. and Vibrancy screen (Figure 5) is displayed.

Plate Mix

Figure 5 Plate No. and Vibrancy screen

Touch Plate No. and enter the Plate No. in the Plate No. screen (Figure 6). As shown in
figure 6, the CLR button is to delete what you have entered. After entering, touch to
confirm and return to the Plate No. and Vibrancy screen. If you touch Exit, then the Plate No.
is 00. Touch OK to begin measuring and analyzing the plate. If you need to mix the sample by
vibrating the plate before measuring, touch Plate Mix and then the OK button.

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 Figure 6 Plate No. screen

3.4. Program The Program screen includes Edit Test, New Test and Delete Test (Figure 7).
Touch Exit to return to the Main Menu.

Figure 7 Program screen

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3.4.1. Edit Test

Touch Edit Test to select the test that you want to edit (Figure 4), then enter the
Edit test screen (Figure 8).

Note: After setting, touch OK to save your settings.

Figure 8 Edit Test screen

Touch the Prim. Wav. button, a menu will then appear to ask you to select the wavelength.
Please select as instructed by the package insert.

NOTE: Don’t close all the filters; otherwise the alarm message “Lamp L” will appear.

Touch the Sec. Wav. Button, a menu will then appear to ask you to select the wavelength.
Please select as instructed by the package insert.
In the Plate Layout screen (Figure 9-1), the right column is for selecting the well position you
want to set, use the and buttons to select different well columns. The
box on top right corner displays the current strip being set. Touch Exit to return to the screen
as shown in figure 8.
9

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 Figure 9-1 Plate Layout screen

After selecting the well position, the screen as shown in figure 9-2 will be displayed.
The left column is for setting the filling type and filling No. of the selected well position
(Sample: 1-96; Standard: 1-10; Quality Control: 1-10; Neg./Pos. control: 1-12; Blank well: 1-
12), use the Pgdn button to view each No. of filling type. Touch the selected well position
once more to exit its filling, refer to A01 in figure 9-2.

Figure 9-2 Plate Layout Menu

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 You can set the sample position quickly by using the Automatic button as shown in
figure 9-1. Since this function sets No. only for the original sample wells or blank wells,
you should touch Delete All (Figure 10) to delete all settings of the well positions before
auto filling. Then enter the Blank, Standard and Quality Control, etc, touch Automatic
and select Hor. Strip or Ver. Strip and No. of wells that you want to repeat.

Figure 10 Automatic screen

Touch the Calculation Mode button as shown in figure 8, and then select a method in the
right list appeared.

1) Absorbance: Read the absorbance of the sample directly, no submenu is displayed.

2) Qual Calculation: You should set Qual Grouping, Cut-off, Neg. Range, Pos. Range,
Validity Confirm and so forth. Please make settings as instructed by the package insert.
a) Qual Grouping 1-4: You can divide the qualitative results into four groups (Negative-,
Positive+, Weak Positive-+ and Strong Positive ++) in accordance with the cut-off you have
set.
b) Cut-off 1-3: Enter values of a, b and c in the Cut-Off formula a*NC+b*PC+c.
Where, NC - Absorbance of the negative control well
PC - Absorbance of the positive control well

c) Neg. Range: When the absorbance exceeds the required range, the device will calculate
automatically with the negative control limit you have set.
d) Pos. Range: When the absorbance exceeds the required range, the device will calculate
automatically with the positive control limit you have set.
e) Validity Confirm 1-3: Set the range of absorbance for blank wells, positive and negative
control wells. The device will not calculate results when the absorbance exceeds the range.

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 3) Quant Calculation: Some parameters are added on the basis of qualitative settings,
such as STD Concentration, Unit, Reference, Calibration Rule and Factor. Please make
settings as instructed by the package insert.
a) STD Concentration: You can set the concentrations of up to 8 standards. When
setting, you should begin with 1 from low concentration to high concentration. If No.
of standards is less than 8, you must set the remaining concentrations to 0.
b) Unit: Enter the concentration unit of the selected test.
c) Reference H: Enter the upper limit for the selected test.
d) Reference L: Enter the lower limit for the selected test.
e) Calibration Rule (C - Concentration, A - Absorbance, k - Slope, b - Intercept)

Factor
Formula: A =kC +b.
k and b should be entered by the operator manually.

One Point Linear

Formula: A =kC,
This calibration rule requires only one standard to obtain the parameter k.

Two Point Linear

Formula: A =kC+b, ,
This calibration rule requires two standards. C and C are concentrations of
1 2
standard 1 and 2; A and A are their absorbance.
1 2
Multipoint Linear
Formula: A =kC+b.
You can get values of k and b through the method of least squares.
This calibration rule requires at least 3 standards.

Nonlinear
Draw a broken line passing through the standard concentration and its absorbance.
Measure the absorbance of the waiting samples and check relevant concentration
on the broken line. This calibration rule requires at least 3 standards.

Multipoint Percent
The maximum absorbance of the standards is 100%. This calibration rule requires at least 3
standards as the multi-point broken line.

Exponent
Formula: A = k lnC + b
This calibration rule requires at least 3 standards.

Logarithm
bC
Formula: A= ke
This calibration rule requires at least 3 standards.
b
Power function A= kC
This calibration rule requires at least 3 standards.

Factor Setup: Enter the calibration parameters K and b which are which are mainly used
in the factor calculation method.

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3.4.2. New Test

Touch the New Test button to select the test that you want to add or modify (Refer to Fig.4
for Select Test screen), and then enter the New Test screen (Figure 11).

Figure 11 New Test screen

In the New Test screen, if you touch a button repeatedly, the screen will display the next number,
letter or symbol in circular way. NEXT is used to move the cursor rightward to enter the next
character. If you enter a wrong character, delete it with the CLR button and enter again. Touch YES
to save what you have entered and Exit to return to the Program screen (Figure 7) without saving your
entering.

3.4.3. Delete Test

Touch the Delete Test button; select the test that you want to delete (Figure 4).

3.5. Quality Control

Touch the Quality Control button, select the test that you want to view (Figure 4), then the
QC plot of this test will be displayed (Figure 12.). Touch the Settings button to set the QC
parameters of this test (V - Mean, SD - Standard Difference). If you want to print the QC data
and QC plot, touch the Print button.

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 Figure 12 QC Plot screen

After touching the Delete Data button, you need to select Del. Last Month or Del. All Data,
as shown in figure 13. Touch Exit to return to the screen as shown in figure 12.

Figure 13 Delete Data screen

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3.6. Setup
In the Setup screen (Figure 14), you can set the date and time, vibrant speed, light source, printer and
filter.

Figure 14 Setup screen

3.6.1. Date and Time

Touch the Date and Time button to set Year, Month, Day, Hour and Minute one by one.
Touch YES to confirm your settings and proceed to the next option until you finish all settings.

3.6.2. Plate Mix

The Vibrancy screen (Figure 15) includes Vibrant Speed and Vibrant Time. There are three speeds
available as High, Normal and Low. The vibrant time should not be longer than 60s.

Figure 15 Vibrancy screen

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3.6.3. Light Source

This button is used to turn on/off the lamp temporarily for the sake of the lamp’s lifespan.

NOTE: The lamp should be turned on and preheats for 10 min before testing;
otherwise the alarm message “Lamp L” will appear.

3.6.4. Printer

This button is used to enable / disable the printer, so you can select whether to print the
results immediately after testing.

3.6.5. Filter

The Lisascan II Microplate Reader leads the air blank absorbance of each filter in the 4
channels (F5 is spare), which is used to check the photometric system. The default
absorbance of the 405 - 630 filters is less than 0.500. However, it may increase gradually in
the result of dust, humidification and moldiness when the device is used for a long time. If the
absorbance is greater than 1.000, the operator should clean the photometric system or
replace the accessories like the filters.

3.7. Service

There are three options in the Service menu:

Data Transfer
Result Review
Standard Review

3.7.1. Data Transfer

Touch the Data Transfer button to transport the test data to the computer which is
connected to the Lisascan II Microplate Reader through the serial RS232 port.

3.7.2. Result Review

Touch the Result Review button and enter the No. of Plate that you want to search, and
then touch YES to display the results. Touch the button to display circularly the
results of column 0-6 and 7-12.

3.7.3. Standard Review

After touching the Standard Review button and selecting the test that you want to search,
the screen will display the standard absorbance and relevant concentration, as well as the
calibration curve, as shown in figure 16. However, if the selected test doesn’t use the Quant
Calculation Mode, the results will not be displayed).

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 Figure 16 Standard Review screen

3.8. Rapid Measure

This function is used to read the absorbance of the waiting samples quickly. After touching the Rapid
Measure button, select the primary and secondary wavelength and enter the No. of samples. Touch
OK to start testing (Figure 17).

Plate Mix

Figure 17 Rapid Measure screen

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 4. Maintenance

4.1. Notice

To ensure reliable performance and excellent operation of the Lisascan II Microplate Reader,
the operator must operate and maintain the device strictly as instructed by this Operation
Manual.

4.2. Maintenance

The Lisascan II Microplate Reader is a precision optical measuring system. Therefore, it’s
necessary to perform the daily maintenance, which includes:
1) Keeping the operating environment dry and clean. Covering the sample compartment after
using to prevent from dust.
2) Keeping the mechanical and electrical parts of the device away from water and reagents.
3) Switching off the power supply while cleaning the surface and screen of the device. Be sure
to use soft cloth with mild detergent.

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 5. Technical Specifications

User Interface : Large Touch Screen LCD with Back Light

Nos. of Programs : 42
Plate Type : 96 well plate ( V, U and Flat Bottom)
Wavelength : Accuracy (±2nm), Half width (≤12nm), Peak transmittance (≥35%).
Wavelength range : 400 – 800 nm
Filters : 405 nm, 450 nm, 492 nm, 630nm. 4 free positions for optional filters
Reading range : 0 - 3.5A
Reading Unit : 8 Channel
Light Source : Tungsten – Halogen 12V/20V
Absorbance resolution : 0.001A
Absorbance accuracy :: ±0.03A (0 - 2.5A)
Repeatability (CV, %) : ≤1%
Linearity : <-2.5%
Stability : ≤ 0.005A/10min
Reading Speed : Single wavelength <5s/plate (96-well plate);
Dual wavelength <10s/plate (96-well plate).
Plate Mixing : Speed (High, Normal, Low);
Plate Mix Time : (0 - 60s)

Operating Environment
Power supply : AC220V/110V, 50/60Hz
Input Power : 100VA
Ambient Temperature : 10-40oC
Storage Temperature : 10-50oC
Relative Humidity : Upto 90% relative humidity without condensation
Weight : Approx. 9.6 kgs

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 6. Troubleshooting

Symptoms Causes Actions to Take

Can’t start up The power cord is not Re-connect the power cord properly.
connected correctly.
Replace the fuse
The fuse is broken

No display, no backlight The LCD display or inverter Contact our local distributor or local
defective agency

The sample compartment The optical coupler is with dust Remove the dust and replace the
works abnormally or defective optical coupler

Sound is abnormal during The sample compartment is Reposition the plate

operation blocked
Remove the foreign matter
Foreign matter exists
Contact our local distributor or local
Mechanical movement is agency
abnormal

Can’t print The printer is not connected Check the connection between the
properly printer and the device

Readings are abnormal The lamp is aging or defective Replace the lamp

The filter is aging or defective Replace the filter

The electric circuit defective

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 7. Warning

The Lisascan II Microplate Reader is a compact in vitro diagnosis instrument. It may bring risks to the
patients indirectly, and accompanying risks to the operator and environment. To avoid these risks, be sure to
read the following instructions carefully.

This device adopts AC 220V/110V which may bring electrical harm to human body. Don’t open the rear cover
before switching off the power supply. If you need to open the rear cover to maintain the device, you’d better
contact our service personnel or professionals.

For the patient samples are bio hazard and will cause infection, be sure to dispose of the waste in accordance
with relevant regulations of your hospital.

NOTE: Don’t operate the system unless you have read this Operation Manual carefully.

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