Espiritu, Condeliza Jewel S. Ms.

Rayala
12- Crisologo 01/24//2021

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Activity 1.1 Understanding Enzyme Activity

Enzymes are like other chemical catalysts. They partake in the reaction
without getting affected. In other words, they speed up the chemical reactions
inside the cells without getting consumed. Enzymes are affected by the hydrogen
ion concentration (pH) and the temperature. Enzymes are highly specific compared
to other catalysts, and each enzyme is specialized for one reactant substance. This
reactant substance is called substrate, and it is specialized for one type of reaction
or a few reactions. Enzymes lower the activation energy required to get the reaction
started. Collectively, these are the most important properties of the enzyme.
This worksheet covers how temperature and pH affect the rate of enzyme
catalyzed reactions. Write your answers in a blank paper.
1. Consider the graph below:
Label the optimum temperature for the enzyme as ‘A’
Label the point at which the enzyme has been completely denatured as ‘B’

2. Explain why the rate of reaction initially increases with temperature

3. The graph below shows rate of reaction data for 2 different enzymes. One of
these enzymes are found in the stomach, the other is found in the mouth.
 a) Which of these lines is more likely to indicate the enzyme found in the
stomach? Explain your answer.
b) Both these enzymes have the same optimum pH, TRUE or FALSE?
4. Explain, in terms of bonding, why the rate of reaction gradually falls once the
pH increases above the optimum rather than denaturing straight away
5. An enzyme-controlled reaction was carries out at 36°C. After 3 minutes, 240 cm 3
of product had been produced. Calculate the rate of reaction is cm 3/s.
Rate of reaction = …………… cm3/s

Answers:

1.
2. The rate of reaction is initially increasing with the rise of temperature because it
has ideal kinetic energy. Therefore, there is an increased chance of collision
between the substrate and the enzyme’s active site.

3.
a) The solid black line is more likely to be the enzyme found in the stomach
because our stomach is very acidic and has a low pH of 2.
b) FALSE
4. The rate of reaction gradually falls instead of denaturing right away because
when the bonds surrounding the active site break, the active site also changes
shape. Then, the substrate can no longer fit, which leads to the forming of fewer
enzyme-substrate complexes and less product.

5. Rate of reaction = 240 cm3 ÷ 180 s

= 1.33 cm3/s