MINIPORYL™

PICTURE-PERFECT PORE MINIMIZER

 PICTURE-PERFECT PORE MINIMIZER
3-IN-1 PORE SHRINKING ACTION
•Improves keratinocyte differentiation:
PRIMER-LIKE HIGH DEFINITION RESULT ↑involucrin, ↑filaggrin, ↑keratin10
•Biological pore refining effect •Reduces excess sebum production in sebocytes:
•Long term action complementary to a ↓5α-reductase type I
instant pore filling primer •Improves skin contraction

NATURAL ORIGIN
Red clover flower M INIPORYL™ CUSTOMER BENEFITS
extract titrated in
MINIPORYL™ • Reduces the appearance of
enlarged pores
Biochanin A
• Refines skin surface
• Fast action in only 15 days!
• Clinically proven on women,
men and young adults
PERFECT SKIN IDEAL = PORELESS EVEN SKIN TEXTURE
Photoshopped ideal Reality!
PORE-FILLING PRIMERS: AN IMMEDIATE SOLUTION
TO ERASE PORES
• Silicone-based primers fill in pores to instantly smooth skin surface
• Initially used for actresses and models to perfect their image
• Physical action => short term effect
Instant pore minimizing effect of a pore-filling primer

Need for a complementary biological solution

to have a long-term treating effect!
 WHY DOES A PORE ENLARGE?
• Pore is the opening of hair follicule, and allows the elimination of sebum
and sweat.
• 3 causes are responsible for its enlargement:

1
INCOMPLETE KERATINOCYTE DIFFERENTIATON
 Corneocytes retain nucleus (parakeratosis)
 Abnormal desquamation
 Accumulation of cell debris clogs pores

3
2 LOSS OF SKIN TONICITY AND FIRMNESS
 Skin relaxation
HYPER-SEBORRHEA  Pore wall collapse
 Higher sebum flow
 Adaptation of the pore size
to outpour sebum excess

Worsen by sun, tabacco, food, bad cleansing,…

MINIPORYL™
• Red clover flower extract titrated in biochanin A (≥0.4%)

• Traditional uses of red clover: purification and rebalancing properties

• 3-in-1 action on pore enlargement causes

1
Keratinocyte differentiation improvement

3
Astringent action

2
Decrease in
sebum production
 IN VITRO STUDIES
1 Improvement of keratinocyte differentiation
2 Decrease in sebum production:
• Inhibition of 5α-reductase type I activity
• Inhibition of lipid synthesis in sebocytes
3 Increase in skin astringency
 IMPROVEMENT OF KERATINOCYTE DIFFERENTIATION 1
DESQUAMATION
In vitro test protocol SKIN BARRIER
(corneocyte) Filaggrin
• Keratinocyte culture (HaCaT) in contact with 0.1% Miniporyl™ during 4 days.
LATE DIFFERENTIATION Keratin 10
• Quantification of immunolabelled differentiation markers by image analysis Involucrin
(fluorescent green): Involucrin, Filaggrin and Keratin 10. EARLY DIFFERENTIATION Keratin 10

PROLIFERATION
(basal kertinocyte)

Markers
(green)

Cell nucleus
(blue)

Miniporyl™ improves keratinocyte differentiation

at any stage to obtain nucleus free corneocytes
 INHIBITION OF 5α-REDUCTASE TYPE I ACTIVITY 2
In vitro test protocol
• Normal human dermis fibroblast culture in contact with 0.2%, 0.5% and 1% Miniporyl™
• Quantification of DHT (dihydrosterone) and testosterone by RP-UPLC-ESI-MS
• Calculation of 5α-reductase inhibition activity

Testosterone

5-a reductase

DHT

Miniporyl™ inhibits the 5a-reductase type I activity with a

dose-dependent effect leading to a decrease in sebum production
INHIBITION OF LIPID PRODUCTION IN SEBOCYTES 2
In vitro test protocol
• Primary human sebocyte culture in contact with 0.0025%, 0.05% and 0.1% Miniporyl™
• Quantification of lipid synthesis (96% of sebum composition) by fluorescence

Fluorescent lipids
in sebocytes

sebocytes

Miniporyl™ inhibits sebum production to reduce oily flow

INCREASE IN SKIN ASTRINGENCY 3
In vitro test protocol
• Culture of collagen lattices + normal human dermis fibroblasts (NHDF) in contact with 0.2% Miniporyl™
• Evaluation of the contraction of collagen lattices after 24h by mesuring their diameter

Miniporyl™ increases skin contraction properties to tight pores up

 MINIPORYL™ - IN VITRO RESULTS SUMMARY

1
Keratinocyte differentiation improvement
↑fillaggrin, ↑involucrin, ↑keratin10

3
Astringent action
contraction of collagen lattice

2
Decrease in sebum production
-inhibits 5α-reductase
-decreases lipids production in sebocytes
CLINICAL STUDIES
• Seboregulation action
• Reduction in pore size and number
TESTED FORMULA
Active formula Placebo
Ingredient INCI name
% %
Deionized Water Water 85.65 87.65
Dissolvine™ Na Tetrasodium EDTA 0.10 0.10
Chlorphenesin Chlorphenesin 0.30 0.30
Phenexyethanol Phenoxyethanol 0.80 0.80
Sodium Acrylates Copolymer (and)
Lecigel™ 1.75 1.75
Lecithin
LIPEX® 102 Butyrospermum Parkii (Shea) Butter 1.00 1.00
Cetiol® CC Dicaprylyl Carbonate 3.00 3.00
Sweet Almond Prunus Amygdalus Dulcis (Sweet
5.00 5.00
Oil Almond) Oil
Vitapherole® Tocopherol (and) Helianthus annuus
0.20 0.20
E1000 (Sunflower) Seed Oil
SG 38241 Fragrance 0.20 0.20
Isopropanediol (and) Trifolium
Miniporyl™ 2.00 0
Pratense (clover) flower extract
REGULATION OF SEBUM PRODUCTION
In vivo test protocol
• 2 groups of 20 men & women with oily or combination skin (18 to 55 years)
• 2x/D application of a cream with 2% Miniporyl™ for a group vs. a placebo for another group during 30 days
• Sebum measurement at D0, D15 and D30 with Sebumeter® and Sebufix® + Visioscan®

Up to -38%! Up to -35%! Up to -42%!

Sebufix® picture
Oily spots
Vol. 12 (28 years old)
D0 D15 D30

Miniporyl™ reduces the sebum production to reduce oily flow.

REDUCTION IN PORE SIZE AND NUMBER
In vivo test protocol
• 2 groups of 20 men & women with oily or combination skin (18 to 55 years)
• 2x/D application of a cream with 2% Miniporyl™ for a group vs. a placebo for another group during 30 days
• Quantification of conspicuous pores (>0.25mm) on cheeks and nose by image analysis (specific filter)
• Self-assessment

Up to -80 %!

Miniporyl™ reduces the size and the appearance of pores

for a flawless skin
REDUCTION IN PORE SIZE AND NUMBER
BEFORE (D0) AFTER (D15) AFTER (D30)
-48% -45%
Vol 11 - Young adult
23 years old

1946 pores > 0.25mm

-1% -39%

Vol 2 - Woman
55 years old

2030 pores > 0.25mm

-5%
-80%

Vol 8 - Man
50 years old

1294 pores > 0.25mm

SELF-ASSESSMENT
After 30 days of application

Miniporyl™ refined and smoothes skin surface

PRODUCT INFORMATION

INCI NAME Isopentyldiol (and) Trifolium Pratense (Clover) Flower Extract

ADDITIVE None
APPEARANCE Colourless to yellowish transparent liquid
FORMULATION Incorporation at the end of the formulation at a temperature below 40oC
DOSAGE 0.5-1%: preventive face care
1-2%: intensive face care
OPTIMUM PH 4-8
APPLICATIONS • Combination and oily skin care • Anti-pollution care • Men care
• Acne-prone skin care • Sun face care • Primer
• Uneven skin care • Cleansing products • Make up
• Urban care • Teenager care • Spa care

CHINA COMPLIANT PRESERVATIVE FREE

TOXICOLOGICAL STUDIES
• Skin Irritation (single patch test) (tested concentration: 10%)
• Eye Irritation (BCOP) (tested concentration: pure)
• Sensitization (tested concentration: 10%)
• Genotoxicity (AMES) (tested concentration: pure)
• Phototoxicity (3T3 NRU) (Tested concentration: 0.2%)

ECOTOXICITY
• Biodegradability
• Aquatoxicity (daphnies)

Excellent safety profile

THANK YOU!