TWO DIMENTIONAL PAPER CHROMATOGRAPHY

Introduction
Two dimensional technique is another complex set up which is used to separate complex
mixtures. In this method the development of the chromatogram is done as in the previous
methods.
Solvent is placed at the bottom of the tank and the filter paper saturated with the stationary phase
is then kept inside the tank. The development occurs up wards. But very slowly because it is
against the gravity as well as the compound is a complex one.
After few hours the filter paper is turned 900 clockwise and the tank is filled with a different type
of solvent. If there is no pronounced separation, then development is proceeded to the “c” stage.
Again the filter paper is turn 900 clockwise and used another solvent. This will probably end up
with a satisfactory separation. If not again it should be turned and use another solvent.
Although this will take some time, this allows a high degree of separation.

Figure 1