HIMALAYAN EDIBLES PRIVATE LIMITED PAKISTAN

Salt Packaging Unit Manzoor Park Saggian Road Lahore

www.himalayanedibles.com

IN HOUSE LABORATORY TEST RECORD

IODOMETRIC LAB TEST PROFORMA (TABLE SALT)

(Method: Pakistan Standards 1669-2008)

Date of Performing Lab test: 11th. Nov. 2020

A. PURPOSE OF THE Determination of Iodine contents in Food Grade

CHEMICAL TEST: Iodized Himalayan salt.

B. DETAILS OF SUPPLY:

1 Substance: Iodized Table Salt

2 Item Code

3 Name of Supplier:

4 Source of Raw Material

5 Supplier's Item Code

6 Purchase order

7 Nature of Packaging:

8 Date of Supply:

9 Qty. of Batch:

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 HIMALAYAN EDIBLES PRIVATE LIMITED PAKISTAN
Salt Packaging Unit Manzoor Park Saggian Road Lahore
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C. SAMPLING:

1 Method of sampling: (i) Randomly Selected Bags ___________________

(ii) Weight of Sample from Each Bag. __________

(iii) Numbers of Sample. ______________________

(iv) Mixed all the three samples thoroughly in

container and put in Large Glass Cylinder.

(v) Covered the sample using glass lid for further use.

PRINCIPLE:

The Iodine content in iodized salt containing potassium iodate is estimated by a

process called iodometric titration. Free iodine reacts with sodium thiosulphate solution as
follows:

2 Na2S2O3 + I2 → 2NaI + Na2S4O6

Sodium Iodine Sodium Sodium
thiosulphate iodide tetrathionate

Sulphuric acid to solution of iodized salt which liberates iodine, which is titrated with
sodium thiosulphate. Starch is used as an external indicator. Potassium iodide solution is
added to keep the Iodine in the dissolved state.

D. APPARATUS:

1 Analytical Balance / Sc Scientific weighing scale digital. 1 Set

least count 1.0 mg with Calibration Weight.
2 60mm Glass Petri Dish with Cover (Pyrex) 2 Nos.
3 Lab Spatula 6" 2 Nos.
4 Cylinder 50 ml. (Pyrex) 1 No.
5 Beaker 50 ml. (Pyrex) 1 No.
6 Beaker 100 ml. (Pyrex) 1 No.
7 A magnetic stirrer or glass stirrer 1 No.
8 100 ml conical flask with stopper (Pyrex) 1 No.

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 HIMALAYAN EDIBLES PRIVATE LIMITED PAKISTAN
Salt Packaging Unit Manzoor Park Saggian Road Lahore
www.himalayanedibles.com

9 100 ml Volumetric flask (Pyrex) 1 No.

10 200 ml Volumetric flask (Pyrex) 1 No.
11 1000 ml Volumetric flask (Pyrex) 1 No.
12 10 ml Pipette with cleaning brush. 2 Nos.
13 Reagent Bottle 4 Nos.
14 Class A Glass Burette, 25 ml 1 No.

15 Spirit Lamp or Lab hot Plate 1 No.

16 Lab funnel 100 mm dia. 1 No.

17 Lab Filter Paper (Whatman) 1 box

E. PREPARATION OF REAGENTS:

1/4. 2 N Sulphuric acid:

Chemical Required Empty wt. Gross wt. Net wt. / Remarks

Quantity of Lab. 60 of Lab dish Volume
mm Dish 60 mm + (Actual)
chemical
Double distilled
/ deionized 90 ml -- -- Put in volumetric
water flask 100 ml
Concentrated
H2SO4 0.60 ml -- -- Add Slowly to 90 ml
ACS Grade DD water
swirl flask
Double distilled Added to make
/ deionized 100 ml -- -- 100ml and shifted to
water reagent bottle

Note: (Stored in a cool, dark place.)

Date of Preparation: _________/2020

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 HIMALAYAN EDIBLES PRIVATE LIMITED PAKISTAN
Salt Packaging Unit Manzoor Park Saggian Road Lahore
www.himalayanedibles.com

2/4. 10% potassium iodide Solution:

Chemical Required Empty wt. Gross wt. Net wt. / Remarks

Quantity of Lab. 60 of Lab dish Volume
mm Dish 60 mm + (Actual)
chemical
Potassium
iodide 10 g Put in beaker
(KI, AR) 100 ml

Double distilled
/ deionized 100 ml -- -- Put in beaker
water And dissolve using
stirrer and shifted
to reagent Bottle
Note: (Stored in a cool, dark place.)
Date of Preparation: _________/2020

3/4. Sodium thiosulphate (Na2S2O3) Solution:

Chemical Required Empty wt. Gross wt. Net wt. / Remarks

Quantity of Lab. 60 of Lab dish Volume
mm Dish 60 mm + (Actual)
chemical
Sodium
thiosulphate 1.24 g Put in volumetric
(Na2S2O3) flask 1000 ml
Double distilled
/ deionized 1000 ml -- -- Put in volumetric
water flask 1000 ml and
swirled flask and
shifted to reagent
Bottle
Note: (Stored in a cool, dark place.)
Date of Preparation: _________/2020

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Salt Packaging Unit Manzoor Park Saggian Road Lahore
www.himalayanedibles.com

4/4. 1% Soluble chemical starch Solution:

Chemical Required Empty wt. Gross wt. Net wt. / Remarks

Quantity of Lab. 60 of Lab dish Volume
mm Dish 60 mm + (Actual)
chemical
Double distilled
/ deionized 100 ml -- -- Put in 100 ml
water beaker, and Boiled
Sodium i) Put in boiled DD
chloride (NaCl) 10 g water while stirring,
reagent (AR) added more salt unti no
more dissolved.
ii) Reheat contents.
iii) Cool contents to
room temperature.
iv) decanted the
supernatant in a clean
bottle. as NaCl
Saturated solution
(Solubility of NaCl in
water is 384 mg/ml at
100oC. )

Double distilled 10 ml -- -- Put in 100 ml

/ deionized beaker, and Boiled
water
Soluble
chemical 1g Put in boiling 10 ml
starch boiling DD water
and dissolve using
stirrer
Sodium
chloride (NaCl) 90 ml -- -- Add NaCl Saturated
reagent (AR) Solution to make 100
Saturated ml Starch solution
Solution and shifted to
reagent Bottle
Note: (discard after performing test.)
Date of Preparation: _________/2020

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 HIMALAYAN EDIBLES PRIVATE LIMITED PAKISTAN
Salt Packaging Unit Manzoor Park Saggian Road Lahore
www.himalayanedibles.com

F. LABORATORY TEST PROCEDURE

Chemical Required Empty wt. of Gross wt. Net wt. / Remarks

Quantity Lab. Dish of Lab dish Volume
60 mm 60 mm + (Actual)
chemical
Sodium Put in 50 ml
Chloride 10.0 g Cylinder
Table Salt
Double distilled Take Put in separate 100
/ deionized 110 ml -- -- ml beaker and
water boiled.
(Final Vol=100 ml)
Boiled Double Slowly put in 50 ml
distilled / 50 ml -- -- Cylinder containing
deionized water Salt sample to
make 50 ml
solution. and
shifted to 100 ml
Conical flask with
stopper.
Using Pipette added
2 N Sulphuric 1.0 ml -- -- to contents of Conical
flask
acid
10% i) Using Pipette
potassium 5.0 ml -- -- added to contents of
Conical flask with
iodide stopper.
ii) Placed stopper.
iii) Flask put in dark
for 10 minutes

Time noted:________

Took the flask out

and shaken.
Note: Color of Solution: Turned Yellow
Sodium Put in 25 ml Burette.
thiosulphate -- -- -- -- and adjusted level to
zero.
Solution Titrate Solution in
flask.

Note: Stop Titration when color turned Pale or light yellow

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 HIMALAYAN EDIBLES PRIVATE LIMITED PAKISTAN
Salt Packaging Unit Manzoor Park Saggian Road Lahore
www.himalayanedibles.com

1% starch Added to flask

solution 1.0 to 5.0 -- -- contents, Color of
Solution: Turned
ml deep Purple
Sodium Continued titration
thiosulphate -- -- -- -- Till the solution
became colorless.
Solution Readings noted in
(in Burette) following table:

Na2S2O3) Solution Na2S2O3) Solution Na2S2O3) Solution

Start Point Burette End Point Burette Vol: Consumed

Take Volume consumed (V1) =

Calculate Iodine contents using following formula and also cross check the Iodine
contents against corresponding value of Sodium thiosulphate Solution consumed in
titration from table :

Iodine Contents (PPM) = 10.58 x V1 (Na2S2O3) Solution) + 0.009

Iodine Contents (PPM) = 10.58 x (_________) + 0.009 = ___________

Iodine Contents (PPM) = corresponding value in table ___________

RESULT:

IODINE CONTENTS: _______ PPM (PARTS PER MILLION)

Signatures Signatures
Muhammad Daud Rana Engr. Rana Naveed Ahmad

Dated: ___________/ 2020___

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