1.

2 - Isolations Game Plan

Soil Bacteria Human Bacteria

Bacillus sp. Pseudomonas sp. S. epidermidis Escherichia coli

-- or -- -- or --
M. luteus Enterobacter aerogenes

Lab 1

Isolation: Soil Sample Soil Sample Patient Skin Swab Patient Rectal Swab

Pasteurize Soil Sodium Benzoate

Suspension Broth
(Selection) (Enrichment)

Streak: Nutrient Agar YGC Nutrient Agar &

EMB
¯ ¯ ¯ ¯
Incubate: 30°C/48H 25°C/1 Week 37°C/48H 37°C/24H

Mother Plate: 04°C 04°C

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Lab 2

Colony Matte White (Turbid Broth) Cream with Clearing Metallic Green
Morphology: -- or -- -- or --
Yellow w/o Clearing Pepto-Bismol Pink

Streak: Nutrient Agar Sodium Benzoate Agar YGC Nutrient Agar

¯ ¯ ¯ ¯
Incubate: 30°C/48H 30°C/48H 37°C/48H 37°C/24H

Mother Plate: 04°C 04°C 04°C 04°C

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AY 2020/2021 1.2 - Lab 1: Isolations Game Plan Page 1

 Lab 3

Colony Matte White Clear/White Cream with Clearing Clear/White

Morphology: -- or --
Yellow w/o Clearing

Gram Stain: Gram Positive Gram Negative Gram Positive Gram Negative
Rods Rods Cocci Rods
(Purple) (Pink) (Purple) (Pink)

Streak onto: Nutrient Agar Nutrient Agar YGC Nutrient Agar

¯ ¯ ¯ ¯
Incubate: 30°C/24H 30°C/48H 37°C/48H 37°C/24H

Mother Plate: 04°C 04°C 04°C 04°C

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Lab 4

Colony Matte White Clear/White Cream with Clearing Clear/White

Morphology: -- or --
Yellow w/o Clearing

Gram Stain: Gram Positive Gram Negative Gram Positive Gram Negative
Rods Rods Cocci Rods
(Purple) (Pink) (Purple) (Pink)

Streak onto: Nutrient Agar Nutrient Agar YGC Nutrient Agar

¯ ¯ ¯ ¯
Incubate: 30°C/24H 30°C/48H 37°C/48H 37°C/24H

Mother Plate: 04°C 04°C 04°C 04°C

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AY 2020/2021 1.2 - Lab 1: Isolations Game Plan Page 2

 Lab 5

Colony Matte White Clear/White Cream with Clearing Clear/White

Morphology: -- or --
Yellow w/o Clearing

Gram Stain: Gram Positive Gram Negative Gram Positive Gram Negative
Rods Rods Cocci Rods
(Purple) (Pink) (Purple) (Pink)

Spore Stain: Spores - - -

(Green)

Biochemical Identification Tests

Catalase - - Fizzes (Positive) -

Oxidase - Purple (Positive) - No Change (Negative)

Streak/Inoculate: - F Agar YGC Tryptone Broth

MR/VP Broth
Citrate Slant
¯ ¯ ¯
Incubate: - 30°C/48H 37°C/48H 37°C/48H

Mother Plate: 04°C 04°C 04°C 04°C

__________________________________________________________________________________________________________________________

Lab 6
¯ ¯ ¯
Results: Fluorescent (Positive) Cream with Clearing ++/--
-- or -- -- or --
Yellow w/o Clearing --/++

Mother Plate: 04°C 04°C 04°C 04°C

AY 2020/2021 1.2 - Lab 1: Isolations Game Plan Page 3