RYU MIZUTANI MICROBIOLOGY

BSN 1Y2- 14

I. Identify the parts of the compound microscope. Choose from the word bank below.

Microscope labeling. (n.d.). The Biology Corner. https://www.biologycorner.com/worksheets/microscope_labeling.html

Word Bank:

-Arm - Eye piece - Iris Diaphragm Adjustment

-Base - Fine Focus Knob -Mirror (or light source)
-Coarse Focus Knob -High-power objective -Revolving Nosepiece
-Condenser -Low-power objective -Stage
-Condenser Focus Knob - oil immersion objective
ANSWERS
1. Body tube
2. Revolving nose piece
3. low- power objective
4. Scanning objective/oil immersion objective
5. Stage clips
6. Diaphragm
 7. Mirror (light source)
8. Eye piece
9. Arm
10. Stage
11. Coarse focus knob
12. Fine focus knob
13. Base

II. Give the functions of each part of the microscope listed in the word bank.
1. Body tube – it connects the eye piece to the objective lens.
2. Revolving nose piece – it holds the objective lenses, viewer spins the nosepiece to select
different objective lenses.
3. Low-power objective- it magnifies 10x and is found above the specimen.
4. Scanning objective/oil immersion objective - it magnifies 4x is the shortest objective and is
useful for getting a general overview of a slide
5. High power objective - magnifies 40x, with total magnification 400x if the eyepiece lens is 10x
power, and it is ideal for observing very fine detail, such as nerve cells in the retina or the
striations in skeletal muscle
6. Stage clips- the platform where the slide is paced.
7. Diaphragm – it controls the amount of light that reaches the specimen.
8. Mirror(light source)- it is used to reflect light from an external light source up through the
bottom of the stage
9. Eye piece- also known as the ocular. This is the part used to look through the microscope.
10. Arm- it gives support to the head of the microscope and it also used when carrying the
microscope.
11. Stage- This is the section on which the specimen is placed for viewing.
12. Coarse focus knob- The coarse focus knob is used to bring the specimen into approximate or
near focus.
13. Fine focus knob- This knob is inside the coarse adjustment knob and is used to bring the
specimen into sharp focus under low power and is used for all focusing when using high power
lenses.
14. Base- it supports the microscope and it’s where illuminator located
III. Research online photomicrographs of specimen observed using LPO, HPO
( Give 2 examples for each )
ANSWERS:
(a) Low power objective

(a) Low power photomicrograph of the biopsy specimen which reveals normal epithelium and
foci of plasma cells separated by connective tissue septa (H and E, ×10). (b) High power
photomicrograph of the biopsy specimen which reveals morphologically normal plasma cells
without atypia (H and E, ×40) a b

(B) Photomicrograph (low power) showing odontogenic epithelial islands with peripheral
palisading (H&E X10). B: high power magnification of neoplastic odontogenic epithelial cells
with peripheral palisading (H&E X40). C: Photomicrograph (high power) of an odontogenic
epithelial island with peripheral palisading, reverse polarity and clear cell changes (H&E X40).
D: Photomicrograph (high power) of the intact surface epithelium (H&E X40)
High power objective

(a) (A) High-power photomicrograph of normal appearing kidney in control rats

(hematoxylineosin × 200 original magnification). (B) Extensive necrosis (bottom right) in
rats receiving morphine (hematoxylin-eosin × 200 original magnification). (C) Tubular
epithelial vacuolization and congestion in rats receiving tramadol (hematoxylin-eosin ×
200 original magnification).

(b) High-power photomicrograph (original magnification, Â100; H&E stain) shows infiltrating
nests and cords of large polygonal cells, separated by prominent fibrous septa. (b) High-
power photomicrograph (original magnification, Â400; H&E stain) depicts abundant
granular eosinophilic cytoplasm and small nuclei within the cells, features indicative of
GCT. (c) Immunohistochemistry shows diffuse reactivity for S100 protein. Nuclei are
small and bland.