EDT 5303

TECHNOLOGY AND
INNOVATION IN EDUCATION
( GROUP 12)

TOPIC :
MECHANISM OF
ENZYME ACTION
1 . N U R F AU ZAN A BT ZU LKIF LI (G 1 027668)
2. N O R ‘AID AH BT LU K M AN (G
1 02731 2)
3. WAN S U H AILA BT WAN IS M AIL (G 1 022282 )
 C O N TE N T

LEARNING
1 OUTCOME

2 INTRODUCTION

PRIOR
3 KNOWLEDGE
DEFINITION OF
4 ENZYME
MECHANISM OF
5 ENZYME
ACTION
6 ASSESSMENT
LE AR N IN G O U TC O M E

4.10 :
Explain the
mechanism of
enzyme
action
IN TR O D U C TIO N

Do you realize the

term of COENZYME
on detergent
products?

CLICK HERE !!
 P R IO R K N O WLE D G E

RECALL !!!

Maltose + Water Glucose + Glucose

ENZYME maltase BREAK

DOWN maltose into 2
molecules of glucose
 P rior knowle d ge

Enzyme
s
Cloth

Stains

How the stains have been

removed from the cloth?
 D E F IN ITIO N O F E N ZYM E

Enzymes are biological

catalyst that direct or guide
almost all cellular reactions.
M E C H AN IS M O F E N ZM E
AC TIO N

‘LOCK AND KEY’

HYPOTHESIS
 M E C H AN IS M O F E N ZM E
AC TIO N
Product

Product

Enzyme Enzyme-substrate complex

EXPLANATION EXPLANATION EXPLANATION

Substrate
 M E C H AN IS M O F E N ZM E
AC TIO N

A specific substrate molecule

arrives at the active site of the
enzyme molecule.
 M E C H AN IS M O F E N ZM E
AC TIO N

The substrate molecule binds to

the active site to form an
enzyme-substrate complex
which is very unstable.
 M E C H AN IS M O F E N ZM E
AC TIO N

Product molecules leave the

active site of the enzyme.

The enzyme is free to take

another substrate molecule
into its active site to catalyse
another reaction.
 M E C H AN IS M O F E N ZM E
AC TIO N
Which substrate can bind to active site of enzyme sucrase?

Sucrose can bind to

active site of sucrase
Enzymes
have
specific
sites called
Sucrose
active site
which bind
to specific
substrates
Sucrase Maltose

Maltose CANNOT bind to

active site of sucrase
 as s e s s m e nt

Question 1 Question 2

Question 3 Question 4
 Q u e s tion 1
Fill the blank with the correct answer.
2. A specific ______________ molecule arrives Product
at the active site of the ______________ molecule.
enzyme-
substrate
2. The substrate molecule binds to the active site of the complex
enzyme to form an ______________.
enzyme

3. The enzyme is free to take another substrate molecule

active site
into its ______________ to catalyse another reaction.
substrate

4. ______________ molecules leave the active site of the

enzyme.
 Q u e s tion 2

Which of the following shows the characteristic of enzymes?

A. Enzyme reaction is specific

A. Enzyme reaction is influenced by pH

C. Enzyme reaction is influence by temperature

D. The structure of the enzyme change at the end

of the reaction.
 Q u e s tion 3

Protease Dissolve starch

stains on cloth

Amylase Ripening cheese

Lipase Dissolve protein

stains on cloth
 Q u e s tion 4

Enzyme is used to wash away the blood

stain on a shirt.
Explain how the enzyme acts on the
blood stain at a maximum rate.

(3 MARKS)

ANSWER :
Blood is made up from proteins. Protease in detergent
will break down protein into amino acids. This reaction
occur effectively at 40 ºC which is optimum temperature
for enzyme action.
THANK YOU ….