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Chromatography Investigation PrintOut
Chromatography Investigation PrintOut
Chromatography Investigation PrintOut
Equipment:
Safety Information
• Strips of chromatography paper Handle using forceps or gloves and
wash hands after handling
• Pencil
Glassware – risk of cuts if broken
• Beaker
Apply pressure to wounds and inform
• Water
teacher immediately
• Pipette
• Drawing pin
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Chromatography Investigation
Method:
Step 1: Using the first pen, make a small spot at one end, about 1 cm from the edge. Make sure you
know which pen this chromatogram relates to.
Step 2: With the drawing pin, secure the other end of the strip to the middle of a wooden pencil and
balance across the top of the beaker.
Step 3: Using the pipette, add water to the bottom of the beaker so that the paper is submerged but the
black ink spot remained above the water line. Ensure you do not splash the paper, or allow it to touch
the sides of the beaker.
Step 4: Observe as the water is drawn up the paper. When it has reached the top, carefully lift out the
paper using the pencil and lie flat to dry.
Step 6: Once dry, compare your chromatograms to the unknown and identify the pen used.
Questions
1. What is a mixture?
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Chromatography Investigation
Answers
1. What is a mixture?
A mixture is made up of two or more different compounds, not chemically joined.
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