HWA CHONG INSTITUTION / 2020-2021 BIOLOGY / Core Idea 2 / DNA Structure & Replication / TBL 1.

2 / PrQ 4

HWA CHONG INSTITUTION

2020-2021 H2 BIOLOGY
CORE IDEA 2: GENETICS AND INHERITANCE (DNA STRUCTURE & REPLICATION)
TEAM BASED LEARNING (TBL) SESSION 1.2

STRUCTURED QUESTIONS

STQ 1: N05 P2 Q3
Fig. 3.1 is a diagram showing DNA replication.

Fig. 3.2

(d) The DNA molecule is described as having anti-parallel strands. Describe the evidence
visible in Fig. 3.1 and Fig. 3.2 that DNA has anti-parallel strands.
[2]

(answer here)

[Total: 8]

Fig. 3.1

(a) Name two molecules required for DNA replication that are not shown in Fig. 3.1. [2]

(answer here)

(b) Explain how Fig. 3.1 shows semi-conservative replication. [2]

(answer here)

(c) Describe how the two original strands determine the structure of the two newly synthesised
strands.
[2]
(answer here)
HWA CHONG INSTITUTION / 2020-2021 BIOLOGY / Core Idea 2 / DNA Structure & Replication / TBL 1.2 / PrQ 4

HWA CHONG INSTITUTION / 2020-2021 BIOLOGY / Core Idea 2 / DNA Structure & Replication / TBL 1.2 / PrQ 4

STQ 2: N13 H2 P2 Q3
Fig. 3.1 shows the mechanism of DNA replication originally proposed by Watson and Crick.
generation 2 generation 1 generation 0
(b) Describe how these results provided evidence for Watson and Crick's proposed mechanism.
Fig. 3.1 [3]
(answer here)
(a) State the name given to this mechanism of DNA replication.
[1] (c) (KIV Central Dogma) List three ways in which transcription is different from DNA replication.
(answer here) [3]
(answer here)
Meselson and Stahl tested Watson and Crick's proposed mechanism. They incubated a culture of
Escherichia coli in a growth medium in which nitrogen was only available as 15N. After several
hours the bacteria were transferred to a growth medium in which the only nitrogen available was (d) (KIV Central Dogma) Explain how the information to synthesise polypeptides is coded for by
14 DNA. [3]
N. The bacteria divided every 20 minutes. DNA was extracted from a sample of the bacteria
every 20 minutes and analysed by ultracentrifuging in caesium chloride solution. The results of (answer here)
these density gradient experiments are shown in Fig. 3.2.
The percentage of the total DNA present in each band is indicated next to each tube. [Total: 10]

Fig. 3.1
HWA CHONG INSTITUTION / 2020-2021 BIOLOGY / Core Idea 2 / DNA Structure & Replication / TBL 1.2 / PrQ 4