Specification 1.07228.5000 Buffered Peptone Water acc. ISO 6579, ISO 19250, ISO 21528, ISO 22964, ISO 6887, FDA-BAM and EP GranuCult ® Specification ‘Appearance (cleamess) lear ‘Appearance (color) yellowish pHvalue (25 °C) 68-72 ‘Growth promotion tes in accordance withthe current version of DIN EN ISO 11133 and the harmonised method of EP, USP and JP. ‘Test as pre-enrichment Specification Inoculum on reference medium (Escherichia = 100 coli ATCC 25922 (WDM 00013)) Inoculum on reference medium (Escherichia < 100, coli ATCC 8739 (WOCM 00012) Inoculum on reference medium (Salmonella < 100, typhimurium ATCC 14028 (WOCM 00031)) Inoculum on reference medium (Salmonella < 100, centertsis ATCC 13076 (WOCM 00030), Inoculum on reference medium (Salmon < 100, ‘Abony NCTC 6017) Inoculum on reference medium(Cronobacter< 100, ssakazaki ATCC 29544 (WOCMO0214)) Inoculum on reference medium(Cronobacter 100 ‘muyliensii ATCC 51329 (WDCM00213)) Geowth (Escherichia coli ATCC 25922 004 to very good (woe 09013) Growth (Escherichia coll ATCC 8739 (WOCM good to very good 00012)) Growth (Salmonelia typhimurium ATCC. good to very good +4028 (WOCM 00031) Growth (Salmonella entertsis ATCC 13076 good to very goad (won 00030) Growth (Salmonella Abony NCTC 6017) good to very good ‘Growth (Cronobacter sakazakii ATCC 2544 good to very good (woeMo0214)) ‘Growth (Cronobacter muyliensi ATCC 51329 good to very goad (woeMo0213) Incubation: 18+ 2 hours at 37 # 1°C. Cronobacter 18 + 2 hours at 34 - 38 °C S.abony 18-24 hours a 30-35°C Merck KGaA, Frankfurter Strate 250, 64293 Darmstadt (Germany): +49 6151 72.0 Page 1 of 2 EMD Millipore Corporation -a subsidiary of Merck KGaA, Darmstadt, Germany 400 Summit Drive, Burlington, MA 01803, USA, Phone +1 (781) 533-6000Specification 1.07228.5000 Buffered Peptone Water acc. ISO 6579, ISO 19250, ISO 21528, ISO 22964, ISO 6887, FDA-BAM and EP GranuCult ® Test as diluent Specification inoculum on reference medium 100 (Staphylococcus aureus ATCC 25923 (won 09034) Inoculum on reference medium (Escherichia < 100, coli ATCC 28022 (DCM 00013)) Inoculum on reference medium (Escherichia < 100 coli ATCC 6739 (WOCM 00012) Colony count (Staphylococcus aureus ATCC. 25923 (WDCM 00034)) Colony count (Escherichia col ATCC 25922 (woe 09013) Colony count (Escherichia coll ATCC 8739 (wen 09012) Recovery (Staphylococcus aureus ATCC 70-130 % 28023 (wDcw00034) Recovery (Escherichia coll ATCC 25922 70- 190 % (wen 00013) Recovery (Escherichia col ATCC 8739 70-190 % (woe 09012), Incubation: 45 minutes up to 1 hour at 18 ~27 °C (laboratory ambient temperature) Stefanie Fischer Response lboralary manage qally Sona] ‘This document has been praciced electronically and is vali without a signature, Merck KGaA, Frankfurter Strae 250, 64293 Darmstadt (Germany): +49 6151 72.0 Page 2 of 2 EMD Millipore Corporation -a subsidiary of Merck KGaA, Darmstadt, Germany 400 Summit Drive, Burlington, MA 01803, USA, Phone +1 (781) 533-6000