Results

1. Compare your results with your ideal results by making a note and describing any differences you
observe between the results. Explain why the bands ended up in those relative positions. Is this what
you predicted would happen?

-They are opposite to what I had predicted. I believe this is because the yellow band is the smallest and
it travels easiest in the gel, and the blue band travelled least because it is the largest.

2. What part of the procedure do you think had the greatest impact on the results you obtained? What
part of the procedure do you think had the greatest impact on the results you obtained?

- Using the power supply to move the samples.

Reflection

1. 1. Study your gel electrophoresis results. Which sample contained a single dye, and what evidence
from your experiment supports this choice?

- S3, based on the number of the bands.

2. What charge did the dyes in this experiment have?

- They were all negative because they moved toward the positive poles.

3. The dyes you separated in this simulation are orange G (yellow), bromophenol blue (purple) and
xylene cyanole (blue). If these dyes were fragments of DNA, which color dye would represent the
smallest piece of DNA?

- Yellow, because it traveled the farthest.

4. If you compared two molecules that were found in the blue band in the gel, what could you infer
about them?

- They have the same size and charge.

5. If you ran a gel like the one pictured below with wells in the middle, and loaded positively charged
dye molecules into the gel, where would you expect to see a band in the gel?

- A because the positive molecules will travel to the negative pole.

6. How confident do you feel about the technique you learned?

- Fairly confident.