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Analytical Separation Techniques Report Pablo López
Analytical Separation Techniques Report Pablo López
Analytical Separation Techniques Report Pablo López
Santiago de Compostela
Laboratory
report of
Analytical
Separation
Techniques
No, because acetone is miscible with water and for LLE we need two immiscible phases
where the solute is distributed.
A good solvent for LLE must be immiscible with the aqueous phase. It also must not be
too viscous for the extraction and it ought to be stable at work conditions (In this case,
not having a low boiling point at room temperature).
2.- In the example of I2 partitioning between water and carbon disulfide. Why is iodine
much more soluble in the organic phase?
I2 (solute) is a diatomic molecule with net total dipole equal to 0 (it is nonpolar). On the
other hand, we have two solvents, one polar (water) and one nonpolar (carbon
disulfide).
3.- If instead of I2, we would like to extract acetic acid, how would the pH influence
the extraction yield?
The acetic acid is a weak base that in water can be in the neutral and the ionic specie
depending on the pH. If we have and acid media, the concentration of protons [H+] will
increase, and the ionic specie of acid will be lower (to remain constant Ka) than in a basic
media, where the concentration of protons would be lower.
Response
0.8
0.7 y = 0.0343x + 0.0208
R² = 0.9962
0.6
0.5
RESPONSE
0.4
0.3
0.2
0.1
0
0 5 10 15 20 25
CONC
Sample:
Replicate absorbance µg Fe µg Fe/ml
number
1 0,516 14,437 3,610
2 0,548 15,371 3,843
Concentration in the sample:
14,904± 0,660438 µg/mL Fe
Notes:
Equilibrium between chloroform with oxine and the aqueous solution
containing 80 micrograms of Fe(III).