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Applications of Ir Spectroscopy: Presented By: Muhammed Fahad 1 M.Pharm
Applications of Ir Spectroscopy: Presented By: Muhammed Fahad 1 M.Pharm
SPECTROSCOPY
Presented by:
Muhammed Fahad
1st M.Pharm
QUALITATIVE ANALYSIS
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1. Identification of Substances
• To compare spectrums.
• No two samples will have identical IR
spectrum.
• Criteria: Sample and reference must be tested
in identical conditions, like physical state,
temperature, solvent, etc.
• Disadvt: Enantiomers cannot be distinguished
(spectrum are identical).
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The “Fingerprint” Region (1200 to 700 cm-1) :
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4. Detection of Impurities
• Determined by comparing sample spectrum
with the spectrum of pure reference
compound.
• Eg.: ketone impurity in alcohols.
• Detection is favoured when impurity possess a
strong band in IR region where the main
substance do not possess a band.
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5. Isomerism in Organic Chemistry
(i) Geometrical Isomerism:
• trans isomers give a simpler spectrum than
cis due to symmetry.
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Contd…
• E.g.: Ethanol
normal OH – 3636 cm-1
weak band – 3622 cm-1
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(iii) Tautomerism:
Existence of 2 or more chemical compds capable
of intercovertion , usually by exchanging a
hydrogen atom between the 2 atoms.
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6. Functional Group Isomerism
OH = 3500-3100 cm-1
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7. APPLICATIONS OF IR SPECTROSCOPY TO
INORGANIC COMPLEXES
Difficulties:
1. High modes of vibration:
Since they contain more than 5 atoms; hence min. 10
modes of vibrations.
2. Lower symmetry of Complexes:
Due to formation of ligand & polynuclear complexes.
3. Formation of chelates:
Large no. of new bands appear.
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APPLICATIONS OF IR SPECTROSCOPY TO
INORGANIC COMPLEXES
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APPLICATIONS OF IR SPECTROSCOPY TO
INORGANIC COMPLEXES
Geometrical Isomerism:
Eg: Bipyridyl cobalt (III) chloride
[Co(Bipy)2(Cl2)Cl]
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8. Shape of Symmetry of a Molecule
• E.g.: Nitrogen dioxide, NO2
If linear --> only 2 bands should be present.
If bent --> 3 bands should be present.
Actual spectrum shows 3 peaks at 750, 1323 and
1616 cm-1.
• Similarly, IR spectrum was used to determine
structures of XeF2, XeF4 & XeF6 linear, square
planar and octahedral resp.:
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9. Identification of Functional Groups
Due to the presence of functional group region.
E.g.:
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10. Presence of Water in Sample
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11. Measurement of Paints &
Varnishes
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12. Examination of Old Paintings &
Artifacts
• Help to determine fake “masterpieces”.
• Varnish & paints from old items (statues, canvas,
etc.) are analysed by IR spectroscopy.
• Presence of new paint traces implies the
“masterpiece” is fake.
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13. In Industry
1. Determine impurities in raw materials (to ensure
quality products).
2. For Quality Control checks; to determine the %
of required product.
3. Identification of materials made in industrial
research labs,
or materials of competitors.
E.g.: Impurity in bees wax (with petroleum wax)
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14. Analysis of Petroleum HCs, Oil &
Grease contents
• These contain C–H bonds.
Absorption at 3100-2700 cm-1.
• ‘Freons’—Fluorocarbon-113; do not contain C–H
bond.
• Thus, quantity of HCs, oil & grease in freons is
determined by measuring C–H absorption at
2930 cm-1.
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15. Quantitative Analysis of
Multicomponent Mixtures of Sulfur-oxygen
Anions by ATR Spectroscopy
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16. Characterization of Heterogenous
Catalysts by Diffuse Reflectance
Spectroscopy
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17. Analysis of Multilayered Polymeric
Film using FTIR Spectroscopy
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Other Applications
1. Determination of unknown contaminants in
industry using FTIR.
2. Determination of cell walls of mutant & wild
type plant varieties using FTIR.
3. Biomedical studies of human hair to identify
disease states (recent approach).
4. Identify odour & taste components of food.
5. Determine atmospheric pollutants from
atmosphere itself.
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QUANTITATIVE ANALYSIS
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QUANTITATIVE ANALYSIS
• Based on the determination of one of the
functional groups.
E.g.: concn of hexanol in hexane-hexanol mixture.
A = -log I1/I0 = abc (Beer-Lambert’s law)
A = Absorbance
I0 = Intensity of radiation before entering the sample
I1 = Intensity of radiation after leaving the sample
a = Absorptivity of the solution
b = Initial path length of the sample cell
c = concn. of the solution
If ‘b’ & ‘a’ are const., then ‘A’ α ‘c’
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2 methods to determine ‘A’ and conc. ‘c’:
1. Cell-in cell-out Method:
Std. calibration curve method
2. Baseline Method:
selection of suitable absorption band
P0 & P are measured
Abs, log (P0/P) plotted against conc; determine
unknown
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Baseline Method:
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Advantages:
1. Common possible errors are eliminated.
2. Same cell is used for all determinations.
3. All measurements are done on points defined by
the spectrum; hence no dependence on λ
intensity.
4. Eliminate changes in instrument sensitivity and
source intensity.
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Using KBr Pellets (Disk Technique):
Uniform pellets of similar weight & thickness
Known wts. of KBR + known qty of test
Calibration curve plotted
Disks are weighed and thickness measured
Using Internal Std. (pot. thiocyanate):
Dried, ground with KBr to make a conc of 0.2% by wt
of thiocyanate.
Calibration curve plotted.
Ratio of thiocyanate absorption at 2125 cm-1 to a
chosen band of test is plotted vs conc.
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