MICROBIAL GROWTH SIMULATION WORKSHEET

(Salmonella spp.): Aerobic growth

A. Please complete the Table. From the data you get, does the temperature affect the growth kinetics?
Which factors that are affected by the change of the temperature? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 6.5
103 105 25 0.5 6.5
103 105 20 0.5 6.5
103 105 15 0.5 6.5
103 105 10 0.5 6.5

B. Please complete the Table. From the data you get, does the salt content affect the growth kinetics?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 6.5
103 105 30 1.5 6.5
103 105 30 2.5 6.5
103 105 30 3.5 6.5
103 105 30 4.5 6.5

C. Please complete the Table. From the data you get, does the pH affect the growth kinetics?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 5.6
103 105 30 0.5 6.0
103 105 30 0.5 6.4
103 105 30 0.5 6.6
103 105 30 0.5 6.8

D. Please complete the Table. From the data you get, does the salt content affect the growth kinetics at low temperature?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 10 0.5 6.5
103 105 10 1 6.5
103 105 10 2 6.5
103 105 10 3 6.5
103 105 10 4 6.5

E. Please complete the Table. From the data you get, does the pH affect the growth kinetics at low temperature?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 10 0.5 5.6
103 105 10 0.5 6.0
103 105 10 0.5 6.4
103 105 10 0.5 6.6
103 105 10 0.5 6.8

F. Please complete the Table. From the data you get, does the initial levels affect the growth kinetics?
Which factors that are affected by the change of the initial levels? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 109 30 0.5 6.5
104 109 30 0.5 6.5
105 109 30 0.5 6.5
106 109 30 0.5 6.5
107 109 30 0.5 6.5
 MICROBIAL GROWTH SIMULATION WORKSHEET
(Escherichia coli O157:H7): Aerobic growth

A. Please complete the Table. From the data you get, does the temperature affect the growth kinetics?
Which factors that are affected by the change of the temperature? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 40 0.5 6.5
103 105 30 0.5 6.5
103 105 20 0.5 6.5
103 105 15 0.5 6.5
103 105 10 0.5 6.5

B. Please complete the Table. From the data you get, does the salt content affect the growth kinetics?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 1 6.5 2 0.5 2.2 9.37 24.50

103 105 30 2 6.5 1.7 0,6 3.1 9.25 24.50

103 105 30 3 6.5 1.43 0.7 4.7 8.94 24.50

103 105 30 4 6.5 1.25 0.8 7.6 9.37 49.00

103 105 30 5 6.5 1.0 1.0 13.3 9.23 49.00

C. Please complete the Table. From the data you get, does the pH affect the growth kinetics?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 4.5
103 105 30 0.5 5
103 105 30 0.5 6
103 105 30 0.5 7
103 105 30 0.5 8

D. Please complete the Table. From the data you get, does the salt content affect the growth kinetics at low temperature?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 10 1 6.5
 Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 10 2 6.5
103 105 10 3 6.5
103 105 10 4 6.5
103 105 10 5 6.5

E. Please complete the Table. From the data you get, does the pH affect the growth kinetics at low temperature?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 10 0.5 4.5
103 105 10 0.5 5
103 105 10 0.5 6
103 105 10 0.5 7
103 105 10 0.5 8

F. Please complete the Table. From the data you get, does the initial levels affect the growth kinetics?
Which factors that are affected by the change of the initial levels? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 109 30 0.5 6.5
104 109 30 0.5 6.5
105 109 30 0.5 6.5
106 109 30 0.5 6.5
107 109 30 0.5 6.5

MICROBIAL GROWTH SIMULATION WORKSHEET

(Escherichia coli O157:H7): Anerobic growth

A. Please complete the Table. From the data you get, does the temperature affect the growth kinetics?
Which factors that are affected by the change of the temperature? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 40 0.5 6.5
103 105 30 0.5 6.5
103 105 20 0.5 6.5
103 105 15 0.5 6.5
103 105 10 0.5 6.5

B. Please complete the Table. From the data you get, does the salt content affect the growth kinetics?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 1 6.5
103 105 30 2 6.5
103 105 30 3 6.5
103 105 30 4 6.5
103 105 30 5 6.5

C. Please complete the Table. From the data you get, does the pH affect the growth kinetics?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 4.5
103 105 30 0.5 5
103 105 30 0.5 6
103 105 30 0.5 7
103 105 30 0.5 8

D. Please complete the Table. From the data you get, does the salt content affect the growth kinetics at low temperature?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
 Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
Count (°C) Content (h) (h) (h)
103 105 10 1 6.5
103 105 10 2 6.5
103 105 10 3 6.5
103 105 10 4 6.5
103 105 10 5 6.5

E. Please complete the Table. From the data you get, does the pH affect the growth kinetics at low temperature?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 10 0.5 4.5
103 105 10 0.5 5
103 105 10 0.5 6
103 105 10 0.5 7
103 105 10 0.5 8

F. Please complete the Table. From the data you get, does the initial levels affect the growth kinetics?
Which factors that are affected by the change of the initial levels? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 108 30 0.5 6.5
104 108 30 0.5 6.5
105 108 30 0.5 6.5
106 108 30 0.5 6.5
107 108 30 0.5 6.5

MICROBIAL GROWTH SIMULATION WORKSHEET

(Staphylococcus aureus): Aerobic growth

A. Please complete the Table. From the data you get, does the temperature affect the growth kinetics?
Which factors that are affected by the change of the temperature? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 40 0.5 6.5
103 105 35 0.5 6.5
103 105 30 0.5 6.5
103 105 25 0.5 6.5
103 105 20 0.5 6.5

B. Please complete the Table. From the data you get, does the salt content affect the growth kinetics?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 2 6.5
103 105 30 4 6.5
103 105 30 6 6.5
103 105 30 8 6.5
103 105 30 10 6.5

C. Please complete the Table. From the data you get, does the pH affect the growth kinetics?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 5
103 105 30 0.5 6
103 105 30 0.5 7
103 105 30 0.5 8
103 105 30 0.5 9

D. Please complete the Table. From the data you get, does the salt content affect the growth kinetics at low temperature?
 Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 15 2 6.5
103 105 15 4 6.5
103 105 15 6 6.5
103 105 15 8 6.5
103 105 15 10 6.5

E. Please complete the Table. From the data you get, does the pH affect the growth kinetics at low temperature?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 15 0.5 5
103 105 15 0.5 6
103 105 15 0.5 7
103 105 15 0.5 8
103 105 15 0.5 9

F. Please complete the Table. From the data you get, does the initial levels affect the growth kinetics?
Which factors that are affected by the change of the initial levels? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 109 30 0.5 6.5
104 109 30 0.5 6.5
105 109 30 0.5 6.5
106 109 30 0.5 6.5
107 109 30 0.5 6.5

MICROBIAL GROWTH SIMULATION WORKSHEET

(Staphylococcus aureus): Anaerobic growth

A. Please complete the Table. From the data you get, does the temperature affect the growth kinetics?
Which factors that are affected by the change of the temperature? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 40 0.5 6.5
103 105 35 0.5 6.5
103 105 30 0.5 6.5
103 105 25 0.5 6.5
103 105 20 0.5 6.5

B. Please complete the Table. From the data you get, does the salt content affect the growth kinetics?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 2 6.5
103 105 30 4 6.5
103 105 30 6 6.5
103 105 30 8 6.5
103 105 30 10 6.5

C. Please complete the Table. From the data you get, does the pH affect the growth kinetics?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 5
103 105 30 0.5 6
103 105 30 0.5 7
103 105 30 0.5 8
103 105 30 0.5 9
 Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)

D. Please complete the Table. From the data you get, does the salt content affect the growth kinetics at low temperature?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 15 2 6.5
103 105 15 4 6.5
103 105 15 6 6.5
103 105 15 8 6.5
103 105 15 10 6.5

E. Please complete the Table. From the data you get, does the pH affect the growth kinetics at low temperature?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 15 0.5 5
103 105 15 0.5 6
103 105 15 0.5 7
103 105 15 0.5 8
103 105 15 0.5 9

F. Please complete the Table. From the data you get, does the initial levels affect the growth kinetics?
Which factors that are affected by the change of the initial levels? Please give explanation

Initial Leve of Storage Product’s Product’s Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 108 30 0.5 6.5
104 108 30 0.5 6.5
105 108 30 0.5 6.5
106 108 30 0.5 6.5
107 108 30 0.5 6.5

MICROBIAL GROWTH SIMULATION WORKSHEET

(B. cereus): Aerobic growth

A. Please complete the Table. From the data you get, does the temperature affect the growth kinetics?
Which factors that are affected by the change of the temperature? Please give explanation

Initial Leve of Storage Product’s Product’s Na-nitrit Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH (μg/ml) Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 40 0.5 7 0
103 105 30 0.5 7 0
103 105 20 0.5 7 0
103 105 15 0.5 7 0
103 105 10 0.5 7 0

B. Please complete the Table. From the data you get, does the salt content affect the growth kinetics?
Which factors that are affected by the change of the salt content? Please give explanation

Initial Leve of Storage Product’s Product’s Na-nitrit Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH (μg/ml) Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 1 7 0
103 105 30 2 7 0
103 105 30 3 7 0
103 105 30 4 7 0
103 105 30 5 7 0

C. Please complete the Table. From the data you get, does the pH affect the growth kinetics?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Na-nitrit Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH (μg/ml) Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 5.0 0
103 105 30 0.5 5.5 0
103 105 30 0.5 6.0 0
103 105 30 0.5 6.5 0
 Initial Leve of Storage Product’s Product’s Na-nitrit Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH (μg/ml) Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 7.0 0

D. Please complete the Table. From the data you get, does the Na-nitrit affect the growth kinetics?
Which factors that are affected by the change of the Na-nitrit? Please give explanation

Initial Leve of Storage Product’s Product’s Na-nitrit Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH (μg/ml) Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 30 0.5 7 0
103 105 30 0.5 7 30
103 105 30 0.5 7 60
103 105 30 0.5 7 90
103 105 30 0.5 7 120

E. Please complete the Table. From the data you get, does the pH affect the growth kinetics at low temperature?
Which factors that are affected by the change of the pH? Please give explanation

Initial Leve of Storage Product’s Product’s Na-nitrit Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH (μg/ml) Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 105 10 0.5 5.0 0
103 105 10 0.5 5.5 0
103 105 10 0.5 6.0 0
103 105 10 0.5 6.5 0
103 105 10 0.5 7.0 0

F. Please complete the Table. From the data you get, does the initial levels affect the growth kinetics?
Which factors that are affected by the change of the initial levels? Please give explanation

Initial Leve of Storage Product’s Product’s Na-nitrit Growth Generation Lag phase Maximum Time required to Image
Microbial concern Temp NaCl pH (μg/ml) Rate time time population reach final count
Count (°C) Content (h) (h) (h)
103 108 30 0.5 7 0
104 108 30 0.5 7 0
105 108 30 0.5 7 0
106 108 30 0.5 7 0
107 108 30 0.5 7 0