( 404 ) ‫توصيف مقرر دراسي‬

Course Specifications (MB 303)

2009/2010
‫ بيانات المقرر‬.1
Basic Information
/ ‫الفرقة‬ :Title)) ‫أسم المقرر‬ ) ‫الرمز الكودي‬
:‫المستوي‬ Cell Differentiation :(Code
(Academic year / Level)
.3rd / B.Sc and Tissue Culture MB 303
‫عملي‬ ‫تمارين‬ ‫نظري‬ ‫عدد‬
.Lab .Tout .Lect ‫الوحدات‬ ) ‫التخصص‬
:‫الدراسية‬ :(Specialization
3 - 1 Number of
Molecular Biology
Units

1- To provide insights into the cell differentiation and to

understand that it is a terminal stage where cells
irreversibly lose their proliferative capacity which
represents a form of negative control of growing.
2- to illustrate the ability of stem cells to differentiate, unlike
specialised cells with examples of differentiation-inducing
and -inhibiting factors.
3- To cover basic procedures of cell and tissue culture and ‫ اهداف المقرر‬.2
providing knowledge of tissue culture methods including:
types of cells grown in culture, work area and equipment, Aims of Course
preservation and storage, maintenance of cell lines, safety
consideration, and procedures
4- To illustrate the requirement of aseptic conditions, solid
surface, growth factors and nutrients in complex growth
media.
5- Providing knowledge of Protocols specific to individual
tissue types as microcosms.

‫المستهدف من تدريس المقرر‬.3

(Intended Learning Outcomes (ILOs
1- The knowledge that the process whereby relatively ‫ المعلومات والمفاهيم‬.1
unspecialized cells, e.g. embryonic or regenerative cells, Knowledge and
withdraw from the cell cycle and acquire specialized Understanding
structural and/or functional features that characterize the
cells, tissues, or organs of the mature organism.
Differentiation includes the processes involved in
commitment of a cell to a specific fate and its subsequent
development to the mature state.
2- Understand the aim of mammalian cell culture is to
provide an environment that mimics, to the greatest extent
possible, the in vivo environment of that specific cell type
 by providing an appropriate temperature, pH, oxygen, and
CO2 supply, surface for cell attachment, nutrient and
vitamin supply, protection from toxic agents, and the
hormones and growth factors that control the cell's state of
growth and differentiation.
3- The knowledge about types of cell culture and
proliferation versus differentiation
4- The understanding of the difficulty in maintaining cultures
of mammalian cells due to cells dying after a finite number
of divisions in culture.
5- the understanding of the considerable commercial
importance of cell culture in many biotechnology and
pharmaceutical companies.
1- Ability to discuss recent studies using cultured cells.
2- The students will learn the ability to correlate between
different biological samples and show the importance of
different media in tissue culture
3- Ability to evaluate ideas and concepts.
4- Ability to recognise and acknowledge inconsistencies in an ‫ المهارات الذهنية‬.2
argument. Intellectual Skills
5- Ability to question and challenge viewpoints, ideas and
concepts.
6- Make judgments about the value of evidence, concepts and
ideas.
1- The ability to acquire basic skills to characterize cell
cultures, sterilization technique, preparation of cell culture
medium, quantification of cells in culture.
2- Evaluate experimental procedures in situations that are
unfamiliar, by commenting on the purpose or approach,
the suitability and effectiveness of procedures, the control ‫ المهارات المهنية‬.‫ج‬
of variables, the limitations of equipment, possible sources ‫الخاصة بالمقرر‬
of error and/or suggestions for improvement. Professional and
3- The ability to evaluate and synthesize complex research practical Skills
material.
4- Plan and design experimental procedures to test given
hypotheses or to illustrate particular effects. This could
include identification of variables, controls and
measurements or observations required.
1- Work in a team and communicate orally, written, or by ‫ المهارات العامة‬.‫د‬
visual means.. General and
2- Make predictions and generalisations based on available Transferable Skills
evidence.
3- Demonstrate interpersonal skills and personal
effectiveness of planning, time management; writing up on
time; handling relationships with supervisors.
4- Show competence in the use of information technology
(IT) and information handling.
5- Extract of knowledge from the text books and webs that
help in construct the objectives and strategies.
6- Have self confidence and leadership skills
7- Utilize modern techniques of presentation.

- Comparison of features and ultrastructure of prokaryotic

and eukaryotic cells.
- Cell growth and Control of the cell cycle.
- Abnormal cell division: cancer cells.
- Differentiation of cells into tissues and organs.
- Animal Cell Culture: Historical Background and Theory of
Tissue Culture
- Setting Up a Cell Culture Laboratory
- Primary Culture and Establishing Cell Lines ‫ محتوى المقرر‬.4
- Importance of Serum and Serum Free Media Course content
- Standard Cell Culture Techniques
- Looking at Cells
- Contamination: How to Avoid It, Recognize It, and Get Rid
of It
- Mycoplasma: Detection and Control
- Monoclonal Antibody Production
- Insect Cell Culture: An Overview
- Cell Culture for Commercial Settings

Lectures and text books 5. ‫أساليب التعليم والتعلم‬

Power point presentation Teaching and Learning Methods

N/A
‫ أساليب التعليم والتعلم للطلب ذوي الحتياجات الخاصة‬.6
Teaching and Learning Methods of Disables

:‫ تقويم الطلب‬.7
Student Assessment
- Mid & End term quiz
- Final practical exam :‫ الساليب المستخدمة‬.1
- Final-term written exam Student Assessment Methods
- Mid & End term quiz...Week 7&14
- Final practical exam…. Week15 ‫ التوقيت‬.2
- Final written exam……. Week 16 Assessment Schedule

Mid & End term quiz 10

Final practical exam 30
Oral exam 10 ‫ توزيع الدرجات‬.‫ج‬
Final-term written exam 50 Weighting of Assessments
Other types of assessment 0.0
Total 100

8. ‫قائمة الكتب الدراسية أو المراجع‬:

List of References
- Lecture notes ‫ مذكرات‬.1
Course Note
- Culture of Animal Cells: A manual of basic
technique, 5th Edition. (2005) Freshney RI.
WIELY-LISS. ISBN: 978-0-471-45329-1. ‫ كتب ملزمة‬.2
- Introduction to Cell and Tissue Culture: Required Books
Theory and Technique. Jennie P. Mather and (Text Books)
Penelope E. Roberts (1998) Plenum Press, New
York. ISBN 0-306-45859-4
- Animal Cell Culture: A practical approach,
2nd edition. (1992) Freshney, R.I, ,Oxford
University Press.
- Cell Commitment and Differentiation. Norman
Maclean and Brian Keith Hall (1987) Cambridge ‫ كتب مقترحة‬.‫ج‬
University Press. ISBN 0-521-30884-4. Recommended Books
- Handbook of growth factors, Volume 1 by
Enrique Pimentel (1994) CRC Press, Inc. ISBN
0-8493-2505-6.
----- ‫ألخ‬...‫ دوريات علمية أو نشرات‬.‫د‬
.Periodicals, web sites, …etc

External references for standards (Benchmarks):

This programme is designed to meet The ‫ المعايير الكاديمية‬.9
national reference academic standards NARS
standards set by the national quality assurance Academic standards
and accreditation agency NQAAA or appropriate
standards.

‫رئيس مجلس القسم العلمي‬: :‫أستاذ المادة‬

‫مدير البرنامج‬: