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Efficacy of The Product of Molasses Grass (Melinis Minutiflora) As A Delaying Agent On Ripening Mangoes (Mangifera Indica)
Efficacy of The Product of Molasses Grass (Melinis Minutiflora) As A Delaying Agent On Ripening Mangoes (Mangifera Indica)
Researchers
Alina Gumarang
Andrea Barasi
Vincel Vicente
Fritzie Talosig
Janinah Barba
Irvin Acosta
Adviser:
Mrs. Jhonaliza Padilla
Title page 1
Table of contents 2
Abstract 4
CHAPTER I - INTRODUCTION
Hypothesis 10
Conceptual Framework 11
Definition of Molasses 12
Types of Molasses 13
Ethylene 14
Experimental Unit 16
Materials 17
Uses of Materials 17
Procedure 18
Research Design 18
Data Gathering 18
Table of Treatments 19
Table of Results 20
Summary of Findings 21
Conclusion 21
Recommendations 22
Bibliography 23
Acknowledgment 24
Appendices 25
ABSTRACT
Fruits do not have a long shelf life and when over ripe
results to spoilage making it inedible to eat. So the purpose of
this study is to test the efficacy of molasses as a delaying
agent on ripening mangoes.
This proves that the molasses can delay the ripening and
prolong the shelf life of the fruits.
CHAPTER I
BACKGROUND OF THE STUDY
Fresh fruits are perishable. Main problems on fruits are over
ripening and disease development. Fresh fruits do not have a long
shelf life but with proper storage, we can keep it from going
ripe soon. Ripening is a normal phase in the maturation process
wherein changes such as enhanced color, flavor, aroma and any
other changes occurs in fruits and vegetables. When plants
encounter ethylene, a gas they produce naturally as a hormone,
the result is softening and ripening in the case of fruits and
wilting and fading in the case of flowers. A lot of studies have
been conducted in the speeding up of the ripening and the bearing
of fruits that are not in season. These studies that have been
established were proven to be of great help. On the contrary, our
project is to delay the ripening by testing the efficacy of
molasses on the climacteric fruit: mango.
Fruits and vegetables can be preserved by canning, freezing,
drying, or by the use of chemical preservatives. By these
methods, the fruits and vegetables are killed and preserved from
microbiological spoilage either by initial sterilization and
protection from subsequent re-infection, or by maintaining
conditions which do not allow the growth of microorganisms. Each
of these methods of preservation presents special microbiological
problems but they will not be considered here.
The bulk of the fruits and vegetables used for food are
handled in the fresh state, for, though highly perishable, they
normally remain at ordinary temperatures in good condition for at
least one to two days, and in some instances, as long as one or
two months; by taking suitable precautions, such as refrigerated
storage, the storage period can be increased, though only to a
limited extent.
They must be preserved from spoilage by microorganisms. It
is impossible to consider these two aspects entirely separately
since changing physiological condition of the fruit or vegetable
results in changing susceptibility to microbiological attack.
Microbiological spoilage is primarily due, not to the
multiplication of bacteria yeasts or molds on a non-living
nutrient medium-though in some instance multiplication or growth
on the surface maybe a secondary cause of loss-but to the
invasion of living tissue. It is for this reason that control of
attack involves special difficulties and that the experimental
study the rotting of fruits and vegetables has special points of
interests.
The mango is a remarkable fruit. When harvested at optimum
storage potential, it may store for as long as 12 months under
proper temperature and atmospheric conditions. Correct harvest
timing is based on defining the stage of maturity of the mangoes,
which maybe best determined by measuring fruit ethylene
biosynthesis. Ethylene was discovered in the late 1800’s. Like
many other discoveries, it was somewhat accidental. Someone had
observed several trees along a tree-lined boulevard had lost
their leaves. It was soon discover that there was a leak in one
of the underground pipes carrying natural gas. It didn’t take
long to learn ethylene was the causative agent. Since then,
hundreds of papers have been written on ethylene action,
biosynthesis promotion, inhibition, and molecular genetics.
Ethylene is found in most living tissues. In most
terrestrial mammals, small amounts of ethylene are expressed in
every exhale breath, but in animals, ethylene is not considered a
hormone as it is in plants. There are seven major effects of
ethylene in plants which are: promoting ripening, inducing fruit
abscission, inducing flowering, promoting seed germination, break
dormancy, promoting root initiation and inducing vegetative
dwarfing. The science of fruit growing, most of these effects are
put to use for the grower’s benefit. This paper gives an overview
of ethylene’s involvement in the process of fruit ripening, how
it is measured, and what the measurements mean.
Ethylene production is largely pre-determined in both time
and amount by the genetics of the genetics of the fruit and ,
depending on when the fruit blossoms and the climate, will
determine to a large degree the development of the respiratory
climacteric. During fruit development, respiration (i.e., the
generation of carbon dioxide), which is a measure of metabolic
activity, declines gradually throughout the season until several
weeks before it ripens where it reaches what is known as a pre-
climacteric minimum. At this point, the metabolic functions of
the fruit are in near resting stage in preparation for a burst of
metabolic activity signifying ripening. During ripening, both
carbon dioxide and ethylene increase significantly. The main
developmental stage of the fruit is referred as maturation, in
which photosynthetic is converted to starch. The ripening phase
is when the starch is converted to sugar. Senescence is the stage
in which the membrane functions breakdown due to the gradation of
lipid bilayers leading to sell damage and necrosis.
Optimum harvest is a subjective measurement defined as a
good keeping quality and good eating quality. If picked too early
during the maturation stage, insufficient starch will be
converted to sugar and the fruit will keep well enough, but the
eating quality will be poor. On the other hand, if the fruit is
picked too late, there will be insufficient starch and acid
reserves for metabolic maintenance in storage, but the eating
quality will be good. Therefore, the optimum timing is critical
to the proper storage (and marketing) of mangoes. Researchers
throughout the world have spent decades defining the optimum time
of harvest for long-term storage and yet, as new varieties enter
the marketplace, optimums must be redefined for each individual
cultivar.
Both the practical agricultural use of ethylene (C2H4), and
the basic biochemistry and physiology of C2H4 have been
extensively studied for many decades (abeles et a., 1992).
Elucidation of the C2H4 biosynthetic pathway by Adams and Yang
(1979) and the recent application of molecular biology to unravel
the complexities of C2H4 biosynthesis and action have greatly
stimulated research in this area (Yang, 1985; DellaPenna and
Giovannoni, 1991; Grierson and Schuch, 1994; Kanellis et al.,
1997). However, much of what is known about the effects of C 2H4
on the quality of fresh fruits and vegetables has been slowly
amassed since the 1920’s, and the needs constant updating. The
introduction of new cultural practices, cultivars, harvest and
handling methods, postharvest treatments, consumer products and
packaging influence the effect of C2H4 is based. The information
presented in this review has been gleaned from recent
publications and from past reviews on the biochemistry nad
physiology of C2H4, its role in postharvest handling, and its
effect on food quality. Ethylene is a naturally produce, simple
two carbon gaseous plant growth regulator that has numerous
effects on the growth, development and storage life of many
fruits, vegetables and ornamental crops. This powerful plant
hormone is effective at part-per-million to part-per-billion
concentrations. Both the synthesis and action of C2H4 involved
complicated metabolic processes, which require oxygen and are
sensitive to elevated concentrations of carbon dioxide.
Climacteric fruits refer to fruits that have high
respiration rate during the fruits ripening. During the ripening
process of the climacteric fruits, the production of
phytohormone, ethylene, dramatically increase up to one thousand
fold of the basal ethylene level. Climacteric fruits are the ones
that are still able to ripen even after being picked. An example
of climacteric fruit is they are picked and shipped green and
then ripen at a later time in the store or home.
Some plants have been utilized as an edible cover to disrupt
interaction between the fruit and the environment. For it has
been known that environmental factors can also trigger the
speeding up of ripening of fruits.
Molasses is a viscous by product of the processing of
sugarcane grapes or sugar beets into sugar also a brownish
residue left after crystallization of sucrose will be used as a
protectant to prevent any reactions of the fruits from the
environment. The glucose component of the molasses also contains
hydroploxyl cellulose, and either cellulose in which some
hydroxyl groups in the repeating units have hydroxylpropylated
forming. HPC is used as a tropical ophthalmic protectant and
lubricant.
STATEMENT OF THE PROBLEM
The study generally aims to determine the capability of molasses
as a delaying agent on mangoes. Specifically, aims to answer the
following questions:
1. Do molasses have the capability to delay the ripening of
mangoes?
2. Is there a significant difference of the results between the
group given 50% molasses from the group given 100% molasses?
3. Will the treatments effect in the delaying of the ripening
of mangoes?
4. Are mangoes the best experimental unit for molasses as a
delaying agent?
5. Can water help in the delaying the process of ripening?
6. Can the factors of molasses prove itself?
7. What are the other factors of molasses that can delay
ripening?
8. Can the natural ripening process be better than the delayed
one?
9. Will the percentage of molasses given to each fruit change
its firmness, aroma, and texture?
10. Is molasses really effective and helpful for fruit
vendors?
HYPOTHESIS
Null hypothesis:
There is no significant difference of the results between the
group given 50% molasses and 100% molasses.
Alternative hypothesis:
Molasses can delay the ripening of mangoes.
CONCEPTUAL FRAMEWORK
INDEPENDENT VARIABLE: DEPENDENT VARIABLE:
Chapter II
REVIEW OF RELATED LITERATURE
Black treacle
Blackstrap molasses
Golden Syrup
Sorghum
Antidote
Glucose
Maple Syrup
Cane molasses
Sugarbeet molasses
Unsulphured molasses
1. Variety
2. Maturity at time of harvest
3. Storage temperature
4. Physical damage
Meanwhile, delaying agents are mostly used in climacteric fruits.
Climacteric fruits are those which undergo a ‘ripening phase’
associated with increase respiration and ethylene production,
softening, composition change, color changes, and aroma
production. The management and control of fruit ripening is
important for the successful transport of fresh fruits and
vegetable. Fruit that ripen too early are easily damaged during
transport and produce ethylene which can adversely affect other
commodities. The technique used to delay and/or manage ripening
include:
Cold storage
Controlled atmosphere storage
Ethylene addition and/or removal
Inhibition of ethylene action through chemical means.
OTHER USES:
CHEMICAL
Experimental Unit
CHAPTER III
METHDOLOGY
This chapter presents the following procedures and details during
the experiment. It will also show the needed materials in the
experiment.
MATERIALS:
A. Procedure
1. First, ten (10) mangoes are needed to conduct the experiment;
these are divided into five (5) groups.
2. The researchers put two (2) mangoes in each container. The
researchers put 25% molasses and 75% water on the first group.
3. The second group was filled with 50% molasses and 50% water
while the third group is filled with 75% of molasses and 25%
water. The fourth group was completely covered with 100% molasses
without water. Lastly, the last group without any treatment. The
container will be tightly sealed and put in a room temperature.
4. The experimental units will be stored for five days. After
that, the researchers will be able to compare the results
A. Research design
The research design of this study will be randomized complete
block design. Composing of ten (10) experimental units divided
into five (5) groups. These groups are assigned with different
treatments; varying concentrations of molasses. 25% on the first
group, 50% on the second group, 75% on the third group, and 100%
on the fourth group and no treatments on the last group.
B. Data gathering
Data would be gathered through their physical aspects; the
colour, firmness and taste. Researchers will gather the
acceptability. The assistance of the Department of Agriculture
will aid the researchers on the measurement scale of the result
of the study.
D 2 100% -
E 2 - -
D 2 100% -
E 2 - -
D 2 100% -
E 2 - -
CHAPTER IV
Legends:
1 = Yellow green
3 = green
Legends:
1 = firm
2 = soft
3 = softer
Legends:
1 = mild
3 = inedible/ rotten
TREATMENT 1 TREATMENT 2 TREATMENT 3 TREATMENT 4 TREATMENT 5
25% - 75% 50% - 50% 75% - 25% 100% NATURAL
A 1 1 1 1 3
B 1 1 1 1 3
CHAPTER V
The results show at the first table the color appearance of the
mangoes. The control group has a rating of 1 which implies
turning yellow green. Meantime, the second table shows the
ratings of firmness of the mangoes. The control group has a
rating of 2 meaning soft. Most of the groups with treatments have
a rating of 1 making it different with the natural one. Table 3
shows different ratings in terms of its taste. The control group
when opened is considered inedible and rotten. The first
replicate of the group given with 25% treatment is rated mild.
The group with 75% molasses shows different results. The first
replicate of the group has a mild taste. This now shows that the
best treatment out of all is the 50%-50% because it passes all
the qualities needed . Meaning molasses really delay the ripening
of mangoes and there is a significant difference between the
group given with 25%,50%,and 75% treatment with the group given
with 100% molasses.
Conclusion
Recommendations
BIBLIOGRAPHY
www.wordhippo.com/molasses
www.organicfacts.net/molasses grass
nutritiodata.self.com/mangoes
invescience.com/effects of molasses on climacteric fruits
postharvest.free.wsu.edu.com/climacteric fruits
nutrition-and-you.com/benefits of molasses
En.wikipedia.org/melinis minutiflora
ACKNOWLEDGMENT
APPENDICES
Each treatment.
treatment.
2 mangoes.
6. Leave the mangoes fully soaked