TECHNOLOGICAL INSTITUTE OF THE PHILIPPINES

938 Aurora Blvd., Cubao, Quezon City

COLLEGE OF ENGINEERING AND ARCHITECTURE

Department of Environmental and Sanitary Engineering

ENSE 504
ENVIRONMENTAL ENGINEERING LABORATORY

LABORATORY REPORT #2

DETERMINATION OF DISSOLVED OXYGEN IN WATER

TITLE

PANGILINAN, PHILIP P.
Student

OCTOBER 15, 2020

Date Submitted

AMIR Y. PASCUAL
Instructor

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 EXPERIMENT NO. 2

DETERMINATION OF DISSOLVED OXYGEN IN WATER

Intended Learning Objective:

To Estimate Dissolved Oxygen (DO) level in the given water sample by
Winkler’s Method

Principle:
The major inputs of Dissolved Oxygen (DO) to natural water are from
atmosphere and photosynthetic reaction. Where the algae and
phytoplankton production is high, the saturation of oxygen can occur during
daytime. The solubility of oxygen in water depends on temperature and
other factors. The presence of oxygen is essential for the survival of aquatic
life in water. A rapid fall of DO in river water is one of the first indications of
pollution.

Significance:

It is necessary to know the DO level to assess quality of raw water and to

keep a check on stream pollution.
 A minimum DO of 4 to 5 mg/l is desirable for the survival of aquatic
life.
 Higher values of DO may cause corrosion of Iron and steel.>
 DO test is used to evaluate the pollution strength of domestic and
industrial wastes.

Winkler’s Modified Method:

The Winkler’s Method is the technique used to measure DO in freshwater

structures. It is used as an indicator of the health of a water body, where
higher DO concentrations are associated with high production and little
contamination. This test is performed on-site, as delays between sample
collections and testing may result in a variation in oxygen content.

In this method of analysis the oxygen present in the water sample oxidizes
the divalent manganous ion to its higher valency, which precipitates as a
brown hydrated oxide after addition of sodium hydroxide (NaOH) and
potassium iodide (KI). Upon acidification, manganese reverts to divalent
state and liberates iodine from KI equivalent to DO content in the sample.
The liberated iodine is titrated against sodium thiosulphate (0.025N), using
freshly prepared 2% starch solution as indicator. If the oxygen is absent in
the sample, the MnSO4 react with the alkali to form white precipitate
Mn(OH)2.

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APPARATUS:

1. BOD BOTTLES
2. PIPETTE
3. CONICAL FLASK
4. BURETTE
5. BEAKER
6. DROPPER
7. SPATULA

REAGENT:

1. WATER
2. SODIUM THIOSULFATE SOLUTION

PROCEDURE:

1. Fill a burette with sodium thiosulfate (Na2S2O3) solution

2. Take 10ml of 0.025N Potassium Dichromate (K2Cr2O7) in a conical
flask
3. Add 2ml of Hydrochloric acid (HCL) and 1 spatula of potassium iodide
(KI) powder to the same conical flask
4. Titrate the sample against (Na2S2O3). Add 2ml starch when the color of
the solution changes to pale yellow. Then continue titration until the
blue color becomes colorless
5. Close the knob when the color of solution in conical flask changes to
pale yellow
6. Fill BOD bottle with sample water up to the bottle brim
7. Immediately add 2ml of manganese sulfate (MnSO4) solution to the
BOD bottle by inserting the automatic pipette just below the surface of
the liquid
8. Add 2ml of alkali – iodide – azide reagent to the BOD bottle inserting
the automatic pipette just below the surface of the liquid
9. Mix the sample by inverting BOD bottle several times and allow the
precipitate to settle down at the bottom
10. Add 2ml of concentrated sulfuric acid (H2SO4) via graduated
pipette carefully without forming air bubbles
11. Take 200ml of sample in a conical flask using pipette
12. Titrate the sample against Na2S2O3. Add 2ml starch when the
color of the solution changes to pale yellow. Then continue the titration
until blue color becomes colorless
13. Close the knob when the color of the solution changes to
colorless

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OBSERVATIONS:

RESULTS:

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DETERMINATION OF DISSOLVED OXYGEN:

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 l ul ted issolved gen mg

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CONCLUSION:

I therefore conclude that the calculated result of dissolved oxygen which is

7.08 mg/L is beyond the limit prescribed under DAO 2016 – 08 which is
5mg/L for water classified as AA for drinkable water source. Thus, it is not
safe to drink.

RECOMMENDATION:

Other international standard aside from ours which is the DAO 2016 -08 has
an acceptable limit of Dissolved Oxygen in water from 4mg/L to 7mg/L.
Less than the allowable limit is detrimental to health, so I recommend a
further water purification process must take place before engaging to human
consumption.

REFERENCES:

1. DAO 08 - 2016 WATER QUALITY GUIDELINES AND GENERAL

EFFLUENT STANDARDS OF 2016
2. DAO 34 – 1990 REVISED WATER USAGE AND
CLASSIFICATION/WATER QUALITY CRITERIA AMENDING SECTION
NOS. 68 AND 69, CHAPTER III OF THE 1978 NPCC RULES AND
REGULATIONS

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“I affirm that I have not given or received any unauthorized help on this
assignment, and that this work is my own.

Philip P. Pangilinan
BS ENSE
1820215

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