Ankita Choudhury

ankitasrm31@gmail.com

+91 6294203099

www.linkedin.com/in/ankita-choudhury1994

EDUCATION

Course University GPA Year

MSc. Stem Cell Technology and Manipal Academy of Higher Education 8.59 2017-2019
Regenerative medicine

B.Tech Genetic Engineering SRM University 7.84 2012-2016

INTERNSHIP/ DISSERTATION

• BIOCON RESEARCH LIMITED (January- May, 2020) - Research Intern (Molecular Biology)
Project: Cloning of Heavy chain and Light chain of Monoclonal Antibodies in engineered vectors for
increased secretion of the antibodies
Techniques:
- Plasmid DNA isolation (Mini prep and Mega prep)
- Restriction Digestion
- Ligation
- Gel elution
- Infusion Cloning
- Agarose gel electrophoresis
- Transformation

• MANIPAL INSTITUTE OF REGENERATIVE MEDICINE, BANGALORE (September,2018-

June, 2019) - Masters Dissertation
Project: Inhibition of the Triple Negative Breast Cancer (TNBC) Cell line, MDA-MB-231, by the Wnt
Suppressor LGK974
Guidance: Dr. Sudha Warrier (MIRM-MAHE)
Techniques:
- Molecular biology techniques- RNA isolation, cDNA synthesis, Polymerase Chain Reaction (PCR), Real
time PCR, Protein isolation, SDS PAGE, Western blotting, Immunofluorescence, ELISA
- Assays- Bradford Assay, MTT Assay, ROS Assay, Casapase 3/7 Assay
- Cell culture- Mammalian cell culture- Breast cancer cell line and Cancer Stem cell culture
• UNIVERSITY OF ZURICH, SWITZERLAND (January- June, 2016)- Bachelor’s dissertation
Project: Effects of SLC16A12 Cataract-associated mutations on function and localization of its
MCT12 protein
Guidance: Dr. Barbara Kloeckener Gruissem
Techniques:
- PCR
- Plasmid DNA isolation
- Invitro Transcription
- Sanger Sequencing
- Micro-injection of RNA in frog oocytes
- Radioactive Creatinine uptake assay

• INSTITUTE OF GENETIC ENGINEEERING,KOLKATA (June-July, 2014)- Intern

Project: Diagnosis of HbE Haemoglobinopathies in Eastern-Indian population
Techniques:
- Liver Function tests (bilirubin estimation, SGOT(Serum Glutamate Oxaloacetate Transaminase),
SGPT(Serum Glutamate Pyruvate Transaminase) and AMP (Alkaline phosphatase estimation).
- ARMS-PCR
- Renal function tests (estimation of creatinine, urea and uric acid)

• INDO-AMERICAN HYBRID SEEDS PVT LTD.,BANGALORE (December,2013)-Intern

Title: Basics of Plant tissue culture techniques
Techniques:
- Explant culture
- In-vitro seed culture

ACTIVITIES

• CO-CURRICULAR ACTIVITIES :
- Attended Indian Genetics Congress, March- 2015, Chennai, Tamil Nadu, India
- Attended International Symposium on Genomics in Health and Disease, (ISHG) January 2015,
Mumbai, Maharashtra
• EXTRA-CURRICULAR ACTIVITIES :
- Committee member for a forensic biology based event, two years in a row Aaruush (Techno-
Management fest) 2013 and 2014.
- Participant in the event Robogyan (Robotics Workshop), in Aarush 2012.
- Volunteer for Rare Disease Day, 2014. A program for spreading awareness about rare diseases and the
lives of people affected by it, organized by Dept. of Genetic Engg., SRM University.
PERSONAL SKILLS AND ACHIEVEMENTS
- Topper of Batch 2017-2019 in MSc. Stem Technology and Regenerative Medicine in Manipal
Academy of Higher Education (MAHE)
- First Position in Women’s badminton, cricket and Second position in Volleyball in Sports Day, MAHE
- Professional Kathak Dancer

PERSONAL INTERESTS
- Dancing
- Swimming

LANGUAGES
- Bengali- Native
- English - Fluent
- Hindi- Fluent

PERSONAL INFORMATION
- Date of Birth- 31.10.1994
- Sex- Female
- Nationality- Indian

I hereby declare that all the above facts and figures are correct to my knowledge.