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Increasing your profitability:

Saving cost, time and footpath with the fantastic doubled throughput HPLC

Hendy Dwi Warmiko

Sales Support SPV
PT GeneCraft Labs

The world leader in serving science

 PT GeneCraft Labs

CHONS Analyzer

IRMS

2
Thermo Scientific Vanquish - Our Promise

Client

If a company could help with

productivity,
in terms of instrumentation, service, uptime and support,
then we would go with that company.
-Patheon

3
Overcoming Customer Pain Points
COMPLEMENTARY
ASSAYS

HIGH THROUGHPUT

BENCH SPACE

LEARNING
SOFTWARE MEANINGFUL
RESULTS

RETURN ON INVESTMENT

4
Analytical Challenge – Expectation

Accelerate time to results Need for efficient characterization

Increased number of Increased complexity of

samples samples

Process more samples Need for complementary

with decreasing number characterization methods
of resources

Asked to do more with less Asked to know more with less

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Analytical Challenges – Time

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The First and Best Dual LC in the market

Vanquish HPLC
UltiMate 3000 HPLC

7
Key Benefits – Thermo Scientific Vanquish System for Tandem LC or LC-MS
Advanced column wash without sacrificing throughput
100
Eluent B [%]

50 Standard gradient profile

0
0 10 20 30 40 50 60
100 Run Time [min]
Eluent B [%]

50 Time savings Tandem mode allows for time savings

0
0 10 20 30 40 50 60
Run Time [min]
100 Additional column wash
Eluent B [%]

50
Time savings Extra column washing steps without
sacrificing throughput
0
0 10 20 30 40 50 60
Run Time [min]

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Tandem LC or LC-MS Technical Realization

9
Tandem LC

10
Key Benefits – Thermo Scientific Vanquish System for Tandem LC or LC-MS
Easy to implement
1. 2. 3.

Automatic instrument recognition

and fluidic knowledge
Standard instrument method
setup Automatic method setup for
analytical and
reconditioning pump

11
Technical Realization – Dual LC / Parallel LC

12
Any pump combination possible

Full application flexibility due to separated fluidics

13
Key Benefits – Thermo Scientific Vanquish System for Dual LC
Easy-to-use as any other Thermo Scientific™ Vanquish™ system
1. 2. 3.
Simple instrument selection

Easy method setup with

method wizard

Automatic method translation

14
Key Benefits – Thermo Scientific Vanquish System for Dual LC

Two different samples and applications

15
Thermo Scientific Vanquish Modules

Thermo Scientific™ Vanquish™ Thermo Scientific™ Vanquish™

Dual Pump Dual Split Samplers

 2 ternary pumps in one housing  2 injection units in one autosampler

 700 and 1000 bar version  Independent flow paths
 Delivers 2 completely independent
gradients

16
Key Benefits – Vanquish for Dual LC
No additional bench space needed
Vanquish for Dual LC system Vanquish system

55 cm 55 cm

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What Can Be Accomplished with Thermo Scientific Vanquish System for Dual LC?

“The instrument offers a fantastic increase in throughput

when running two different separation chemistries, enabling us to
generate large amounts of characterization data”

Dr. Jonathan Bones, NIBRT

Finished in half the time Efficient characterization

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Analysis of water and fat soluble vitamins

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Key Benefits – Thermo Scientific Vanquish System for Tandem LC or LC-MS
Lower costs per sample
Cost of ownership calculator:
Throughput increase
Cost per sample

Number of Samples Per Day

48

Throughput and
31
costs comparison
24 between standard
single and Vanquish
Duo workflows

Long Gradient experimental conditions Single Dual Tandem

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Key Benefits – Thermo Scientific Vanquish System for Dual LC
Lower costs per sample
Cost of ownership calculator:
Throughput increase
Cost per sample

Number of Samples Per Day

192

Throughput gain
Doubled
96 throughput and
lower costs per
sample

Aggregate analysis experimental conditions Single Dual

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Analytical Challenge – Thermo Scientific Vanquish System for Inverse Gradient

Need to detect any

compound in a
sample, even
without
chromophore

Individual standards for

each compound required
for quantitative
measurements

Asked to improve detection and quantification capabilities

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High Sensitivity Detection
High detector sensitivity

Evaporative Light Scattering Detector Charged Aerosol Detector

Theophylline Caffeine Theophylline Caffeine
Actual LOD
8 ng o. c. 16 ng o. c. 0.5 ng o. c. 4 ng o. c.
S/N ≥3

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 Inverse Gradient Compensation for Uniform Response

Conventional Gradient Elution

Analytical Gradient
85%

Eluent at detector
Identical mass o.c.
Eluent dependent
analyte response
15% 15%

Analytical Gradient 85%

Inverse Gradient Compensation

Eluent at detector
15% 15%
50%

85% 85%

Inverse Gradient
15%

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Thermo Scientific Vanquish UHPLC for Inverse Gradient

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Key Benefits – Thermo Scientific Vanquish System for Inverse Gradient
Easy to implement
1. 2. 3.

Automatic instrument recognition

and fluidic knowledge

Standard instrument method setup

Automatic method setup for
compensation gradient

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Key Benefits – Thermo Scientific Vanquish UHPLC System for Inverse Gradient
Standard free quantification

Without gradient Compensation With Inverse Gradient Compensation

0.4 Adenine 0.4 Adenine

Tenofovir
Emtricitabin Tenofovir
0.3
Peak Area (pA*min)

Peak Area (pA*min)

Tenofovir Disoproxil 0.3
Emtricitabine

0.2 0.2 Tenofovir Disoproxil

0.1 0.1

0.0 0
0 10 20 30 40 50 0 10 20 30 40 50
Mass on Column (ng) Mass on Column (ng)

Similar response curves for all analytes after gradient compensation

27
28
New Thermo Scientific Vanquish Modules Overview

User Interface
• Intuitively monitor system Solvent Monitor
status & health • Monitors solvent &
• View maintenance videos waste level of up to
8 solvents
• Prediction of estimated
solvent consumption
Detectors
DAD, MWD, VWD, FLD
• Highly linear DAD & MWD Autosampler
• Sensitive VWD & FLD • Cooled or non-cooled
• CAD & ISQ optional additions • Up to 1 mL injection
volume
• Liquid handling
Pumps
LPG, HPG, DGP, ISO
• Automatic purging Column Compartment
• Up to 85 °C
• Optional active pre-
heating

29
Thermo Scientific Vanquish Core System Delivers

Added
safety
features
Blend in with Cost & time
existing routine savings
Precise &
dependable
results

Higher
Improved
sequence
user
success
experience
rate

Full compliance

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Scheduled System Health Checks

System installation Schedule test Cycle & plan Set & forget

System in use Care-free operation

Tests running Notifies Review results

System idle
in background you if necessary w/ build-in report

Minimizes Unplanned Downtime

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Thermo Scientific Vanquish User Interface • Home Screen

Vanquish System Health Status

Controller Information

Fully Compliant
Navigation Bar

Module Information
Maintenance Videos

Easy Access to Instrument Parameters

32
Thermo Scientific Vanquish User Interface • System Diagnostics

Available tests • Chromeleon CDS 7.3

Basic Lamp Shutter Grating

tightness intensity motor motor
test test test test

Benefits

Less downtime

Accelerated troubleshooting & system

performance checks after maintenance & repair

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Uptime is Key to Productivity
a

+25 % +5 min Thermo Scientific™

Health quicker column

Checks
less
interruptions
change Viper™ Fingertight
Fittings

+25 % +10 min

faster saved checking
troubleshooting status/ system
Guided User
Diagnostics Interface

+10 % +0.25 min

sequence faster runtime/
Solvent success rate sample Prepare
Monitoring Next Injection
*)For details refer to WP73373

Get More Work Done with Vanquish Core

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Instrument Robustness

Long-term stability of results Inter-system

More than 170 injections – 3 days 24/7 operation reproducibility
800 mAU

700 Impurity A Thiopental 8 systems in 7 labs

RT RSD 0.06% RT RSD 0.07%
600 Peak Area RSD 0.34% Peak Area RSD 0.06% around the globe
500 Impurity C
RT RSD 0.07%
400

300

200

100

0
min
0 2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0

Excellent long-term retention time All systems passed SST

& area reproducibility with remarkable reproducibility

Robust Workhorse for Routine Labs • Everyday Confidence

35
Thermo Scientific Chromeleon 7.3 and Vanquish Core: Unmatched Productivity and Ease-of-compliance

Chromeleon 7.3 CDS and Vanquish Core HPLC

• Easier adherence to data integrity regulations
• Faster and easier Review of Audit-Trails
• Intuitive analysis of result changes with side-by-side result
comparison

• Superior Lab Productivity and Highest Uptime

• Vanquish Solvent Monitor
• SW guided Method Translation & Method Transfer
• Analyze and resolve common instrument problems with new
instrument diagnostics
• Reduce unplanned downtime with scheduled Health Checks

• Standard Instrument Integration (SII) based control in

3rd party CDS:
• Empower & others

36
Conclusion

37
Thermo Scientific AppsLab Library of Analytical Applications

Search applications
• Filter based on
instrument, analyte or
technique

Find, download, andeWorkflow

Download run
• Hundreds of eWorkflows
new methods in minutes

Run the eWorkflow

in Thermo Scientific™
Chromeleon™ CDS
thermofisher.com/appslab
38
Thermo Scientific Vanquish UHPLC Platform

39
Thermo Scientific Analytical LC Portfolio Overview
Routine Analysis Method Development Research

Thermo Scientific
Vanquish Horizon
UHPLC System
Thermo Scientific
Vanquish Flex UHPLC
Thermo Scientific™ System
Thermo Scientific UltiMate™ 3000 RS/
Vanquish Core HPLC BioRS System
Thermo Scientific™ System
Thermo Scientific™ UltiMate™ 3000 SD
UltiMate™ 3000 Basic System
Automated System

620 bar 700 bar 1000 bar 1500 bar

40
Thermo Scientific Vanquish System: Innovation in Every Aspect

41
Online webpages

1. Vanquish Systems Webpage

2. Vanquish Brochure

3. Cost of Ownership Calculator

42
Application note & references

43
44