Information for Contributors

Journal of Medical Entomology is published in January, March, May, July, September, and November. The journal publishes reports on all
Journal of Volume 53, Number 6
phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of pub-
Medical Entomology November 2016 lic health and veterinary significance. The journal is divided into the following sections: Morphology, Systematics, Evolution; Sampling, Distri-
bution, Dispersal; Development, Life History; Population and Community Ecology; Behavior, Chemical Ecology; Population Biology/
EDITOR-IN-CHIEF RICHARD N. JOHNSON DINA M. FONSECA PAULA M. SHREWSBURY Genetics; Molecular Biology/Genomics; Neurobiology, Physiology, Biochemistry; Vector Control, Pest Management, Resistance, Repellents;
WILLIAM K. REISEN Ellicott City, MD New Brunswick, NJ College Park, MD Arthropod/Host Interaction, Immunity; Vector/Pathogen/Host Interaction, Transmission; Vector-Borne Diseases, Surveillance, Prevention;
Davis, CA Medical, Urban, and Veterinary Eastern Branch Direct Injury, Myiasis, Forensics; Modeling/GIS, Risk Assessment, Economic Impact. In addition to full-length research papers, Journal of
PHILLIP E. KAUFMAN Entomology
LIVY H. WILLIAMS III Medical Entomology publishes interpretive articles in a Forum section, Short Communications, and Rapid Communications. The same guide-
SUBJECT EDITORS Gainesville, FL Montferrier sur Lez, France lines apply to these as to research articles. For more detailed instructions concerning the preparation and submission of manuscripts, visit
THEODORE G. ANDREADIS DANA NAYDUCH
Manhattan, KS International Branch http://jme.oxfordjournals.org/for_authors/index.html.
New Haven, CT TIMOTHY J. LYSYK
Medical, Urban, and Veterinary All manuscripts are reviewed by scientists qualified to judge the validity of the research. Acceptance or rejection, however, is the decision of
Lethbridge, AB, Canada Entomology GARY J. BREWER
ROBERTO BARRERA Lincoln, NE the subject editor. Acceptance of manuscripts is based solely on their scientific merit. Appeal of a rejected manuscript should be made to the
San Juan, PR North Central Branch Editorial Board, via the ESA director of publications. Accepted manuscripts are published approximately in the order they are received.
KEVIN R. MACALUSO REBECCA TROUT FRYXELL
Baton Rouge, LA Manuscript Submission and Preparation. All manuscripts for the journal should be submitted electronically using the online submission sys-
Knoxville, TN DOUGLAS B. WALSH
LYRIC C. BARTHOLOMAY Medical, Urban, and Veterinary tem, ScholarOne. Go to http://mc.manuscriptcentral.com/medent. All authors submitting through ScholarOne for the first time are required to
Madison, WI Prosser, WA
DOUGLAS E. NORRIS Entomology set up an account by filling in their contact information and determining their username and password. Once you have done so, please follow
Pacific Branch
Baltimore, MD the instructions for submitting your manuscript online. Tables and figures should be included as part of the manuscript file. Authors can subse-
BRENDA T. BEERNTSEN JENNIFER ZASPEL TIMOTHY D. SCHOWALTER quently log on to ScholarOne and see the status of their manuscript(s) in their Author Centre. If you have questions, try using the online Help
Columbia, MO SCOTT A. RITCHIE West Lafayette, IN Baton Rouge, LA button first. Or, you can email pubs@entsoc.org or call 301-731-4535, ext. 3020. Submit your manuscript as an MS Word or WordPerfect file
Smithfield, Queensland, Systematics, Evolution, and Southeastern Branch (PC version recommended) with a page size of 8.5 ! 11 inches. Type all as double-spaced, with 1-inch margins, and do not justify text. Use the
JASON H. BYRD Australia Biodiversity
DAVID W. RAGSDALE font Times (New) Roman (12 point). Use italicization only to indicate scientific names, symbols for variables, and words that are defined. Use
Daytona Beach, FL
ESA DIRECTOR OF College Station, TX quotation marks for quoted material only. Use American English spelling throughout and follow Merriam-Webster’s New Collegiate Diction-
HARRY M. SAVAGE Southwestern Branch ary, 10th ed., for guidance on spelling. Number pages consecutively, beginning with the title page. Note that ESA requires that line numbers
PUBLICATIONS
JONATHAN F. DAY Fort Collins, CO
LISA JUNKER be used on your submitted manuscript. Begin each of the following on a separate page and arrange in the following order: title page, abstract
Vero Beach, FL CHRISTOPHER J. GEDEN
Annapolis, MD and key words (three to five words), text, acknowledgments, references cited, footnotes, tables, figure legends, and figures. Type all captions
PATRICIA Y. SCARAFFIA Gainesville, FL
MARIA A. DIUK-WASSER New Orleans, LA Medical, Urban, and Veterinary on a separate page and put each figure and table on a separate page.
PRODUCTION EDITOR
New Haven, CT LORI WILSON Entomology Title Page. In the upper right corner, type the complete name, address, telephone number, fax number, and email of the author who is to
RICHARD C. WILKERSON Cary, NC receive and approve page proofs. In the upper left corner, type Journal of Medical Entomology, the section of the journal in which the manu-
LARS EISEN CAROL M. ANELLI script should appear, and the running head. Use a brief and informative title that includes order and family names in parentheses. Four lines
Silver Spring, MD Columbus, OH
Fort Collins, CO ESA GOVERNING BOARD beneath the title, type in capital letters the complete names of all authors. Four lines beneath the authors’ names, type the department, institu-
Physiology, Biochemistry, and
GUIYUN YAN SUSAN J. WELLER tion, city, state, zip code, and country (other than the United States) of the institution where the research was done. Use numbered footnotes
REBECCA J. EISEN Toxicology
Irvine, CA Minneapolis, MN for authors not affiliated with the research institution, and give their addresses of affiliations on the footnote page.
Fort Collins, CO President SUJAYA RAO Abstract. Abstract should be an informative digest of the significant contents and the main conclusions of the research.
Corvallis, OR References Cited. Citations in the text should be by last name of the author and date and refer to a reference listed alphabetically by author
DAVID A. FLORIN EDITORIAL BOARD MICHAEL P. PARRELLA
ANDREW Y. LI, Plant-Insect Ecosystems
Silver Spring, MD Davis, CA in References Cited. For further formatting instructions, see http://jme.oxfordjournals.org/for_authors/index.html.
Chair Vice President Tables. Keep tables to a minimum. Type all parts of each table double spaced, with each table starting on a new page.
Beltsville, MD JOHN D. OSWALD
LANE D. FOIL College Station, TX Figures. Figures should be submitted as separate files from the manuscript. Figures should be submitted in one of the following formats:
Medical, Urban, and Veterinary ROBERT K. PETERSON
Baton Rouge, LA Entomology Systematics, Evolution, and TIFF, EPS, RTP, DOC, PPT, XLS, PDF, PSD, PS, AI, GIF, PNG, or WMF.
Bozeman, MT
Biodiversity Image resolution should be 300 pixels per inch and figures should be one or two columns wide (i.e., 72 or 148 mm). Separate parts of the
CHRISTOPHER J. GEDEN Vice President-Elect
AQEEL AHMAD same figure must be grouped together and arranged to use space efficiently. Wherever possible, it is best to avoid using a full page for a set of
Gainesville, FL LINA BERNAOLA illustrations. That is, authors should attempt to have each figure appear separately from the other and should consider numbering illustrations
Saint Louis, MO MAY R. BERENBAUM
Plant-Insect Ecosystems Urbana, IL Baton Rouge, LA as separate figures rather than as multiple parts of the same figure. Authors should indicate a preference for one- or two-column reproduction,
HOWARD S. GINSBERG Past President Student Representative
and are asked to account for proportionate reduction or enlargement in the choice of lettering size, type style, and the format of the artwork
Kingston, RI
JOSHUA B. BENOIT itself. Final lettering size should be 8 or 9 point, using the font Arial or Helvetica. Authors with questions concerning the preparation of art-
Cincinnati, OH MICHELLE S. SMITH C. DAVID GAMMEL
work can refer to the Oxford Journals website at http://www.oxfordjournals.org/en/authors/figures.html or contact the publications office
RICARDO E. GÜRTLER Physiology, Biochemistry, and Indianapolis, IN Annapolis, MD
Buenos Aires, Argentina Toxicology Treasurer Executive Director staff at 301-731-4535 or pubs@entsoc.org.
Style Conventions. When first mentioned, a plant or animal should include the full scientific name and the author of the zoological name
unabbreviated, except for Linnaeus (L.) and Fabricius (F.). Use only common names approved by the Entomological Society of America
(http://www.entsoc.org/pubs/common_names/index.htm). Otherwise, the manuscript should conform to the guidelines established by the lat-
Cover: Chrysomya putoria female. Image courtesy of Nina Parry, Flies of Economic Significance Research Group, Department of Zoology & Entomology, University est edition of the Council of Science Editors Style Manual.
of Pretoria (see pp. 1322–1329).
Proofs. Page proofs are sent to authors after manuscripts are typeset. Extensive changes to proofs, except for printer and editorial errors and
answers to copyeditor queries addressing style or format, will be charged to the author.
Editorial Charges. Editorial review charges are assessed on all accepted manuscripts to cover some of the expenses of publishing. See the
website listed below for current rates. Reprints (printed and electronic) are ordered at the time page proofs are returned. See the reprint order
form for rates. Authors who are members of ESA at the time their article is published are exempt from page charges related to their article.
For more information, see the Journal of Medical Entomology website at http://jme.oxfordjournals.org.

JME-53-6-Cover.indd 2 10-11-2016 19:05:06

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Publication Dates
Volume 53, Issue 5 Published Online: September 3, 2016; Print Issue Mailed: September 19, 2016
 Information for Contributors
Journal of Medical Entomology is published in January, March, May, July, September, and November. The journal publishes reports on all
Journal of Volume 53, Number 6
phases of medical entomology and medical acarology, including the systematics and biology of insects, acarines, and other arthropods of pub-
Medical Entomology November 2016 lic health and veterinary significance. The journal is divided into the following sections: Morphology, Systematics, Evolution; Sampling, Distri-
bution, Dispersal; Development, Life History; Population and Community Ecology; Behavior, Chemical Ecology; Population Biology/
EDITOR-IN-CHIEF RICHARD N. JOHNSON DINA M. FONSECA PAULA M. SHREWSBURY Genetics; Molecular Biology/Genomics; Neurobiology, Physiology, Biochemistry; Vector Control, Pest Management, Resistance, Repellents;
WILLIAM K. REISEN Ellicott City, MD New Brunswick, NJ College Park, MD Arthropod/Host Interaction, Immunity; Vector/Pathogen/Host Interaction, Transmission; Vector-Borne Diseases, Surveillance, Prevention;
Davis, CA Medical, Urban, and Veterinary Eastern Branch Direct Injury, Myiasis, Forensics; Modeling/GIS, Risk Assessment, Economic Impact. In addition to full-length research papers, Journal of
PHILLIP E. KAUFMAN Entomology
LIVY H. WILLIAMS III Medical Entomology publishes interpretive articles in a Forum section, Short Communications, and Rapid Communications. The same guide-
SUBJECT EDITORS Gainesville, FL Montferrier sur Lez, France lines apply to these as to research articles. For more detailed instructions concerning the preparation and submission of manuscripts, visit
THEODORE G. ANDREADIS DANA NAYDUCH
Manhattan, KS International Branch http://jme.oxfordjournals.org/for_authors/index.html.
New Haven, CT TIMOTHY J. LYSYK
Medical, Urban, and Veterinary All manuscripts are reviewed by scientists qualified to judge the validity of the research. Acceptance or rejection, however, is the decision of
Lethbridge, AB, Canada Entomology GARY J. BREWER
ROBERTO BARRERA Lincoln, NE the subject editor. Acceptance of manuscripts is based solely on their scientific merit. Appeal of a rejected manuscript should be made to the
San Juan, PR North Central Branch Editorial Board, via the ESA director of publications. Accepted manuscripts are published approximately in the order they are received.
KEVIN R. MACALUSO REBECCA TROUT FRYXELL
Baton Rouge, LA Manuscript Submission and Preparation. All manuscripts for the journal should be submitted electronically using the online submission sys-
Knoxville, TN DOUGLAS B. WALSH
LYRIC C. BARTHOLOMAY Medical, Urban, and Veterinary tem, ScholarOne. Go to http://mc.manuscriptcentral.com/medent. All authors submitting through ScholarOne for the first time are required to
Madison, WI Prosser, WA
DOUGLAS E. NORRIS Entomology set up an account by filling in their contact information and determining their username and password. Once you have done so, please follow
Pacific Branch
Baltimore, MD the instructions for submitting your manuscript online. Tables and figures should be included as part of the manuscript file. Authors can subse-
BRENDA T. BEERNTSEN JENNIFER ZASPEL TIMOTHY D. SCHOWALTER quently log on to ScholarOne and see the status of their manuscript(s) in their Author Centre. If you have questions, try using the online Help
Columbia, MO SCOTT A. RITCHIE West Lafayette, IN Baton Rouge, LA button first. Or, you can email pubs@entsoc.org or call 301-731-4535, ext. 3020. Submit your manuscript as an MS Word or WordPerfect file
Smithfield, Queensland, Systematics, Evolution, and Southeastern Branch (PC version recommended) with a page size of 8.5 ! 11 inches. Type all as double-spaced, with 1-inch margins, and do not justify text. Use the
JASON H. BYRD Australia Biodiversity
DAVID W. RAGSDALE font Times (New) Roman (12 point). Use italicization only to indicate scientific names, symbols for variables, and words that are defined. Use
Daytona Beach, FL
ESA DIRECTOR OF College Station, TX quotation marks for quoted material only. Use American English spelling throughout and follow Merriam-Webster’s New Collegiate Diction-
HARRY M. SAVAGE Southwestern Branch ary, 10th ed., for guidance on spelling. Number pages consecutively, beginning with the title page. Note that ESA requires that line numbers
PUBLICATIONS
JONATHAN F. DAY Fort Collins, CO
LISA JUNKER be used on your submitted manuscript. Begin each of the following on a separate page and arrange in the following order: title page, abstract
Vero Beach, FL CHRISTOPHER J. GEDEN
Annapolis, MD and key words (three to five words), text, acknowledgments, references cited, footnotes, tables, figure legends, and figures. Type all captions
PATRICIA Y. SCARAFFIA Gainesville, FL
MARIA A. DIUK-WASSER New Orleans, LA Medical, Urban, and Veterinary on a separate page and put each figure and table on a separate page.
PRODUCTION EDITOR
New Haven, CT LORI WILSON Entomology Title Page. In the upper right corner, type the complete name, address, telephone number, fax number, and email of the author who is to
RICHARD C. WILKERSON Cary, NC receive and approve page proofs. In the upper left corner, type Journal of Medical Entomology, the section of the journal in which the manu-
LARS EISEN CAROL M. ANELLI script should appear, and the running head. Use a brief and informative title that includes order and family names in parentheses. Four lines
Silver Spring, MD Columbus, OH
Fort Collins, CO ESA GOVERNING BOARD beneath the title, type in capital letters the complete names of all authors. Four lines beneath the authors’ names, type the department, institu-
Physiology, Biochemistry, and
GUIYUN YAN SUSAN J. WELLER tion, city, state, zip code, and country (other than the United States) of the institution where the research was done. Use numbered footnotes
REBECCA J. EISEN Toxicology
Irvine, CA Minneapolis, MN for authors not affiliated with the research institution, and give their addresses of affiliations on the footnote page.
Fort Collins, CO President SUJAYA RAO Abstract. Abstract should be an informative digest of the significant contents and the main conclusions of the research.
Corvallis, OR References Cited. Citations in the text should be by last name of the author and date and refer to a reference listed alphabetically by author
DAVID A. FLORIN EDITORIAL BOARD MICHAEL P. PARRELLA
ANDREW Y. LI, Plant-Insect Ecosystems
Silver Spring, MD Davis, CA in References Cited. For further formatting instructions, see http://jme.oxfordjournals.org/for_authors/index.html.
Chair Vice President Tables. Keep tables to a minimum. Type all parts of each table double spaced, with each table starting on a new page.
Beltsville, MD JOHN D. OSWALD
LANE D. FOIL College Station, TX Figures. Figures should be submitted as separate files from the manuscript. Figures should be submitted in one of the following formats:
Medical, Urban, and Veterinary ROBERT K. PETERSON
Baton Rouge, LA Entomology Systematics, Evolution, and TIFF, EPS, RTP, DOC, PPT, XLS, PDF, PSD, PS, AI, GIF, PNG, or WMF.
Bozeman, MT
Biodiversity Image resolution should be 300 pixels per inch and figures should be one or two columns wide (i.e., 72 or 148 mm). Separate parts of the
CHRISTOPHER J. GEDEN Vice President-Elect
AQEEL AHMAD same figure must be grouped together and arranged to use space efficiently. Wherever possible, it is best to avoid using a full page for a set of
Gainesville, FL LINA BERNAOLA illustrations. That is, authors should attempt to have each figure appear separately from the other and should consider numbering illustrations
Saint Louis, MO MAY R. BERENBAUM
Plant-Insect Ecosystems Urbana, IL Baton Rouge, LA as separate figures rather than as multiple parts of the same figure. Authors should indicate a preference for one- or two-column reproduction,
HOWARD S. GINSBERG Past President Student Representative
and are asked to account for proportionate reduction or enlargement in the choice of lettering size, type style, and the format of the artwork
Kingston, RI
JOSHUA B. BENOIT itself. Final lettering size should be 8 or 9 point, using the font Arial or Helvetica. Authors with questions concerning the preparation of art-
Cincinnati, OH MICHELLE S. SMITH C. DAVID GAMMEL
work can refer to the Oxford Journals website at http://www.oxfordjournals.org/en/authors/figures.html or contact the publications office
RICARDO E. GÜRTLER Physiology, Biochemistry, and Indianapolis, IN Annapolis, MD
Buenos Aires, Argentina Toxicology Treasurer Executive Director staff at 301-731-4535 or pubs@entsoc.org.
Style Conventions. When first mentioned, a plant or animal should include the full scientific name and the author of the zoological name
unabbreviated, except for Linnaeus (L.) and Fabricius (F.). Use only common names approved by the Entomological Society of America
(http://www.entsoc.org/pubs/common_names/index.htm). Otherwise, the manuscript should conform to the guidelines established by the lat-
Cover: Chrysomya putoria female. Image courtesy of Nina Parry, Flies of Economic Significance Research Group, Department of Zoology & Entomology, University est edition of the Council of Science Editors Style Manual.
of Pretoria (see pp. 1322–1329).
Proofs. Page proofs are sent to authors after manuscripts are typeset. Extensive changes to proofs, except for printer and editorial errors and
answers to copyeditor queries addressing style or format, will be charged to the author.
Editorial Charges. Editorial review charges are assessed on all accepted manuscripts to cover some of the expenses of publishing. See the
website listed below for current rates. Reprints (printed and electronic) are ordered at the time page proofs are returned. See the reprint order
form for rates. Authors who are members of ESA at the time their article is published are exempt from page charges related to their article.
For more information, see the Journal of Medical Entomology website at http://jme.oxfordjournals.org.

JME-53-6-Cover.indd 2 10-11-2016 19:05:06

JOURNAL OF MEDICAL ENTOMOLOGY
VOLUME 53, NUMBER 6
NOVEMBER 2016
CONTENTS

LETTER TO THE EDITOR

Resolving Confusion in the Use of Concepts and Terminology in Intrapuparial Development Studies of
Cyclorrhaphous Diptera
Daniel Martı́n-Vega, Martin J. R. Hall, and Thomas J. Simonsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1249

ARTHROPOD/HOST INTERACTION, IMMUNITY

Effect of Amblyomma maculatum (Acari: Ixodidae) Saliva on the Acute Cutaneous Immune Response to
Rickettsia parkeri Infection in a Murine Model
K. H. Banajee, V. I. Verhoeve, E. K. Harris, and K. R. Macaluso . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1252

BEHAVIOR, CHEMICAL ECOLOGY

Presence of Putative Male-Produced Sex Pheromone in Lutzomyia cruciata (Diptera: Psychodidae),
Vector of Leishmania mexicana
A. K. Serrano, J. C. Rojas, L. C. Cruz-López, Edi A. Malo, O. F. Mikery, and A. Castillo . . . . . . . . . . . . . . . . . . . . 1261

DEVELOPMENT, LIFE HISTORY

Evaluating the Biological Cycle and Reproductive and Population Parameters of Calliphora vicina
(Diptera: Calliphoridae) Reared on Three Different Diets
Cindy Pérez, Nidya A. Segura, Manuel A. Patarroyo, and Felio J. Bello . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1268

DIRECT INJURY, MYIASIS, FORENSICS

First Report of Necrophagous Insects on Human Corpses in Riyadh, Saudi Arabia
R. A. Alajmi, H. AlGhufaili, A. Farrukh, H. Aljohani, and A.M.A. Mashaly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1276

Action of Ants on Vertebrate Carcasses and Blow Flies (Calliphoridae)

Michele C. Paula, Gustavo M. Morishita, Carolina H. Cavarson, Cristiano R. Gonçalves,
Paulo R. A. Tavares, Angélica Mendonça, Yzel R. Súarez, and William F. Antonialli-Junior . . . . . . . . . . . . . . . 1283

MODELING/GIS, RISK ASSESSMENT, ECONOMIC IMPACT

A Density Map of the Tick-Borne Encephalitis and Lyme Borreliosis Vector Ixodes ricinus
(Acari: Ixodidae) for Germany
Katharina Brugger, Denise Boehnke, Trevor Petney, Gerhard Dobler, Martin Pfeffer, Cornelia Silaghi,
Günter A. Schaub, Beate Pinior, Hans Dautel, Olaf Kahl, Kurt Pfister, Jochen Süss, and Franz Rubel . . . . . 1292

MORPHOLOGY, SYSTEMATICS, EVOLUTION

Revalidation of Nyssomyia fraihai (Martins, Falcão & Silva 1979) (Diptera: Psychodidae)
R. E. Godoy and E. A. B. Galati . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1303

First Records of the Syringophilid Mites (Acariformes: Prostigmata) Parasitizing Ibises and Spoonbills
(Pelecaniformes: Threskiornithidae), With Description of Four New Species
Maciej Skoracki, Mateusz Zmudzinski, Markus Unsoeld, and Bozena Sikora . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1312

POPULATION AND COMMUNITY ECOLOGY

Seasonal, Locality, and Habitat Variation in Assemblages of Carrion-Associated Diptera in Gauteng
Province, South Africa
N. J. Parry, M. W. Mansell, and C. W. Weldon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1322

SAMPLING, DISTRIBUTION, DISPERSAL

Mosquitoes (Diptera: Culicidae) From the Northwestern Brazilian Amazon: Padauari River
R. S. G. Hutchings, R. W. Hutchings, I. S. Menezes, M. de A. Motta, and M. A. M. Sallum . . . . . . . . . . . . . . . . 1330

Composition and Genetic Diversity of Mosquitoes (Diptera: Culicidae) on Islands and Mainland Shores
of Kenya’s Lakes Victoria and Baringo
Yvonne Ukamaka Ajamma, Jandouwe Villinger, David Omondi, Daisy Salifu, Thomas Ogao Onchuru,
Laban Njoroge, Anne W. T. Muigai, and Daniel K. Masiga . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1348
The Arrival of the Northern House Mosquito Culex pipiens (Diptera: Culicidae) on Newfoundland’s
Avalon Peninsula
Andrew C. Chaulk, Kate P. Carson, Hugh G. Whitney, Dina M. Fonseca, and Thomas W. Chapman . . . . . . 1364

Protein Self-Marking by Ectoparasites: A Case Study Using Bed Bugs (Hemiptera: Cimicidae)
Frances S. Sivakoff, Susan C. Jones, Scott A. Machtley, and James R. Hagler . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1370

Mosquito Capture Rate Using CO2-Baited Traps in Relation to Distance From Water and Height:
Implications for Avian Disease Transmission
Oliver Eshun, Alec Gerry, and William K. Hayes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1378

Coexistence of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Peninsular Florida Two
Decades After Competitive Displacements
L. Philip Lounibos, Irka Bargielowski, Marı́a Cristina Carrasquilla, and Naoya Nishimura . . . . . . . . . . . . . . . . . 1385

New Records of Ticks (Acari: Ixodidae) From Dogs, Cats, Humans, and Some Wild Vertebrates in
Alaska: Invasion Potential
Lance A. Durden, Kimberlee B. Beckmen, and Robert F. Gerlach . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1391

VECTOR CONTROL, PEST MANAGEMENT, RESISTANCE, REPELLENTS

Control of Hyalomma lusitanicum (Acari: Ixodidade) Ticks Infesting Oryctolagus cuniculus
(Lagomorpha: Leporidae) Using the Entomopathogenic Fungus Beauveria bassiana (Hyocreales:
Clavicipitaceae) in Field Conditions
J. González, F. Valcárcel, J. L. Pérez-Sánchez, J. M. Tercero-Jaime, M. T. Cutuli, and A. S. Olmeda . . . . . . 1396
Anopheles gambiae (Diptera: Culicidae) Susceptibility to Insecticides and Knockdown Resistance
Genes Prior to Introduction of Indoor Residual Spraying in 11 Districts in Ghana
D. B. Dery, S. Segbaya, V. Asoalla, F. Amoyaw, N. Amoako, A. Agyeman-Budu, A. Oduro,
S. Owusu-Agyei, and K. P. Asante . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1403

Knockdown Resistance Mutations in Aedes aegypti (Diptera: Culicidae) From Puerto Rico
Gustavo Ponce-Garcı́a, Samantha Del Rı́o-Galvan, Roberto Barrera, Karla Saavedra-Rodriguez,
Karina Villanueva-Segura, Gilberto Felix, Manuel Amador, and Adriana E. Flores. . . . . . . . . . . . . . . . . . . . . . . . . 1410

VECTOR/PATHOGEN/HOST INTERACTION, TRANSMISSION

Comparative Susceptibility of Ochlerotatus japonicus, Ochlerotatus triseriatus, Aedes albopictus,
and Aedes aegypti (Diptera: Culicidae) to La Crosse Virus
Jeffrey J. Bara, Allison T. Parker, and Ephantus J. Muturi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1415

Confirmation of Anopheles peditaeniatus and Anopheles sundaicus as Malaria Vectors (Diptera:

Culicidae) in Sungai Nyamuk Village, Sebatik Island North Kalimantan, Indonesia Using
an Enzyme-Linked Immunosorbent Assay
Sugiarto, Upik Kesumawati Hadi, Susi Soviana, and Lukman Hakim . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1422

Vector Competence of Anopheles kleini and Anopheles sinensis (Diptera: Culicidae) From the Republic
of Korea to Vivax Malaria-Infected Blood From Patients From Thailand
Ratawan Ubalee, Heung-Chul Kim, Anthony L. Schuster, Patrick W. McCardle, Siriporn Phasomkusolsil,
Ratree Takhampunya, Silas A. Davidson, Won-Ja Lee, and Terry A. Klein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1425

Fleas and Ticks in Carnivores From a Domestic–Wildlife Interface: Implications for Public Health
and Wildlife
Daniela A. Poo-Muñoz, Claudia Elizondo-Patrone, Luis E. Escobar, Francisca Astorga,
Sergio E. Bermúdez, Constanza Martı́nez-Valdebenito, Katia Abarca, and Gonzalo Medina-Vogel . . . . . . . . 1433

VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION

Anthropophily of Lutzomyia wellcomei (Diptera: Psychodidae) in an Atlantic Forest Conservation Unit
in Northeast Brazil
Marcos Paulo Gomes Pinheiro, José Hilário Tavares da Silva, Cássio Lázaro Silva Inacio, and
Maria de Fátima Freire de Melo Ximenes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1444
Evaluation of the Honey-Card Technique for Detection of Transmission of Arboviruses in Florida and
Comparison With Sentinel Chicken Seroconversion
Nathan D. Burkett-Cadena, Jennifer Gibson, Miranda Lauth, Tanise Stenn, Carolina Acevedo,
Rui-de Xue, James McNelly, Edward Northey, Hassan K. Hassan, Ali Fulcher, Andrea M. Bingham,
Jose van Olphen, Alberto van Olphen, and Thomas R. Unnasch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1449
Rickettsia (Rickettsiales: Rickettsiaceae) Vector Biodiversity in High Altitude Atlantic Forest Fragments
Within a Semiarid Climate: A New Endemic Area of Spotted-Fever in Brazil
Leonardo Moerbeck, Vinı́cius F. Vizzoni, Erik Machado-Ferreira, Robson C. Cavalcante,
Stefan V. Oliveira, Carlos A. G. Soares, Marinete Amorim, and Gilberto S. Gazêta . . . . . . . . . . . . . . . . . . . . . . . 1458

Synthesis and Functional Characterization of MAF-1A Peptide Derived From the Larvae of Housefly,
Musca domestica (Diptera: Muscidae)
Jing Zhou, Lina Kong, Nainai Fang, Bin Mao, and Hui Ai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1467

Bionomics and Vector Potential of Culex thriambus (Diptera: Culicidae) Mosquitoes in Lake County,
California
Brittany M. Nelms, Tara C. Thiemann, Danielle N. Bridges, Alan E. Williams, Michelle L. Koschik,
Bonnie M. Ryan, and Jamesina J. Scott . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1473

SHORT COMMUNICATION
Detection of Plasmodium falciparum Infection in Anopheles squamosus (Diptera: Culicidae) in an Area
Targeted for Malaria Elimination, Southern Zambia
Jennifer C. Stevenson, Limonty Simubali, Saidon Mbambara, Michael Musonda, Sydney Mweetwa,
Twig Mudenda, Julia C. Pringle, Christine M. Jones, and Douglas E. Norris . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1482

Sand Flies (Diptera: Psychodidae) From the Brazilian Atlantic Forest Domain Collected With Malaise
Traps
Paloma Helena Fernandes Shimabukuro, Jéssica Adalia Costa Moreira, and Tiago Silva da Costa . . . . . . . 1488

Development of a Rickettsia bellii-Specific TaqMan Assay Targeting the Citrate Synthase Gene
Joy A. Hecht, Michelle E. J. Allerdice, Felipe S. Krawczak, Marcelo B. Labruna, Christopher D. Paddock,
and Sandor E. Karpathy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1492

ERRATUM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1496

2016 Reviewers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1497

 Journal of Medical Entomology Advance Access published June 20, 2016

Journal of Medical Entomology, 2016, 1–3

doi: 10.1093/jme/tjw100
Research article
Vector/Pathogen/Host Interaction, Transmission

Confirmation of Anopheles peditaeniatus and Anopheles

sundaicus as Malaria Vectors (Diptera: Culicidae) in
Sungai Nyamuk Village, Sebatik Island North Kalimantan,
Indonesia Using an Enzyme-Linked Immunosorbent
Assay
Sugiarto,1 Upik Kesumawati Hadi,2,3 Susi Soviana,2 and Lukman Hakim4
1
Post Graduate Student of Doctoral Program, Department of Parasitology and Medical Entomology, Bogor Agricultural University,
Dramaga-Bogor, Jl Agatis Kampus IPB Darmaga, Bogor, 16680 (ugik.ok@gmail.com), 2Entomology Laboratory, Department of
Parasitology and Medical Entomology, Faculty of Veterinary Medicine – Bogor Agricultural University, Dramaga-Bogor, Jl Agatis

Downloaded from http://jme.oxfordjournals.org/ by guest on June 21, 2016

Kampus IPB Darmaga, Bogor, 16680 (upikke@gmail.com), 3Corresponding author, e-mail: upikke@gmail.com, and 4Master of
Public Health Program, Directorate of Post Graduate, University Sari Mutiara – Medan, Jl Kapten Muslim No.79, Medan - North
Sumatra

Received 29 March 2016; Accepted 21 May 2016

Abstract
Anopheles mosquitoes may be incriminated as malaria vectors by observing sporozoites in their salivary
glands and by testing heads or thoraces by an enzyme-linked immunosorbent assay (ELISA) to detect
Plasmodium species-specific circumsporozoite proteins (CSP). This study tested Anopheles collected in Sungai
Nyamuk Village for the presence of both Plasmodium falciparum and Plasmodium vivax CSP. The Anopheles
spp. were collected by human landing collection indoors and outdoors and by indoor and outdoor resting
catches in Sungai Nyamuk Village, Nunukan District, North Kalimantan Province from August 2010 to January
2012. Overall, 5,100 Anopheles spp. comprising 11 species were collected and 2,259 adult parous females were
tested by ELISA. Of these, only one Anopheles peditaeniatus Leicester (3.8%, n ¼ 26) and one Anopheles
sundaicus sensu lato (0.6%, n ¼ 157) that originated from outdoor biting catches tested positive for P. falciparum
CSP. The remaining females from indoor biting, outdoor resting, and indoor resting catches were negative for
P. falciparum and P. vivax proteins. Confirmation of these vectors biting outdoors indicated that P. falciparum
transmission may be occurring outside of houses by An. peditaeniatus and An. sundaicus.

Key words: An. peditaeniatus, An. sundaicus, outdoor biting, vector, ELISA

Malaria remains one of the serious health problems given priority
by the Ministry of Health (MoH) of Indonesia. In Indonesia, intense
transmission of malaria especially occurs in areas outside Java,
Madura, and Bali. The MoH (2015) reported that from the total
Indonesian population of 255,881,112 in 2015, 66,529,089 (26%
of population) lived in the malaria epidemic region.
Sebatik Island, Nunukan District, North Kalimantan Province
lies on the border of Indonesia and the eastern Malaysia state of
Sabah. Sebatik Island is one of the malaria endemic regions in
Nunukan District. The Health Agency of Nunukan District (2010)
reported that the annual malaria incidence (AMI) was 12.53 per
1,000 population in 2009, an increase from 10.58 per 1,000 popula-
tion in 2008. In 2013, 61 people in Sungai Nyamuk Village tested
positive for Plasmodium falciparum from a population of 7,525
people with an AMI of 8.11 per 1,000 population (MoH 2015).
Sebatik Island is an area of entrance for migrant workers from
Malaysia, and the control of malaria has become the main priority
in this region. Transmission of malaria on Sebatik Island has oc-
curred (indigenous), but the Anopheles vector[s] and place of trans-
mission (indoor vs outdoors) have not been established.
Our study determined the presence of Plasmodium circumsporo-
zoite proteins (CSP) in field-caught Anopheles females to incriminate
the vectors of malaria in Sungai Nyamuk Village. Positive results pro-
vided decision support for malaria vector control policies in its region.
Materials and Methods
Study Area
The topography of Nunukan Distric is dominated by coastal area
(altitude <25 m above sea level). Mosquitoes were collected from

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