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ARTIKELBPRATIWIenglish 3hal
ARTIKELBPRATIWIenglish 3hal
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Pratiwi Trisunuwati
Brawijaya University
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ABSTRACT
Cinnamomum burmannii was known as one of herbal medicine that has been used
traditionally as an ingredient of traditional medicine extract, contains cinnamaldehyde,
which is a naturally trigger of the body's immune response. This study aimed to evaluate the
immunostimulant effect of C. Burmannii and the increase of Granulocyte Receptor - 1 (GR-
1) that can be recognized from neutrophil expressing IFNγ and the increase of macrophage
phagocytosis activities.Thirty wistar mouse were appropriatelly infected with Salmonella
enteridis and then orally treated with C.burmanni alcohol extract at different dosages. The
mouse were observed for the increase of GR-1 expressing IFNγ using flow-cytometry and
the increase of macrophage phagocytosis activities using Giemza staining. The result showed
that increasing dosage of C. burmannii extract treatment increased the GR-1 level and the
IFNγ by 97.7% and macrophage phagocytosis activity by 98.1% (P<0.05). Thus, the
observations reflected clearly that C.burmannii ethanol extract can be utilized as
immunomodulator that increased immune response.
Table 1. Average number of CD4 in Salmonella enteritidis non-infected and infected and cinnamon (C.
burmanii) ethanol extract non-treated and treated animals
Number of CD4 (cells)
Treatment
Replic. 1 Replic. 2 Replic. 3 Replic. 4 Average + Sd
P0- 314.025 306.000 303.400 300.900 306.081,25 ± 5.690,46a
P0+ 1.587.425 1.551.000 1.428.875 1.514.750 1.520.512,50 ± 67.915,16b
P1+ 2.034.875 1.979.125 1.482.800 2.057.000 1.888.450,00 ± 272.411,00b
P2+ 2.554.375 2.593.500 2.672.325 2.013.725 2.458.481,25 ± 30.0534,49c
P3+ 3.243.275 2.662.375 3.329.100 3.185.925 3.105.168,75 ± 301.001,89d
P4+ 3.933.000 3.932.500 4.024.125 3.864.500 3.938.531,25 ± 65.507,98e
Notes: Values in the same column with different superscript showed significantly difference (P <0.05)
Figure 1. IFNγ as measured (flowcytometry) of mice treated C. Burmanii ethanol extract
showed significantly higher (p<0.05) than those in either the negative control
treatment or positive control treatment
Table 2. Average number of active macrophage cells that performed phagocytosis in Salmonella enteritidis
non-infected,infected and cinnamon (C. burmanii) treatment
Number of active macrophage cells
Treatment
Replic. 1 Replic. 2 Replic. 3 Replic. 4 Average + Sd
P0- 46 47 42 39 43.50±3.69a
P0+ 55 56 58 61 57.50±2.64b
P1+ 69 63 64 65 65.25±2.62c
P2+ 74 73 79 81 76.75±3.86d
P3+ 86 84 85 83 84.50±1.29e
P4+ 87 91 93 96 91.75±3.77f
Notes: Values in the same column with different superscript showed significantly difference (P <0.05)
CONCLUSION
C. burmannii extract treatment on S. characterized by the increase of GR-1
enteritidis infected M musculus BALB/c expressing IFNγ and the number of active
have potential as immunostimulatory as phagocyte macrophage cells.