This document describes how to perform the Tollens' test to identify reducing sugars. The test involves adding silver nitrate solution and sodium hydroxide to make Tollens' reagent. Then 2-5 drops each of sucrose, starch, lactose and glucose are added to separate test tubes along with 5 drops of Tollens' reagent and aqueous ammonia. A silver mirror forming in the tubes containing sucrose and glucose indicates a positive reducing sugar test.
This document describes how to perform the Tollens' test to identify reducing sugars. The test involves adding silver nitrate solution and sodium hydroxide to make Tollens' reagent. Then 2-5 drops each of sucrose, starch, lactose and glucose are added to separate test tubes along with 5 drops of Tollens' reagent and aqueous ammonia. A silver mirror forming in the tubes containing sucrose and glucose indicates a positive reducing sugar test.
This document describes how to perform the Tollens' test to identify reducing sugars. The test involves adding silver nitrate solution and sodium hydroxide to make Tollens' reagent. Then 2-5 drops each of sucrose, starch, lactose and glucose are added to separate test tubes along with 5 drops of Tollens' reagent and aqueous ammonia. A silver mirror forming in the tubes containing sucrose and glucose indicates a positive reducing sugar test.
1 mL of NaOH solution is added Mixed Tollens Reagent
Tollens Test
2-5 drops 2-5 drops 2-5 drops 2-5 drops
of sucrose of starch of lactose of glucose
Putted into each test tube
Added 5 drops of Tollens reagent Added aqueous ammonia solution drop by drop until all precipitate has dissolved Heated the mixture if no silver mirror is formed Formed silver mirror