Professional Documents
Culture Documents
Qualification HPTLC
Qualification HPTLC
By
Ankush Ramrao sule
M. Pharmacy 1st year
School of Pharmacy, Nanded
PRINCIPLE
Sample applicator
Developing chamber
Derivatization device
Immersion device
Plate heater
Scanning densitometer
Other accessories like:
(i) Plate coater
(ii) Drying rack
(iii) Plate cutter.
A. SAMPLE APPLICATOR
The samples are applied onto the layer as spots or
bands.
The samples are applied onto the layer as spots or
bands.
Usual concentration of applied samples 0.1 to 1 μg / μl
for qualitative Analysis and quantity may vary in
quantitation based on UV absorption 1 to 5 μl for spot
and 10 μL for band application.
Precision of the applied volume, exact positioning and
compactness of the application zones are important for
the quality of the analysis.
Three types of sample applicators are available:
1.Manual
2.Semiautomatic
3.Automatic.
B.DEVELOPING CHAMBER
Herbal fingerprinting
Herbal Analysis – Quantification
Pharmaceutical Science
Determination of purity of sample and
Identification of compounds
Identification of adulterants
Forensic science
Determination of mercury in water
Analysis of environmental pollution levels
Determination of ß-blockers like Metaprolol,
Alprenolol, Atenolol
OTHER ACCESSORIES
i. Plate coater
ii. Drying rack
iii. Plate cutter.
QUALIFICATIONS (CAMAG)