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Laboratory Manual Endn 832-1 Endodontics I Spring Quarter 2013
Laboratory Manual Endn 832-1 Endodontics I Spring Quarter 2013
SCHOOL OF DENTISTRY
DEPARTMENT OF ENDODONTICS
LABORATORY MANUAL
ENDN 832-1 ENDODONTICS I
SPRING QUARTER 2013
COURSE DIRECTORS
Bonnie Retamozo, DDS, MSD
INTRODUCTION:
The purpose of this technique manual is to aid the student in developing the skills which are
necessary to perform basic clinical endodontic procedures. Endodontic techniques are described,
instruments and their use are explained and the performance criteria for each laboratory
procedure are stated.
Successful completion of the pre-clinical technique course and the Endodontic Clinic orientation
will qualify you to treat patients in the Endodontic Clinic.
Successful endodontics requires attention to detail; this is true both in the pre-clinical laboratory
setting and in clinic.
COURSE GOAL: To provide the student with foundational knowledge and skills needed to
treat patients in the endodontic clinic.
7. Properly obturate an instrumented root canal system, including fitting of the master cone,
placement of the root canal sealer and lateral condensation of gutta percha.
PREREQUISITE:
A supply of extracted teeth is needed to complete some of the projects and the competency tests.
1
INSTRUMENT ISSUE / TOOTH SELECTION / RADIOLOGY
8. Recognize Gates-Glidden burs and state their use and describe how their size is
designated.
9. Recognize root canal files and explain their use, size and length designation and their
color coding system.
10. Recognize rubber stops and state their use and proper placement.
Endodontic explorer. One endodontic explorer is included in the issue (Fig. 1). It is a double-
ended instrument numbered DG16 and is used for locating coronal root canal orifices.
IT SHOULD NOT BE USED AS A SPREADER.
FIG. 1
2
Endodontic excavator. The issue has two double-ended long shank spoon excavators (Fig. 2),
numbered #31, #33L. They are longer than conventional excavators to allow curettage of the
pulp chamber. THESE INSTRUMENTS SHOULD NOT BE HEATED.
FIG. 2
Locking cotton pliers. The locking cotton pliers (Fig. 3) are designed specifically for
endodontics. They are to hold gutta percha points and paper points at fixed lengths; if needed
you may add a standard type of pliers from your regular issue.
FIG. 3
Endodontic ruler. One endodontic ruler (Fig. 4) located on your mirror handle is included in
the issue. It is graduated in 1 mm increments and is used for measuring endodontic files, gutta
percha points, paper points, etc.
FIG.4
3
File organizer. The file organizer (Fig. 5) is used to hold and organize intracanal instruments
(files) during root canal preparation.
FIG. 5
Burs and Gates Gliddens. The issue has a variety of long shank round burs for use in the slow
speed contra-angle handpiece. They are numbered 2, 4, 6, 8 and are designed for use in
completing access preparations. Also included are regular high speed burs (#2, 4, 6, 1558)
which are used in preparing the coronal access. Further, the issue has one vial of Gates Glidden
burs (Fig. 6). These are numbered 1 to 5 and are distinguished by bands on the shanks denoting
their numbers. These burs are designed to widen the coronal orifice of root canals and to aid in
developing a funnel shaped canal preparation.
FIG. 6
4
Intracanal hand instruments. The following “K-type” files are in the issue: #10 through #70 in
the 25 mm length. The files have colored handles which correspond to the file sizes (Table 1).
Files are used to clean and shape the root canal(s). Included also are rubber stops. These are
placed on the files (Fig. 7) to make the files conform to specific lengths needed. Files and rubber
stops may be autoclaved. Size of the file is determined by the formula X/100 = tip size in mm
(e.g. #20 file = 0.2 mm at 1 mm from the tip).
FIG. 7
TABLE 1
Instrument Color Instrument Color Instrument Color
Size Size Size
08 Gray 40 Black 90 White
10 Purple 45 White 100 Yellow
15 White 50 Yellow 110 Red
20 Yellow 55 Red 120 Blue
25 Red 60 Blue 130 Green
30 Blue 70 Green 140 Black
35 Green 80 Black
Film clip hangers. Six single clip film hangers are issued for use in the Preclinical Laboratory.
Always clip the film on the edge closest to the dimple. (Fig. 8)
FIG. 8
5
Irrigation. In the laboratory water is used to avoid contact with clothing and prevent accidental
spills. In the clinic, sodium hypochlorite (2.0%) is used for irrigation and extreme care must be
used to avoid contact with clothing due to its bleaching action.
Irrigation syringe. A Luer-Loc type, plastic 5 cc syringe with a specially designed needle is
used for irrigating the root canal system during cleaning and shaping. Fill the syringe, without
the needle attached, and squeeze out any water bubbles, attach the needle and you are ready to
irrigate the root canal.
Temporization Materials
Cavit. The tube of Cavit contains ready-mixed temporary filling material for the access cavity.
It can also be used for other purposes such as sealing leaky rubber dam margins around teeth and
other areas that need temporary seals against salivary contamination. This is an interim treatment
for teeth in the process of root canal therapy.
Woodson No. 2 instrument. The Woodson No. 2 instrument (Fig. 9) has a blade at one end and
a plugger at the opposite end. The blade is used to carry, place, and carve temporary filling
materials. The plugger is used for condensing filling materials into access preparations.
FIG. 9
6
Instruments for Obturation.
Endodontic spreaders. Two spreaders (Fig. 10) are issued. These are single-ended instruments
with tapered tips and identified as #D-11T and #D-11 S. These instruments are for lateral
condensation of the root canal filling material. CAUTION: DO NOT HEAT THESE
INSTRUMENTS
FIG. 10
Endodontic pluggers. Three flat ended pluggers (Fig. 11) identified as #1-3, 5-7, 9-11 are
issued. #1 is the smallest and #11 is the largest end. These are double-ended instruments used
for vertical condensation of endodontic filling materials. THEY MAY BE HEATED FOR
REMOVAL AND SOFTENING OF GUTTA PERCHA.
FIG. 11
7
Scissors. The pair of scissors (Fig. 12) issued are intended for cutting gutta percha points and
paper points to desired lengths. DO NOT USE for opening Cavit tubes and other heavy duty
chores.
FIG. 12
Root canal sealer. The type used in our laboratory and clinic is referred to as
Grossman’s/Roth’s sealer and is a zinc oxide and eugenol cement.
Gutta percha. Root canal fillings consist mainly of gutta percha, which is supplied in
two types: standardized ones for master cones (Fig. 13) and conventional for accessory
points (Fig. 14). More detailed information about gutta percha points will follow in
sections on obturation.
FIG. 13
8
FIG. 14
A. Prior to mounting the tooth should be cleaned of all adherent patient material.
B. Using a small ball of rope wax secure the crown of the tooth/teeth to a tongue depressor.
Also place a ball of wax at the apex of the tooth/teeth.
D. Insert tooth / teeth into the acrylic up to the CEJ of the tooth.
E. Once the acrylic has hardened the tooth model can be removed from the plastic cup and
stored in water to prevent drying of the tooth.
9
TOOTH SELECTION AND RADIOGRAPHIC TECHNIQUE FOR ALL PROJECTS
3. Learn to process analog radiographs using the portable chair side processing box.
MATERIALS:
1. Extracted teeth
2. X-Ray film
3. Film clip hangers
10
PROCEDURES:
11
3.1. Raise the light filter cover.
3.2. There are four containers inside (Fig. 19). The developing solution is always
placed in the container farthest to the left. Going from left to right, the next
container is water, followed by the fixing solution, and the last container to the
right is water. They are placed from left to right in the order they are going to be
used.
3.3. Place the exposed x-ray film and a film clip in the box.
3.6. Remove x-ray film from wrapper and place on film clip. Attach the film clip to
the edge of the x-ray film adjacent to the identifying dot (Fig. 20).
3.7. Immerse film in the developing solution (container #1) and slightly agitate several
times. Developing is complete when a definite visual image of the tooth appears
on the film (approximately 20 seconds).
12
3.8. Rinse film in water (container #2) for a few
seconds to remove the developing solution.
13
4.3. Roots and canals should have a curvature of
0-30º (Fig. 23). Excess curvature adds a
degree of difficulty not intended for this
course.
EVALUATION CRITERIA:
2. Quality of radiographs.
14
PROJECT 1: ACCESS PREPARATION
1 Plastic Anterior Tooth
1 Maxillary Incisor - Mounted
1 Mandibular Incisor - Mounted
1 Canine - Mounted
FIG. 24
17
ACCESS PREPARATION MAXILLARY CENTRAL OR
LATERAL INCISOR
PROCEDURES:
18
9. After locating the canal orifice, the lingual shoulder
should be removed by carefully inserting a #2 long shank
slow speed bur into the canal and with pressure to the
lingual, work the bur from within the canal to the outside
(Fig. 30).
10. The walls of the access cavity may be flared and made
smooth by using a diamond finger bur or a #1558 bur.
REMEMBER, the flare is from the inside to the
outside like an inlay preparation (Fig. 31).
12. Irrigate and remove all debris from the pulp chamber,
locate the canal orifice with the endodontic explorer,
check the walls of the access cavity for smoothness
and lack of overhangs, particularly in the pulp horn
areas. Use the evaluation criteria to evaluate your
work before asking the instructor to evaluate it.
EVALUATION CRITERIA
19
20
ACCESS PREPARATION: MANDIBULAR CENTRAL OR
LATERAL INCISOR AND CANINES
PROCEDURES:
21
9. The lingual constriction should be removed by carefully
inserting a No. 2 slow speed round bur into the canal.
While applying lingual pressure to the bur, work it from
the inside to the outside (Fig. 38). This will accomplish
unobstructed access to the apical area of the root canal.
EVALUATION CRITERIA
22
ACCESS PREPARATION: MAXILLARY AND
MANDIBULAR PREMOLARS
PROCEDURES:
23
6. After the preliminary access has been
completed, use the same No.2 round bur to
penetrate through the dentin into the pulp
chamber. The penetration point should be in the
center of the access outline (Fig. 45).
EVALUATION CRITERIA
11.1. Proper location of access
11.2. Correct outline form
11.3. Smooth walls with proper flare
11.4. Canal orifice(s) located
11.5. Access cavity free of debris
24
ACCESS PREPARATION: MAXILLARY AND
MANDIBULAR MOLARS
PROCEDURES:
25
11.3 Smooth walls with
proper flare
5. Contact the instructor for evaluation of the 11.4 Canal orifices
preliminary access. located
11.5 Access cavity free of
6. After the preliminary access has been debris
completed, use the same No. 2 round bur to
penetrate through the dentin into the pulp
chamber. The penetration point should be in the
center of the access outline (Fig. 55).
EVALUATION CRITERIA
11.1 Proper location of access
11.2 Correct outline form
26
PROJECT 3: ROOT CANAL PREPARATION
3 Canals – Clean and Shape
27
PROCEDURES: .
After the proper coronal access has been achieved, the next
step in endodontic therapy is to clean the root canal and to
properly shape it to receive the filling material.
3. Place the #10 file into the canal until the tip of the file
contacts the end of the root canal system.
28
6. Next you need to establish the working length. This is
done by subtracting 1 mm from the total length.
Document the working length in your portfolio
under the correct tooth.
13. Set the rubber stops on files #15, 20, 25, 30 and 35 at
the working length.
29
14. With the root canal filled with sodium hypochlorite,
insert the file into the root canal until the rubber stop
contacts the occlusal/incisal reference point using a
slight clockwise rotation and a push-pull stroke with a
circumferential motion. Begin with the #15 file and
work up in sequence to the #30 or #35 file depending
on the size of the canal.
15. Now, set the rubber stop on file #40 ½mm shorter than
the working length, #45 1mm shorter, #50 1.5mm
shorter, etc., up through file #70 (Fig. 68). Using the
same filing motion as above, insert the files in a
sequential order, into the root canal until the rubber stop
contacts the occlusal reference point.
REMEMBER:
EVALUATION CRITERIA
30
COMPETENCY TEST I:
ACCESS: Mounted:
1 Maxillary Anterior
1 Mandibular Anterior
1 Posterior Multi-Canal molar of student’s choice.
31
3. The teeth should be mounted in acrylic:
32
EXAMINATION PROCEDURE
3. Access:
4. General performance:
33
PROJECT 4: OBTURATION - LATERAL
CONDENSATION
Obturate 3 prepared canals
After proper cleaning and shaping of the root canal system, the
next step in endodontic therapy is the obturation of the root
canal(s).
34
PROCEDURES:
36
37
8. Contact the instructor to evaluate the spreader selection and fit.
9.1. If you use the #D11 spreader, you should use the size medium-fine accessory
points.
10.1. Place the root canal sealer and liquid on the small glass slab (Fig. 88).
NOTE: The root canal sealer powder and liquid are provided from the laboratory
supply cart. The assistant will pass it out to you.
10.2. Spatulate small portions of the powder into the liquid using a wide circular stroke
until a smooth homogenous thick creamy consistency is obtained
10.3. The proper consistency has been achieved when the spatula is slowly raised one
inch from the mass of sealer and the “string” of sealer holds (“strings outs”) for 4 to
5 seconds before breaking. (Fig. 89).
11. Contact the instructor to evaluate your selection of accessory gutta percha points and
your sealer mix.
38
12. Coating the root canal walls with sealer.
12.1. Place a rubber stop on the next to largest working length file and set it at the
working length.
12.2. Coat the apical ½ of the file with sealer (Fig. 90).
12.3. Insert the file into the root canal to the working length.
12.4. Slowly rotate the file counter clockwise while at the same time withdrawing
the file from the canal. To ensure that the canal walls are entirely coated with
sealer, it may be necessary to repeat this procedure two or three times.
13. Placing the master cone into the prepared root canal.
13.1. Hold the master cone with the locking cotton pliers at the previously
determined working length.
13.2. Coat the apical ½ of the master cone with sealer (Fig. 91).
13.3. Slowly insert the master cone into the canal until it is completely seated to
place (Fig. 92).
39
14. Lateral condensation of gutta percha
14.1. The extruding portion of the master cone usually fits eccentrically in the canal,
leaving one side with more space. Lateral condensation can best be achieved
in this space along side of the master cone (Fig. 93).
14.2. Insert the spreader in the chosen side between the master cone and the wall of
the root canal and exert apical pressure until resistance is met.
14.3. Do not penetrate beyond the length indicated by the rubber stop (Fig. 94).
14.4. Rotate the spreader handle 180º several times to disengage the spreader point
from the gutta percha and slowly remove the spreader from the canal.
14.5. Repeat the spreader insertion, rotation and withdrawal three or four times, always
reinserting the spreader in the same position. This will condense the gutta percha
laterally.
14.6. Coat the apical 2-3 mm of an accessory cone with sealer and insert it into the
space created by the spreader (Fig. 91 and Fig. 96).
14.7. Reinsert the spreader in the same position, between the canal wall and the gutta
percha, and continue the lateral condensation process adding accessory cones
until the root canal space is completely obturated (Fig. 97 and Fig. 98).
Fig. 93
Fig. 94 Fig. 96
Fig. 97 Fig. 98
40
15. Contact the instructor to evaluate the lateral condensation.
16.1. Heat one end of the #9-11 gutta percha plugger in a Bunsen burner flame.
16.2. Remove the excess gutta percha 1 mm below the facial CEJ.
16.3. With the cold end of the gutta percha plugger, vertically condense the gutta
percha in the root canal space by applying firm apical pressure with the plugger
(Fig. 100).
41
Remove the excess sealer from the access with a cotton pellet saturated with alcohol (Fig. 101).
16.4. Take a radiograph of the finished obturation to evaluate the density of the gutta
percha filling.
17. Have your laboratory instructor evaluate your performance using the Endodontic
Laboratory Evaluation form provided for this project.
EVALUATION CRITERIA
Fig. 102
Fig. 101
42
PROJECT 5: ROOT CANAL THERAPY
1 Anterior Tooth
1. Identify the instruments used for all technical phases of endodontic treatment.
The student will set up and have available the following instruments and materials prior to
starting the project.
43
PROCEDURES:
1. Study the radiographs of the project canal(s) to determine the size, shape and coronal
extension of the pulp chamber.
2. Contact the instructor for evaluation of the completed access cavity preparation.
Using the endodontic ruler, measure the length of the image of the tooth on the
radiograph from the occlusal reference point to the radiographic root apex.
4.1. Position the rubber stop on the #10 SS file 1-2mm shorter than the estimated
length.
4.3. Place water in the pulp chamber using the irrigating syringe.
4.4. Place the #10 file into the canal orifice and slowly. Advance it apically with a
slight back-and-forth or “watch winding” motion until the rubber stop touches the
reference point.
4.5. If you encounter resistance to the apical movement of the file, DO NOT FORCE
IT TOWARD THE APEX. Instead, pull the file back 1-2 mm, rotate the file
slightly while slowly and gently advancing it toward the apex again. By carefully
approaching, retreating and approaching - all the while rotating the file back-and-
forth, you will be able to bypass calcific deposits in the pulp tissue and negotiate
canal curvatures (Fig. 104).
4.6. Now examine the root apex. If the file has not exited through the apical foramen,
continue to advance it until it is visible (Fig. 105).
5.1. Position the file so that the tip is flush with the root surface. Adjust the rubber
stop so that it contacts the coronal reference point you have chosen.
44
5.2. Remove the file from the canal. If it is still curved, straighten it and measure the
distance from the rubber stop to the file tip. This measurement will be the total
root canal length or patency length.
5.3. Adjust the rubber stops on the #15-#35 files so that they are set at the working
length. Place them in the file organizer so that they are convenient and ready to
use (Fig. 106).
5.4. Place sodium hypochlorite into the pulp chamber using the irrigating syringe.
Starting with the #15 file, insert the file into the canal and slowly advance it
apically until the rubber stop reaches the coronal reference point.
5.5. Take a “working length” radiograph with the #15 file in place, position the tooth
on film and stabilize it with a small piece of soft wax. Expose and process
the film. The radiograph should demonstrate the file 1mm short of the total root
length (Fig. 107).
6.1. Follow the principles of the “Passive step-back technique” (Project #2)
6.2. After you have completed the root canal preparation, place the largest working
length file in the canal to the working length and expose a radiograph. Compare
the position of the tip of the file on this radiograph with the one taken with the
#15 file in place. They should be in the same place relative to the total root canal
length.
45
EVALUATION CRITERIA
The student’s performance will be evaluated for: (See project evaluation form)
46
PROJECT 7: COMPLETE ROOT CANAL
TREATMENT OF A PLASTIC
MAXILLARY MOLAR #14
47
PROCEDURES:
6.4. Place the #10 file into the canal orifice and slowly
advance it apically with a slight rotary back-and-
forth / “watch winding” motion until the rubber stop
touches the reference point (Fig. 119).
48
6.5. If you
encounter
resistance
to the
apical
movement
of the file,
DO NOT
FORCE
IT
TOWAR
D THE
APEX.
Instead,
pull the
file back
1-2 mm,
rotate the
file
slightly
while
slowly and
gently
advancing
it toward
the apex
again. By
carefully
approachin
g,
retreating
and
approachin
g, all the
while
rotating
the file
back and
forth, you
will be
able to
bypass
calcific
deposits in
the pulp
49
tissue and negotiate canal curvatures (Fig. 120).
6.6. Now examine the root apex. If the file has not
exited through the apical foramen, continue to
advance it until it is visible (Fig. 121).
7.1. Position the file so that the tip is flush with the root
surface at the apical foramen.
7.3. Repeat this procedure for all canals and record the
appropriate data in your portfolio.
Take working x-rays with the largest working length files placed to
the working length. Repeat the same schedule of x-rays that you
took to verify the working length earlier in this project (see
procedure #8).Contact the instructor to evaluate the radicular
preparation.
EVALUATION CRITERIA
1. Access Preparation
2. Length Determination
3. Radicular Preparation (Cleaning and Shaping)
4. Radiographs Properly Exposed and Processed
5. Radiographs Properly Mounted
51
[TBA]
GOAL: To evaluate your ability to apply the principles of endodontic access cavity
preparation, radicular cavity preparation and obturation using lateral condensation of
gutta percha.
1. TOOTH SELECTION: Any multi-canaled posterior tooth may be used except a tooth
with the crown not intact. Intact means that the crown cannot display any fractures that
obviously involve the pulp chamber. Craze lines, restorations which do not cover the
entire lingual or occlusal surface, and enamel fractures are acceptable. Teeth with
existing restorations are acceptable as long as the restoration is intact with good margins.
Teeth with caries are not acceptable.
B. Place a ball of soft pink base - plate wax at the apex of the roots. The wax ball
should measure 4 mm or more and must be clearly visible on the radiograph after
the tooth has been mounted in acrylic.
C. Cut a small horizontal notch in the coronal third of the tooth root on the facial or
lingual surface with a bur. This notch will lock the tooth into the acrylic and
prevent removal or displacement of the tooth. Be careful not to enter the root
canal space.
D. Place the tooth in the model, centering it in the acrylic and embedding it to just
below the cemento-enamel junction. Make sure that the wax at the root apex is
not touching the bottom of the mold.
E. After the acrylic has hardened, remove from plastic cup. The tooth root and ball
of wax must be completely covered in the solid acrylic block
G. Expose and develop radiographs of the mounted tooth from a straight on and from
a mesial or distal direction. The developed radiographs must clearly show the
radiolucency (minimum 4 mm) of the wax at the root apex. The pulp chamber
and root canal should be clearly visible on the radiograph.
H. Store the mounted tooth in liquid until the time of the competency test.
EXAMINATION PROCEDURE
1. Before you ask your instructor to evaluate the examination, be sure that you perform a
self-evaluation by checking the evaluation criteria for that section to make sure that you
have done everything required.
2. Pretreatment check: Ask your instructor for a start check. They will evaluate the tooth
selection and mounting section at the same time.
3.1. Access the tooth appropriately (should look like a text book/DVD access).
53
3.3. The radiograph for length determination should be taken from the facial-lingual
direction.
4. Instrumentation
4.1. Proceed with cleaning and shaping of the root canals using the technique and files
of your choice.
4.2. When you have completed the canal preparation, take a radiograph with the
largest working length file in place to the working length from the facial-lingual
direction.
5. Master cone selection and fit, spreader selection and fit, accessory cone selection and
sealer mix:
5.2. Take a radiograph with the master cones in place from the facial-lingual direction.
5.4. Select and have the proper accessory cones ready for use.
6. Obturation:
6.1. Proceed with obturation of the root canal spaces with gutta percha and sealer
using lateral condensation.
6.2. The gutta percha should be removed internally from the pulp chamber to the level
of the facial-lingual cervical line.
6.4. Take two radiographs of the finished obturation, one from the facial-lingual and
the other from the mesial-distal direction.
7. General performance:
7.1. You will be evaluated during the examination for the availability of necessary
instruments and supplies, the neatness and organization of your work area and
your adherence to the proper laboratory dress code.
54
7.2. The radiographs for this examination must be properly mounted in the mount
provided (see example below) and labeled with your box number and the project
number.
7.3. Do not place wet radiographs in the plastic mount. The radiographs should be
thoroughly dried before mounting.
8. Turn in the mounted tooth and the evaluation forms for grading in the boxes
provided at the supply table.
55