Hitesh Gohel et al., (2013) Int. J. Res. Phytochem. Pharmacol.

, 3(3), 142-148

ISSN: 2231-010X
www.ijrpp.pharmascope.org Research Article

A detail physicochemical investigation of a polyherbal formulation - Cutisora

tablet
1 1 2* 2 2 2
Hitesh Gohel , Bhatt J.J , Vidhi Parikh , Hardik Soni , Prateek Patel , Ghanshyam Patel
1
Department of Chemistry, Krantiguru Syamji Krushna Verma Kachchha University ,Bhuj, Gujrat, India
2
Vasu Research Centre (A Division of Vasu Healthcare Pvt. Ltd.), 896/A, G.I.D.C., Makarpura, Vadodara, Gujarat,
India

ABSTRACT

In order to have a good coordination between quality of raw materials, in process materials and the final prod-
ucts, it is essential to have reliable, specific and sensitive quality control methods. The quality of herbal drugs is
the sum of all factors, which contribute directly or indirectly to the safety, efficacy and acceptability of the prod-
uct. Standardization of drugs means confirmation of its identity and determination of its quality and purity. Cutiso-
ra tablet, a polyherbal formulation is used for the treatment of psoriasis. Raw materials as well as finished product
were standardized using standard parameters such as organoleptic parameters, physico-chemical parameters,
Comparative High Performance Thin Layer Chromatographic study (HPTLC), Microbial and Heavy metal analysis.
The investigation data revealed the status of standardization at the level of raw materials and finished product.
The obtained results were used to establish overall quality of the final product.
Keywords: Polyherbal formulation; Cutisora Tablet; Standardization; HPTLC study
INTRODUCTION analytical methods, which can reliably profile the phy-
tochemical composition, including quantitative analys-
Ancient Indian literature comprises a remarkably broad
es of marker/ bioactive compounds and other major
definition of medicinal plants and considers all plant
constituents, is a major challenge to scientists. Stan-
parts to be potential source of medicinal substances.
dardization minimizes batch-to-batch variation; assures
However, key obstacle, which has hindered the accep-
safety, efficacy, quality and acceptability of the poly-
tance of the alternative medicines in the developed
herbal formulations (Ahmad et al, 2006).
countries, is the lack of documentation and standardi-
zation. There is a real need for documentation on re- The present study was carried out with specific aim to
searches, which have been so far carried out on tradi- establish standards for Cutisora tablet. Cutisora tablet
tional medicines. With this backdrop, it become ex- contains following ingredients which having known
tremely important to make an effort towards standar- effect against skin disorders like psoriasis.
dization of plant based medicines.
Wrightia tinctoria extract has Anti-inflammatory activi-
Standardization is a system that ensures a predefined ty, Anti-fungal activity (Elumalai et al) Tinospora cordi-
amount of quantity, quality & therapeutic effect of folia anti inflammatory, analgesic and immunosuppres-
ingredients in each dose (Zafar et al, 2005). No medi- sive activity (Anonymous, 1998). Azadirachta Indica is
cine including Herbal product can be considered scien- main ingredient of poly herbal formulation &it is useful
tifically valid if the drug tested has not been authenti- in all skin conditions especially in burning sensation
cated and characterized in order to ensure reproduci- (Anonymous, 2000). Adhatoda vasica bacterial antisep-
bility in the manufacturing of the product. Moreover, tic and it is used in various skin infections (Anonymous,
many dangerous and lethal side effects have recently 2004). Trichosanthes dioica is effective in skin disease
been reported, including direct toxic effects, allergic including Pruritus (Anonymous, 2000). Solanum xan-
reactions, effects from contaminants, and interactions thocarpum has antifungal activity (Anonymous, 1998).
with herbal drugs (Vaidya et al, 2007). Therapeutic ac- Plumbago zeylanica has anti inflammatory and anti
tivity of an herbal formulation depends on its phyto- microbial activity (Anonymous, 2008). Acacia catechu
chemical constituents. The development of authentic has characteristic of Kushthaghna and Kandughna. So it
is used in all skin disease and intense itching (Anonym-
* Corresponding Author ous, 2000). Curcuma longa remarkable anti inflamma-
Email: vidhi@vasuresearch.com tory activity .It also has anti ulcer and anti bacterial
Contact: +91-9227788565, 0265 2657701/02 property (Anonymous, 1998). Kaishor Guggulu powder
Received on: 22-04-2013 formulation made using more than 10 different ingre-
Revised on: 17-08-2013 dients which are useful in treatment of psoriasis (Ano-
Accepted on: 18-08-2013 nymous, 2004).

142 ©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology
 Hitesh Gohel et al., (2013) Int. J. Res. Phytochem. Pharmacol., 3(3), 142-148

MATERIAL & METHOD mentioned in the book Standardization of botanicals,

Dr. V. Rajpal, 2005.
Polyhedral tablet formulation containing extracts and
powder of various plant constituents i.e. Adhatoda HPTLC analysis of Raw material with finished product
vasica Nees, Solanum xanthocarpum Schard & Wendl,
Sample preparation of Raw materials:
Tinospora cordifolia Willd, Trichosanthes dioica Roxb,
Azadirachta indica A Juss, Curcuma longa Linn, Acacia Extract 1.0g of Extract / Powder (Reference Standard /
catechu (Linn) Willd, Plumbago zeylanica Linn, Wrigh- Test Drug) with 10 mL of Methanol with constant shak-
0
tia tinctoria R.Br, and Kaishor guggulu respectively. ing for 05 minutes. Heat on water bath at 90-100 C for
5 minutes, Filter it through whatman filter paper no.41.
Chromatographic Conditions
Take the filtrate for TLC/HPTLC Profiling.
Application Mode CAMAG Linomat 5-Applicator
Sample preparation of Cutisora Tablet (Finished prod-
Filtering System Whatman filter paper No.41
uct):
MERCK - TLC / HPTLC
Stationary Phase Silica gel 60 F254 on Aluminum Extract 3.0g powder of Cutisora Tablet with 10 mL of
sheet methanol with constant shaking for 05 minutes. Heat
0
Application (Y axis) on water bath at 90-100 C for 5 minutes, Filter it
10mm
Start Position through whatman filter paper no.41. Take the filtrate
Development (Y- for TLC/HPTLC Profiling.
axis) 90mm from plate base
Comparative HPTLC study of TLC plates was carried out
End Position
at 3 different wavelengths 254 nm, 366 nm and 540
Band length 8 mm
nm respectively. It was justified on the basis of best
Development CAMAG TLC Twin Trough
visualization of TLC plate at specific wavelength.
Mode Chamber
Chamber Satura- Table 1: Mobile phase/ solvent systems
tion Time 30 minutes
Ingredient Solvent System
@254nm, @366nm, @ Visible Toluene: Acetone: Methanol
Wrightia tinctoria
Visualization (after spray of Anisaldehyde (8.9:0.9:0.2)
Sulphuric acid reagent) Tinospora cordifolia Chloroform: Methanol (9:1)
Derivatization CAMAG – Dip tank for about 1 Toluene: Ethyl acetate: Formic
mode minute Azadirachta indica
acid (10:3:1)
TLC Plate Heater Toluene: E.A.: Methanol: am-
Drying Mode, 0 Adhatoda vasica
Preheated at 100± 5 C for 3 monia (8:2:0.2)
Temp. & time
minutes Trichosanthes dio- Toluene: E.A.: Formic acid
Evaluation of Quality Control Parameters for Raw Ma- ica (10:3:1)
terial Solanum xantho- Toluene: E.A.: Diethyl amine
carpum (7:2:1)
Organoleptic Parameters: Organoleptic parameters Toluene: E.A.: F.A.: Methanol
like colour, odour and taste of all the extracts were Plumbago zeylanica
(3:3:0.8:0.2)
carried out. These parameters helped in visual identity Toluene: E.A.: Formic acid
of drug extract which were derived from plant by vari- Acacia catechu
(10:3:1)
ous extraction procedures. Chloroform: Methanol: G.A.A
Curcuma longa
Physicochemical parameters: Raw materials and Cuti- (9.5:0.5:0.1)
sora Tablet were evaluated for total ash value, acid Pet. Ether: E.A.: Methanol
Kaishor Guggulu
insoluble ash, moisture, water soluble extractive value, (6:2:0.5)
pH (1% w/v) according to methods described in the
Heavy Metal Analysis: Heavy metal analysis was per-
Ayurvedic Pharmacopoeia of India.
formed using Shimadzu AA-6300, HVG and digestion
Cutisora Tablet (finished product) was also evaluated was done on CEM MARS Express microwave digestive
for different other physicochemical parameters viz. system. Sample amount 0.5g and 8ml 69% Nitric acid
Moisture, pH, Weight variation and Disintegration time were taken in the Teflon PFA 75ml vessels. Parameters
as per the methods mentioned in Indian Pharmaco- used for the digestion were Power 400 W with 100%,
O
poeia. Hardness was tested using hardness tester Ramp 20 minutes to attempt temperature 150 C and
where as Thickness and Diameter were measured using hold for 10 minutes. After completing digestion
Vernier caliper. process, the sample was diluted up to 50ml by distilled
water and filter through Whatman filter paper No. 1.
Quantitative estimation of different phytoconstituents
The standards of Lead (Pb), cadmium (Cd), arsenic (As)
such as Alkaloid, Bitter, Tannin and Curcuminoids in
and mercury (Hg) were prepared and the calibration
respective extracts was performed as per the method
curve was developed for each of them. Samples were
©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology 143
 Hitesh Gohel et al., (2013) Int. J. Res. Phytochem. Pharmacol., 3(3), 142-148

analyzed by using these standard curves. The permissi- Staphylococcus aureus (ATCC 6358) were obtained
ble limit for Heavy metal in herbal drugs is consider as from NCIM Pune. The media used for the microbial
per describe limit given by Department of Ayush. limit test were of HiMedia Pvt. Ltd.
Table 2: Organoleptic character of all ingredients of Comparative Organoleptic characters of the extracts
Cutisora Tablet (Raw materials) and powder used in tablet formulation are as tabulated
in Table 2.
Name of In- Description
gredient Colour Odour Taste Physicochemical parameters such as moisture (by
Wrightia tinc- LOD), Water soluble extractive value, Total ash, Acid
Brown Characteristic Astringent
toria insoluble ash, Water soluble ash, and pH were deter-
Tinospora Light mined in raw materials. These values are mentioned in
Characteristic Bitter
cordifolia Brown Table No. 3, which shows that most of the raw mate-
Azadirachta Greenish rials do not have considerable amount of acid insoluble
Characteristic Bitter
indica brown
ash and water soluble ash. Other than physicochemical
Adhatoda vasi-
Brown Characteristic Bitter parameters, average weight, hardness, thickness, di-
ca
ameter and disintegration time in 3 different batches
Trichosanthes Dark
Characteristic Bitter of Cutisora Tablet were carried out. The results are as
dioica Brown
Solanum Dark tabulated in Table No. 4
Characteristic Bitter
xanthocarpum Brown Phytochemical constituents like alkaloid and bitter by
Plumbago
Brown Characteristic Characteristic gravimetric method, tannin by titrimetric method and
zeylanica
curcuminoids by spectrometry were determined in raw
Acacia catechu Brown Characteristic Astringent
Curcuma longa Yellow Aromatic Bitter
materials and Cutisora Tablet (Table No. 5).
Kaishor Dark HPTLC analysis
Characteristic Characteristic
Guggulu brown
In HPTLC analysis, the sample shows comparison of
Microbial Limit Test: Microbial analysis was carried individual extract & Powder with finished product. The
out for all the extracts as per procedure of Indian visualization of TLC plates was carried out in all 3 dif-
pharmacopoeia 2007. It included Total bacterial count, ferent wavelengths ie, 254nm, 366nm and 540 nm.
Total Fungal Count, Presence of Escherichia coli, Sal- From this only the best visualization result was se-
monella spp, Pseudomonas aeruginosa, and Staphylo- lected and included in our study along with its 3D im-
coccus aureus. Pure culture of Escherichia coli (NCIM: age. The Rf value thus found during this study indicates
2065; ATCC: 8739), Salmonella abony (NCIM: 2257 the prominent presences of raw material in the fi-
NCTC: 6017), Pseudomonas aeruginosa (ATCC 9027),
Table 3: Physicochemical Parameters for Cutisora Tablet (Raw materials)

Physicochemical Parameters
Sr. Name of ingre-
No dient M/C by LOD
WSE (%) pH TA (%) AIA (%) WSA (%)
(%)
1. W.tinctoria 96.24±0.05 4.62±0.04 3.32 ± 0.06 6.53±0.03 1.86±0.04 3.58±0.02
2. T. cordifolia 76.48±0.52 5.70±0.02 1.91 ± 0.05 9.00±0.05 1.52±0.06 3.89±0.03
3. A. indica 97.52±0.44 5.35±0.01 2.29 ± 0.02 6.65±0.01 2.59±0.01 3.86±0.04
4. A. vasica 92.80±0.66 7.03±0.02 3.62 ± 0.03 11.30±0.06 4.12±0.07 5.28±0.02
5. T. dioica 95.20±0.10 6.19±0.04 3.36±0.07 9.98± 0.04 3.68±0.08 4.67±0.05
6. S.xanthocarpum 88.72±0.26 5.31±0.05 1.06 ± 0.06 8.94± 0.03 4.26±0.01 5.60±0.06
7. P. zeylanica 94.32±0.76 4.57±0.05 2.89± 0.09 4.59± 0.02 2.18±0.09 1.94±0.05
8. A. catechu 96.88±0.05 4.36±0.04 2.43 ± 0.01 6.48± 0.07 2.59±0.04 3.95±0.02
9. C. longa 21.52±0.028 4.08±0.01 2.91 ± 0.08 1.99± 0.05 0.62±0.09 0.83±0.07
10. Kaishor Guggulu 35.12±1.026 2.46±0.03 4.78 ± 0.01 9.80± 0.01 4.27±0.01 5.28±0.06
3.54±0.05(LOD)
11. Cutisora Tablet 64.08±0.08 5.38±0.05 16.62±0.07 5.07±0.06 5.97±0.04
1.09 ±0.06 (KF)
* Results indicated in Mean + SEM, where n=3.
WSE – Water soluble extractive, M/C – Moisture content, LOD – Loss on drying, KF – Karl Fischer, TA –
Total Ash, AIA – Acid insoluble ash, WSA - Water soluble ash
W.tinctoria - Wrightia tinctoria, T. cordifolia - Tinospora cordifolia, A. indica - Azadirachta indica, A. vasica
- Adhatoda vasica, T. dioica - Trichosanthes dioica, S.xanthocarpum- Solanum xanthocarpum, P. zeylanica
- Plumbago zeylanica, A. catechu - Acacia catechu, C. longa - Curcuma longa
144 ©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology
 Hitesh Gohel et al., (2013) Int. J. Res. Phytochem. Pharmacol., 3(3), 142-148

nished product which is used to establish its quantita-

tive presence.
Table 4: Quality control parameters of Cutisora Tablet
(finished product)

Parameters Result
Batch
Batch 1 Batch 2
3
Colour Purple Purple Purple
Shape Round Round Round
Film Film
Coating Film coated
coated coated
Standardization Parameters Figure 1: A) HPTLC finger printing of extract of Wrigh-
Average tia tinctoria. Track 1and 2: 8 μg/ml of Methanol extract
644.51±0.08mg
weight
of leaves of extract W.tinctoria Track 3 and 4: 8 μg/ml
Diameter 12.90±0.06
of Methanol extract of Cutisora tablet. Under UV 366
Thickness 5.11±0.04
Hardness 3.5 kg/cm
2 nm raw materials showed specific Rf values 0.25, 0.63
Disintegration and 0.80. From that Rf value 0.63 showed prominent
48 min 19 sec
time presences in finished product. B) HPTLC finger printing
of extract of Tinospora cordifolia (UV 366 nm). Track
* Results indicated in Mean + SEM, where n=3.
1and 2: 8 μg/ml of Methanol extract of leaves of ex-
Table 5: Quantitative determination of Actives tract Tinospora cordifolia. Track 3 and 4: 8 μg/ml of
Name
Methanol extract of Cutisora tablet. Under UV 366 nm
Alkaloid Bitter Tannin Curcuminoids
of
(%) (%) (%) (%)
raw materials showed specific Rf values 0.06, 0.11,
Ingredients
0.16, 0.25 and 0.30. From that Rf values 0.06 and 0.11
1.14
W.tinctoria NA NA NA showed prominent presences in finished product.
±0.06
2.98
T. cordifolia NA NA NA
±0.04
3.70
A. indica NA NA NA
±0.03
0.61
A. vasica NA NA NA
±0.09
1.96
T. dioica NA NA NA
±0.02
2.52
S.xanthocarpum NA NA NA
±0.05
0.16
P. zeylanica NA NA NA
±0.01
23.69
A. catechu NA NA NA
±0.04
7.20
C. longa NA NA NA
±0.08
5.02 9.40 Figure 2: A) HPTLC finger printing of extract of Azadi-
Kaishor Guggulu NA NA
±0.01 ±0.07
0.74 3.44 7.09 0.39 rachta indica. Track 1 and 2: 8 μg/ml of Methanol ex-
Cutisora Tablet
±0.06 ±0.04 ±0.07 ±0.14 tract of leaves of extract A.indica. Track 3 and 4: 8
* Results indicated in Mean + SEM, where n=3. NA: Not μg/ml of Methanol extract of Cutisora tablet. Under UV
applicable 254 nm raw materials showed specific Rf values0.60
and 0.74. These both Rf values showed prominent pre-
Discussion
sences in finished product. B) HPTLC finger printing of
All ingredients used in Cutisora Tablet are standardized extract of Adhatoda vasica. Track 1and 2: 8 μg/ml of
on the basis of organoleptic parameters, physico-
Methanol extract of leaves of extract A.vasica. Track 3
chemical parameters and different assays of their ac-
tives including HPTLC analysis. Moisture content/LOD and 4: 8 μg/ml of Methanol extract of Cutisora tablet.
value of all ingredient and Cutisora tablet were found Under UV 254 nm raw materials showed specific Rf
in their minimum range that indicates ingredients were values 0.09, 0.18, 0.28 and 0.80. From that Rf values
of good quality and thus will not deteriorate. Equally 0.28 and 0.80 showed prominent presences in finished
important in the evaluation of crude drugs, is the ash product.
value and acid insoluble ash value determination. The

©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology 145
 Hitesh Gohel et al., (2013) Int. J. Res. Phytochem. Pharmacol., 3(3), 142-148
Figure 3: A) HPTLC finger printing of extract of Tricho-
santhes dioica. Track 1 and 2: 8 μg/ml of Methanol
extract of Panchang of extract T.dioica. Track 3 and 4: 8
μg/ml of Methanol extract of Cutisora tablet. Under UV
366 nm raw materials showed fluorescent spot at spe-
cific Rf values 0.76. This same fluorescent spot ob-
served at same Rf value showed prominent presences
in finished product. B) HPTLC finger printing of extract
of Solanum xanthocarpum. Track 1and 2: 8 μg/ml of
Methanol extract of Panchang of extract
S.xanthocarpum. Track 3 and 4: 8 μg/ml of Methanol
extract of Cutisora tablet. Under UV 254 nm raw mate-
rials showed specific Rf values 0.01 and 0.08. These
both Rf values showed prominent presences in finished
product.
Figure 4: A) HPTLC finger printing of extract of Plumba-
go zeylanica. Track 1 and 2: 8 μg/ml of Methanol ex-
tract of bark of extract P. zyeylanica. Track 3 and 4: 8
μg/ml of Methanol extract of Cutisora tablet. Under
Visible light at 540 nm raw material showed specific Rf
values 0.01, 0.10 and 0.70. From that Rf value 0.70
showed predominant presences in finished product. B)
HPTLC finger printing of extract of Acacia catechu.
Track 1 and 2: 8 μg/ml of Methanol extract of bark of
extract A. catechu. Track 3 and 4: 8 μg/ml of Methanol
extract of Cutisora tablet. Under UV 366 nm raw mate-
rials showed specific Rf values 0.71. This same fluores-
cent spot observed at same Rf value showed promi-
nent presences in finished product.
146 ©JK Welfare & Pharmascope Foundation | International Journal of Research in Phytochemistry & Pharmacology
total ash is particularly important in the evaluation of
purity of drugs, absence of foreign inorganic matter
such as metallic salts and/or silica indicates that final
formulation is free from impurities. Result of water
soluble extractive value shows that all raw material as
well as finished product were highly soluble in water.
Result of pH was found to be within the range. Finished
product analysis showed that tablet’s net content was
within the limit of weight variation. Disintegration test
showed that tablet was disintegrated within 60 mi-
nutes. Photochemical analysis results revealed that
tablet contains alkaloid (0.74±0.06%), tannin
(7.09±0.04%), bitter (3.44±0.06%), curcuminoids
(0.39±0.14%), in significant amount.
Figure 5: A) HPTLC finger printing of extract of Curcu-
ma longa. Track 1 and 2: 8 μg/ml of Methanol extract
of rhizome of extract C. longa. Track 3 and 4: 8 μg/ml
of Methanol extract of Finish Product. Under UV
366nm raw materials showed 3 bright yellow fluores-
cent spots at specific Rf values 0.50, 0.66 and 0.70 re-
spectively. A bright yellow fluorescent spot indicated
by Rf value 0.66 showed prominent presences in fi-
nished product. B) HPTLC finger printing of Kaishor
Guggulu formulation. Track 1 and 2: 8 μg/ml of Metha-
nol extract of Powder Kaishor Guggulu formulation.
Track 3 and 4: 8 μg/ml of Methanol extract of Finish
Product. Under Visible light at 540 nm raw material
showed specific Rf values 0.28, 0.33, 0.51, 0.67, 0.75,
0.84 and 0.96. All that Rf values showed predominant
presences in finished product.
Also the data of HPTLC fingerprinting of all extracts
indicates that all extracts were derived from genuine
plants or parts of plant. Specific Rf value indicates its
presence in finished product. Heavy metals analysis
results were found within the WHO limit and Microbial
analysis results were found within the IP limit.
Conclusion
From this study, it can be concluded that Cutisora Tab-
let and all its ingredients were consistent with various
quality and purity parameters such as Organoleptic and
physico-chemical parameters. HPTLC fingerprinting
confirmed quantitative presence of raw material in
 Hitesh Gohel et al., (2013) Int. J. Res. Phytochem. Pharmacol., 3(3), 142-148

Table 6: Heavy Metal analysis

Sr. No Name of Ingredient Lead Arsenic Cadmium Mercury

Specifications NMT10ppm NMT3.0ppm NMT0.3ppm NMT1.0ppm
1 W. tinctoria Absent Absent Absent Absent
2 T. cordifolia 0.013 0.032 Absent Absent
3 A. indica 0.017 0.072 Absent Absent
4 A. vasica 0.247 Absent Absent Absent
5 T. dioica Absent Absent Absent Absent
6 S. xanthocarpum Absent Absent Absent Absent
7 P. zylenica Absent Absent Absent Absent
8 A. catechu Absent Absent Absent Absent
9 C. longa Absent Absent Absent Absent
10 Kaishor Guggulu Absent Absent 0.073 Absent
11 Cutisora Tablet Absent Absent 0.276 Absent
NMT – Not more than

Table 7: Microbial analysis

Name of Ingredient Microbial Analysis

Sr. No TBC TFC E.c P.a S.a Sal Spp
Specification 5 3
NMT10 cfu/g NMT10 cfu/g Absent
2
1 W.tinctoria 30 x10 Absent Absent Absent Absent Absent
2
2 T. cordifolia 15x10 Absent Absent Absent Absent Absent
2
3 A. indica 75 x10 Absent Absent Absent Absent Absent
2
4 A. vasica 98 x10 Absent Absent Absent Absent Absent
2
5 T. dioica 75 x10 Absent Absent Absent Absent Absent
2
6 S.xanthocarpum 15 x10 Absent Absent Absent Absent Absent
2
7 P. zylenica 38 x10 Absent Absent Absent Absent Absent
2
8 A. catechu 98 x10 Absent Absent Absent Absent Absent
2
9 C. longa 78 x10 Absent Absent Absent Absent Absent
2
10 Kaishor Guggulu 35 x10 Absent Absent Absent Absent Absent
2
11 Cutisora Tablet 19 x 10 Absent Absent Absent Absent Absent
TBC – Total bacterial count, TFC – Total fungal count, E.c – E.coli, P.a – Pseudomonas aeruginosa, S.a –
Staphylococcus aureus, Sal spp – Salmonella spp
finished product. Results of Quality control parameters Anonymous, the Ayurvedic Pharmacopoeia of India, 1st
for this Polyherbal formulation was complying with the Ed, New Delhi: Government of India, Ministry of
standards of Indian Pharmacopeia for tablet dosage Health and Family Welfare, Department of Ayush,
form. The obtained results were used to establish qual- 2008, Part I; Vol. VI.
ity of the product which ensures that there is no batch
Anonymous, Ayurved Sar Sangraha, Shri Baidyanath
to batch variation.
Ayurved Bhawan Pvt. Ltd., Nagpur, 2004, pp. 515.
Acknowledgement
Anonymous. Indian Pharmacopoeia, India: Indian
The authors like to express their gratitude to Vasu Re- Pharmacopoeia Commission, Ghaziabad, 2010, pp. 1.
search Centre, A Division of Vasu Healthcare Pvt. Ltd,
Dr. Jayendra Pandey: Dravyaguna vijnana, Vol 3,
Vadodara, for state of the art testing facility and finan-
Chowkhamba Krishnadas Acadamy, 2004, pp .801.
cial support to carry out this research work and KSKV,
Kachchha University for support and guidance Elumalai A, Chinna Eswaraiah M, Anusha V, Kiranmai K.
throughout. Phyto Pharmacological Profile of Wrighta tinctoria
International Journal of Institutional Pharmacy and
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