2100 expert_High Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.

xad Page 1 of 18

Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electrophoresis File Run Summary

Instrument Information:
Instrument Name: vk03bk10 Firmware: C.01.069
Serial#: DE04105470 Type: G2938C
Assay Information:
C:\Program Files\Agilent\2100 bioanalyzer\2100
expert\assays\dsDNA\High Sensitivity DNA.xsy
Assay Origin Path:

Assay Class: High Sensitivity DNA Assay

Version: 1.03
Assay Comments: Copyright © 2003-2010 Agilent Technologies

Chip Information:
Chip Lot #:
Reagent Kit Lot #:
Chip Comments:

l1 flg22 3 U1 mock1 U1 mock2

U1 mock3 U1 flg22 1 U1 flg22 2

U1 flg22 3 sample 8 sample 9

sample 10 sample 11

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electrophoresis File Run Summary (Chip Summary)

Sample Name Sample Rest. Stat Observation Result Result Color

Comment Digest us Label
l1 flg22 3
U1 mock1
U1 mock2
U1 mock3
U1 flg22 1
U1 flg22 2
U1 flg22 3
sample 8
sample 9
sample 10
sample 11
Ladder

Chip Lot # Reagent Kit Lot #

Chip Comments :

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
2100 expert_High Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Page 3 of 18

Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electrophoresis Assay Details

General Analysis Settings

Number of Available Sample and Ladder Wells (Max.) : 12
Minimum Visible Range [s] : 32
Maximum Visible Range [s] : 138
Start Analysis Time Range [s] : 33
End Analysis Time Range [s] : 137.5
Ladder Concentration [pg/µl] : 1950
Uses Standard Area for Ladder Fragments
Lower Marker Concentration [pg/µl] : 125
Upper Marker Concentration [pg/µl] : 75
Used Upper Marker for Quantitation
Standard Curve Fit is Point to Point
Show Data Aligned to Lower and Upper Marker

Integrator Settings
Integration Start Time [s] : 33.05
Integration End Time [s] : 137
Slope Threshold : 0.8
Height Threshold [FU] : 5
Area Threshold : 0.1
Width Threshold [s] : 0.6
Baseline Plateau [s] : 0.5

Filter Settings
Filter Width [s] : 0.5
Polynomial Order : 4

Ladder
Ladder Peak Size Area
1 35 160
2 50 210
3 100 208
4 150 221
5 200 242
6 300 270
7 400 305
8 500 306
9 600 336
10 700 321
11 1000 366
12 2000 413
13 3000 411
14 7000 400
15 10380 214

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary

Ladder

Overall Results for Ladder

Noise: 0.5

Peak table for Ladder

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 50 150.00 4,545.5 Ladder Peak
3 100 150.00 2,272.7 Ladder Peak
4 150 150.00 1,515.2 Ladder Peak
5 200 150.00 1,136.4 Ladder Peak
6 300 150.00 757.6 Ladder Peak
7 400 150.00 568.2 Ladder Peak
8 500 150.00 454.5 Ladder Peak
9 600 150.00 378.8 Ladder Peak
10 700 150.00 324.7 Ladder Peak
11 1,000 150.00 227.3 Ladder Peak
12 2,000 150.00 113.6 Ladder Peak
13 3,000 150.00 75.8 Ladder Peak
14 7,000 150.00 32.5 Ladder Peak
15 10,380 75.00 10.9 Upper Marker

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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

l1 flg22 3

Overall Results for sample 1 : l1 flg22 3

Number of peaks found: 14 Corr. Area 1: 9,977.7
Noise: 0.5

Peak table for sample 1 : l1 flg22 3

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 32 0.00 0.0
2 35 125.00 5,411.3 Lower Marker
3 44 16.95 587.8
4 128 24.60 290.3
5 242 1,577.19 9,874.0
6 270 1,425.70 7,990.8
7 283 807.16 4,315.4
8 300 563.80 2,844.4
9 315 1,166.34 5,609.7
10 346 461.34 2,018.2
11 359 1,247.11 5,261.5
12 401 940.44 3,553.8
13 452 2,355.58 7,904.8
14 10,380 75.00 10.9 Upper Marker
15 12,579 0.00 0.0
16 13,296 0.00 0.0

Region table for sample 1 : l1 flg22 3

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 9,977.7 97 382 35.4 10,687.25 49,079.8

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

U1 mock1

Overall Results for sample 2 : U1 mock1

Number of peaks found: 18 Corr. Area 1: 13,520.4
Noise: 0.5

Peak table for sample 2 : U1 mock1

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 31 0.00 0.0
2 35 125.00 5,411.3 Lower Marker
3 129 22.03 258.9
4 240 2,093.34 13,198.7
5 265 1,895.73 10,822.9
6 272 553.82 3,090.4
7 282 540.35 2,907.8
8 285 1,035.78 5,497.7
9 302 624.37 3,133.5
10 313 1,520.64 7,360.2
11 344 1,523.84 6,715.9
12 371 474.67 1,937.1
13 383 1,063.27 4,205.7
14 412 493.78 1,815.4
15 430 357.99 1,262.4
16 451 662.48 2,226.0
17 476 382.84 1,218.9
18 496 998.81 3,049.8
19 565 1,132.80 3,038.4
20 10,380 75.00 10.9 Upper Marker

Region table for sample 2 : U1 mock1

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 13,520.4 98 378 34.8 15,336.42 70,828.5

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

U1 mock2

Overall Results for sample 3 : U1 mock2

Number of peaks found: 9 Corr. Area 1: 8,515.1
Noise: 0.4

Peak table for sample 3 : U1 mock2

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 31 0.00 0.0
2 35 125.00 5,411.3 Lower Marker
3 42 40.44 1,469.8
4 251 974.00 5,876.0
5 274 1,096.40 6,067.6
6 285 1,342.20 7,133.1
7 314 1,867.87 9,001.8
8 370 3,202.02 13,105.4
9 980 8.71 13.5
10 10,380 75.00 10.9 Upper Marker
11 13,304 0.00 0.0

Region table for sample 3 : U1 mock2

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 8,515.1 98 369 30.7 8,338.49 38,165.4

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

U1 mock3

Overall Results for sample 4 : U1 mock3

Number of peaks found: 8 Corr. Area 1: 10,521.0
Noise: 0.5

Peak table for sample 4 : U1 mock3

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 31 0.00 0.0
2 35 125.00 5,411.3 Lower Marker
3 42 46.88 1,688.7
4 130 18.74 218.9
5 273 2,668.08 14,790.9
6 285 3,876.81 20,590.3
7 356 1,470.58 6,253.8
8 403 2,730.78 10,255.6
9 10,380 75.00 10.9 Upper Marker
10 12,414 0.00 0.0

Region table for sample 4 : U1 mock3

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 10,521.0 99 366 30.4 10,412.33 48,025.1

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

U1 flg22 1

Overall Results for sample 5 : U1 flg22 1

Number of peaks found: 6 Corr. Area 1: 8,469.2
Noise: 0.3

Peak table for sample 5 : U1 flg22 1

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 31 0.00 0.0
2 35 125.00 5,411.3 Lower Marker
3 42 42.83 1,540.3
4 129 99.83 1,177.1
5 272 2,036.16 11,362.4
6 286 709.42 3,752.3
7 309 6,322.08 30,994.3
8 10,380 75.00 10.9 Upper Marker

Region table for sample 5 : U1 flg22 1

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 8,469.2 97 384 33.4 8,636.36 38,832.4

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

U1 flg22 2

Overall Results for sample 6 : U1 flg22 2

Number of peaks found: 11 Corr. Area 1: 5,661.3
Noise: 0.4

Peak table for sample 6 : U1 flg22 2

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 31 0.00 0.0
2 35 125.00 5,411.3 Lower Marker
3 41 39.82 1,454.2
4 130 24.77 289.1
5 271 1,183.48 6,616.2
6 284 609.18 3,246.2
7 294 581.49 3,001.0
8 314 730.15 3,528.6
9 343 1,548.26 6,844.6
10 435 1,000.67 3,484.0
11 10,380 75.00 10.9 Upper Marker
12 12,880 0.00 0.0
13 13,641 0.00 0.0

Region table for sample 6 : U1 flg22 2

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 5,661.3 98 364 29.8 5,508.47 25,378.9

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

U1 flg22 3

Overall Results for sample 7 : U1 flg22 3

Number of peaks found: 13 Corr. Area 1: 12,869.1
Noise: 0.4

Peak table for sample 7 : U1 flg22 3

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 31 0.00 0.0
2 35 125.00 5,411.3 Lower Marker
3 41 16.19 595.8
4 128 45.48 536.5
5 294 3,067.79 15,828.1
6 309 874.77 4,290.7
7 315 474.80 2,283.6
8 323 5,997.53 28,175.8
9 480 2,366.28 7,469.9
10 2,011 4.65 3.5
11 2,364 5.77 3.7
12 3,640 5.59 2.3
13 4,363 6.61 2.3
14 4,746 17.38 5.5
15 10,380 75.00 10.9 Upper Marker

Region table for sample 7 : U1 flg22 3

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 12,869.1 98 395 31.2 12,089.91 52,119.4

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

sample 8

Overall Results for sample 8 : sample 8

Number of peaks found: 1 Corr. Area 1: 59.0
Noise: 0.5

Peak table for sample 8 : sample 8

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 10,380 75.00 10.9 Upper Marker
3 13,243 0.00 0.0

Region table for sample 8 : sample 8

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 59.0 43 397 42.3 39.38 180.9

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

sample 9

Overall Results for sample 9 : sample 9

Number of peaks found: 1 Corr. Area 1: 51.1
Noise: 0.4

Peak table for sample 9 : sample 9

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 10,380 75.00 10.9 Upper Marker
3 13,182 0.00 0.0

Region table for sample 9 : sample 9

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 51.1 37 392 43.9 38.81 181.9

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

sample 10

Overall Results for sample 10 : sample 10

Number of peaks found: 1 Corr. Area 1: 40.2
Noise: 0.3

Peak table for sample 10 : sample 10

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 10,380 75.00 10.9 Upper Marker
3 12,903 0.00 0.0

Region table for sample 10 : sample 10

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 40.2 37 377 38.4 27.07 127.9

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
2100 expert_High Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Page 15 of 18

Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Electropherogram Summary Continued ...

sample 11

Overall Results for sample 11 : sample 11

Number of peaks found: 1 Corr. Area 1: 45.6
Noise: 0.3

Peak table for sample 11 : sample 11

Peak Size [bp] Conc. [pg/µl] Molarity [pmol/l] Observations
1 35 125.00 5,411.3 Lower Marker
2 10,380 75.00 10.9 Upper Marker
3 13,221 0.00 0.0

Region table for sample 11 : sample 11

From To Corr. % of Average Size Size distribution in Conc. Molarity Co
[bp] [bp] Area Total [bp] CV [%] [pg/µl] [pmol/l] lor

200 1,000 45.6 37 386 41.3 32.61 152.4

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Gel Image

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Curves

Standard Curve

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM
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Assay Class: High Sensitivity DNA Assay Created: 7/11/2019 10:20:57 AM

Data Path: D:\...gh Sensitivity DNA Assay_DE04105470_2019-07-11_10-20-58.xad Modified: 7/11/2019 11:02:16 AM
Run Logbook

Description Number Source Category Sub Category Time Time Zone User Host
Run ended on Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019
port 2 (Number 11:02:13 AM Arab Standard 16
of wells Time
acquired: 12)
Run started on Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019
port 2 (File: 10:20:58 AM Arab Standard 16
D:\prabhu Time
heribert\2019-0
7-11\2019-07-1
1\2019-07-11\2
019-07-11\210
0 expert_High
Sensitivity DNA
Assay_DE04105
470_2019-07-1
1_10-20-58.xad
)

Product Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019

Number : 10:20:58 AM Arab Standard 16
G2938C Time
Name : Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019
vk03bk10 10:20:58 AM Arab Standard 16
Time
Vendor : Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019
Agilent 10:20:58 AM Arab Standard 16
Technologies Time
Serial# : Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019
DE04105470 10:20:58 AM Arab Standard 16
Time
Firmware : Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019
C.01.069 10:20:58 AM Arab Standard 16
Time
Cartridge : Instrument Run 7/11/2019 (GMT +03:00) Admin LAB-PC-KW-019
Electrode 10:20:58 AM Arab Standard 16
Time

2100 Expert (B.02.08.SI648) © Copyright 2003 - 2009 Agilent Technologies, Inc. Printed: 7/11/2019 11:04:05 AM