Effectiveness of Lysol with

Hydrogen Peroxide as Disinfectant

Against Escherichia coli
 INTRODUCTION
Disinfectants are used extensively in hospitals and
other health care settings for a variety of topical and
hard-surface applications. They are an essential part of
infection control practices.
 REVIEW OF RELATED
LITERATURE
Lysol is commonly used as disinfectant in the
laboratory. One of its product harnesses the versatility of
hydrogen peroxide to deliver a more powerful clean than
bleach. Lysol with hydrogen peroxide release thousands
of micro-bubbles that penetrate deep to remove tough
stains, leaving behind a fresh, clean scent with no
chemical residue. (Parsippany, 2015)
 REVIEW OF RELATED
LITERATURE
Ingredient Information
INGREDIENT FUNCTION
Water Diluent
Dipropylene Glycol Butyl Ether Solvent
Hydrogen Peroxide Antimicrobial Active
Complexing/Sequestering
Citric Acid
Agent
Sodium Methyl 2-Sulfolaurate Anionic Surfactant
C10 Ethoxylated Alcohol Nonionic Surfactant
Fragrance/Parfum Fragrance
 REVIEW OF RELATED
LITERATURE

INGREDIENT FUNCTION
Tetrasodium Complexing/Sequestering
Iminodisuccinate Agent
Capryl Glucoside Nonionic Surfactant
Hexyl Cinnamal Fragrance Component
Alkyl glycoside C10-16 Nonionic Surfactant
Linalool Fragrance Component
(Reckitt Benckiser Inc.)
Hydrogen Peroxide
The literature contains several accounts of the properties,
germicidal effectiveness, and potential uses for stabilized hydrogen
peroxide in the health-care setting. Published reports ascribe good
germicidal activity to hydrogen peroxide and attest to its
bactericidal, virucidal, sporicidal, and fungicidal properties.
Hydrogen peroxide works by producing destructive hydroxyl
free radicals that can attack membrane lipids, DNA, and other
essential cell components. Catalase, produced by aerobic organisms
and facultative anaerobes that possess cytochrome systems, can
protect cells from metabolically produced hydrogen peroxide by
degrading hydrogen peroxide to water and oxygen. This defense is
overwhelmed by the concentrations used for disinfection. (CDC,
2008)
 REVIEW OF RELATED
LITERATURE
Minimum inhibitory concentrations (MICs) are defined
as the lowest concentration of antimicrobial that will inhibit
the visible growth of a micro-organism after overnight
incubation, and minimum bactericidal concentrations (MBCs)
the lowest concentration of antimicrobial that will prevent the
growth of an organism after sub-culture on to antibiotic-free
media. MICs are used by diagnostic laboratories, mainly to
confirm resistance, but most often as a research tool to
determine the in-vitro activity of new antimicrobials. MBC
determinations are undertaken less frequently and their
major use has been reserved for isolates from the blood of
patients with endocarditis. (Andrews, 2001)
 STATEMENT OF
THE PROBLEM
The study will determine if lysol with hydrogen
peroxide is effective as disinfectant against the
bacteria, Escherichia coli.
Specifically, the following questions are needed
to be answered:
1. What is the minimum inhibitory
concentration (MIC) of lysol with hydrogen
peroxide?
2. What is the minimum bactericidal
concentration (MBC) of lysol with hydrogen
peroxide?
 HYPOTHESIS
Alternative Hypothesis
• Lysol with hydrogen peroxide is effective as
disinfectant against Escherichia coli.

Null Hypothesis
• Lysol with hydrogen peroxide is not effective as
disinfectant against Escherichia coli.
 Methodology
Preparation

Serial Dilution

Adding of Bacterial Broth

Incubation
 Methodology

INCUBATION
 Results and
Discussion
Minimum Inhibitory Concentration of
Lysol with Hydrogen Peroxide

TUBE CONCENTRATION APPEARANCE INTERPRETATION

1 1:1 Clear No Growth
2 1:2 Clear No Growth
3 1:4 Clear No Growth
4 1:8 Clear No Growth
5 1:16 Turbid With Growth
6 1:32 Turbid With Growth
 Results and
Discussion
The table showed the results of the test for the
minimum inhibitory concentration of lysol. Tube 1
containing 1:1 concentration of lysol had a clear solution
showing no bacterial growth. Tube 2, 3 and 4 containing
1:2, 1:4 and 1:8 concentration of lysol respectively,
showed the same result as tube 1. While, tube 5 and 6
with the concentration of 1:16 and 1:32 respectively, had
a turbid solution showing bacterial growth.
 Conclusion
Based on the results of the experiment, the
students conclude that lysol with hydrogen
peroxide is very effective as disinfectant and the
minimum inhibitory concentration is 1:8
concentration.
 References
Andrews, J.M. (2001). The British Society for Antimicrobial
Chemotherapy. Retrieved at
http://jac.oxfordjournals.org/content/48/suppl_1/5.abstract on
November 25, 2016
Brown , F., Dowlut S. and Karnik T. (2013). Disinfectant Efficacy
Testin. Retrieved at http://www.kvh.org.nz/vdb/document/91553
on November 25, 2016
Jozala, A.F., Mazzola, P.G., and Moriel, P. (2009). Brazilian
Journal of Pharmaceutical Sciences vol. 45. Retrieved at
http://www.revistas.usp.br/bjps/article/viewFile/10677/12445 on
November 25, 2016
 References
Chand, S., Kumar, P., and Saha, K. (2016). International
Journal of Current Microbiology and Applied Sciences
ISSN: 2319-7706 Volume 5. Retrieved at
http://www.ijcmas.com/vol-5-
1/Subhash%20Chand,%20et%20al.pdf on November 25,
2016
Andrews, J.M. (2006). Determination of Minimum
Inhibitory Concentrations. Retrieved at
http://bsac.org.uk/wp-
content/uploads/2012/02/Chapter-2-Determination-of-
MICs-2006updated.pdf on November 25, 2016
Reckitt Benckiser Inc. http://rbnainfo.com
 References
CDC (2008). Guideline for Disinfection and Sterilization in
Healthcare Facilities. Retrieved at
https://www.cdc.gov/hicpac/Disinfection_Sterilization/7_0formalde
hyde.html on November 25, 2016
Parsippany, N.J. (2015). Lysol Launches Line of Hydrogen Peroxide
Products That Marks a New Era in Household Cleaning. Retrieved at
http://search.ebscohost.com/ on November 25, 2016