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Service Manual
Table of Contents
• Proprietary Information
• Revision Status
Abbott Laboratories software programs are protected by copyright. All rights reserved.
No part off this document may be reproduced, stored, retrieved, or transmitted in any form or by any
means without the prior written permission of Abbott Laboratories.
This service manual was developed for use in the field by trained Abbott Laboratories Field Service
Representatives. The revision status of the manual is the responsibility of the manual holder.
In no event shall Abbott Laboratories or its subsidiaries be liable for any damages incurred in connec-
tion with or arising from the use of this manual by persons not fully trained by Abbott Laboratories.
The examples contained in this manual are intended for illustrative purposes only.
The revision status of the CELL-DYN® 1700 Service Manual is indicated below. It is the responsibility
of the Field Service Representative to verify that the manual contains the latest revision number of all
pages. Additional copies of this manual may be purchased.
Record document control number and sign and date this log to
provide a permanent record of revisions.
GENERAL INFORMATION
Table of Contents
1.1 SECTION OVERVIEW
1.2 PURPOSE AND SCOPE
1.3 SERVICE EXPERIENCE
1.4 OPERATING INSTRUCTIONS
1.5 HOW TO USE THIS MANUAL
Service Manual Organization
Accident Prevention Labels
Biohazard Safety Precautions
Biohazard Safety
Sharps
Biohazard Disposal
Biohazard Spills
Manual Revision Information
1.6 SYSTEM SPECIFICATIONS
Physical Specifications
Operational Specifications
List of Tables
Table 1-1 Physical Specifications (Dimensions) — CELL-DYN 1700
Table 1-2 Dimensions After Packaging for Shipment — CELL-DYN 1700
Table 1-3 Physical Specifications — CELL-DYN 1700CS
Table 1-4 Dimensions After Packaging for Shipment — CELL-DYN 1700CS
Table 1-5 Power Specifications — CELL-DYN 1700 and 1700CS
Table 1-6 Printer Input Requirements
Table 1-7 Power Consumption — CELL-DYN 1700 and 1700CS
Table 1-8 Operating Environment — CELL-DYN 1700 and 1700CS
This section provides a list of the major components and sub-assemblies of the CELL-DYN 1700, a
brief description of the remaining sections in this manual, a discussion of how to use this manual, and
an overview of the instrument’s physical, operational, and measurement specifications.
This manual contains service information for the CELL-DYN 1700 Automated Hematology Analyzer.
Included in this manual are the theory of operation, system and circuit descriptions, alignment and
verification procedures, diagrams and schematics, and board-level replacement procedures for all
major system components.
The CELL-DYN 1700 Automated Hematology Analyzer is a complex system. Analyzer performance
depends on several external components that together make up the complete hematology system.
Each system comprises the following components and subsystems:
• OPERATOR/OPERATOR TECHNIQUE (MAINTENANCE)
• REAGENT SYSTEM
— DILUENT
— DETERGENT
— LYSE
• PATIENT AND CONTROL SAMPLES
• ENVIRONMENT/POWER LINE INTEGRITY
• CELL-DYN 1700 ANALYZER
— SYRINGE ASSEMBLY
— FLUID POWER SUPPLY
— CLOSED SAMPLE ASSEMBLY
Based on experience and service history, the incidence of hematology problems and their causes
tend to occur in the same descending order of components and subsystems listed above. Note that
the majority of problems and their causes will originate with components external to the analyzer. It
follows that all external components and conditions, such as reagents, environment, integrity of sam-
ples and controls, etc., be checked and verified as correct before performing service on the analyzer.
In the investigation of any complaint, the instrument should be the last component of the system to be
suspected.
It is essential that the Field Service Representative read and understand the CELL-DYN 1700 Opera-
tions Manual, and be able to correctly perform all routine operating functions before attempting to
troubleshoot and repair the system.
DANGER
WARNING
CAUTION
NOTE: Denotes general information and helpful hints; failure to comply will present no safety, effi-
cacy, or performance issues.
T 25 T 25
TSB 60-025 TSB 60-025 Not
Installed Installed
The following symbol is used to identify the ISA containing additional information about the part or
area.
I 25
“NOTES” Page
Physical Specifications
Physical specifications for the CELL-DYN 1700 and CELL-DYN 1700CS are listed in Tables 1-1
through 1-7.
Analyzer Printer
Height 19" (49 cm) 5" (13 cm)
Width 34" (87 cm) 16 " (41 cm)
Depth 24" (61 cm) 14" (36 cm)
Weight 145 lb (66 kg) 16 lb (7 kg)
Table 1-1: Physical Specifications (Dimensions) — CELL-DYN 1700
Analyzer Printer
Height 30" (76 cm) 9" (23 cm)
Width 42" (107 cm) 22" (56 cm)
Depth 32" (81 cm) 20" (51 cm)
Weight 200 lb (91 kg) 35 lb (16 kg)
Table 1-2: Dimensions After Packaging for Shipment — CELL-DYN 1700
CELL-DYN® 1700 Service Manual 9140265A-February1995 1-12
Section 1 Search Book TOC Go Back GENERAL INFORMATION
Analyzer
Height 19” (49 cm)
Width 34" (87 cm)
Depth 26" (66 cm)
Weight 155 lb (71 kg)
Table 1-3: Physical Specifications (Dimensions) — CELL-DYN 1700CS
Analyzer
Height 30" (76 cm)
Width 42" (107 cm)
Depth 32" (81 cm)
Weight 210 lb (96 kg)
Table 1-4: Dimensions After Packaging for Shipment —CELL-DYN 1700CS
Setting Frequency
120 50/60 Hz
Table 1-6: Printer Input Requirements
Operational Specifications
Operational specifications for the CELL-DYN 1700 and CELL-DYN 1700CS are listed in Tables 1-8
and 1-9.
Aspiration
Open mode 30
Pre-Dilute mode 40
Closed mode 450
Table 1-10: Aspiration Volumes
Parameter CV%
WBC < 2.5
RBC < 1.7
HGB < 1.2
MCV < 1.5
PLT < 6.0
MPV < 6.0
Table 1-17: Within Sample Precision of the Hemogram Parameters — Open Mode
Parameter CV%
WBC < 2.7
RBC < 1.7
HGB < 1.2
MCV < 1.5
PLT < 6.0
MPV < 6.0
Table 1-18: Within Sample Precision of the Hemogram Parameters — Closed Mode
THEORY OF OPERATION
TABLE OF CONTENTS
2.1 SYSTEM OVERVIEW
2.2 SYSTEM DESCRIPTION
2.3 PURPOSE OF SYSTEM
2.4 SAMPLE PREPARATION
2.5 SAMPLE TRANSPORT
Open Sample Mode
Pre-Dilute Mode
Closed Sample Mode
Sample Dilution
2.6 PARTICLE DETECTION
2.7 PULSE AMPLITUDE TO PARTICLE SIZE RESPONSE
2.8 SIZE THRESHOLD AND CELL CHANNELIZATION
2.9 METERED VOLUME
2.10 RBC, WBC, AND PLT HISTOGRAM GENERATION
LIST OF FIGURES
Figure 2-1 CELL-DYN 1700 Basic Block Diagram
Figure 2-2 Electrical Impedance Detection
Figure 2-3 Pulse Amplitude to Particle Size Response
Figure 2-4 Meniscus Detection
Figure 2-5 Histogram Generation
Figure 2-6 Simplified Hemoglobin Block Diagram
This section provides a brief review of the principles of operation for the CELL-DYN 1700, including
sample preparation, sample aspiration, the flow system, and the measurement process.
The CELL-DYN 1700 is basically a particle counter dedicated to the electronic detection and mea-
surement of blood cells contained in a sample of whole blood. Blood cells are particles which exhibit
the special quality of being electrical insulators. In whole blood, these particles are suspended in a
conductive medium commonly called plasma. It is these two natural electrical qualities that permit the
electronic measurement of the following:
• The number of cells per unit of volume
• The size of each cell
• The size distribution of all cells contained in a sample.
A simplified diagram of a particle counter is shown in Figure 2-1. The major functions of an electronic
particle counter are as follows:
• Sample transport (Flow System)
• Particle detection (Transducer)
• Pulse amplitude to particle size response (Amplifier)
• Size thresholds (Discriminators) and cell channelization (A/D converter)
• Sample volume metering (Metering System).
The purpose of this system is to convert the size of each detected particle to an electronic equivalent
signal. This signal is then processed to calculate the number of particles within a pre-selected size
range for a known sample volume. The displayed value represents the concentration of the sample in
cells per microliter (cells/µL).
Hemoglobin is measured by a separate colorimetric method. The absorbance, calculated from the
measured values of light transmission, is directly proportional to the concentration of hemoglobin.
A description of each major function of the instrument necessary to accomplish this task follows.
A major disadvantage of whole blood measurement relative to electronic particle counting is the high
concentration of cells in whole blood. This problem is easily solved by controlled dilution. A prerequi-
site for electronic particle detection is low sample concentrations that will permit the existence of only
one particle in the sensing zone at any given time. Two or more cells in the sensing zone will be
detected as a single cell and result in a counting error. The high concentration of cells in whole blood
requires accurate dilution before electronic measurement can be attempted. When the dilution ratio is
known, the value measured by the instrument can be related to the whole blood value.
An obvious question is how much dilution of whole blood is required to satisfy the requirement of sin-
gle cell detection in the sensing zone?
Sample Dilution
A 30 µL sample of whole blood is drawn into the Sample Probe and mixed with 7.5 mL of diluent to
make the primary dilution. A second 100 µL is then aspirated from the primary dilution to make the
secondary 1:12,801 RBC/PLT dilution. The primary dilution is then mixed with 1 mL of lyse to com-
plete the 1:285 WBC/HGB dilution. The vacuum system transports the primary and secondary dilu-
tions through the WBC and RBC apertures and HGB Flow Cell.
The flow system is then flushed and made ready for the next sample.
A transducer employing the electrical impedance principle is used for detection. This function per-
forms the conversion of the physical properties of a detected cell to an electronic equivalent signal.
The continuous passage of cells through the aperture's sensing zone produces a pulse train at the
output of the amplifier. The gain control of the amplifier calibrates the sizing function of the instrument
by establishing a known relationship between the mean size of the cells and the mean pulse ampli-
tude of the signal. This linear response is depicted in Figure 2-3.
Figure 2-1, shown earlier, is a basic block diagram of the measurement and metering circuitry for
RBC, WBC, and PLT.
+ VOLTS
GAIN
APERTURE
SIGNAL OUT
AMPLIFIER
SAMPLE DETERGENT
TRANSDUCER
The measurements require a known, repeatable sample volume. The instrument performs this func-
tion by optical detection of the leading edge of a liquid column (meniscus), as depicted in Figure 2-4.
The light transfer efficiency between an IR (infrared) light source and a photo transistor is controlled
by the optical characteristics of a glass metering tube in the light path. In the absence of liquid, as
shown in State A, the metering tube contains air and reduces the transfer of light by the refraction of
the glass walls and the density of the air within the glass tube.
With reference to State C, the metering tube is filled with liquid. The level of refraction is reduced by
an increase in optical density of the liquid, and a small increase in light transfers the results.
A third state will momentarily occur during the transition of the meniscus through the light path. As
shown in State B, the light transfer efficiency is greatly reduced when the light path is deviated by
refraction as well as reflection qualities of the concave meniscus. It is this phenomenon that serves
as a leading edge detector.
As stated earlier, each parameter has 256 channels available. The width of each channel is as fol-
lows:
RBC = 1.00 cubic microns
WBC = 1.758 cubic microns
PLT = 0.137 cubic microns
The RBC will be used as an example since it has a 1:1 relationship.
Figure 2-5 illustrates a smoothed RBC histogram and an enlarged view of the raw counts per channel
of the peak portion of the histogram (section “A”).
If we compare section “B” with section “A”, we can see the relationship of channel data to the actual
histogram shape. The raw counts increase, with volume, on the leading edge and decrease on the
trailing edge.
2.11 HEMOGLOBIN
A simplified hemoglobin system is shown schematically in Figure 2-6. The concentration of hemoglo-
bin in the prepared sample is measured in grams per deciliter. This concentration is proportional to
the absorbance of the light in the green, 540 nanometer wavelength region.
A clear reference solution (detergent) is first measured in the HGB Flow Cell. A prepared sample con-
taining hemoglobin is measured next. Hemoglobin concentration is determined by subtracting the
logarithm of the voltage of the measured sample from the logarithm of the voltage of the clear refer-
ence solution.
A light path through the transparent flow cell is formed from the light source, a 540 nanometer interfer-
ence filter, and a photo detector.
The output current from the photo detector, which is proportional to the light energy received, is ampli-
fied by the current-to-voltage amplifier and provides an output signal.
SYSTEM DESCRIPTION
Table of Contents
3.1 SECTION OVERVIEW
3.2 SYSTEM SUB-ASSEMBLIES
Flow Panel
Fluid Power Supply
Reagent Inlet Panel
Syringe Assembly
Electronics Card Cage Assembly
CRT and Keyboard
Power Supply Module
3.3 MAJOR SUBSYSTEM DESCRIPTIONS
Data Interface and Control Subsystem
Measurement Subsystem
Solenoid and Motor Subsystem
User Interface Computer Subsystem
Data Link Adapter (DLA)
IDE Controller and I/O Board
Input/Output Board
List of Tables
Table 3-1 PC Motherboard Configuration
List of Figures
Figure 3-1 Data Interface and Control Board Diagram
Figure 3-2 Measurement Block Diagram
Figure 3-3 Solenoid and Motor Drive Block Diagram
Figure 3-4 User Interface Computer
Figure 3-5 Power Distribution Block Diagram
This section contains a system overview, as well as information on the major subsystems. For a more
detailed description of the CELL-DYN 1700 parameters, reagents, specifications, and operation, refer
to the CELL-DYN 1700 Operations Manual.
Flow Panel
The Flow Panel consists of the majority of tubing and hardware for sample processing.
Syringe Assembly
The Syringe Assembly includes the Sample Syringe for aspirating samples, the Diluent Syringe for
supplying diluent throughout the flow system, and the Lyse Syringe for dispensing lyse during the
HGB measurement process.
To aid in understanding the overall system, the electronic modules are divided into the following major
functional subsystems:
• Data Interface and Control Subsystem
• Measurement Subsystem
• Solenoid and Motor Drive Subsystem
• User Interface Computer Subsystem
• AC and DC Power Distribution Subsystem
Measurement Subsystem
The measurement subsystem provides detection, amplification, and processing of the signals from
the von Behrens RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/
PLT and WBC metering is also included in this subsystem.
Refer to Figure 3-2 for a diagram of the measurement process.
CIRCUIT DESCRIPTIONS
Table of Contents
4.1 SECTION OVERVIEW
4.2 PRE-AMPLIFIER MODULE (PAM)
4.3 MAIN AMPLIFIER MODULE (MAM)
4.4 SIGNAL PROCESSOR MODULE (SPM)
4.5 CELL COUNT MODULE (CCM)
Microprocessor Section
System Clock
6809E Microprocessor Support Circuits
PROM
RAM
VIA (Versatile Interface Adapter)
DMA (Direct Memory Access) Section
Pulse Height Memory (PHM)
Cell Counters
DMA Timing and Control
Interface to Microprocessor Bus
4.6 METERING MODULE
List of Figures
Figure 4-1 Cell Pulse Classifications
Figure 4-2 MAM (Main Amplifier Module) Block Diagram
Figure 4-3 SPM (Signal Processor Module) Block Diagram
Figure 4-4 DLA (Data Link Adapter) Block Diagram
This section contains a description of the circuitry for the following printed circuit boards:
• Pre-Amplifier Module (PAM)
• Main Amplifier Module (MAM)
• Signal Processor Module (SPM)
• Cell Count Module (CCM)
• Metering Module
• Device Control Module (DCM)
• Cable Distribution Module (CDM)
• Solenoid Driver Module (SDM)
• Motor Processor Module (MPM)
• Stepper Drive Printed Circuit Board (SDP)
• Pressure/Vacuum Regulator Module (PVRM)
• Linear Power Supply Module
• PC Power Supply Module
• 24V Switching Module
• Pump Relay Module (PRM)
• User Interface Computer (UIC)
• Data Link Adapter (DLA).
Refer to Figure 3-2 in Section 3 and Figures 4-1 and 4-2 in this section.
The Main Amplifier Module consists of the following major circuits:
1. WBC differential amplifier and main amplifier
2. RBC/PLT differential amplifier and main amplifier
Microprocessor Section
System Clock
The CCM uses an 8 MHz oscillator (U10) that is divided by eight by a Johnson counter (U20) to pro-
vide 1 MHz system clocks for the 6809E microprocessor. The signals E and Q are provided to the
6809E by the Johnson counter. VUA (Valid User Address) is provided to pin 10 on the motherboard.
CELL-DYN® 1700 Service Manual 9140265A-February1995 4-12
Section 4 Search Book TOC Go Back CIRCUIT DESCRIPTIONS
6809E Microprocessor Support Circuits
The data and address buffers of the 6809E are buffered by an LS640 (U33) inverting bi-directional
buffer for the data bus and two LS244 octal buffers (U34, U35) for the address bus. Address decod-
ing is done by four LS139 2 to 4 decoders (U23, U21).
A 555 timer (U12) is used for the 6809E power-up reset. The 6809 may also receive an external reset
signal via pin 14 on the edge connector. This is the system-wide reset.
PROM
The EPROM used on the CCM (U37) is a 27C256, for 32K by 8 of program storage. A strappable
jumper selects the EPROM type.
RAM
The program RAM used on the CCM (U36) is a HM6264 (or equivalent), for 8K by 8 of program data
storage. A strappable jumper selects the RAM type. Jumper J5 must be set to 8K.
VIA (Versatile Interface Adapter)
The LSI (Large Scale Integration) interface used on the CCM is a 6522 VIA (U13). This device per-
forms a number of functions, as described below:
1. CCM Real Time Clock
One of the two 16-bit timer/counters in the VIA is used for the CCM real time clock. This time
base is always programmed to 1 millisecond (in current applications); it presents a repetitive
FIRQ interrupt to the 6809E. All process control functions, e.g., flow system timing, stepper
motor motions, sensor scanning rates, etc., are based on this timer. There should always be a
1 KHz frequency at test point TP5.
Refer to blocks 20, 23, and 24 off the MPM in Figure 3-3 in Section 3.
The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives
a winding of the Stepper Motor. Bits I0 and I1 are used to control four motor current levels:
1. P0 - High Current
2. P1 - Medium Current
3. P2 - Low Current
4. P3 - Current Off
Bits PH0 and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+
and Feedback- are used to generate a motor self-test.
The Linear (Main) Power Supply module generates the following voltages:
1. +12VDC — Cable Distribution Module
2. +24VDC — Cable Distribution Module
3. +100VDC — Pre-Amp Module
4. +15 & -15VDC — Analog Circuit Boards
Refer to Figure 3-5 in Section 3.
The +24VDC Switching Module regulates power to the Motor Processor Module, via the PDM, to drive
the Stepper Motors. Refer to Figure 3-5.
The User Interface Computer (UIC) is designed around an Intel-compatible 386SX/DX chip running at
25 — 40 MHz. (Future configuration may be a 486DX chip running at 33 or 66 MHz.) The UIC
receives power from the Switching Power Supply via the Power Distribution Module and receives sta-
tus and measurement data from the CCM. The board contains EPROM, CMOS RAM, input/output
circuitry for the interface ports, and addressing circuitry.
The following components, described in Section 3 of this manual as part of the User Interface Com-
puter, are purchased OEM from commercial vendors:
The Data Link Adapter provides interfaces from the 386/486 computer to both the CCM (Cell Count
Module) and the instrument (membrane) key panel. These two interfaces function independently,
under application software control. However, some of the circuits on the DLA board are shared. The
DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to Figure 4-4 for an illustration of the
DLA board.
Interface to CCM
A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel inter-
face that is software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (D0 —
D7) in one direction at a time. The UIC (User Interface Computer) initiates an inquiry message hand-
shake on a periodic basis or when it has a command to send. The CCM responds by sending data or
replying to the command sent.
Table of Contents
5.1 SYSTEM OVERVIEW
5.2 DIAGNOSTICS MENU USAGE
Level One
Level Two
Level Three
Level Four
5.3 FAULT REPORT DESCRIPTION
5.4 CELL-DYN 1700 TROUBLESHOOTING GUIDE
Troubleshooting Chart
Nonfunctional Instrument Problems
Video Display Problems
Displayed Error and Fault Problems
Data Problems
Clog and Flow Error Problems
Miscellaneous Problems
5.5 RAW DATA DESCRIPTION
Raw Data DisplayDescription
List of Tables
Table 5-1 Disk Drive Setup Information
Table 5-2 DEC Service Commands
Table 5-3 Motor Power Specifications
Table 5-4 Motor Direction Commands
Table 5-5 Motor Speed Commands
Table 5-6 Error Messages
Table 5-7 Event Messages During Diagnostic Menu Count Test
Table 5-8 Operator-Correctable Alarm or Fault Messages
Table 5-9 Summary of Error Messages
List of Figures
Figure 5-1 Smoothing ON/OFF Example
Figure 5-2 Raw Data Example
Figure 5-3 AMI BIOS
Figure 5-4 Motor Power Test
Figure 5-5 Probe Up/Down Initialize and Run Cycle
Figure 5-6 Probe Initialize Mode
This section is designed to aid the service representative in the troubleshooting and repair of the
CELL-DYN 1700/1700CS System. Emphasis is placed on using various System Status and Fault
Reports, which can be accessed by the service representative, to solve problems. Special Service
Commands are also available to exercise and observe mechanical and electronic functions.
Utilization of the DIAGNOSTICS Menu enables the operator or service representative to identify and
correct both operator-correctable and service-correctable faults. When the computer senses a fault,
the message <NOT READY: SEE DIAGNOSTICS> is displayed in the System Status Box on the
RUN Menu. The following keys are available in the DIAGNOSTICS Menu.
Level One
• INITIALIZATION: Used to perform an Initialization cycle: returns movable components to
"home" position and performs internal self-tests.
• RAW DATA: Used to display raw measurement data for the last specimen.
• COUNT TEST: Used to run specimens without returning to RUN Menu and display
Raw Data.
• MORE: Used to display additional functions.
• PRINTER OUTPUT: Used to toggle printer output ON and OFF.
Level Three
• PROBE HOME: Moves Sample Probe up and above RBC Cup.
• PROBE UP: Moves Sample Probe up.
• MORE: Used to display additional functions.
• PRINTER OUTPUT: Used to toggle printer output ON and OFF.
A detailed list of all faults generated by the CELL-DYN 1700 software and hardware is contained in
Section 5.10. The fault classifications reported in the Fault Report primarily contains data pertaining
to the last CCM fault.
If a fault occurs, pressing the [HELP/ERROR] key will immediately display the Fault Log in the DIAG-
NOSTICS Menu. This log may contain up to 16 faults, with the current fault leading the list. An alter-
native procedure is to go to the MAIN MENU and press [DIAGNOSTICS]. In this case, the Fault
Report, not the Fault Log, is immediately displayed.
A list of symptoms, probable causes, and corrective actions for the most common problems encoun-
tered on the CELL-DYN 1700 is given in the Troubleshooting Chart below. The probable causes and
corrective actions are arranged in descending order from most likely to least likely. When trouble-
shooting a problem, start with the most likely cause first.
If possible, thoroughly verify that a component is defective before replacement. Some problems can
be verified visually, but other problems require a DVM (Digital Volt Meter).
When troubleshooting "DATA PROBLEMS", only the measured parameters RBC, PLT, WBC, HGB,
and MCV should be used for reference. Using the calculated parameters can become confusing
when trying to isolate a problem.
When troubleshooting "CLOG AND FLOW ERROR PROBLEMS", refer to Figure 8-1 in Section 8 for
the "MIN" and "MAX" specifications for the RBC and WBC Upper (T1) and Lower (T2) times.
From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key will
display raw data obtained from the last count cycle.
When a single count is done, all data is contained in the first column. When a PLT recount occurs,
data from the first cycle appears in column #2 and data from the recount appears in column #1.
The seven LEDs on the CELL-DYN 1700 CCM can reveal much about the fundamental CCM and
overall machine state. In general, the LEDs indicate whether the CCM is in a normal functioning
mode or in a fault state, and in either case, help to characterize the CCM state. Also, one of the LEDs
gives some information about the state of the UIC as well.
The CCM tests itself on power-up. These fundamental tests include ROM, RAM, and VIA. If any test
fails, the CCM will attempt to execute a routine which will flash the green LED on the board. Also, it
will place a 4-bit fault code into the adjacent yellow LEDs.
LED Notation
- = LED is off
g = green LED on, not flashing
gs = green LED flashing slowly (approx. 1 Hz)
y = yellow LED on, not flashing
yf = yellow LED fast-flickering
ys = yellow LED flashing at slow, non-periodic rate
Power-on Tests
1. gs - - - y - y CCM failed ROM test, on 1st checksum byte.
2. gs - - y - - y CCM failed ROM test, on 2nd checksum byte.
3. gs - - y y - y CCM failed VIA test, register checked
(DDRA).
4. gs - y - - - y CCM failed VIA test, register checked (IER).
5. gs - y - y - y CCM failed VIA test, register checked (IFR).
6. gs - y y - - y CCM failed VIA test, register checked (VCR).
7. gs - y y y - y CCM failed VIA test, register checked (DDRB).
8. gs y - - - - y CCM failed RAM test, walking 1's.
9. gs y - - y - y CCM failed RAM test, on clearing to zero.
CMOS Setup
The CMOS Setup contains all the information needed by the BIOS system to establish proper com-
munications between the motherboard and the various computer system devices. The configuration
for the current motherboard is listed below:
Part No.
Current motherboard 1700019
IDE hard drive 2005712
IDE controller 1700021
Floppy
Disk Drive NA NA NA NA NA 1.44
3.5”
Setup Screen
The Setup screen is the user interface to the Basic Input-Output System (BIOS) which resides on the
battery backed-up CMOS RAM chip on the motherboard. The Setup screen is used to input the hard-
ware configurations of the various devices, so that the BIOS can set up proper communications within
the computer.
The BIOS currently shipped on the CELL-DYN 1700 was developed by American Megatrends Inc.
(AMI). The AMI BIOS setup is illustrated in Figure 5-3.
Discussion
Several commands are available to initiate individual actions in the CELL-DYN 1700 hardware and
software. These commands are used for troubleshooting and/or alignment when a single action is
desired or required to be repeated several times.
The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press
[DIAGNOSTICS] followed by [SERVICE DEC CODE]. When this softkey is pressed, the message
<SERVICE FUNCTION ONLY: ENTER COMMAND:> is displayed.
A command can now be entered. Pressing the Enter key on the keyboard will initiate the action. Only
one command can be entered at a time and [SERVICE DEC CODE] must be pressed before a com-
mand is entered.
All commands available by direct softkey can be accessed by pressing [MORE].
NOTE: Use only the commands listed in Table 5-2 and always verify that the correct number has been
entered before initiating the action. Use only those numbers listed below. Other numbers may
CELL-DYN® 1700 Service Manual 9140265A-February1995 5-33
Section 5 Search Book TOC Go Back DIAGNOSTICS AND TROUBLESHOOTING
refer to engineering commands which are not to be used in the field and which may cause dam-
age if used improperly. Be fully aware of the purpose of any of the following commands before
using them. This is a direct-activation method which should be used with caution because the
physical state of the CELL-DYN 1700 may not be in agreement with the function to be per-
formed. After using service commands, always re-initialize the system by turning the power
OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to
ensure the instrument is in the proper configuration for normal operations.
Under Advanced CMOS Setup, pressing the F6 key displays the default settings. The settings
displayed in Figure 5-3 above are set at default except for the bolded settings, which have been
changed for the CELL-DYN 1700.
The normal system bootup sequence is drive C:, A:. During instrument service, if it is neces-
sary to boot from the floppy disk, change the bootup sequence to A:, C:. When service is com-
plete, the sequence must be changed back to C:, A:.
The motors that enable the Sample Probe to move up/down and to rotate are stepper motors which
are under direct computer control. Since there is no direct positional feedback sent to the computer,
position switches are employed to verify critical positions during normal operation. It is important to
understand that these switches only verify and do not control the movement of the Sample Probe.
In the DIAGNOSTICS Menu, Service DEC Codes 128, 129, and 130 allow the service representative
to control and exercise all stepper motors in the CELL-DYN 1700. This description will focus on the
Probe Up/Down Motor (B/2) and the Probe Rotate Motor (C/3) which control the movement of the
Sample Probe.
Speed in Steps
Command
per Second
1 50
2 75
3 283
4 300
5 166
6 200
7 250
8 10
9 151
10 222
11 25
12 182
13 100
14 125
15 91
16 67
17 111
8 STE PS PR E -M IXIN G C U P R BC
8 STEPS
M IXIN G
C H AM B ER
D ISPEN SE PO SITIO N ELEC TR O N IC H O M E
117 STE PS
112 STE PS
C EN TER PO S IT IO N
H ARD STO P
3 3
AD JU ST
SW. #3 H ER E DISPENSE POSITIO N
8 3
P RO B E SH A K E
AD JU ST SW. #4 H ER E
D IS P E N SE P O S ITIO N
245
119
112
C EN TER PO SITIO N
Table 5-6 below lists the most serious error messages on the CELL-DYN 1700 instrument.
The user has entered a command for the CCM whose numeric value
Code N is
exceeds 127. The value entered was N.
Invalid
Incomplete On a CELL-DYN 1700CS, not enough blood was detected for the
Aspiration last processed sample.
Error Message
Description
(Status box)
Cannot do this The user has attempted to issue a command to the CCM that cannot
Function be executed because of a pending fault condition.
WBC Meniscus
Detection, RBC During the most recent count, a meniscus was not detected or was
Meniscus detected at an unexpected time.
Detection
WBC Count
Time-out (clog),
During the most recent count, a Clog occurred.
RBC Count
Time-out (clog)
Other error-
related mes-
sages:
An error in CCM/MPM interprocessor communications occurred. A
MPM to CCM, fault was generated in an attempt to send or receive motor or other
Message Trans- command to or from MPM, or the MPM was unable to perform the
mit Error function.
Command to
be Sent to
MPM is Incor-
rect
Error Message
Description
(Status box)
Count Test
The [COUNT TEST] key in the DIAGNOSTICS Menu is used to run specimens and display Count
Check data without returning to the RUN Menu. Coded data relating to specific cycle functions, raw
measurement, and flow count time are displayed for use in troubleshooting or service.
Table 5-7 lists the event messages that are displayed during the Diagnostic Menu Count Test.
DOS Errors
Arithmetic overflow
Bad drive request structure length
Cannot remove current directory
Cannot rename across drives
Collection index out of range
Collection overflow
CRC error in data
General
System Fault
Not Ready: See DIAGNOSTICS
Uninitialized
Normal Operator-Correctable
Detergent empty
Diluent empty
Lyse empty
Waste full
DLA Faults
Date
Battery failure or system date/time not set
Disk I/O
Cannot open CD1700. ini configuration file
QC Log
Can not accept specimen
Can not reject specimen
No loading, QC file has to be empty
Purge log failed
Read QC file failed
Data Log
Cannot do if uninitialized
Cannot write Data Log header to disk
Count overrange
Data Log write error
Failed to read from Data Log
Flow err
Initialize
Data Log initialization failed
QC Log initialization failed
Communications
Break interrupt
Comm open error
Comm error
Framing error
Printer Driver
Printer Fault
Printer Not Ready
Printer Off-line
Printer Out Of Paper
Printer Time-out
Ticket Printer Not Ready
QC Log I/O
Failure to read from QC Log
Failure to write to QC Log
Help
Unable to open help file
Reagent Log
Unable to create file
Unable to open file
X-B File
Failed to write to Data Log Header
Failed to write to Data Log
No response from CCM
General Faults
Abnormal time-out / no MPM response
Attempt to send MPM a new command while busy
CCM pulse height memory saturation warning
Fault Log
Error in writing to Fault Log file
Error in writing header to Fault Log file
Lab ID Setup
Drive A is not ready
Fail to read assay file
Fail to write LAB ID file
Incorrect assay format
Incorrect disk. QC values do not apply to this instru-
ment
Incorrect Exp. Date
Incorrect Lot Number
Incorrect parameter in assay file
CD1700 (subdirectory)
CD1700.EXE
CD1700.INI
DATALOG (20.5 MB)
QCLOG (5.5 MB)
FAULTLOG
REAGLOG
ID.DAT
HELP (subdirectory)
HELPxx (xx is numeric)
Accessing DOS
There are two ways to access the DOS program from the CD1700 program: with the CD1700 pro-
gram still loaded or by exiting the CD1700 to DOS. The Exit CD1700 to DOS method is the preferred
method.
Exiting DOS
To exit the DOS Program and return to the CD1700 Program in the case where the CD1700 Program
remains loaded (Alt d was used to access DOS), type exit at the C prompt and press the Enter key.
The DIAGNOSTICS Menu will again be displayed.
For example, to stop the file names at the end of each page: type dir /p and press the Enter
key.
To display the file names in columns across the width of the page: type dir /w and press the
Enter key.
Software Installation/Upgrades
1. 1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.
2. 2. Press ESC on the PC keyboard. A warning message appears on the screen. Heed
the message.
3. 3. Press the Alt and x keys simultaneously. The following DOS prompt appears:
C:\CD1700>.
4. 4. Install the software disk in Drive A.
5. 5. For initial software installation only, type A:\ncs for the 1700 model or A:\cs for the
Table of Contents
6.1 SECTION OVERVIEW
This section contains diagrams and schematics for the CELL-DYN 1700 and 1700CS.
Table of Contents
7.1 INTRODUCTION
7.2 SAFETY PRECAUTIONS
Decontamination
Decontamination Procedures
Printed Circuit Board Handling
7.3 SERVICE EQUIPMENT REQUIRED
7.4 DISASSEMBLY/REPLACEMENT PROCEDURES
Model 1700CS Upper Front Cover Removal
Model 1700 Upper Front Cover Removal
Model 1700CS Lower Front Cover Removal
Model 1700 Lower Front Cover Removal
Top Cover Removal
Bezel Removal
Right Cover Removal
Left Cover Removal
RBC and WBC Aperture Plate Removal
von Behrens WBC and RBC/PLT Transducer Removal
WBC and RBC Metering Board Removal
Table of Contents
8.1 SECTION OVERVIEW
8.2 TEST EQUIPMENT AND SUPPLIES REQUIRED
8.3 PREPARATION FOR ALIGNMENT/VERIFICATION
8.4 ORDER OF ALIGNMENT/VERIFICATION
8.5 VACUUM AND PRESSURE ADJUSTMENTS
Regulator Alignment
Pressure Adjustment (0.5 psi)
Pressure Verification (High)
Vacuum Adjustment (8 inch)
8.6 METERING SYSTEM TIMING ADJUSTMENTS — RBC AND WBC
RBC Metering System Timing Adjustment
WBC Metering System Timing Adjustment
8.7 24V SWITCHING MODULE ADJUSTMENT-
8.8 POWER DISTRIBUTION MODULE TEST POINTS
8.9 CABLE DISTRIBUTION MODULE TEST POINTS
8.10 SIGNAL PROCESSOR MODULE ADJUSTMENT
List of Figures
Figure 8-1 CELL-DYN 1700 Metering Timing Chart
Figure 8-2 RBC Metering Tube
Figure 8-3 WBC Metering Tube
Figure 8-4 PDM Test Points
Figure 8-5 SPM Test Points
Figure 8-6 DCM Test Points
Figure 8-7 PAM Test Points
Figure 8-8 MAM Test Points
Figure 8-9 WBC Gain Adjustments
Figure 8-10 RBC Gain Adjustments
This chapter discusses the alignment and verification procedures for the CELL-DYN 1700. These
procedures are used to ensure the proper electronic alignment of the circuitry.
These procedures also serve as a method of isolating a defective assembly, module, or printed circuit
board.
Service representatives must ensure that all external components of the system, such as reagents,
blood samples used, controls and calibrators, environment, and AC power, are acceptable and cor-
rect before proceeding with the alignment and verification procedures.
Perform the following procedure to prepare the CELL-DYN 1700 for alignment/verification:
1. Verify all reagents are correct and available in sufficient quantities to perform 100-150
cycles on the instrument.
2. Remove the upper and lower front covers, left and right side covers, and top cover.
3. Remove and clean both RBC/PLT and WBC aperture plates following the procedure in
Section 9: Service and Maintenance, Subsection: Aperture Plates Cleaning of the
CELL-DYN 1700 Operations Manual.
4. Clean the HGB Flow Cell following the procedure in Section 9, Subsection: HGB Flow
Cell Manual Cleaning of the CELL-DYN 1700 Operations Manual.
5. Re-initialize the instrument by turning the system OFF then ON again. When
Initialization is complete, press [PRIME/RUN] to prime the instrument. Observe the flow
system for leaks, tubing placement, pinched tubing, etc.
6. Reinstall the covers on the instrument.
7. Run a Background count. Verify all background values are within the following
specifications:
WBC < 0.5 K/µL
RBC < 0.05 M/µL
HGB < 0.1 g/dL
PLT < 10.0 K/µL
8. Enter the date and time according to directions in Section 2: Installation Procedures
The following procedures are presented to ensure proper alignment of the CELL-DYN 1700.
1. Vacuum and Pressure Adjustment (Section 8.5)
2. RBC Count Time Adjustment (Section 8.6)
3. WBC Count Time Adjustment (Section 8.6)
4. 24V Switching Module Adjustment (Section 8.7)
5. Power Supply Voltage Checks and Adjustment (Section 8.8)
6. Signal Processor Module Alignment (Section 8.10)
7. Device Control Module Alignment (Section 8.11)
8. Pre-Amplifier Module Alignment (Section 8.12)
9. Main Amplifier Module Alignment (Section 8.13)
The CELL-DYN 1700 utilizes one vacuum and two pressure levels to accomplish the following tasks:
moving sample, reagents, and waste, bubble mixing of sample, and backflushing RBC and WBC
apertures. The vacuum and bubble mix pressures are adjustable by a solid-state regulator. The
backflush pressure is not critical and the pump is under direct computer control.
The solid state regulator has two input ports: P1 for pressure and P2 for vacuum. It also has jumper
terminals which accommodate all desired vacuum and pressure ranges.
Regulator Alignment
This offset adjustment must be performed with no pressure or vacuum applied to the regulator. Fol-
low the steps below:
1. Disconnect the appropriate pumps on the pump relay module and bleed off pressure
from the accumulators.
• J4 for low pressure
• J2 for vacuum on new-style pumps
2. Remove the pressure or vacuum line from the top of the regulator.
3. On the Pump Relay Board, the voltage should be 5.0 +/- 0.15 volts at J7 pin 7 for
vacuum, and J8 pin 5 for pressure.
NOTE: You must remove the board mounting screws and free the rest of the board to make the re-
maining adjustments. Disconnect J6 or J7 while relocating the appropriate printed circuit board
to avoid shorting out components.
4. Note the current position of jumper E1 and set E1 to the C-D position.
The instrument uses the Volumetric Metering process to regulate the count cycle and to ensure that a
precise volume of sample is analyzed for the measurement. Table 8-1 shows the results of a fault
report displayed on the screen if a flow error or clog occurs during a Run cycle. Figure 8-1 illustrates
the timing relationships for WBC and RBC measurements.
For each transducer there are two distinct counting periods, T1 and T2. Figures 8-2 and 8-3 illustrate
the counting periods for the RBC and WBC metering tubes, respectively.
The 24V Switching Module is located next to the fan behind the CRT assembly.
Adjust the regulated +24V supply to 24.5 +/- 0.5V.
Location Function Voltage
BROWN +24V 25.0V +/- 1.0
NOTE: The 24V Switching Module has only one potentiometer. Adjust the potentiometer with an in-
sulated screwdriver and record the adjustment.
Table 8-2 lists the test points located on the Power Distribution Module (PDM) and Figure 8-4 illus-
trates the test points on the PDM board.
Table 8-3 lists the test points located on the Cable Distribution Module (CDM).
The Signal Processor Module (SPM), located in the card cage on the right side of the instrument, con-
tains the circuitry for the RBC and WBC lower fixed discriminators and the lower and upper platelet
discriminators. A discussion of the functions of the SPM can be found in Section 4 of this manual;
however, the only field adjustments recommended on this board are the discriminator voltages.
Follow the steps below to align the SPM:
1. Verify that the instrument is in the READY state.
The Device Control Module (DCM) is located in the main electronics card cage. The DCM has a sin-
gle adjustment that can be performed in the field. The adjustment is for the D to A converter output.
No other adjustments are required.
Follow the steps below to align the DCM:
1. Verify the instrument is in the READY state.
2. Connect the DVM positive lead to TP3 on the DCM. Connect the ground lead to TP2
(DAC GND) on the DCM board.
3. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Press
[SERVICE DEC CODE], type ”2” and press Enter.
4. Adjust R1 for 9.0 volts +/- 0.07 volts.
5. Press [SERVICE DEC CODE], type “1” and press Enter.
6. Check TP3 for 4.5 volts +/- 0.07 volt.
NOTE: Counterclockwise rotation increases the voltage.
The Pre-Amplifier Module (PAM) is located on the upper right corner of the Flow Panel. Hemoglobin
circuitry, and the PLT and WBC aperture currents require verification and/or adjustment on this mod-
ule. The HGB Flow Cell should be cleaned before performing hemoglobin alignments (refer to Sec-
tion 9, Subsection: HGB Flow Cell Manual Cleaning in the CELL-DYN 1700 Operations Manual).
The Main Amplifier Module (MAM) is located in the main electronics card cage. Alignment of the
WBC, RBC, and PLT gains are critical adjustments that must be verified and/or adjusted before instru-
ment accuracy can be established.
Uniform Latex particles are used to perform these adjustments. The particles must be mixed vigor-
ously before diluting to obtain accurate results.
The Gain and RBC Cell Editing adjustments are performed in the Gain Adjust Mode, which allows
multiple counts to be run on the same sample. When Gain Adjust Mode is entered, whatever is in the
Pre-Mixing Cup is transferred to the WBC Cup and 5 mL of diluent is left undisturbed in the RBC Cup.
WBC Offset
1. Ensure that the instrument is in the READY state.
2. Connect the DVM Pos lead to TP10. Connect the ground to TP9.
3. Adjust R1 (WBC ZERO ADJUST) so that the baseline of the noise signal is positioned at
0.000 volts +/- 0.002.
WBC Gain
1. Prepare a WBC latex dilution.
a. Obtain a clean container. From the MAIN Menu, press [SPECIAL PROTOCOLS] fol-
lowed by [MORE] twice to display the [10 mL DISPENSE] key. Press [10 mL DIS-
PENSE] four times to dispense 20 mL of diluent into the container (must press twice to
dispense 10 mL).
b. Add 1 drop of well mixed 5.0 latex particle solution into the 20 mL of diluent and mix
well.
c. Before entering the Gain Adjust mode, use the WBC latex dilution prepared in step
(b) above to fill the Pre-Mixing Cup up to the level of the diluent inlet port.
NOTE: When the procedure is completed, or if there is a need to exit the Gain Adjust Mode while per-
forming this procedure, such as constant clogs or improper dilution ratio, press [SPECIMEN
TYPE] followed by [PATIENT SPECIMEN].
CELL-DYN® 1700 Service Manual 9140265A-February1995 8-35
Section 8 Search Book TOC Go Back ALIGNMENT AND VERIFICATION
NOTE: The WBC count should be between 10.0 and 30.0. If the count is outside this range, modify
the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
4. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].
5. Press [SMOOTHING OFF]. The key changes to [SMOOTHING ON] and is highlighted,
indicating the smoothing function is ON. Press [WBC HISTOGRAM]. Observe the
lower part of the histogram data to determine the channel number for peak of 100. If the
gain is adjusted properly, the peak count of 100 will be in channel 56 +/- 1 channel.
NOTE: Turning R4 clockwise will increase the channel number; turning R4 counterclockwise will de-
crease the channel number.
6. If the peak count of 100 is not within specification, adjust R4. Return to the RUN screen
and run another cycle. Observe the channel number for peak count of 100. If the gain is
still outside of specification, repeat this process until the peak count of 100 is in channel
56 +/- 1 channel. Refer to Figure 8-9.
7. If only WBC Gain is to be checked, exit the Gain Adjust mode, go to the RUN Menu, and
press [SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
RBC Offset
1. Connect a DVM to TP6 (RBC OUT) and the ground to TP9.
2. Adjust R11 (RBC ZERO ADJUST) so that the baseline of the noise signal is positioned
at 0.00 volts +/- 0.002 volts.
3. Connect a DVM to TP13 (RBC DISCRIMINATOR) and the ground to TP9.
4. Adjust R71 (RBC DISC. ADJUST) for 0.40 volts +/- 0.01 volts.
CELL-DYN® 1700 Service Manual 9140265A-February1995 8-36
Section 8 Search Book TOC Go Back ALIGNMENT AND VERIFICATION
PLT Offset
1. Connect a DVM to TP7 (PLT OUT) and ground to TP9.
2. Adjust R16 (PLT ZERO ADJUST) so that the baseline of the noise signal is positioned at
0.00 volts +/- 0.002 volts.
PLT Gain
1. If in the Gain Adjust mode, prepare a PLT latex dilution by following the steps below:
a. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.
Place a clean container under the Sample Probe and press [10 mL DISPENSE] four
times to dispense 20 mL of diluent (must press twice to dispense 10 mL).
b. Add one (1) drop of well-mixed 3.31 latex particle solution and mix well.
c. Hold the diluted latex solution under the Sample Probe and press [1/50 DILUTION] to
dispense a second dilution.
d. Place a clean container under the Sample Probe and press [1/50 DILUTION] to
aspirate the second dilution.
e. Pull open solenoid 2-5 to drain the existing solution in the RBC Mixing Chamber.
f. Pour the second latex solution into RBC Mixing Chamber.
To minimize problems like coincidence passage, the CELL-DYN 1700 uses two different dilution
ratios of whole blood to diluent. The ratio for WBC/HGB is 1:285; the ratio for RBC/MCV/PLT is
1:12,801. The following procedure will be used to verify the diluent dispense to maintain proper dilu-
tion ratios and thereby optimize instrument performance.
The following procedures provide step-by-step instructions to correctly adjust the positions of
Microswitches 1 through 4 on the probe assembly and to correctly align the Sample Probe height.
If the diluent syringe needs to be replaced, the calibration block must be removed from the old syringe
and placed on the new syringe. Follow the instructions below for removing and installing the calibra-
tion block on the syringe. Figure 8-14 illustrates the diluent syringe.
NOTE: Refer to Section 9, Subsection: Diluent Syringe Cleaning in the CELL-DYN 1700 Opera-
tions Manual for detailed instructions on removing and replacing the diluent syringe.
1. Remove the diluent syringe from the instrument as described in the CELL-DYN 1700
Operations Manual.
2. Remove the calibration block from the old syringe using a 7/64” Allen wrench.
3. Install the new syringe in its luer lock fitting.
4. Slide the calibration block onto the plunger rod of the new syringe, then press [SYRINGE
UP] to move the drive ring up. The calibration block should be resting on top of the ring.
5. Reinstall the front section of the syringe holding clamp. Secure it with the clamp nuts
removed during the syringe removal procedure (refer to step 1). Install the clamp nuts
with the larger hole facing the screw. Tighten the clamp nuts finger-tight with the
beveled edge toward the holding clamp. Do not overtighten.
Perform the following procedure to adjust the Short Sample Sensor on the Closed Sample Assembly:
1. Obtain a new vial of CELL-DYN 16 Low Control.
2. Using CELL-DYN diluent, dilute the Low Control to obtain a reading of 0.8 M/L +/- 0.08
for RBC on the Final Function Test reference instrument.
NOTE: Always use CELL-DYN diluent for sample dilution and always use the diluted solution the same
day. Do not allow the diluted solution to sit overnight.
3. Hook the positive probe of the DVM to TP4 and the negative probe of the DVM to TP3
(ground of the Blood Sensor printed circuit board #9601165 located inside the Closed
Sample Assembly).
4. From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times then
[SERVICE DEC CODE]. The message <ENTER NUMBER (CURRENTLY, XXX):> will
be displayed on the screen.
5. Place a well-mixed VACUTAINER® of sample in the tube holder well (cap facing down)
and snap the tube in place. Type “33” and press Enter.
6. When the flow sequence is completed, adjust R1 and set the voltage of TP4 to 2.5V +/-
0.1V.
7. Measure the voltage of TP1.
8. If the voltage is within the range of 0.3V - 3.25V, press [SERVICE DEC CODE] again.
The message <ENTER NUMBER (CURRENTLY, 33):> will be displayed on the screen.
PLANNED MAINTENANCE
SECTION OVERVIEW
The Planned Maintenance Checklist for the CELL-DYN 1700 is available to the Field Service Repre-
sentative on the lap-top computer assigned to the representative and is also available from the Tech-
nical Services Group.
Future revisions of the Planned Maintenance Checklist will be released as Technical Service Bulle-
tins.
Table of Contents
B.1 SECTION OVERVIEW
B.2 CLOSED SAMPLE DESCRIPTION
Detection of Closed Sample Capability
Detection of Short Sample (Insufficient Aspiration)
Setting Open, Closed, and Pre-Dilute Calibration Factors
Using Count Test Function in the Closed Mode
Auto Clean Function
Clean Sampler Function
B.3 CLOSED SAMPLE MODULE CONFIGURATION
B.4 CLOSED SAMPLE ASSEMBLY FUNCTIONAL SEQUENCE DESCRIPTION
B.5 CLOSED SAMPLE MODULE TROUBLESHOOTING
Service DEC Codes
Motor Power Test (Service DEC Code 128)
Motor Exercise Test (Service DEC Code 130)
List of Figures
Figure 1 Ready Mode
Figure 2 VACUTAINER® Vent
CELL-DYN® 1700 Service Manual 9140265A-February1995 B-1
Appendix B Search Book TOC Go Back CLOSED SAMPLE OPTION
Figure 3 VACUTAINER® Aspiration
Figure 4 Continued VACUTAINER® Aspiration
Figure 5 VACUTAINER® Aspiration Completed
Figure 6 Sample Presentation
Figure 7 Sample Drain
Figure 8 Sample Transfer Cup Soak
Figure 9 Sample Transfer Cup Rinse
Figure 10 Sample Transfer Cup Rinse Complete
Figure 11 System Rinse
Figure 12 Diluent Tubing Air Gap
Figure 13 System Rinse Completed
This appendix is designed to aid the Field Service Representative (FSR) in the troubleshooting and
repair of the CELL-DYN 1700CS Closed Sample assembly. Before attempting any repair of the
Closed Sample assembly, the FSR should verify that the instrument is operating properly in the Open
mode and that the problem is being caused by a malfunction in the Closed Sample assembly.
When a closed VACUTAINER® is inserted (cap facing down) into the VACUTAINER® holder well
and the Touch Plate on the Closed Sample Assembly is pressed, the instrument aspirates 450 µL of
sample. The Closed Sample cycle performs the following four major functions:
1. Diluent residue rinse (from the previous cycle)
2. Sample presentation and aspiration
3. Sample transfer cup soak and rinse
4. Needle waste well rinse
A detailed description of the steps in each of these functions is given in Section B.4.
The Closed Sample Module includes the following major assemblies. Refer to schematic #9480081 in
Section 6.
1. Needle Drive Assembly, Needle Drive Motor, and Stepper Driver printed circuit board
2. Sample Pump Motor and Stepper Driver printed circuit board
3. Diluent Pump Motor and Stepper Driver printed circuit board
4. Sample Transfer Cup
5. Sample Detector
The following is a description of the functions performed by the Closed Sample Assembly during a
sample cycle. The sequence begins when the instrument is in the READY mode and the Touch Plate
is pressed. The sequence ends when the instrument returns to the READY state.
Legend:
The normal solid line for the tubing indicates the Closed Sample Assembly is in the READY state.
The heavy solid line for the tubing indicates the line is filled with liquid during the closed sample cycle.
The dashed line for the tubing indicates the line contains residue during the closed sample cycle.
The CELL-DYN 1700 Instrument System Interface Specification is contained in this section. It is a
separate document published by Abbot Diagnostics. It is included in this manual to assist Field Ser-
vice Representatives in answering questions and resolving problems related to the interface of the
system to an external computer.
SYSTEM INTERFACE SPECIFICATION
Interface Specification
CELL-DYN 1700 SYSTEM
List Number 04H03-01
REVISION A
This document describes the interfacing characteristics of the Abbott CELL-DYN® 1700
automated hematology analyzer when attached to a Host.
The CELL-DYN 1700 provides a standard DB-9 male connector, labeled COM1,
mounted on the side of the instrument.
Pins on the DB-9 connector: (standard RS-232 9 pin assignments)
Pin 1: Data Carrier Detect (DCD input) (monitored)
Pin 2: Data to CELL-DYN 1700 (RD - receive data) (ACK/NAK, XNO/XOFF)
Pin 3: Data to Host (LIS, results) (TD - transmit data)
Pin 4: Data Terminal Ready (DTR) (set true)
Pin 5: Signal Ground
Pin 6: Data Set Ready (DSR) (monitored)
Pin 7: Request to Send (RTS output) (set true)
Pin 8: Clear to Send (CTS input) (monitored, if requested in Setup)
Pin 9: Ring Indicate (RI input) (unused)
3.2 The maximum recommended cable length is 30 meters, or 100 feet. The actual maxi-
mum workable cable length is dependent on the environment of the site, the selected
baud rate, and the equipment being connected together.
4.2 All information transmitted is in character form and is represented by 7-bit ASCII.
4.3 Transmitted characters consist of one (1) start bit, seven (7) or eight (8) data bits (least
significant first), one or no parity bit, and one (1) or two (2) stop bits.
4.5 The transmission speed may be selected from 1200, 2400, 4800, or 9600 bits per sec-
ond (bps).
Transmission control is provided in two ways: 1) XOFF/XON protocol in which the Host
transmits an XOFF character (hex 13) to stop transmission from the System and an
XON character (hex 11) to re-start transmission; and 2) CTS (Clear To Send) hardware
control.
The XOFF/XON protocol has a 1.5 second time-out. If no XON is received for 1.5 sec-
onds after an XOFF, then transmission resumes anyway.
The CTS hardware control has no time-out. That is, no data transmission will be started
with CTS false. However, the transmission will still time-out if at any one time CTS
remains false for longer than the time-out selected in the COMPUTER SETUP Menu
(see Section 5.3.1 below). This is a different use and meaning for time-out, but it is
needed to prevent the Host from indefinitely delaying the CELL-DYN 1700 from pro-
cessing the next sample. Re-transmission requests are also supported and discussed
in Section 5.3.
Numeric data are transmitted in fields of fixed length with zeros used to fill empty
spaces on the left. Out-of-range numeric values are represented by strings of ">"
characters (hex 3E), and undefined numeric values are represented by strings of "-"
characters (hex 2D). Alphanumeric data are transmitted in fields of fixed length
enclosed in double quotation marks (hex 22). Within the quotation marks, the data
are right-justified and blanks (hex 20) are used to fill empty spaces. Fields are sepa-
rated by commas (hex 2C).
6.1 The Identification segment of each message identifies the type of message and the
specimen the message represents.
The Message Type field identifies the message. There are four message types:
WBC Histogram Message - ...................Type "WBC"
RBC Histogram Message- .....................Type "RBC"
PLT Histogram Message - Type "PLT"
Count Data Message - Type " " (3 blanks)
The Instrument Type is an alphanumeric field of seven (7) characters enclosed in dou-
ble quotation marks. The string for the CELL-DYN 1700CS is sent as "CD1700C" and
for the CELL-DYN 1700 as " CD1700".
The Sequence Number is a numeric field of four (4) characters with a value ranging from
O to 4999.
The Specimen Type is the fifth field in the Identification Segment. It occupies a numeric
field of two characters whose value identifies the specimen type as follows:
00: Patient type "PATIENT"
01: Replicate 1 "REPLIC 1"
02: Replicate 2 "REPLIC 2"
03: Replicate 3 "REPLIC 3"
The Operator ID is a alphanumeric field of three (3) digits enclosed in double quotation
marks. If no Operator ID is specified, the field is transmitted as "---".
The Specimen Date, giving the date on which the specimen was run, is an alphanumeric
field of eight (8) characters enclosed in double quotation marks. The default format of
the date is MM/DD/YY, where MM represents the month in two digits, DD represents the
day of the month, and YY represents the year. The date format can be changed in the
DATE/TIME Menu.
The Specimen Time is a alphanumeric field of five (5) characters enclosed in double
quotation marks. It gives the time at which the specimen was run in standard 24-hour
format.
The Specimen Name is the tenth field in the Identification Segment. It occupies a text
field of sixteen (16) characters enclosed in double quotation marks, and it is undefined
except for patient samples.
The Specimen Sex field consists of one (1) character "M" or "F" enclosed in double quo-
tation marks. If not entered by the operator, Specimen Sex is transmitted as a blank " ".
The Specimen Date of Birth field has an identical format to Field 7, except that Field 7 is
automatically generated by the CELL-DYN 1700. If DOB is not entered by the operator,
it is transmitted as "bb/bb/bb" where the "bb"s are actually 2 ASCII blanks each.
The Dr Name field is an alphanumeric field of twenty two (22) characters enclosed in
double quotation marks.
The Collection Date field is an alphanumeric field of five (5) characters enclosed in dou-
ble quotation marks. The format is MM/DD, where MM represents the month in two dig-
its and DD represents the day. The year is not included. It gives the date at which the
specimen was collected. If the Collection Date is not entered, it is transmitted as "bb/bb"
where the "bb"s are 2 ASCII blanks as in Field 12 above.
The Collection Time field is an alphanumeric field of five (5) characters enclosed in dou-
ble quotation marks. It gives the time at which the specimen was collected. If the Collec-
tion Time is not entered, it is transmitted as "bb:bb" ("bb"s are 2 ASCII blanks each and
":" is the middle byte).
The Comment field is an alphanumeric field of sixteen (16) characters enclosed in dou-
ble quotation marks.
Refer to Table 3, Count Data Messages, and Table 4, Count Record Dump.
The parameters reported by the instrument may be represented in any of four different
sets of measurement units as follows:
Set 1 - Standard USA
Set 2 - SI
Set 3 - Modified SI (HGB/MCHC in mmol/L, MCH in fmol)
Set 4 - Modified SI (HCT/PCT in %)
NOTE: The numeric values transmitted to the host are always sent in Standard USA
units even if the unit of measure, also being transmitted, is Set 2, 3, or 4.
Note: Each line contains 16 characters starting with i, not zero. The "02" and "." bolded on the first line
refer to the STX character. The "03" and "65" bolded on the last line refer to the ETX and CRC char-
acters respectively.
9B [checksum]
NOTE: Sample checksum value
may not match this record's data.
[ETX] [end of text]