3.

Analisa kandungan Vitamn E dengan KCKT fase terbalik

 Larutan baku campuran

15 mg α- tokoferol ditambahkan 10 mg δ-tokoferol kemudian ditambahkan γ-
tokoferol 5 mg

 Data Larutan Baku Kerja

A. α- tokoferol 15 mg dilarutkan pda ethanol 50 ml

15 mg 300 mg
= = 300 mg/L (PPM)
50 ml 1000 ml

Konsentrasi larutan baku kerja

10 μl
1. x 300 PPM = 3 PPM
1000 μl
20 μl
2. x 300 PPM = 6 PPM
1000 μl
30 μl
3. x 300 PPM = 9 PPM
1000 μl
40 μl
4. x 300 PPM = 12 PPM
1000 μl
50 μl
5. x 300 PPM = 15 PPM
1000 μl

B. γ- tokoferol 5mg dilarutkan pada methanol 50 ml

5 mg 100 mg
= = 100 mg/L (PPM)
50 ml 1000 ml

Konsentrasi larutan baku kerja

10 μl
1. x 100 PPM = 1 PPM
1000 μl
20 μl
2. x 100 PPM = 2 PPM
1000 μl
30 μl
3. x 100 PPM = 3 PPM
1000 μl
40 μl
4. x 100 PPM = 4 PPM
1000 μl
50 μl
5. x 100 PPM = 5 PPM
1000 μl

C. δ -tokoferol 10 mg dilarutkan dalam methanol 50 ml

10 mg 200 mg
= = 200 mg/L (PPM)
50 ml 1000 ml
 Konsentrasi larutan baku kerja
10 μl
1. x 200 PPM = 2 PPM
1000 μl
20 μl
2. x 200 PPM = 4 PPM
1000 μl
30 μl
3. x 200 PPM = 6 PPM
1000 μl
40 μl
4. x 200 PPM = 8 PPM
1000 μl
50 μl
5. x 200 PPM = 10 PPM
1000 μl

a. Hitungan persamaan regresi

 α – tokoferol
a = 7.3602
b = 35.753 Y= 7.360,2 + 35.753x
r = 0,99993

 γ- tokoferol
a = 3.768
b = 54.4682 Y = 3.768 + 54.4682x
r = 0,99967

 δ -tokoferol
a = 4.531,6
b = 32.8207 Y = 4.531,6 + 32.8207x
r = 0,999929

b. Perhitungan kadar

α – tokoferol

Y= 7.360,2 + 35.753x

1. Area 1
Puncak 1 → 272.675 = 7.360,2 + 35.753x
272.675−7.360,2
X = = 7,420
35.753
Puncak 2 → 190.987 = 7.360,2 + 35.753x
190.987−7.360,2
X = = 5,136
35.753
Puncak 3 → 225.799 = 7.360,2 + 35.753x
225.799−7.360,2
X = = 6,109
35.753
 Puncak 4 → 890.555 = 7.360,2 + 35.753x
890.555−7.360,2
X = = 24,702
35.753
Puncak 5 → 367.889 = 7.360,2 + 35.753x
367,889−7.360,2
X = = 10,084
35.753

2. Area 2
Puncak 1 → 285.666 = 7.360,2 + 35.753x
285.666−7.360,2
X = = 7,784
35.753
Puncak 2 → 205.666 = 7.360,2 + 35.753x
205.666−7.360,2
X = = 5,546
35.753
Puncak 3 → 235.578 = 7.360,2 + 35.753x
235.578−7.360,2
X = = 6,383
35.753
Puncak 4 → 975.777 = 7.360,2 + 35.753x
975.777−7.360,2
X = = 27,086
35.753
Puncak 5 → 477.932 = 7.360,2 + 35.753x
477.932−7.360,2
X = = 13,162
35.753

3. Area 3
Puncak 1 → 317.268 = 7.360,2 + 35.753x
317.268−7.360,2
X = = 8,668
35.753
Puncak 2 → 250.796 = 7.360,2 + 35.753x
250.796−7.360,2
X = = 6,808
35.753
Puncak 3 → 304.778 = 7.360,2 + 35.753x
304.778−7.360,2
X = = 8,318
35.753
Puncak 4 → 1.076.322= 7.360,2 + 35.753x
1.076.322−7.360,2
X = = 29.898
35.753
Puncak 5 → 589.134 = 7.360,2 + 35.753x
589.134−7.360,2
X = = 16,272
35.753

γ- tokoferol
Y = 3.768 + 54.4682x
1. Area 1
Puncak 1 → 272.675 = 3.768 + 54.4682x
272.675−3.768
X = = 4,937
54.468,2
Puncak 2 → 190.987 = 3.768 + 54.4682x
190.987−3.768
X = = 3,437
54.468,2
Puncak 3 → 225.799 = 3.768 + 54.4682x
225.799−3.768
X = = 4,076
54.468,2
Puncak 4 → 890.555 = 3.768 + 54.4682x
890.555−3.768
X = = 16,280
54.468,2
Puncak 5 → 367.889 = 3.768 + 54.4682x
367,889−3.768
X = = 6,685
54.468,2

2. Area 2
Puncak 1 → 285.666 = 3,768 + 54.468,2x
285.666−3,768
X = = 5,175
54.468 ,2
Puncak 2 → 205.666 = 3,768 + 54.4682x
205.666−3,768
X = = 3,705
54.468 ,2
Puncak 3 → 235.578 = 3,768 + 54.4682x
235.578−3,768
X = = 4,256
54.468 ,2
Puncak 4 → 975.777 = 3,768 + 54.4682x
975.777−3,768
X = = 17,845
54.468 ,2
Puncak 5 → 477.932 = 3,768 + 54.4682x
477.932−3,768
X = = 8,705
54.468 ,2

3. Area 3
Puncak 1 → 317.268 = 3,768 + 54.468,2x
317.268−3,768
X = = 5,755
54.468 ,2
Puncak 2 → 250.796 = 3,768 + 54.468,2x
250.796−3,768
X = = 4,535
54.468 ,2
Puncak 3 → 304.778 = 3,768 + 54.468,2x
304.778−3,768
X = = 5,526
54.468 ,2
Puncak 4 → 1.076.322= 3,768 + 54.468,2x
 1.076.322−3,768
X = = 19,691
54.468 , 2
Puncak 5 → 589.134 = 3,768 + 54.468,2x
589,134−3,768
X = = 10,747
54.468 ,2

δ -tokoferol
Y = 4.531,6 + 32.820,7x
1. Area 1
Puncak 1 → 272.675 = 4.531,6 + 32.8207x
272.675−4.531,6
X = = 8,169
32.820 ,7
Puncak 2 → 190.987 = 4.531,6 + 32.8207x
190.987−4.531,6
X = = 5,681
32.8207
Puncak 3 → 225.799 = 4.531,6 + 32.8207x
225.799−4.531,6
X = = 6,741
32.8207
Puncak 4 → 890.555 = 4.531,6 + 32.8207x
890.555−4.531,6
X = = 26,995
32.8207
Puncak 5 → 367.889 = 4.531,6 + 32.8207x
367.889−4.531,6
X = = 11,070
32.8207

2. Area 2
Puncak 1 → 285.666 = 4.531,6 + 32.620,7x
285.666−4.531,6
X = = 8,566
32.8207
Puncak 2 → 205.666 = 4.531,6 + 32.820,7x
205.666−4.531,6
X = = 6,128
32.8207
Puncak 3 → 235.578 = 4.531,6 + 32.820,7x
235.578−4.531,6
X = = 7,039
32.8207
Puncak 4 → 975.777 = 4.531,6 + 32.820,7x
975.777−4.531,6
X = = 29,592
32.8207
Puncak 5 → 477.932 = 4.531,6 + 32.820,7x
477.932−4.531,6
X = = 14,424
32.8207
3. Area 3
Puncak 1 → 317.268 = 4.531,6 + 32.8207x
317.268−4.531,6
X = = 9,528
32.8207
Puncak 2 → 250.796 = 4.531,6 + 32.8207x
250.796−4.531,6
X = = 7,503
32.8207
Puncak 3 → 304.778 = 4.531,6 + 32.8207x
304.778−4.531,6
X = = 9,148
32.8207
Puncak 4 → 1.076.322= 4.531,6 + 32.8207x
1.076.322−4.531,6
X = = 32,656
32.8207
Puncak 5 → 589.134 = 4.531,6 + 32.8207x
589.134−4.531,6
X = = 17,812
32.8207