Calibrations

6. Calibrations

Contents
Calibrations
Technical Facilities ..................................... 6:2
Before calibration ........................................ 6:2

A/D Converters
A/D Converters ........................................... 6:3

Transducers
Calibration of linear transducers ............... 6:4
Selection diagram .................................. 6:4
Venous Pressure Transducer Calibr ...... 6:5
Arterial Pressure Transducer Calibr .... 6:6
Priming Detector calibration ................. 6:7
Occlusion adjustment, blood pump ....... 6:7
Blood Leak Detector calibration ............ 6:8
Conductivity Transducer calibration .... 6:9
Dialysis Pressure Transd Calibr ......... 6:10
Degassing Pressure Transd Calibr ...... 6:11
pH transducer calibration ................... 6:12
Pressure Regulator Calibr, PR 1 ......... 6:14
Pressure Regulator Calibr, PR 2 ......... 6:14
HPG Pressure Transducer Calibr ........ 6:15
Variable flow calibration ...................... 6:16

UF Calibration ....................................... 6:17

General ................................................. 6:17
Complete .............................................. 6:18
Intern .................................................... 6:19
Verify .................................................... 6:20

BPM test .................................................. 6:21

AK 95 / AK 95 S Service Manual 6:1

HCEN 9138 Rev. 10.2005
Calibrations

Calibration AK 95 S
Warning! After completing the calibrations, the conductivity value must always be confirmed by the
hospital laboratory.

Technical Facilities Before calibration

The following instructions are based on the
To get information about the use of the
availability of some technical facilities, listed
control buttons, read chapter 4; Service
below.
Technicians Guide.

Ordering no:
UF - calibration:
UF-calibration box K21908002

AD:
Digital voltmeter

Priming detector:
A piece of blood tube used at the hospital.

Blood leak detector

Calibration cover K40169001

Pressure
Reference instrument 300333001
Calibration kit K13983002

Special tool
For silicone tube connectors K12755001

Calibration kit

6:2 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

AD Converters
1. Start the AK 95 S, enter the Service menu 5. Set the “AD REF VOLTAGE” on the
and select Calibrations. display to the value shown on the voltme-
ter and press .
2. Choose AD-converter.

When selecting “AD-Converter” from the display

START
6. Press UF to load the value into the
with the SET knob and press key following STOP

display appears: EEPROM.

SELECT AD-CONVERTER
CPU X AD-REF VOLTAGE STORED
CPU-C CPU-P CPU-W MM95

3. Select an AD-converter with the SET knob

7. Press to exit.
and press key.

CPU X AD LOW REF SET HIGH

xx.xx mV xx.xx xx.xx xx.xx

4. Measure the voltage (using a 4.5 digit

voltmeter), between following testpoints or
across the resistor:

CPU Testpoint Ref Tetspoint Circuit board Limits (mV)

CPU-C TP 6 TP 5 Control Driver board 45.00 - 55.00

CPU-P TP 1 TP 2 Protective Driver board 45.00 - 55.00
CPU-W TP 3 TP 4 Protective CPU board 4500 - 5500
MM 95 R 24 R 24 MM I/O board 45.00 - 55.00

CPU C driver board AD ref voltage range:

45.00 to 55.00 mV
AD ref voltage range:
45.00 to 55.00 mV PVREF
TP1

TP 5 TP2
Z0VAD
CPU C A/D ref

TP 6 Buzzer

AD ref voltage range:

4.500 to 5.500 V

TP3 MM I/O AD ref voltage range:

WREF 45.00 to 55.00 mV

(TP1)
CPU W PROM (TP2)
TP4 R24
Z0VRW
CPU W
SafeRAM
pH plug

AK 95 / AK 95 S Service Manual 6:3

HCEN 9138 Rev. 10.2005
Calibrations

Transducers
Calibration of linear
transducers
1. Start the AK 95 S, enter the Service • Select Internal and the AK 95 S main-
menu and select Calibrations. tains a flow during the calibration.

2. Choose Transducers Independently wich flow support is chosen

the Selection diagram turns up.
3. Follow the instructions for each
calibration. 4. When turning the SET knob all the
different transducers that are possible to
• Select Extern for no flow calibrate will “scroll” on the Information
(pumps stopped). display.

Selection diagram

VENOUS PRESSURE TRANSDUCER CPU P Run Time Meter

VENOUS PRESSURE TRANSDUCER CPU C pH TRANSDUCER

ARTERIAL PRESSURE TRANSDUCER HPG PRESSURE TRANSDUCER

PRIMING DETECTOR DEGASSING PRESSURE TRANSDUCER

BLOOD LEAK DETECTOR DIALYSIS PRESSURE TRANSDUCER

CONDUCTIVITY A TRANSDUCER CONDUCTIVITY A B P TRANSDUCERS

CONDUCTIVITY B TRANSDUCER CONDUCTIVITY P TRANSDUCER

6:4 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

Venous Pressure Transducer Calibration VEN PR LOW REF SET HIGH

1.2 -550 +500 550
1. Connect the Gambro reference instrument
(together with a calibration tube set) to 11. Set the “REF SET” value on the AK 95 S
the venous pressure transducer on the to the reading on the reference instrument
front of the AK 95 S, see fig. below.
and press .
2. Zeroset the reference instrument, wait for
stable reading. 12. Press
START
UF to load the calibration values
STOP

3. Start the AK 95 S, enter the Service into the EEPROM.

menu and select Calibrations.
13. When the EEPROM storage is ready,
4. Select Transducers, External and press to exit.
VENOUS PRESSURE TRANSDUCER
CPU P from the selection diagram. Continue with the venous pressure transducer
calibration for CPU C.
5. Press to confirm the selected trans- Follow this instruction except for paragraph 4,
where VENOUS PRESSURE TRANSDUCER
ducer.
CPU C shall be selected instead of CPU P.
6. Apply zero pressure by opening the three
If a calibration error occurs:
clamps A, B and C on the calibration tube
set. Set the “REF SET” value on the
AK 95 S to 0 mmHg. • Try to do the calibration again.

• If the error occurs a second time:

7. Press to enter the setting. Change the transducer and the transducer
board.
START Report to your Gambro service representa-
8. If the lit UF key is pressed at this time
STOP
tive the error message on the error dis-
the machine stores the offset for the play.
transducer in the EEPROM and updates
the checksum.

For the sensitivity calibration

(the slope):
Close clamp A. Apply a pressure of approx.
-500 mmHg, shown on the reference in-
strument. Close clamp B.

VEN PR LOW REF SET HIGH

1.2 -550 +500 550

9. Set the “REF SET” value on the AK 95 S

to the reading on the reference instrument
and press twice.

10. Open clamp A and B, then close clamp A

again. Apply a pressure of approx. +500 C
mmHg, shown on the reference instru-
ment. Close clamp B. B

AK 95 / AK 95 S Service Manual 6:5

HCEN 9138 Rev. 10.2005
Calibrations

Arterial Pressure Transducer Calibration

1. Connect the Gambro reference instrument ART PR LOW REF SET HIGH
(together with a calibration tube set) to 1.2 -550 +500 550
the arterial pressure transducer on the
front of the AK 95 S, see fig. below. 11. Set the “REF SET” value on the AK 95 S
to the reading on the reference instrument
2. Zeroset the reference instrument, wait for
stable reading. and press .

3. Start the AK 95 S, enter the Service START

12. Press UF to load the calibration values
menu and select Calibrations. STOP

into the EEPROM.

4. Select Transducers, External and
ARTERIAL PRESSURE TRANSDUCER 13. When the EEPROM storage is ready,
from the selection diagram. press to exit.

5. Press to confirm the selected trans-

ducer.
If a calibration error occurs:
6. Apply zero pressure by opening the three
clamps A, B and C on the calibration tube • Try to do the calibration again.
set. Set the “REF SET” value on the
AK 95 to 0 mmHg. • If the error occurs a second time:
Change the transducer and the transducer
board.
7. Press to enter the setting. Report to your Gambro service representa-
tive the error message on the error dis-
8. If the lit
START
UF key is pressed at this time play.
STOP

the machine stores the offset for the

transducer in the EEPROM and updates
the checksum.

For the sensitivity calibration

(the slope):
Close clamp A. Apply a pressure of approx.
-500 mmHg, shown on the reference in-
strument. Close clamp B.

ART PR LOW REF SET HIGH

1.2 -550 -500 550

9. Set the “REF SET” value on the AK 95 S

to the reading on the reference instrument
and press twice.
C
10. Open clamp A and B, then close clamp A
again. Apply a pressure of approx. +500 B
mmHg, shown on the reference instru-
ment. Close clamp B.

6:6 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

Priming Detector calibration Occlusion adjustment, blood pump/

1. Start the AK 95 S, enter the Service infusion pump
menu and select Calibrations.
Check surface on the roller at the pump rotor, if
2. Select Transducer, External and it is damaged exchange the unit. The roller unit
PRIMING DETECTOR from the selection should easily go back to upper end position when
diagram. it has been pressed down to end position, ex-
change unit if it gets stuck in any position.
3. Press to start calibration routine.
Note! If the blood lines/infusion lines have a
different wall thickness, the occlusion of the
4. The display shows: blood pump must be adjusted to the right size.

INSERT TUBE Use the gauge pins (stop/go) available in the kit
Press SELECT to confirm K40158001. The kit includes following three
different gauge pins:
5. Insert an empty tube in the priming
T = 1.1 (Stop pin = 1.1*2*0.7 = 1.5mm)
detector and press .
T = 1.6 (Stop pin = 1.6*2*0.7 = 2.2mm)
Use the normal tube from the blood line. T = 2.0 (Stop pin = 2.0*2*0.7 = 2.8mm)

6. The calibration is now done automatically T = wall thickness of the blood tubing
and the calibration factor is writen into
the EEPROM. Use following formula to calculate which gage
pin to use:
7. When the info. display shows “EEPROM 2 * wall thickness * 0,7 (mm)
STORAGE COMPLETED”, the calibration
is completed.

8. Press to exit.

If an technical error is detected, the calibration

must be restarted, or the priming detector
Check the range between the blood pump rollers
replaced.
and the path according to the picture below.
Adjust range between the blood pump rollers and
the path if it is necessary.

The GO pin should barely pass within the cali-

brating area.

The STOP pin is NOT allowed to pass in any

point within the calibrating area.

Note! Put grease on the GO and STOP pins

when not used and store gage in plastic bag to
prevent it from corroding.

= Measuring

AK 95 / AK 95 S Service Manual 6:7

HCEN 9138 Rev. 10.2005
Calibrations

Blood Leak Detector calibration

1. Make sure there is no fluid in the AK 95 S
Monitor. If fluid in the monitor, press
Rinse/Drain.
2. Remove the blood leak detector cover and
clean the inside of the blood leak housing.
3. Mount a blood leak calibration cover
(K40169001), make sure that the filter is in
horisontal position .
Start the AK 95 S, wait until buzzertest is
done and bargraphs are lit.
If a calibration error occurs:
• Check the position of the calibration filter
and repeat from point 1.
• If an error occurs after the 2nd try, change
the blood leak detector.
• After calibration, clean the filter with
water.
Replace the filter when it has been used
for more than 10 times.

4. Start the AK 95 S, enter the Service

menu and select Calibrations.

5. Select Transducer, Intern and

BLOOD LEAK DETECTOR from the
selection diagram.

6. Place A-pickup tube in the acetate-

concentrate container.

7. Press to enter the Blood leak calibra-

tion routine.
Let the machine stabilize in conductivity
and temperature (37.5 °C) a few minutes.
Wait for green fluid path.

8. Make sure the filter is in horizontal

position and set “REF SET” to 0.

9. Press when the BL LEAK value is

stable.

10. Turn the calibration filter to vertical

position and set “REF SET” to 100.

11. To continue, press twice when the

BL LEAK value is stable.

START
12. Press UF
STOP
to store the calibration values.

13. Wait until the display shows “EEPROM

STORAGE COMPLETED”.

14. Press to exit.

15. Drain the AK 95 S and remount the

standard blood leak cover.

6:8 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

Conductivity Transducer calibration

The calibration is a “two point calibration”. The 5. Place A-pickup tube in the concentrate
two points are 0.0 mS/cm (RO-water) and container. The AK 95 S will now regulate
14.0 mS/cm (with concentrate). conductivity to approx. 14 mS/cm. The
green fluid path must be lit.
NOTE!
Make sure the +500ml/min restrictor is mounted 6. Let the conductivity stabilize for 20 min-
before calibration. utes. (green fluid path)
Press “TEMP” to check that temperature
NOTE! is 37,5°C.
• Before conductivity calibration, Chemical Press to get back the conductivity
cleaning must be performed, one with Citric acid
calibration display.
and one with Hypochlorite.
7. Set the “REF. SET” value on the AK 95 S
1. Enter the Service menu, Calib, Transd
display to the same value shown on the
and select Intern.
reference instrument.
Set the display at the conductivity trans-
ducer to be calibrated.
The possible cond. cell calibrations are: 8. Press to enter the value.
• CONDUCTIVITY A TRANSDUCER
• CONDUCTIVITY B TRANSDUCER 9. The machine will now ask you to confirm
• CONDUCTIVITY P TRANSDUCER to enter sensitivity calibration.
• CONDUCTIVITY ABP TRANSDUCERS
Press to confirm.
2. Connect the Gambro reference instrument
to the dialyzer connectors. To exit: press (will stop calibration).
Connect both the A- and B-pickup tubes to
the MM 95. START
10. Press UF
STOP
to load the calibration values
Set the temperature to 37,5°C (by pressing into the EEPROM.
TEMP key) and let the AK 95 S and the refer-
ence instrument stabilize for at least 30 min- All the transducers A, B and P are all calibrated
utes. in this way.

Make sure that the machine is not in bypass, i.e.

the fluid bypass button is not lit. There must
be flow through the measuring cell.

3. Press to enter the calibration routine.

4. Wait until the reading on the reference

instrument has stabilized at 0.0 mS/cm.
Set REF SET to 00.0 with Set knob.

Press “TEMP” and check that the tem-

perature is 37,5°C.
Press to get back the conductivity
calibration display.
Press to enter the value.

AK 95 / AK 95 S Service Manual 6:9

HCEN 9138 Rev. 10.2005
Calibrations

Dialysis Pressure Transducer Calibration

START
1. Disconnect tube no. 46, 20 and the silicone 14. Press UF
STOP
to load the calibration values
tube at the deairating chamber see fig. into the EEPROM.
below.
15. When the EEPROM storage is ready,
2. Plug the two lower silicone connectors at
the deairating chamber. Use two plugs press to exit.
from the calibration kit, see fig. below.

3. Connect the Gambro reference instrument If a calibration error occurs:

to the silicone connector on the top of the
deairating chamber via the calibration
tube set. See fig. below.
4. Zeroset the reference instrument, wait for
stable reading.
5. Start the AK 95 S, enter the Service
menu and select Calibrations.
• Try to do the calibration again.
• If the error occurs a second time:
Change the transducer and the transducer
board.
Report to your Gambro service representa-
tive the error message on the error dis-
play.

6. Select Transducer, External and

DIALYS PRESSURE TRANSDUCER
from the selection diagram.

7. Press to confirm the selected trans-

ducer.

8. Apply zero pressure by opening the two

clamps A, and B on the calibration tube
set. Set the “REF SET” value on the
AK 95 S to 0 mmHg.

9. Press to enter the setting.

10. Close clamp A. Apply a pressure of approx.

-500 mmHg, shown on the reference
instrument. Close clamp B.

11. Set the “REF SET” value on the AK 95 S

to the reading on the reference instrument
and press twice.

12. Open clamp A and B, then close clamp A

again.
Apply a pressure of approx. +500 mmHg,
shown on the reference instrument. Close
clamp B.

13. Set the “REF SET” value on the AK 95 S

to the reading of the reference instrument
and press .

6:10 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

Degassing Pressure Transducer Calibration

1. Disconnect tube no. 50 at the Expansion 13. Set the “REF SET” value on the AK 95 S to
chamber. Connect the Gambro reference the reading on the reference instrument
instrument to tube no. 50 via the calibra-
and press .
tion tube set. Use the angled-silicone
connector from the calibration kit.
START
14. Press to load the calibration values
UF
STOP
2. Disconnect tube no. 11 at the degassing
into the EEPROM.
chamber or at the outlet of the flow pump
and plug the silicone connector. Use a plug
15. When the EEPROM storage is ready,
from the calibration kit.
press to exit.
4. Zeroset the reference instrument, wait for
stable reading.
If a calibration error occurs:
5. Start the AK 95 S, enter the Service
menu and select Calibrations. • Try to do the calibration again.

6. Select Transducer, External and • If the error occurs a second time:

DEGASSING PRESSURE TRANSDUCER Change the transducer and the transducer
from the selection diagram. board.
Report to your Gambro service representa-
7. Press to confirm the selected trans- tive the error message on the error dis-
play.
ducer.

8. Apply zero pressure by opening the two

clamps A and B on the calibration tube to tube 50
set. Set the “REF SET” value on the
AK 95 S to 0 mmHg.

9. Press to enter the setting.

10. Close clamp A. Apply a pressure of approx.

-500 mmHg, shown on the reference
instrument. Close clamp B.

11. Set the “REF SET” value on the AK 95 S

to the reading o the reference instrument
and press twice .

12. Open clamp A and B, then close clamp A

again.
Apply a pressure of approx. +500 mmHg,
shown on the reference instrument. Close
clamp B.

AK 95 / AK 95 S Service Manual 6:11

HCEN 9138 Rev. 10.2005
Calibrations

pH transducer calibration

Calibration should be performed on a regular 7. Put the pH sensor in a cup with pH 7

basis to ensure optimum performance. buffer solution (ordering no. 100 810 001)
which must cover the glass membrane i.e.
A complete calibration procedure consists the level in the cup should be at least
of the following steps: 30mm from the bottom.

1. Offset calibration, with a buffer solution 8. Stir in order to achieve balance in the
(a solution with a very accurate and stable buffer solution. Do not let the pH sensor
pH-value) having a pH-value in the range lean against the bottom of the cup.
of pH 6.5-7.5, followed by: Wait for 2 minutes.

2. Slope calibration with a buffer solution 9.* Press to set the “REF SET” value on
having pH-value that differs at least 2 pH-
the AK 95 S and wait until the calibration
units from the buffer used for offset cali-
bration. display returns.

START
Calibration schedule: 10. Press UF
STOP
to load the calibration value
into the EEPROM.
An Offset calibration should be per-
formed after one month in operation and 11. Clean the pH-sensor in RO-water.
then every third month to verify the Remount the pH sensor into the
performance. pH sensorhousing.

A Complete calibration procedure

should be performed at installation and
then every 12 months.

Offset Calibration:

1. Make sure there is no flow or fluid in the

AK 95 S. The inlet water must be closed.

2. Start the AK 95 S, enter the Service

menu and select Calibrations.

3. Select Transducer, External and

pH TRANSDUCER from the selection
diagram.

4. Press to start calibration routine.

5. Remove the pH sensor from the pH sensor

housing by loosening the nut holding the
pH sensor and rinse the sensor in distilled
water.

6. Check that the “REF SET” on the AK 95 S

displays pH 7.

6:12 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

Complete calibration:

1. Make sure there is no flow or fluid in the 13. Stir in order to achieve balance in the
AK 95 S. The inlet water must be closed. buffer solution. The pH sensor must not
lean against the bottom of the cup.
2. Start the AK 95 S, enter the Service Wait for 2 minutes.
menu and select Calibrations.
14.* Press and wait.
3. Select Transducer, External and
pH TRANSDUCER from the selection The display shows: Entering sensitivity
diagram. calibration.

4. Press to start calibration routine. 15. Press to confirm that the calibration
shall continue.
5. Remove the pH sensor from the pH sensor
housing by loosening the nut holding the 16. When the calibration display returns,
pH sensor and rinse the sensor in distilled press
START
UF
STOP
to load the calibration values
water.
into the EEPROM.
6. Check that the “REF SET” on the AK 95 S
17. Remount the pH-sensor into the pH sensor
displays pH 7.
housing.
7. Put the pH sensor in a cup with pH 7
* If an error message is shown after this
buffer solution (ordering no. 100 810 001),
step, check the buffer solutions, level in
or whatever reference solution available,
the cup and repeat the procedure from
which must cover the glass membrane i.e.
step 1. If there still is an error after 2nd
the level in the cup should be at least
try, replace the pH-sensor.
30mm from the bottom.
NOTE! The pH-sensor should be changed every
8. Stir in order to achieve balance in the
eighteen month.
buffer solution. Do not let the pH sensor
lean against the bottom of the cup.
Wait for 2 minutes.

9.* Press to set the “REF SET” value on

the AK 95 S and wait until the calibration
display returns.

10. Rinse the pH sensor in distilled water.

11. Check that the “REF SET” on the AK 95 S

displays pH 9.

12. Put the pH sensor in a cup with pH 9

buffer solution (ordering no. 100810002)
which must cover the glass membrane i.e.
the level in the cup should be at least
30mm from the bottom.

AK 95 / AK 95 S Service Manual 6:13

HCEN 9138 Rev. 10.2005
Calibrations

Pressure Regulator Calibration, PR 1 Pressure Regulator Calibration, PR 2

1. Connect a pressure gauge just before the 1. Disconnect tube no. 4 from the T-connector
Parker connector inside MM 95 according at INVA.
to this picture.

PR 1

2. Install a T-connector in series with the prior.

Connect tube no.4 and the reference instru-
ment to it. Use nipple K0 1515 002 to the
reference instrument connection.
2. Start the AK 95 S.
3. Zeroset the Reference instrument, wait for
3. Perform a functional check and wait until stable reading.
the conductivity and the temperature are
stable. The fluid path on the OP-panel 4. Start the AK 95 S.
must be green before the calibration
begins. 5. Perform a functional check and wait until
the conductivity and the temperature are
4. Adjust the pressure regulator, PR 1, until stable. The fluid path on the OP-panel must
the pressure measured at the pressure be green before the calibration begins.
gauge is 600 mmHg ±75 mmHg.
6. Adjust the pressure regulator, PR 2, until
5. Perform a Drain. the pressure measured at the reference
instrument is 130 mmHg ± 10 mmHg.
6. Disconnect the pressure gauge.
7. Perform a Drain.

8. Reassemble to the normal silicone

T-connector.

Fluid connector
detection

Safety by-pass SAGS

P
Unit
REVA Treg
INPS P Heater T

Water
Inlet Pressure PR 2 INVA
regulator Flow
PR 1 °C switch
AIVA

Heat Suction
exchanger Pump
Drain

Outlet
Restrictor

AK 95 S Inlet/Outlet Flow Diagram. The complete

Flow Diagram is showed at page 2:5.

6:14 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

HPG Pressure Transducer Calibration

1. Disconnect tube no. 23909 at the HPG 15. When the EEPROM storage is ready,
pressure transducer, see fig. below. press to exit.

2. Plug the left side of the HPG pressure

transducer. Use a plug and a stright silicon
If a calibration error occurs:
connector from the calibration kit.
• Try to do the calibration again.
3. Connect the Gambro reference instrument
to the right side of HPG pressure trans-
• If the error occurs a second time:
ducer via the calibration tube set.
Change the transducer and the transducer
board.
4. Zeroset the reference instrument, wait for
Report to your Gambro service representa-
stable reading.
tive the error message on the error dis-
play.
5. Start the AK 95 S, enter the Service
menu and select Calibrations.

6. Select Transducer, External and

HPG PRESSURE TRANSDUCER
from the selection diagram.

7. Press to confirm the selected trans-

ducer.

8. Apply zero pressure by opening the two

clamps A and B on the calibration tube 1
set. Set the “REF SET” value on the 2
AK 95 S to 0 mmHg.

9. Press to enter the setting.

10. Close clamp A. Apply a pressure of approx.

-500 mmHg, shown on the reference
instrument. Close clamp B.

11. Set the “REF SET” value on the AK 95 S

to the reading on the reference instrument
and press twice.

12. Open clamp A and B, then close clamp A

again. Apply a pressure of approx. +500
mmHg, shown on the reference instru-
ment. Close clamp B.

13. Set the “REF SET” value on the AK 95 S

to the reading of the reference instrument
and press .
START
14. Press to load the calibration values
UF
STOP

into the EEPROM.

AK 95 / AK 95 S Service Manual 6:15

HCEN 9138 Rev. 10.2005
Calibrations

Variable flow calibration

This calibration will set the number of steps

needed to obtain a flow of 500 ml/min.
This value will then be used during functional
check and when entering service mode as the
flow always should be 500 ml/min in functional
check and service mode.

1. Start the AK 95 S, enter the Service menu

and select Calibrations.

2. Place A-pickup tube in the concentrate

container.

3. Select Other / Variable flow to initiate the

calibration.

Note: If variable flow is not installed following

attention will be displayed:

VARIABLE FLOW IS NOT INSTALLED

Check preset

4. Following text will be displayed during the

calibration:

STEPS: XXX FLOW: ---- ml/min

Please wait. Press HOLD to abort

STEPS: the position of the stepper motor

FLOW: the average dialysis flow

5. When the AK 95 S machine has found a

new calibration value it will automatically
store the value in the Safe-RAM.
Following text will automatically appear:

ADJUSTING VARIABLE DIALYSIS FLOW

Please wait XX seconds

6. At the end of the calibration the new

calibration data is displayed:

VAR. FLOW MOTOR POSITION STORED

NEW VALUE: XXX OLD VALUE: YYY

7. Press HOLD to finish the calibration.

Note: During this calibration it is possible to

supervise the conductivity and temperature.

6:16 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005

UF Calibration
General
This section describes the calibration routine of
the UF-measuring unit.
The UF-measuring unit consists of two flow
measurement channels called channel 1 (CH 1)
and (CH 2). CH 1 is the channel used to measure
the main flow to the dialyser and CH 2 is used to
measure the flow from the dialyzer, i.e. the sum
of the main flow and the UF flow. The UF flow is
the difference between CH1 and CH2 flows. UF
flow is also called differential flow.
Perform both a disinfection and a cleaning
decalcification to remove both organic and
calcium precipitates, according to the AK 95 S
Operator’s Manual.
The calibration routine can be run in 3 different
modes. The modes are Complete, Intern and
Verify.
- Select Complete for an automatic UF
calibration.
- Select Intern for an internal calibration
(taration but updating the EEPROM instead of
RAM). This is done without the external UF
calibration equipment connected to the
AK 95 S.
It is not possible to select Intern if not a
complete UF calibration has been done on the
UF cell before.
- Select Verify for a verification of the result
from the latest calibration.
High Level
V.ref.
Low Level
Flow in/out
UFVA
Fig. The AK 95 S UF calibration system.
AK 95 / AK 95 S Service Manual
HCEN 9138 Rev. 10.2005
Calibrations
The Complete calibration routine is done with
the mainflow 500 ml/min. It requires that the
external UF calibration equipment is connected
to the AK 95 S, see fig.below. The equipment
contains a reference volume, a flow directing
valve (UFVA) and a UF- calibration box. The
UF calibration box controls the directing valve
by measuring low level and high level of the
reference volume and depending on the selected
UF box mode the valve will become closed or
opened when low or high level is detected.
UF calibration equipment, ordering no.
K2 1908 002
Note! Tubes used to connect the reference
volume to the monitor should not be longer than
standard tubes used for connection of dialysers.
The connection tubes should be routed so that
“air traps” are avoided.
Note!
Make sure the +500ml/min restrictor is mounted
before calibration.
AK
AK9590
S
UF
Cal.
Box
6:17
Calibrations

Complete

Turn on the equipment on the following During the UF-calibration:

order:
1. Plug in the UF calibration box.
Note! All electrical connections must be done
before the power for AK 95 S is turned on.
Be aware of that there is no main switch on the
UF calibration box.
Connect the UF calibration box to the
AK 95 S.
Use the UF calibration cable, K1 4036, to
connect the AK 95 S with the UF calibration
box. The part of the cable that is labeled UF
BOX should be connected to the UF calibration
box.
Note! The complete calibration will not run
(but nothing will get destroyed) if the cable is
reversed.
2. Start the AK 95 S with the main switch and
the ON/OFF button.
The time display shows the time since the
calibration was started.
The information displays also shows the different
stages during the calibration:
• Fluid preparation
• UF stabilization
• Main flow offset calibration
• CH 1 flow calibration
• Differential flow calibration
• Verifying calibration of UF
• Verifying calibration of Isolated UF
When the automatic UF calibration is
ready:
After the calibration AK 95 S continues to store
the calculated values. When the storage is
completed, the verification results are displayed.
First the display for UF Volume for 3 seconds:
UF VOL REF VOL VOL DIFF
1006.5 1007.3 -0.28 %

3. Place B-pickup tube in the acetate-concentrate Then the display for Isolated UF Volume for 3
container. seconds:

4. Enter Service mode. ISO VOL REF VOL VOL DIFF

1007.5 1007.3 +0.28 %
5. Connect the fluid tubes to the UF calibration
box.
Then the previous disply is showed again for
3 seconds and so on.
6. Select Calibration.

7. Select Transducers. When ready, press to exit the Complete UF

calibration.
8. Select UF.
Note! Perform a Rinse before leaving the
9. Select Complete. AK 95 S.

10. Set Reference volume with the SET knob. The equipment is turned off in the
(The Referense volume is labeled on the UF- following order:
Calibration unit) 1 AK 95 S ON/OFF button.
2 AK 95 S main switch.
11. Press to confirm. 3 UF calibration box.

After the Reference volume is confirmed the UF

calibration runs automatically, until the
calibration is finished.

6:18 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

Intern

1. Start the AK 95 S with the main switch and When the internal UF calibration is ready:
the ON/OFF button. After the calibration, AK 95 S displays:

2. Place B-pickup tube in the acetate-concentrate INTERNAL UF CALIBRATION READY

container.
SELECT for info, HOLD to save
3. Enter Service mode.

4. Select Calibration. Press to show more information.

5. Select Transducers.
Press to save the calibrations values.
6. Select UF.
When the storage is completed, AK 95 S displays:
7. Select Intern.
EEPROM STORAGE COMPLETED
The internal UF calibration runs automatically,
until the calibration is finished.
Press HOLD to exit

When ready, press to exit the internal UF

calibration.

Note! If the machine is equipped with a UFDkit,

change preset (1-26 UFD) to ”not inst” before
entering calibration mode.

AK 95 / AK 95 S Service Manual 6:19

HCEN 9138 Rev. 10.2005
Calibrations

Verify

Turn on the equipment on the following
order:
1. Plug in the UF calibration box.
Note! All electrical connections must be done
before the power for AK 95 S is turned on.
Be aware of that there is no main switch on the
UF calibration box.
The information displays also shows the different
stages during the calibration:
• Fluid preparation
• UF stabilization
• Main flow offset calibration
• CH 1 flow calibration
• Differential flow calibration
• Verifying calibration of UF
• Verifying calibration of Isolated UF

Connect the UF calibration box to the When the verification of the UF cell
AK 95 S. calibration is ready:
When the verification is completed, AK95
Use the UF calibration cable, K1 4036, to displays the results:
connect the AK 95S with the UF calibration box.
The part of the cable that is labeled UF BOX First the display for UF Volume for 3 seconds:
should be connected to the UF calibration box.
UF VOL REF VOL VOL DIFF
Note! The verification will not run (but nothing 1006.5 1007.3 -0.28 %
will get destroyed) if the cable is reversed.

2. Start the AK 95 S with the main switch and Then the display for Isolated UF Volume for 3
the ON/OFF button. seconds:

3. Place B-pickup tube in the acetate-concentrate ISO VOL REF VOL VOL DIFF
container.
1007.5 1007.3 +0.28 %
4. Enter Service mode.
Then the previous display is shown again in
3 seconds and so on.
5. Connect the fluid tubes to the UF calibration
box.
When ready, press to exit the verification of
6. Select Calibration. the UF cell calibration.

7. Select Transducers. Note! Perform a Rinse before leaving the

AK 95 S.
8. Select UF.
The equipment is turned off in the
9. Select Verify. following order:
1 AK 95 S ON/OFF button.
10. Set Reference volume with the SET knob. 2 AK 95 S main switch.
(The Referense volume is labeled on the UF- 3 UF calibration box.
Calibration unit)

11. Press to confirm.

After the Reference volume is confirmed the

verification of the UF cell calibration runs
automatically.

6:20 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005
 Calibrations

BPM-test BPM sub-tests

The functionality of the blood pressure module, In the BPM service menu the following four
BPM, is described in chapter 2, Technical de- subtests are included:
scription.
BPM DIAGNOSTIC MENU
The maintenance to be carried out for the mod-
Inf Def AirLeak PrTest
ule is to check the accuracy for the pressure
transducers, to check the air leakage, to check
- Air leakage test: measures that the equip-
the inflation speed and to check the deflation
ment is airtight.
speed.
- Inflation speed test: measures the time to fill
the cuff with air.
By entering the service menu it is possible to
- Deflation speed: measures the speed of air
perform a check of the BPM device to determine
release from the cuff
if it works correctly.
- Pressure test: Check of pressure transducer
calibration
It is recommended to perform the test at least
every 12 months or after a repair/exchange of
Each of the tests can be carried out separately
the BPM.
but the above order for carrying out the sub-
tests is recommended.
Use following equipment:
- BPM Cuff no.3: 100 900 089
Check that the results from the sub-tests not
- BPM Tubing no. 1 (3,5 m): 100 900 094
exceed following limits:
- BPM test equipment: K2 2151 001 (100 mm
- Air leakage: maximum 18 mmHg/ 3 min
diameter rigid cylinder)
- Inflation speed: 2 -7 s
- Pressure transducer calibration tube kit:
- Deflation speed: 4 - 6 mmHg/s
K13 983 002.
- Static pressure: Maximum deviation from
- Pressure reference instrument: Measurement
reference to be within ± 3 mm Hg.
accuracy of ± 0.8 mm Hg
50 ± 3 mmHg
150 ± 3 mmHg
Test method: 250 ± 3 mmHg
1. Attach cuff no. 3 tight to the BPM test equip
ment, according to the illustrations below:
2. Start the AK 95 S with the main switch and
the ON/OFF button.
3. Enter Service mode.
4. Enter Diagnostics mode.
5. Select BM, BPM
23~33cm
CUFF No. (12cm)
12cm

AK 95 / AK 95 S Service Manual 6:21

HCEN 9138 Rev. 10.2005
Calibrations
Check of air leakage
By entering this test mode the air leakage for the
BPM module, cuff hose and cuff will be tested
automatically. Before entering the test mode cuff
no. 3 and cuff hose no. 1 to be connected to the
AK 95 S. Cuff no. 3 to be wrapped tight to the
BPM test equipment K2 2151 001.
Equipment K2 2151 001 to be used to simulate
the patient arm circumference. The air leakage is
tested for 3 minutes. If the displayed value is
above 18 mm Hg / 3 minutes, this indicates that
there is a possible air leakage in the system.
It is of course also possible to perform this test
without the cuff and cuff hose connected but then
the test will only check for air leakage within the
AK 95 S. In this case replace the cuff and cuff
hose by a tube, which is closed.
Check of inflation speed
By entering this test mode the capacity for the
pump within the module will be tested automati-
cally.
Before entering the test mode cuff no. 3 and cuff
hose no. 1 to be connected to the AK 95 S.
Cuff no. 3 to be wrapped tight to the BPM test
equipment K2 2151 001. If the displayed time is
above 7 seconds, this test indicates that the pump
is worn-out.
Check of deflation speed
By entering this test mode the functionality of
the valves within the module will be tested
automatically. Before entering the test mode cuff
no. 3 and cuff hose no. 1 to be connected to the
AK 95 S. Cuff no. 3 to be wrapped tight to the
BPM test equipment K2 2151 001.
If the displayed value is below 4 mm Hg/second
this test indicates that there is a change in the
restriction for the valves affecting the time for
the air within cuff and cuff hose to be let out.
6:22
Check of pressure transducer
calibration and measured pressure
By entering this test mode it will be possible to
check the actual pressure value for the BPM
pressure on the AK 95 S display.
Before entering the test mode, pressure trans-
ducer calibration tube kit K1 3983 002 to be
connected to the BPM connector on the rear of
the AK 95 S. No cuff and cuff hose to be con-
nected.
Note! The pressure transducer calibration tube
kit must be drained before being connected, to
make sure that no fluids or particles enters the
BPM connector or tubing’s.
When “Measured pressure” is displayed it is
possible to start applying the external reference
pressure.
To check the actual pressure value, external
pressure reference instrument to be connected
and external pressure of 250, 150, 50 mm Hg to
be applied. Check deviation from reference
pressure instrument for displayed value.
Trouble shooting:
If the value from the air leakage test is above 18
mmHg/ 3 minutes, it indicates an air leakage in
the BPM system. First check for any air leakage
on the cuff, cuff hose or the connectors. If the air
leakage does not come from the cuff, cuff hose or
the connectors it indicates that the valves inside
the BPM module are leaking. The BPM module
must then be replaced and sent to Gambro for
repair.
If the result from the inflation speed test ex-
ceeds the limit, first check for any air leakage in
the BPM system. A high value indicates that the
pump capacity in the BPM is too low and there-
fore the BPM module must be replaced and sent
to Gambro for repair.
If the result from the inflation speed test is
below the limit, check that the cuff hose is not
kinked or squeezed and that cuff no. 3 is con-
nected.
AK 95 / AK 95 S Service Manual
HCEN 9138 Rev. 10.2005
 Calibrations

If the result from the deflation speed test is

below the limit, check that the cuff hose is
correctly connected to the cuff and that the tube
is not kinked or squeezed.
Otherwise the failure is caused by the BPM
module and it must be replaced and sent to
Gambro for repair.

If the result from the pressure transducer cali-

bration test is outside the limits, it indicates that
the self-calibration function within the BPM
module not is able to adjust the pressure trans-
ducer output within limits. Possible causes are
pressure transducer or electronic circuits mal-
function. The BPM module must then be re-
placed and sent to Gambro for repair.

AK 95 / AK 95 S Service Manual 6:23

HCEN 9138 Rev. 10.2005
Calibrations

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6:24 AK 95 / AK 95 S Service Manual

HCEN 9138 Rev. 10.2005