Biotech Lab Report- Group 4

1. Parker-Title

2. Parker-Introduction
Koch’s Postulates is a process of assigning causation of diseases by microorganisms
created by Robert Koch. The postulates include 4 requirements that need to be met in
order to qualify causation by microorganisms. These postulates include finding the
microorganism in abundance in all organisms suffering from the disease and not found in
healthy animals, the microorganism must be isolated and grown in a pure culture, the
cultured microorganism must be the cause of the disease in a healthy organism, and the
microorganism must be re-isolated from the inoculated diseased host and identified as
identical to the original agent of causation. For this lab, milk represents the healthy
individual and yogurt represents the diseased. We will be measuring the “yogurtness” of
the milk as the model disease and use Koch’s Postulates to support or refute the
hypothesis.
3. Jenna- Problem
● By using Koch’s Postulates, how can we determine the causative agents of the inoculated
samples?
4. Josh-Hypothesis
If we find that the milk holds more “yogurtness” we can assume that the individual
is diseased or infected.
5. Deven- Experimental Design

Yogurt Milk E.Coli HB101

● Observe the ● Observe the ● Streak E.Coli onto

appearance, smell, appearance, smell, the plate
and pH and pH ● Compare and
● Observe under ● Observe under record the number
microscope microscope of colonies
 ● Streak yogurt onto ● Streak milk onto ● Gently touch E.Coli
the plate the plate plate and inoculate
● Compare and ● Compare and Tube 6
record the number record the number
of colonies of colonies

6. Hailey- Data

Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6

E.coli Yogurt colony Yogurt colony Yogurt + Yogurt Milk Only

#1 #2 amp

7. Elliot- Analysis
Based on the above results, we were successful in isolating the “disease-causing
pathogen.” When introduced to the milk, the bacteria from the yogurt sample caused the
milk to become more yogurt-like. When viewed under a microscope, the sample taken
from the yogurt tube closely resembles the sample taken directly from the yogurt culture.
8. Ahmad- Conclusion
In closing, our hypothesis was supported as the sample that contained more yogurtness
was infected and diseased while the samples that were similar to milk were the healthy
samples. The causative agent can be determined by viewing the samples and meeting 4
requirements which include finding the microorganism in abundance, the microorganism
must be isolated and grown in a pure culture, the microorganisms must be the cause of
the diseases, and the microorganisms have to be reinoculated and identified as the
causative agent.