SPREAD OF TOMATO YELLOW LEAF CURL VIRUS AND TOMATO CHLOROSIS VIRUS TO A NEW

AREA IN PORTUGAL FOLLOWING THE NORTHERN EXPANSION OF THE VECTOR BEMISIA

TABACI
Author(s): D. Louro, H.P. Trenado, I.M. Fortes and J. Navas-Castillo
Source: Journal of Plant Pathology, Vol. 89, No. 2 (July 2007), p. 301
Published by: Società Italiana di Patologia Vegetale (SIPaV)
Stable URL: http://www.jstor.org/stable/41998396
Accessed: 16-01-2016 16:00 UTC

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JournalofPlantPathology(2007),89 (2), 301-305 ^ EdizioniETS Pisa, 2007 301

Disease Note Disease Note

FIRST REPORT OF 'CANDIDATUS SPREAD OF TOMATO YELLOW

PHYTOPLASMA ASTERIS' AFFECTING LEAF CURL VIRUS AND TOMATO
SESAME CULTIVATION IN INDIA CHLOROSIS VIRUS TO A NEW AREA
IN PORTUGAL FOLLOWING
M.S. Khan, S.K. Raj and S.K. Snehi THE NORTHERN EXPANSION
OF THE VECTOR BEMISIA TABACI
Molecular
PlantVirology,
NationalBotanical
Research
Institute,
RanaPratapMarg,Lucknow-2260 01,India D. Louro1,H.P. Trenado2,1.M. Fortes2
and J. Navas-Castillo2
Sesame (,SesamumindicumL.), familyPedaliaceae is
one of the most ancientcultivatedoilseed crops. India 1Estação INIAP,2784-505,
Nacional,
Agronómica
ranksfirstin area (46.5%) undersesamecultivation, pro- Oeiras,Portugal
2Estación "LaMayora ", CSIC,29750,
ducingabout 27.9% of the totalproductionof sesamein Experimental
the world. A phyllodydisease was noticed on sesame Málaga,Spain
Algarrobo-Costa,
plantsgrowingin variousdistricts of UttarPradesh,India
witha diseaseincidenceof 12-20% in 2005. To detectthe The whitefly (Bemisiatabaci)-transmittedTomatoyellow
causal agent,the totalnucleicacid isolatedfrominfected leaf curl virus(TYLCV, genus Begomovirus ) and Tomato
and healthyleaf samplescollectedfromBahraichdistrict chlorosisvirus(ToCV,genusCrinivirus ) havebeendetectable
were blottedon nylonmembraneand hybridizedwitha in theAlgarveregionof southernPortugalsince 1995 and
1998 (Louro et al., 1996;2000). In July2005, an epidemic
probe preparedfroma clone of Chilli littleleaf phyto-
diseaseoutbreakoccurredin open fieldtomatocropsnear
plasma 16S ribosomal RNA gene (GenBank Acc.
DQ343288). All symptomatic plants showed stronghy- CampoMaior(Alentejoregion).Affected plantsshowedleaf
curling,rolling,britdeness,
yellowing, and growthreduction,
bridizationbut none was seen in symptomless samples, associatedwithunusuallyhighpopulationsof B. tabaciin
indicatingan associationof a phytoplasmawiththe phyl- whatwas an EU protectedzone forthisinsect.Of 13 sam-
lody disease. For molecularidentification of this phyto-
ples analysed,all reactedwitha DNA probe specificfor
plasma,PCR was performed usingtotalDNA of infected
TYLCV-typestrain(intergenic region,IR), and sevenreact-
samples and P1/P6 phytoplasma specific universal ed withan RNA probespecificto ToCV (coatproteingene,
primers(Deng and Hiruki,1991) whichresultedin a -1.5 CP). PCR with primers MA-250 (5'GGTGTCCCT-
kb amplicon.Further,nested-PCRwas carriedout with CAAAGCTCTATGGCAATCG3')/MA-118 (5'GATCC-
primersR16F2n/R16R2(Gundersenand Lee, 1996) us- CACATATTGCAAGAC3') (Monci et al., 2002) designed
ing dilutedprimary PCR productas template.The -1.35 foramplification oftheIR ofTYLCV-typestrain, produced
kb ampliconobtainedwas cloned and sequenced. Blast a 310 bp DNA fragmentwith a sequence (GenBank
search analysis of sequence data (GenBank Acc.
DQ333307) 98% identicalto that of a Spanish isolate
DQ431843) showedhighest(99%) identity withthefollow-
(AJ489258)of TYLCV-typestrain.RT-PCR withprimers
ing phytoplasmas: Barleydeformation (AY734453),Aster MA-380 (5'GTGAGACCCCGATGACAGAT3')/MA-381
yellows(AY665676), Onion yellows(AP006628), Silene (5'TACAGTTCCTTGCCCTCGTT3 ') , designedforampli-
virescence(AY744070),Ash witches' -broom(AY566302) ficationof theCP geneof ToCV,produceda 436 bp DNA
and Maize bushystunt(AY265208),membersof 'Candida- fragment with a sequence (DQ335133) 100% and 99%
tes phytoplasma asteris'(16SrI) group.To our knowledge, identicalto the CP genes of ToCV isolatesfromAlgarve
thisis thefirstreportof molecularidentification of 'Candi- (DQ335134) and Spain(DQ136146) respectively. Thisis the
dates phytoplasma asteris'(16SrI group) affecting sesame firstreportofthetypestrainofTYLCV in Portugal,and of
in India. Recendya witches'
cultivation -broomdisease of TYLCV andToCV inAlentejo.
sesamein Oman has been assignedby RFLP analysisto a Louro D., NorisE., VerattiF., AccottoG.P., 1996.Firstre-
different
group(16srII)(Al-Sakeiti etal.,2005). PlantDis-
portoftomatoyellowleafcurlvirusinPortugal.
M.A.,Al-Subhi
Al-Sakeiti A.M.,Al-Saady N.A.,DedmanM.L., ease80: 1079.
2005.Firstreport ofwitches'-broomdiseaseofsesame(.Sesa- Louro D., AccottoG.P.,VairaA.M.,2000. Occurrenceand
mumindicum ) inOman.PlantDisease89:530. diagnosisof Tomatochlorosis virusin Portugal.European
S., Hiruki D., 1991.Amplificationof 16S rRNAgenes Journal ofPlantPathology 106:589-592.
Deng
fromculturable and nonculturablemollicutes.Journalof MonciF., Sánchez-Campos S., Navas-CastilloJ.,MoñonesE.,
Microbiological Methods14:53-61. 2002.A natural recombinantbetween thegeminivirusesToma-
GundersenD.E., Lee I.M., 1996.Ultrasensitive detectionof toyellowleafcurlSardiniavirusand Tomato yellow curl
leaf
a novelpathogenic
virusexhibits phenotype andis becoming
phytoplasmas by nested-PCRassaysusingtwo universal
Mediterranea 35: 144-51. inSpanish
prevalent populations.Virology303:317-326.
primer pairs.Phytopathologia
S.K.Raj
author.
Corresponding Corresponding J.Navas-Castillo
author.
Fax:+091.522.2205836 Fax:+34.95.2552677
E-mail:
skraj2@rediffmail.com E-mail:
jnavas@eelm.csic.es
Received 4,2007
January Received 11,2007
January
Accepted 30,2007
January Accepted 4,2007
February

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