Biosafety Level II

Biosafety Level 2
Introduction

uitable for work involving agents

of moderate potential hazard to
personnel and the environment.

2.4
 Biosafety Level 2
Introduction

Examples:
n Measles virus
n Salmonellae
n Toxoplasma spp.
n Hepatitis B virus

* Immunization or antibiotic treatment is available

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 Biosafety Level 2
Introduction

Examples:
n Bloodborne pathogens
n Human body fluids/particularly when visibly
contaminated with blood

* Extreme precaution with contaminated needles or

sharp instruments

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Examples of Blood borne Pathogens

„ Human Immunodeficiency Virus (HIV)

„ Hepatitis B Virus (HBV)
„ Hepatitis C Virus (HCV)
Biosafety Level 2
Facility Design (Secondary Barriers)

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 Biosafety Level 2
Facility Design (Secondary Barriers)

Requirements:
§ Laboratories have lockable doors
§ Sink for hand washing
§ Work surfaces easily cleaned
§ Bench tops are impervious to water
§ Sturdy furniture

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 Biosafety Level 2
Facility Design (Secondary Barriers)

Requirements (cont.):
§ Biological safety cabinets installed as
needed
§ Adequate illumination
§ Eyewash readily available
§ Air flows into lab without re-circulation to
non-lab areas
§ Windows fitted with flyscreens
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Biosafety Level 2
Facility Design (Secondary Barrier)

Restricted
access when
work in
progress

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 Biosafety Level 2
Laboratory Facilities (Secondary Barriers)

n BSL-1 Facilities PLUS:

n Autoclave
available
n Eyewash station
available

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 Biosafety Level 2
Facility Construction (Secondary Barrier)

Requirements:
n Location - separated from public areas
n Structure - normal construction
n Ventilation - directional

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Biosafety Level 2
Standard Microbiological Practices

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 Biosafety Level 2
Safety Equipment (Primary Barriers)

n Use biosafety cabinets (class II) for

work with infectious agents involving:
n Aerosols and splashes

n Large volumes

n High concentrations

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 Biosafety Level 2
Safety Equipment (Primary Barriers)

n Class II Biosafety
Cabinet
n Airflow

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 Biosafety Level 2
Safety Equipment (Primary Barriers)

n Class II Biosafety Cabinet

n Equipment layout

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 Biosafety Level 2
Safety Equipment (Primary Barriers)

n Class II Biosafety
Cabinet
n Technique

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 Biosafety Level 2
Special Practices

Supervision
§ Supervisor is a competent scientist with
increased responsibilities
§ Limits access if immunocompromised
§ Restricts access to immunized

Lab Personnel
§ Aware of potential hazards
§ Proficient in practices/techniques
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 Biosafety Level 2
Special Practices

Needles & Sharps Precautions

n Use sharps containers
n DON’T break, bend, re-sheath or reuse
syringes or needles

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 Biosafety Level 2
Special Practices

Needles & Sharps Precautions (cont.)

n DON’T place needles or sharps in office
waste containers

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 Biosafety Level 2
Special Practices

Needles and Sharps Precautions (cont.)

n DON’T touch broken glass with hands

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 Biosafety Level 2
Special Practices

Needles and Sharps Precautions (cont.)

n Use plasticware

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 Biosafety Level 2
Special Practices

n Policies and procedures for entry

n Biohazard warning signs
n Biosafety manual specific to lab
n Training with annual updates

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 Biosafety Level 2
Special Practices

n Use leak-proof transport containers

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 Biosafety Level 2
Special Practices

n Decontaminate work surfaces

n Report spills and accidents
n No animals in laboratories

2.4