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Alana Dass: SS-Galactosidase Project
Alana Dass: SS-Galactosidase Project
Experience Laboratory Assistant, Cornell Molecular Biochemistry Department Aug 2019 – May
2020
ß-Galactosidase Project
Performed Bradford Assays on DEAE Cellulose chromatographic fractions
Executed lab electrophoresis techniques to ascertain ß-gal molecular identity
Green Fluorescent Protein Purification Project
Performed nickel-affinity purification of GFP-binding nanobody from E. coli
Utilized gel-filtration chromatography to isolate GFP-binding nanobody and immunoprecipitated
Vtc1-GFP from S. cerevisiae
Ran SDS-PAGE and Western Blotting to ensure isolation of Vtc1-GFP
Stress Response/Unfolded Protein Response Pathway Project
Extracted, purified, spliced RNA from S. cerevisiae, synthesized cDNA
Employed CRISPR-Cas9 editing to create temperature-sensitive genes
⊱ Engineered plasmid via RF cloning; used Sanger sequencing for verification
Performed quantitative RT-PCR to determine gene expression
Skills Gel Electrophoresis, Molecular Staining, Mass Spectrometry, IR spectrometry, Sterility Practices,
Literature Review, Qualitative Research, Quantitative Research, Data Entry and Analysis, Root Cause
Analysis, Microsoft Office, MATLAB, Swiss PDB Viewer, BIO-RAD Image Lab, PYMOL, Protein
Databank, Project Management, Communication Skills