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Activity #3
Activity #3
EXPERIMENT NO. 3
II - DISCUSSION:
Apparatus:
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Exercise in Pharmaceutical Analysis II
B. UV / VIS Spectrophotometer
Spectrophotometer- is a photometer (a device for measuring light intensity)
that can measure intensity as a function of the color and wavelength of the
light.
Parts:
Readout device
⮚ Use the current or voltage produced by the detector and amplifies it.
⮚ The readouts device then converts the Current or Voltage into a form that
analyst can read as a print out.
Principle involved:
⮚ The sample absorbs a portion of the incident radiation; the remainder is
transmitted to a detector, where it is changed into an electrical signal and
displayed usually after amplification, on a meter, chart recorder or some
other type of readout device.
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Exercise in Pharmaceutical Analysis II
IV - ASSAY PROCEDURES:
Place 900 ml plain, distilled water in each of the six – 1L glass beakers. Place the
beakers on the hot plate and heat until it reaches 37⁰C (+/-5). Monitor the
temperature with a thermometer. Keep the temperature constant. Set aside.
Weigh no less than 10 capsules and take the average weight. Take six
capsules and weigh individually in the analytical balance for use in the test.
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Exercise in Pharmaceutical Analysis II
C. Operating Procedures
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Exercise in Pharmaceutical Analysis II
2. Make sure that the machine is placed on top of flat and stable top. Use level
instrument to make sure the level of the machine.
3. Check if the voltage match and make sure they are connected correctly.
Connect the 2 types of the connectors for upper and lower seats. Make sure
that they are connected correctly and lock tight.
4. Feed water into the water tank to appropriate level. The round bottom tank is
placed inside the water tank to prevent floating switch stuck.
5. Push the beaker at the right rear side to on the start power.
6. Press POWER to start power.
7. Press JACK to move axis base up or down to the fixed position
8. Press HEAT to start heating system and circular system, set up the
temperature to maintain constant temperature.
9. Press STIR to start mixer system. Adjust the speed adjustment knob to the
required speed.
10. When assembling and dismantling the paddle blades and test basket move
the axis base to upper position. Tighten the screw.
11. Cover the beaker and maintain temperature inside the cup.
12. After use, store the accessories properly. Keep the machine clean.
Time 90 minutes
Wavelength 272 nm
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Exercise in Pharmaceutical Analysis II
USP Specifications Not less than 80% of the labeled claim of amoxicillin
is absorbed in 90 minutes.
CALCULATIONS:
The concentration of the Amoxicillin per capsule and its compliance with the USP
requirement may be calculated as follows:
Note: if the capsules used contain 500 mg of Amoxicillin, the aliquot portion
equivalent to 0.5 mg Amoxicillin is 0.9ml, which is calculated as follows:
900ml (0.5mg)
X= = 0.9ml
500mg
900 50
DF = X = 50,000
0.9 1
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Exercise in Pharmaceutical Analysis II
WORK SHEET
EXPERIMENT NO. 3
RESULTS OF ANALYSIS:
2.730
10 ug/mL
50,000
Dilution factor
3.46 g
0.4325 g
Amount % Labeled
obtained claim
Capsule # Actual wt. Au readings
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Exercise in Pharmaceutical Analysis II
2. Calculations
Certified Correct:
_________________________
Signature over Printed Name
Of QC Inspector
Date: __________________
1. In the dissolution test for capsules, why is necessary to use the cylindrical
basket instead of the paddle?
It is because the pores in the mesh allow the dissolving drug to move from the
basket into the holding vessel, creating a uniform solution. The sticky capsules
containing HPMC or similar compounds as a binder are tested using a basket because
tablet swells in water & may stick to the walls of the dissolution vessel.
2. Why is the sampling procedure done at a point midway between the tip of
the basket and the surface of the dissolution medium?
Due to the reason that it is where the concentration is exact. The sample at the
bottom of the vessel is at is concentrated state while at the top is diluted.
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Exercise in Pharmaceutical Analysis II
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