Developing B cells

Flow Cytometry to determine # of

BM and 20 tissue
plasma in
Lymph
CD 19 Positive cells
Immature B

't
n :÷ ÷ ÷ ÷ ÷ ÷ i÷ :
e
or '
n
*
A Amount of
.
CD 19

B
GD
.
I

3 surface proteins
1
"
CD 19 detection All developing mature B

Afm!% 'd!
3 diff flow

tugs each
:'( 24 specific

3
'd
specific
to

to
Engin
A region
od

of
Eg D
IgM
mature Balls B cells will
A. CD 19 is found in
developing .

from a hemi poetic stem cell

begin displaying
CD 19 after differentiating and aceBo Ball
a .

Bulls will stop expression of 61719 when I pointed to

they become plasma cells .

where B tells ace module and will express both IgM and
IGD .
 B mature B plasma
anecgic

÷ ÷ :÷ ÷i ÷ ÷ ÷ :
'

.
¥.
i
.

IgM
"

IgM
-
-

Ign

÷ ÷: ÷ ÷ ÷ ÷ ÷ ÷ ÷ \
-
÷ ÷ ÷ ÷ ÷ ÷i :

I
GD Ig
D
Ig D
B .

Immature B cells express IgM and some D indicated

Ig by
a
majority of
signals in the t
Ig M zone of the plot
An Bulls high IGD and how Cirone )
IgM as i dilated
generic express
"

and
by signaling in the
Igbt Ig M
-

zone of the plot

Mutua Bulls express

high Ig D and low
IgM .
This is shown

by signaling in the t
D
Ig and t
IgM .

Plasma B
-
I
-

zone .
alls some have no
IgDoc IgM expressed indicated
by signal in