Professional Documents
Culture Documents
Hasil Penelitian Translate
Hasil Penelitian Translate
Abstract. Kipahit leaf which contains tannic acid is highest among the five plants that have been identified
previously. Therefore, the researcher conducted further tests on the effect of antidiabetic mellitus type 2 kipahit leaf
(Tithonia diversifolia) to the decrease of blood sugar levels of the white male mice. The sample consisted of 25
white male mice. They were taken randomly and then divided into five groups; each group consisted of 5 mice. The
first group was normal group given aquadest only, the second group is a comparison group with the giving of
glibenclamide (0.26 mg / 20 weight of mice), the third group is a tested group with an infusion of leaves kipahit by
the dosage I (0,13mg /20 g weight of mice), the fourth group is a tested group with an infusion of leaves kipahit by
dosage II (0,26 mg /20 g weight of mice), the fifth group is a tested group with an infusion of kipahit leaf by the
dosage III (0,52mg / 20 g weight of mice). The method used in this research is glucose tolerance test. The result of
data graph and statistical analysis of variance (ANOVA) giving infusion of kipahit leaf effect on blood sugar levels
decrease significantly with the level of α = 0.05. The optimal result is shown in fifth groups (dose III) that given by
preparation with a concentration of 0.52 mg / weight of mice significantly with the level of α = 0.05
normal and slowly but definitely will break body tissue if not handled precisely
(Nurkhozin et al., 2011). This disease associated with the role of oxidative stress.
Oxidative stress is an imbalance results from the increase of radical system and radical
scavenging systems that has increased the production of free radical or reduced
by combination of genetic and environmental factors. Many patients with diabetes type
2 have family members who also suffer diabetes type 2 or other health problems
associated with diabetes, such as high blood cholesterol, high blood pressure
(hypertension) or obesity. Environmental factors that influence the risk of diabetes type
usually recommend to consume drugs namely Biguanide and Glitazon types. These two
drugs have the same effect toward the body that can increase the sensitivity of body
Beside chemical drugs, many herbs used as medicine. Antioxidant compounds found in
plants have potential as an antidiabetic that able to prevent the oxidation of glucose in
the blood (Rosiyana, 2012). It is known that compounds tannic acid (tannin) has
the content of tannic acid. It shows the potential of these plants as antidiabetic drugs.
Seeing this potential, researchers feel need to conduct further tests in the form of Effect
Animal Model. Swiss Webster white male white mice weighing between 20-25 grams
obtained from the Animal Laboratory of School of Life and Environmental Sciences of
RESEARCH METHOD
Making Kipahit Leaf Infusion (Thithonia diversifolia). Kipahit leaves are boiled
with 100 ml of aquadest in infusion pan for 15 minutes at 90-950C, then filtered and
diversifolia)
Animal models are divided into 6 groups, each group consists of 6 mice, namely:
3. Group dose I was given infusion of kipahit leaf dose 5.6 mg / 20 gram of mice
weight
4. Group dose II was given infusion of kipahit leaf dose 11,2 mg / 20 gram of mice
weight.
5. Group dose III given infusion of kipahit leaf dose 22,4 mg / 20 gram mice weight.
Research procedure:
1. Mice are fasted for 6 hours before testing begins and still given drink.
2. Measure blood glucose levels for each mouse, measured as baseline blood glucose.
4. After 30 minutes, measured again blood glucose levels of the mice, then the mice
5. Measure blood glucose levels in the minutes of 15, 30, 45, 60 and 90.
RESULT AND DISCUSSION
Test Method of Effect of Anti Diabetes Melitus Type II towards Swiss Webster
Mice
The results of antidiabetic activity test towards Swiss Webster white mice was
conducted using several dose variations, ie dose 5,6 mg / 20 gram of mice weight; 11,2
mg / 20 gram of mice weight and 22,4 mg / 20 gram of mice weight. Giving glucose
0.013 mg / 20 gram of mice weight. The results showed that kipahit leaf infusion was
Table 1.
Table of blood sugar levels average of mice before and after treatment
Group III (Dose 1) 89,2 199,2 127,4 117,4 108 91,2 mg/dl
Remarks:
- T0 = minute(s) of 0, early blood glucose of mice was checked, then given glucose
solution.
- T1 = minute(s) of 30, blood sugar level of mice was checked and then given the
- T2 = minutes(s) of 45, blood sugar level of mice was checked after 15 minutes of
treatment
- T3 = minute(s) of 60, blood sugar level of mice was check after 30 minutes of
treatment
treatment
From the table above, it can be seen the results is mostly at minute 0 mice
were in the normal blood sugar levels, then rose to above normal blood sugar levels at
kipahit leaf gives effect to decrease blood sugar level of mice, researcher analyze by
using analysis of variance (ANAVA) in one direction because this research can be done
at one time. The research used sample homogeneous test. Source of data used the
Table 4.5.
Decrease of t Mice
Total Average
Blood Sugar Levels 1 2 3 4 5
Group 1 (aquadest) 1 2 0 4 16 23 4,6
Group 2 (glibenclamide) 125 130 126 128 110 619 123,8
Group 3 (dose 1) 86 83 89 94 104 456 91,2
Group 4 (dose 2) 102 99 101 106 108 516 103,2
Group 5 (dose 3) 95 116 107 103 110 531 106,2
Remarks:
weight.
- Group 3: dose I was given kipahit leaf infusion (Tithonia diversifolia) dose 5.6 mL /
- Group 4: dose II was given kipahit leaf infusion (Tithonia diversifolia) dose 11.2 mL /
- Group 5: dose IIII was given kipahit leaf infusion (Tithonia diversifolia) dose 22,4
Tabel 4.6.
Blood Sugar
Levels Decrease F F
Remarks:
- H0: giving extract does not affect to decrease mice blood sugar level.
- H1: giving infusion affects the decrease blood sugar levels of mice.
- F count > F Table, means: H0 rejected, H1 accepted.
Conclusion: giving infusion effects on decreasing mice blood sugar level. It is because
duncant test DMRT (Duncant Multiple Range Test). For Sy = 3.15658. So in the results
Table 4.7.
P 2 3 4 5
rp
with r value for P 2, 3, 4 and 5 from the duncant test table. This value functions to
determine the boundaries (lower limit and upper limit) of the test group.
Table 4.8.
Duncant Test 1 2 3 4
Group I (aquadest) 4,6
Group III (dose 1) 91,2
Group IV (dose 2) 103,2
Group V (dose 3) 106,2
Group II
(glibenclamide) 123,8
Based on the above table, it can be concluded that treatment of dose 1, 2, 3 were
level, the best result was obtained by difference of 106,2 mg/dl, occurred in mice given
infusion dose 3. Although dose 2 and dose 3 were different but the difference between
the effects of both doses was not significant. Even though dose 3 still had the best
effect on the decrease in blood sugar levels of mice as they were closer to
Table 4.9
Decrease (mg/dl)
dose 1 91,2
dose 2 103,2
dose 3 106,2
blood sugar levels decrease of mice from the minutes of 45 to the minutes of 90, it can
decrease of mice
Based on the diagram above, it can be seen every addition of dosage infusion, giving an
increased effect on the decrease blood sugar levels of mice.
CONCLUSION
Giving infusion of kipahit leaf (Tithonia diversipolia.) able to decrease blood glucose
level.
a. Kipahit leaf infusion (Tithonia diversipolia) dose 3 (22.4 g / 20 g of mice weight) has
REFERENCES
Dalimartha, S. 1999. Ramuan Tradisional Untuk Pengobatan Diabetes Mellitus.
Hopkins, J., 2012. Methods for Delivering Insulin and Monitoring Blood Sugar A
Review of the Research for Children, Teens, and Adults With Diabetes.
Hutapea, J.R. 1994. Inventaris Tanaman Obat Indonesia. Edisi III. Jakarta:
Phytochemistry. Vol.2(1):171-175.
Mutschler, E. 1999. Dinamika Obat. (Penerjemah: Mathilda B.W, Anna S). penerbit
Bandung
Mada. Yogyakarta.
World Health Organization. 2002. WHO traditional medicine strategy 2002-2005. Worl