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Bioscreen User Manual 2015
Bioscreen User Manual 2015
Bioscreen User Manual 2015
Bioscreen C
Oy Growth Curves Ab Ltd
2015
Version 2.1.11
Table of Contents
2. System Description 3
2.1 Overview 4
2.2 Bioscreen C Reader 4
2.3 Software 5
3. Operation 6
3.1 Pre-Start Checklist 6
3.2 Planning an Experiment 6
3.3 Running an Experiment 7
3.4 Data Handling 10
6. Maintenance 12
6.1 Maintenance Steps & Frequency 12
6.2 Cleaning 12
6.3 Checking the Heat Transfer Fluid Level 13
6.4 Replacing the Fuses 13
6.5 Replacing the Lamp 14
7. Troubleshooting Guide 16
7.1 Optical Problems 16
7.2 Temperature Problems 17
7.3 Operational Problems 18
8. About Bioscreen 19
8.1 Product History 19
8.2 Unique Features of Bioscreen C 19
8.3 Applications 20
9. Technical Specifications 22
9.1 General Specifications 22
9.2 Safety Specifications 22
9.3 Photometric System 23
9.4 Incubator 23
9.5 Honeycomb 2 Microplates 23
9.6 PC Requirements 24
9.7 EU Directive on Waste (WEEE) 24
11. Appendix
Warranty Statement
12. Appendix
Bioscreen C Service Request
Bioscreen C Certificate of decontamination
Bioscreen C Microbiology Reader User's Manual
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Lamp and
Incubator compartment:
filter wheel
Measurement 2 Honeycomb Microplates,
compartment
compartment sample tray
Main cover
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Bioscreen C Microbiology Reader User's Manual
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It is very important to read these instructions carefully and to follow them in the presented order
(from 1 to 11). Wrong order might cause problems with the installation or the use of the Bioscreen C
system.
Check that the incubator cover is placed properly on its hinges and that the cover is locked in its place. White
arrows on the incubator cover point to the hinges (see the notification leaflet for more information).
Before turning the Bioscreen C on ALWAYS remember to check that the incubator cover is in its
right position. Improper placing of the cover can cause serious damage to the instrument which
is NOT covered by warranty!
Always close the lids before starting an experiment, as placing fingers etc. inside a running
instrument can cause serous physical injury.
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Bioscreen C Microbiology Reader User's Manual
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Figure 2 Positioning of the filter wheel, the halogen lamp and the incubator cover.
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2. SYSTEM DESCRIPTION
2.1 Overview
The Bioscreen C Microbiology Reader is a fully automated system developed to perform a wide range of
microbiology experiments. The system consists of:
• Bioscreen C reader
• BioScreener software
• Honeycomb microplates (consumables)
• PC (not provided by Growth Curves Ltd.)
All microorganisms (bacteria, mold, yeast, etc.) increase the turbidity of liquid growth medium when growing
and multiplying in it. Bioscreen C monitors this growth by measuring the turbidity of the medium in the wells of
a microplate. These measurements are done kinetically, and recorded as optical density (OD)
measurements. All functions are controlled by BioScreener software according to the parameters entered by
the user. OD values are recorded automatically by the software.
Bioscreen C is an open system: the user can decide what is studied in the the wells. Any microorganism,
broth or chemical can be pipetted into the wells for monitoring growth kinetically and automatically. Most of
the conventional test tube tests where the turbidity or color change occurs can be run with the "mini-tubes" in
a Honeycomb microplate with Bioscreen C.
For vertical light photometry, all visible light wavelengths can be used. The eight standard filters provided in
the filter wheel of Bioscreen C are: 405, 420, 450, 492, 540, 580, 600 nm and a wide band (420-580 nm)
filter. Filters with special wavelengths are available by request.
2.2.2 Incubator
The incubator consists of the incubator tray (fixed, non-removable), the incubator cover (on hinges) and a
temperature control system. After filling the microplate with experimental materials, the incubator cover is
placed manually on top of the incubator tray and properly closed. During the incubation and measuring, the
incubator cover remains in place.
The temperature control system maintains the incubation temperature set by the program. Ethylene glycol
and water mixture (1:1) is used as a heat transfer liquid. The liquid circulates continuously in the incubator
tray to keep the temperature stable and at a selected value. The temperature is continuously monitored and
saved by the software.
A halogen lamp produces light which then passes through the chopper wheel. The light path is turned 90
degrees by a mirror. Then the light passes through the filter wheel. The interference filters are selected with
the PC software. Filtered light moves through an optical fiber to the lens assembly in the measurement
compartment, below the Honeycomb microplates. The light passes through the bottom of each well and
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Bioscreen C Microbiology Reader User's Manual
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results are collected by the detector. The detector is on a retractable arm which moves into the right places
above the plate.
The measuring device is moved along the Y-axis while the incubator tray with the microplate is moved along
the X-axis. This enables rapid measurement of all wells in the microplate.
Detector
UV-mirror
Chopper wheel
2 Honeycomb
microplates with
covers (200 samples)
Lens
Optical fiber
Filter wheel
Halogen lamp
2.3 Software
®
The BioScreener software is designed to run Bioscreen C experiments. BioScreener SW is a combination of
the proven functions of the previous softwares used with Bioscreen C.
BioScreener stores all settings and results from the experiment in a .bsm file. Results can be exported to
common data files such as xls or csv for graph generation and analysis with another software of your choice.
Please remember to save the data on a local hard drive, do not use network disks. You can always
transfer the measurement files to a network disk after the experiment is completed. This way your experiment
won’t be interrupted by possible network errors.
The instrument can handle one or two 100-well Honeycomb microplates at a time. Each well of the microplate
can be considered as an individual test vessel. Thus monitoring the microbiological growth in 200
experiments can be performed simultaneously in a single run.
Honeycomb microplates are sterilized by gamma radiation, packed in individual bags and sold in boxes of
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Bioscreen C Microbiology Reader User's Manual
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Honeycomb multiwell microplate technology positions 100 wells in a similar footprint as a 96-well microplate.
The microplate was designed to assure the best temperature distribution across the microplate, as well as to
eliminate evaporation and condensation, common problems with conventional 96-well microplates.
The unique incubator of Bioscreen C has liquid circulation which is cabable of cooling the samples below the
ambient temperature and ensures constant temperature to all wells. This is essential for developing high
quality growth curves.
3. OPERATION
When choosing the sample volume, consider also possible behaviour of the sample during its incubation and
the entire experiment. If the microorganisms in the sample are supposed to create gas, make sure that you
don’t fill the wells too much. Flooding of the wells can cause damage to the parts underneath the incubator
tray.
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Bioscreen C Microbiology Reader User's Manual
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60 °C
50 °C
Temperature
40 °C
30 °C
20 °C
10 °C
0 °C
25 °C
0 °C 10 °C 20 °C 30 °C 40 °C 50 °C
Ambient temperature
Example 1. At ambient temperature 10 °C the incubating temperature can be set at 4 - 40 °C.
Example 2. At ambient temperature 20 °C the incubating temperature can be set at 14 - 50 °C.
Example 3. At ambient temperature 30 °C the incubating temperature can be set at 24 - 60 °C.
Figure 4 Range of incubating temperatures that can be selected at different ambient temperatures.
3.2.4 Filters
Up to 3 of the eight filters on the filter wheel can be chosen to be used in a single measurement. The
wavelengths of the standard filters are 405-600 nm. The wideband filter (420-580 nm) is less sensitive to
color changes than the other filters so the results are not affected so much by change of color of growth
medium.
3.2.5 Shaking
BioScreener software has various shaking protocols. It also includes parameters from previous Bioscreen C
softwares (eg. EZExperiment SW and BioLink SW), which can be used by selecting appropriate compatibility
mode from BioScreener’s Additional options menu.
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Bioscreen C Microbiology Reader User's Manual
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of the tray. Put the incubator cover back carefully, making sure that the hinges fit through the slots in the back
of the cover. Close the lock on the front side: snap the front of the cover into the catch. See Figures 5 & 6.
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Bioscreen C Microbiology Reader User's Manual
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If necessary, the cover of the incubator compartment can be opened between the measurement cycles.
Press Pause in the BioScreener software’s Settings window. Make sure that the Bioscreen has come to a
full stop and it is safe to open the cover. New samples can be added or samples for other studies can be
taken. Continue the measurement by pressing the Continue button in the software.
When the incubator cover is opened, condensation may occur onto the microplate lid. If this occurs,
adjust the time between the measurements so that the heating of the incubator cover has time to
evaporate the condensation before the next measurement.
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This approach lets the user handle Bioscreen C data (curve drawing etc.) using any DOS, OS/2 or Windows
graphical, scientific or statistical software.
All use of BioScreener software is done entirely at the users own risk. Growth Curves Ltd.
assumes no liability for any problems the user of the software might encounter, including
but not limited to any loss of data, samples or any of the similar.
Growth Curves Ltd. assumes no liability for software developed by other companies.
Any and all claims relating to software shall be addressed to the licensor or developer.
Strict anaerobes:
1. Samples are pipetted into wells of Honeycomb microplate in an anaerobic environment. The two
"ears" of the Honeycomb microplate are cut off and the microplate is hermetically sealed with
appropriate glue or tape.
An indicator liquid or paper is placed into one well for confirming the existence of anaerobiosis. If O2
leaks, the colour of the indicator liquid or paper changes. The hermetically closed Honeycomb
microplate is put into the Bioscreen C and a normal experiment with shaking, incubating and
measurement is carried out.
1. Oil layer technique can be used. The oil diminishes the dissolving of O2 from air into the sample.
2. Redox medium (including chemicals which react with O2) can be used with previously described
techniques or separately.
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Bioscreen C Microbiology Reader User's Manual
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Keep this paper under the Honeycomb microplate during sample pipetting.
The well numbers are seen through the wells of the microplate.
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6. MAINTENANCE
6.2 Cleaning
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The heat transfer fluid bottle is located inside the Accessory pack included with your system. To add more
fluid, do the following:
The heat transfer fluid contains ethylene glycol, which is harmful if swallowed. Keep the bottle clearly
labeled and wash your hands after the use of the fluid.
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Bioscreen C Microbiology Reader User's Manual
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Replace the blown fuses only with fuses with the same rating shown on the serial plate of the
instrument. Failure to do this may cause instrument damage and/or personal injury.
CAUTION:
THE LAMP MAY BE VERY HOT! Do not touch it with bare hands or let it cool down before changing.
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There are some brands of lamps that will not give enough light for the wavelength range that the
Bioscreen C operates. Only lamps that are sold and pre-tested by Growth Curves Ltd. are
guaranteed to operate properly.
Use only these certified lamps in order to avoid the F1-b2 / “too little light” error!
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7. TROUBLESHOOTING GUIDE
Solution 1) Change the lamp to a pre-tested and approved lamp marked with red dot.
2) Check the detector lens for scratches.
3) Clean the filters and lenses.
Note Sometimes old lamps can cause problems. They are lit, but the amount of ultraviolet
light is not sufficient for the measurement. This can cause false assumption that the
lamp is OK when it is not.
Cause 1) Sediment accumulates to the bottom of the sample wells and it moves randomly
when the carriage moves.
2) The cover has been opened during the measurement and this has elevated the
measurement values.
3) Ambient temperature is too high or too low to keep the programmed incubation
temperature.
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Bioscreen C Microbiology Reader User's Manual
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Solution 1) Use shaking before each measurement to keep the sample suspension
homogenous. Try different shaking options for the best result.
2) Do not open the cover during the measurement.
Problem Elevated readings which lower gradually to normal after a few minutes.
Cause The vapors from the sample have condensed onto the microplate lid. This happens
when measurement temperature is lowered during the measurement, or when the run
has been aborted and started again, or when the incubator cover has been removed
during the measurement.
Solution Make the measurement start from lower temperatures and measure the warmest last.
If reverse temperature measurements are needed, give the instrument enough time to
stabilize at the desired temperature before measurement. If condensation occurs
while the experiment has been aborted, warm up the instrument and samples as
usually is done at the start of the experiment.
Cause The incubator tray is loose in its carriage. The locking screws are loose and the
incubator is not firmly secured in its place.
Solution If the carriage locking screws are loose, tighten them firmly (but not too tight).
Note With very old instruments this behavior can be caused by external electromagnetic
interferences. Newer instruments have protective coating on the chassis to prevent
against electromagnetic (EMP) disturbances.
If the problem arises after the instrument has been relocated (or other changes have
been made in the operational environment), this is the probable cause. Try to solve
the problem by using the instrument in another location, or use better regulated power
outlets (reserved for computer use only).
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Cause Computer power saving features interfere with serial port communication.
Solution Turn off all power saving features from your computer.
Please consult with your IT department if necessary.
Problem Motor runs the incubator against an obstacle which causes noise.
Cause The incubator tray cover is not placed properly over the hinges.
The cover crashes against the optical detector assembly.
Solution Place the cover properly over the hinges. Wiggle it to be sure it’s in place (See figures
5 & 6).
Check for damage on the upper lens (in the detector assembly). If the lens has been
damaged, it must be replaced.
Cause The incubator tray cover is not placed properly over the hinges. The cover crashes
with the detector assembly.
Solution Place the cover properly over the hinges. Wiggle it to be sure it’s in place (See figures
5 & 6).
Check for damages on the upper lens (in the detector assembly). If the lens has been
damaged, it must be replaced.
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Bioscreen C Microbiology Reader User's Manual
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8. ABOUT BIOSCREEN C
After Labsystems Oy was acquired by Thermo Electron, the strategic decision was made to sell off the
Bioscreen C line to a newly-formed company, Oy Growth Curves Ab Ltd. (Helsinki, Finland). Oy Growth
Curves Ab Ltd. now manufactures the Bioscreen C instrument for distribution throughout the world.
8.3 Applications
Bioscreen C can measure growth of any organism that will increase the turbidity of the growth medium when
growing and multiplying in it. In addition to monitoring the growth of microorganisms (bacteria, yeast, mold,
etc.), the growth of fungi, algae, phage and suspended cell cultures can be monitored by Bioscreen C.
Some examples of applications for the system include: microbiology research, development of new
antibiotics, antibiotic susceptibility tests, protein expression, development of new preservatives, optimization
of fermentation processes, development of waste treatment processes, bioassays, kinetic MIC, QC of foods,
cell culturing, growth medium development, tests to study effects of pH, temperature, chemicals or other
parameters on microorganisms etc. and many others.
Even anaerobic bacteria can be grown in Bioscreen C. This can be done by sealing the cover of the
microplate and using an oxygen-free gas, alternatively the oil layer technique can be used (see chapter “4.
Guide to anaerobic experiments” ). One can also monitor bacteriophage growth (dropping growth curves),
amoebae (Limulus) growth and other processes where the turbidity changes in any way.
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Bioscreen C Microbiology Reader User's Manual
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To get a closer view of the research area you are interested in, please make a search at Google Scholar. Use
a search phrase: “Bioscreen C” and then add the specific area you are interested in, for example “algae”.
Most likely you will get a convincing list of public studies done in the field of your interest.
A user can dispense any micro-organism with any broth and chemical to the microplates to monitor kinetically
the growth of the culture i.e. the development of turbidity or color in 200 wells at a time. Most tests that are
run in test tubes with broth can be run automatically by Bioscreen C.
Examples of Applications:
• Microbiological assays for measuring the amount of vitamins, amino acids, antibiotics, disinfectants,
toxicants, biostimulators, growth retardants, or other chemicals.
• To measure the combined effect of various parameters (e.g. pH, temperature, water activity, salt
concentration, chemicals) on pure and mixed cultures.
• LAL tests (Limulus amoebocyte lysate gel assay) for endotoxin assay.
• Mathematic modeling of microorganism, phage or cell growth with the help of MS Excel or similar
softwares.
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9. TECHNICAL SPECIFICATIONS
Weight: 22 kg
Dimensions (WxDxH): 62 cm x 38 cm x 23 cm
The safety specifications are met also under the following environmental conditions in addition or in excess to
those stated in the operating conditions:
Altitude: up to 2000 m
Temperature: +5 °C – +40 °C
NOTE 1 Installation category (over-voltage category) defines the level of transient over-voltage, which the
instrument is designed to withstand safely. It depends on the nature of the electricity supply and
its over-voltage protection means. For example in CAT II which is the category used for
instruments in installations supplied from supply comparable to public mains such as hospital
and research laboratories and most industrial laboratories the expected transient over-voltage is
2500 V for a 230 V supply and 1500 V for a 120 V supply.
NOTE 2 Pollution degree describes the amount of conductive pollution present in the operating
environment. Pollution degree 2 assumes that normally only non-conductive pollution such as
dust occurs with the exception of occasional conductivity caused by condensation.
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Both of these affect the dimensioning of the electrical insulation within the instrument.
Resolution: 0.001A
Filters: 8 filters: 405, 420, 450, 492, 540, 580, 600 nm,
and wide band filter 420-580 nm
9.4 Incubator
Microplate type: Honeycomb 2 microplate, 100 wells per plate
Incubating temperature: 1.0 - 60.0 °C, selectable with steps of 0.1 °C. Depends on
ambient temperature (see fig. 4). Bioscreen can go 6 °C below
or 30 °C above ambient temperature (max. 60 °C).
Material: Polystyrene
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9.6 PC Requirements
PC Operating BioScreener software can be used in Windows XP,
Vista or 7.
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240 5590 Filter wheel with 405, 420, 450, 492, 540, 580, 600 and WB (420-580) nm filters
240 0620 Halogen lamp 8V 50W – use ONLY lamps that are pre-tested by Growth Curves Ltd
121 0051 Fuse 2A slow, 10 pcs
121 0091 Fuse 4A slow, 10 pcs
Consumables
PC Software
Manual
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11. Appendix
WARRANTY STATEMENT
Warranty
The products of Growth Curves Ltd. are fully guaranteed against defective parts and
materials including defects caused by poor workmanship for a period of one year from the
date of the purchase.
Growth Curves Ltd. will repair or replace defective parts or materials during the term of
warranty at no extra charge for materials and labor, provided that the products were used
and maintained according to Growth Curves Ltd’s instructions. The warranty is invalid if
the products have been misused or abused. Opening or tampering the product is misuse,
and will void warranty.
For warranty to be effective, the product must have been purchased either directly from
Growth Curves Ltd. or from an authorized Bioscreen C distributor. The guarantee is not
transferable to a third party without prior written approval from Growth Curves Ltd.
Disclaimer:
The seller is not liable for any losses or damage arising out of or in connection with
the use of the product or indirect damages.
12. Appendix
• Please fill in the Service Request completely and include it with the instrument.
Describe the problem in detail. Please use separate appendix, if necessary.
• Fill in the Decontamination declaration form. Have the person responsible for
verifying decontamination sign and date the Certificate of Decontamination AND
the Safety/shipping label.
• Attach Safety/shipping label to the exterior of the packaging.
• Take note and follow shipping laws and regulations.
Safety/shipping label
Fill out and sign the Safety/Shipping Label below. Cut out and affix it to the exterior (outside) of the shipping
carton.
Safety/Shipping Label
TO: Growth Curves Ltd
Telekatu 12
20360 Turku
FINLAND
Name Date
This label must be affixed to the exterior of the packaging!
1. Remove all substances which are dangerous for human health from the
instrument so that the instrument is safe for human to handle.
2. Choose the appropriate decontamination method depending on what substances
(liquids) the instrument has been exposed to.
Decontamination areas
The most important areas to be cleaned are the incubator and the sample compartment.
Clean the incubator thoroughly (some white residue from the Honeycomb plates which is
difficult to clean can remain on the corners after the cleaning). If there has been spillage of
any harmful substances, take the incubator tray up and clean also underneath of the
incubator. The incubator tray is attached with two hexagonal screws on both ends of the
tray (large one on the left side and smaller one on the right side).
The carriage can be manually moved to the left in order to clean the bottom part of the
sample compartment.
Bioscreen C Certificate of Decontamination
This unit is certified as having been reviewed by a competent member of our organization and found to have
been totally decontaminated off all chemical, biological and/or radioactive materials PRIOR to packaging for
shipment.
By signing this form I acknowledge that I have read and understood my responsibilities. I have also read and
understood that I have to attach Safety/shipment label to the exterior of the packaging in order to extradite
the expenses for shipment refusal and returning.
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(Printed Name of Company Representative) (Title)
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(Signature) (Date)
Signature of Radiation Safety Officer is also required when the unit has been used with radioactive
materials.
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(Printed Name) (Signature) (Date)
This page is reserved for your notes:
This User’s Manual covers the use, specifications and maintenance of
Bioscreen C Microbiology Reader.
www.bioscreen.fi
www.growthcurvesusa.com
www.bioscreen.fi
Vuorimiehenkatu 13 A 2, 00140 Helsinki, Finland
Phone: +358-9-687 46 300