Specification 1.00215.0500 Hektoen enteric (HE) agar acc. ISO 21567, FDA-BAM and ref. to ISO 6579 GranuCutt® prime Specification ‘Appearance (cleamess) ‘Appearance (color) pHvalue (25 °C) clear to slightly opalescent bluegreen to bluegrey. 73-77 ‘Growth promotion test in accordance with the current version of DIN EN ISO 11133, Specification ‘Growth (Saimonolia typhimurium ATCC good +4028 (WOCM 00031) Growth (Salmonella entersis ATCC 130768 good (woe 00030) Growth (Shigella flexneri ATCC 12022 0d (wooM 00126), Growth (Shigella flexneri ATCC 29903 00d (woeM 00125), {Growth (Shigola sonnel ATCC 29930 (WDCM good orm) Growth (Proteus mirabils ATCC 29906 weak to good (woe 00023) Growth (Escherichia coli ATCC 25922 weak to good (wooM 09013) Growth (Enterococcus fascalis ATCC 29212 none to woak (woe 09087) colonies (Salmonella typhimurium ATCC blue-green +4028 (WOCM 00031) colonics with black centre, without Precipitation zone Colonies (Salmonella entertcis ATCC 13076 blue-green (woen 00034) colonies wth black centre, without Colonies (Shigella flexneri ATCC 12022 (woe 00126) Colonies (Shigella exneri ATCC 29903 (woeM 00125) Precipitation zone {gr00n and moist Colonies without black centre, without Precipitation zone green and moist Colonies without black contre, without Precipitation zone Merck KGaA, Frankfurter Strate 250, 64293 Darmstadt (Germany): +49 6151 72.0 EMD Millipore Corporation -a subsidiary of Merck KGaA, Darmstadt, Germany 400 Summit Drive, Burlington, MA 01803, USA, Phone +1 (781) 533-6000 Page 1 of 2Specification 1,00215.0500 Hektoen enteric (HE) agar acc. ISO 21567, FDA-BAM and ref. to ISO 6579 GranuCult® prime ‘Colonies (Shigella sonnei ATCC 29980 ‘een and moist (woe 00127) raised colonies vwthout black centre, without precipitation zone Colonies (Proteus mirabis ATCC 29908 green to (woe 00023) buegreen colonies with or without black centre, without precipitation zone Colonies (Escherichia coli ATCC 26922 red to salmon (woe 00013) colonies without black centre, with Precipitation zone Colonies (Enterocacous faecalis ATCC 29212 i growth: salmon (woe 00087) cryellowish Colonies without black contre, without Precipitation zone Incubaton: 22 2 hours at 47 £1 °C aerobic Stefanie Fischer ‘Response iboralan manage qual Conval ‘This document has been produced electronically and is val without a signature, Merck KGaA, Frankfurter Strae 250, 64293 Darmstadt (Germany): +49 6151 72.0 Page 2 of 2 EMD Millipore Corporation -a subsidiary of Merck KGaA, Darmstadt, Germany 400 Summit Drive, Burlington, MA 01803, USA, Phone +1 (781) 533-6000