GENETICS

SCIENCE EDUCATION 324

LABORATORY ACTIVITY 6
Problem Set on Linkage and Recombination

GROUP 4

Leader: Tungkag, Norsaidin A.

Members: Abdul, Moner A.
Akmad, Hasna S.
Felix, Jeron S.
Pando, Saidali P.
Parcasio, Marlouie S.

I. Problem-Solving: Show solutions to every problem and highlight by encircling the final answer.

1. Construct a chromosome map given the crossover frequency below:

Gene Crossover frequency

A-C 30%
B-C 45%
B-D 40%
A-D 25%

A. Determine the gene position in a gene map.

45%
40%
B A D C
25%

30%
 B. Determine the final distance between each gene. Provide the gene map with
final distances. Hint: use subtraction

45%
40%

B A 25 MU D 5 MU C
15 MU 25%

30%

2. IN Drosophila, bar shaped eye (B), scalloped wings (S), cross vein-less wings (W) and
eye color (C) are located on the X chromosome. The recombination frequency of each
gene is indicated in the table below.

Gene Crossover frequency

W-B 2.5%
W-C 3.0%
B-C 5.5%
B-S 5.5%
W-S 8.0%
C-S 11.0%

A. Construct a map for the following data provided above.

11.0%

3.0% 8.0%

C W B S

5.5% 5.5%
3. You are provided with gametes from dihybrid cross of heterozygous individuals:

a+ b+
a+ b+
ab
ab

A. Identify if there is combination in the given gametes above.

Condition Gametes Combination
a+ b+ Parental
a+ b+ Parental
No crossover a b Parental
a b Parental

B. If there is single crossover in cis form, what are the combinations produced. Show
your crossover.

Condition Meiotic tetrad Gametes Combination

a+ b+ a+ b+ Parental
Single crossover:
a+ b+ a+ b Recombinant
Markers in cis
a b a b+ Recombinant
a*b*//ab
a b a b Parental

C. If there is single crossover now in trans form, what are the possible combinations
show your crossover.

Condition Meiotic tetrad Gametes Combination

a+ b a+ b Parental
Single crossover:
a+ b a+ b+ Recombinant
Markers in trans
a b+ a b Recombinant
a*b//ab
a b+ a b+ Parental