ANNEX J

List of Parameters for Each Service Capability

Name of Hospital :_________________________________________________________________________________________________

Address of Hospital :_________________________________________________________________________________________________

Please tick () appropriate box/es:

SERVICE CAPABILITY
Bacteriological Analysis Chemical Analysis Physical Analysis Radiological Analysis
 Total Coliform Test:  Acidity  Mercury  Color  Gross Alpha and Gross
Multiple Tube  Alkalinity  Nitrogen Ammonia  Odor Beta Radioactivity
Fermentation  Aluminum  Nitrogen Nitrate  pH Valve  Radium PPG by
Technique (MTFT)  Arsenic  Nitrogen Nitrite  Specific Conductance Radium (Soluble,
 Fecal Coliform Test:  Barium  Oil and Grease  Taste Suspended and Total)
MTFT  Biochemical Oxygen  Oxygen Dissolved  Temperature  Strontium in Water
 Total Coliform Test: Demand  Pesticide  Turbidity  Total Radioactive
Membrane Filtration  Cadmium  Phenol  Total Radium
Technique (MF)  Calcium  Phosphate  Total, Suspended and
 Fecal Coliform Test:  Chloride  Residue Dissolved
MF  Chloride (Residual)  Selenium
 Gram Stain  Chromium (Hexavalent)  Silica Dissolved
 IMVIC Test  Copper  Silver
 Heterotrophic Plate  Cyanide  Sodium
Count (HPC)  Fluoride  Sulfate
 Free Carbon Dioxide  Sulfide
 Hardness  Sulfite
 Iron  Surfactants
 Lead  Total Organic Carbon
 Magnesium  Total Solids
 Manganese

List of Equipment, Reagent, Laboratory Ware and Materials for Specific Test
Name of Hospital :_________________________________________________________________________________________________
Address of Hospital :_________________________________________________________________________________________________
Bacteriological Analysis
Please tick () appropriate box/es for the test/ method applied:
Test/ Method Equipment
 For All General Bacteriological - Autoclave
Preparation and Procedures - Balances, analytical and top-
loading
- Colony counter
- Hot plate with magnetic stirrer
- Oven sterilizer
- pH Meter
- Refrigerator
- Stove
 Total Coliform Test : Multiple - Bacticinerator or similar flame-
Tube fermentation Technique sterilization device
(MTFT) - Bunsen burner
- Incubator, can be maintained
at 35+ 0.5oC
- Isolation hood, laminar flow
hood or biological safety
cabinet
 Fecal Coliform Tests : MTFT - Bacticinerator or similar flame-
sterilization device
- Bunsen burner
ANNEX K
Reagent/ Media Laboratory Ware and Materials
(Refer to specific microbiological Durham tubes, 8 x 45 mm
-
test methods below) - Erlenmeyer flasks, 250 ml,
500 ml, 1000 ml
- Graduated cylinders, 100 ml,
500 ml, 1000 ml
- Petri dishes, 15 x 100 mm
- Pipets, 10 ml, 1.1 ml
- Reagent bottles, brown and
clear
- Stainless spatulae and spoons
- Test tubes, 13 x 100 mm, 15 x
125 mm, 16 x 150 mm, 25 x
150 mm
- Test tube caps
- Test tubes, screw capped, 16
x 150 mm
- Test tube baskets and racks
- Lauryl Tryptose Broth (LTB) - Inoculating loops
- Brilliant Green lactose bile - Pipets, 10 ml
Broth (BGLB)
- EC Medium (EC) - Inoculating loops
 ANNEX K
Test/ Method Equipment Reagent/ Media Laboratory Ware and Materials
- Isolation hood, laminar flow
hood or biological safety
cabinet
- Water bath Incubator, can be
maintained at 44.5C  0.5
 Total Coliform Test: - Bacticinerator or similar flame- - LES Endo Agar (Endo) - Membrane filters, 0.22 µm
Membrane Filtration sterilization device - M-Endo Medium pore size
Technique (MF) - Bunsen burner - Forceps
- Isolation hood, laminar flow
hood or biological safety
cabinet
- Incubator, can be maintained
at 35+ 0.5oC
- Membrane filtration apparatus,
manifold and vacuum pump
- Microscope, compound
- Water bath Incubator, can be
maintained at 44.5C  0.5
 Fecal Coliform Test: MF - Bacticinerator or similar flame- - M-FC Medium - Membrane filters, 0.22 µm
sterilization device pore size
- Bunsen burner - Forceps
- Isolation hood, laminar flow
hood or biological safety
cabinet
- Incubator, can be maintained
at 35+ 0.5oC
- Membrane filtration apparatus,
manifold and vacuum pump
- Microscope, compound
- Water bath Incubator, can be
maintained at 44.5C  0.5
 Presence-Absence (P-A) - Bacticinerator or similar flame- - LTB - Graduated cylinder
Coliform Test sterilization device - P-A Broth
- Bunsen burner
- Isolation hood, laminar flow
hood or biological safety
 ANNEX K
Test/ Method Equipment Reagent/ Media Laboratory Ware and Materials
cabinet
- Incubator, can be maintained
at 35+ 0.5oC
 Colilert - Bacticinerator or similar flame- - Colilert - 51 well microplate (from the
sterilization device - Colilert 18 manufacturer)
- Bunsen burner - Colilert MW - Test tube rack
- Comparator (from the - Colisure
manufacturer)
- Isolation hood, laminar flow
hood or biological safety
cabinet
- Incubator, can be maintained
at 35+ 0.5oC
- Sealer (from the
manufacturer)
- Ultraviolet light, 366 nm
- Water bath incubator
 Gram Stain - Bacticinerator or similar flame- - Nutrient Agar - Forceps
sterilization device - Crystal Violet - Microscope glass slides
- Bunsen burner - Gram’s Iodine - Staining rack
- Isolation hood, laminar flow - 95% Ethyl Alcohol - Wash bottle
hood or biological safety - Safranin O
cabinet
- Incubator, can be maintained
at 35+ 0.5oC
- Microscope, compound
 IMViC tests - Bacticinerator or similar flame- - Nutrient Agar - Inoculating loops
sterilization device - Tryptone Broth (TB) - Pasteur pipets
- Bunsen burner - MR-VP medium (MRVP)
- Isolation hood, laminar flow - Simmon’s Citrate Agar (SCA)
hood or biological safety - Kovac’s reagent
cabinet - Methyl red solution
- Incubator, can be maintained - Barritt’s reagent (a naphthol
at 35+ 0.5oC solution)
- O’Meara’s reagent (KOH
solution)
 ANNEX K
Test/ Method Equipment Reagent/ Media Laboratory Ware and Materials
 Heterotrophic Plate Count - Bacticinerator or similar flame- - Phosphate Buffer Solution - Petri dishes
(HPC) sterilization device (PBS) - Pipets, 10 ml., 1.1 ml, 1.0 ml
- Bunsen burner - 0.1% Peptone water - Bent-glass rod (spreader), if
- Colony counter - Plate Count Agar (PCA) spread plate techniques is
- Isolation hood, laminar flow - Tryptic Soy Agar (TSA) used
hood or biological safety
cabinet
- Incubator, can be maintained
at 35+ 0.5oC
- Vortex mixer
 ANNEX K
List of Equipment, Reagent, Laboratory Ware and Materials for Specific Test

Name of Hospital :_________________________________________________________________________________________________

Address of Hospital :_________________________________________________________________________________________________

Chemical Analysis (Inorganic Chemical Constituent With Health Significance)

Please tick () appropriate box/es for the constituent used:

Laboratory Ware and

Constituent Test/ Method Equipment Reagent/ Media
Materials
 Antimony (Sb) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Standard Sb - Hotplate
- Calibration standards - Fume hood
- QC standards
 Arsenic (As) - ICP - ICP For Hydride Generation AAS/ - For ICP/ EAAS: Argon
- Hydride Generation AAS - Hydride Generation AAS EAAS: - For ICP/ AAS/ EAAS:
- EAAS - EAAS - Nitric Acid Reaction Cell
- Silver - For SDCC: - Hydrochloric Acid - For SDCC: Arsine generator
Diethyldithiocarbamate Spectrophotometer - Standard As - Hotplate
Method (SDDC) - Calibration standards - Fume hood
- QC standards
- Sodium Borohydrate
- Sodium Iodide
- Potassium Persulfate
- Perchloric Acid
For SDDC:
- Acetic Acid
- Sodium Acetate
- Sodium Borohydride
solution
- Hydrochloric Acid
- Lead Acetate
- Silver
Diethyldithiocarbamate
- Standard As
 Barium (Ba) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Standard Ba - Hotplate
- Calibration standards - Fume hood
- QC standards
 Boron (B) - ICP - ICP For ICP: - For ICP: Argon
- Carmine/ Curcumine - Spectrophotometer - Nitric Acid - Hotplate
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
Method - Hydrochloric Acid - Fume hood
- Standard B
- Calibration standards
- QC standards
For Carmine/ Curcumine:
- Hydrochloric Acid
- Standard B
- Sulfuric
 Cadmium (Cd) - FAAS - FAAS For FAAS/ EAAS/ ICP/ ASV: - For FAAS: Acetylene Gas
- EAAS - EAAS - Nitric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Hydrochloric Acid - Hotplate
- Anodic Stripping - For ASV: Electrochemical - Standard Cd - Fume hood
Voltammetry (ASV) analyzer - Calibration standards - For ASV: Electrodes and
- Dithizone Method - For Dithizone: - QC standards cells
Spectrophotometer For ASV:
- Nitrogen high purity
- Electrolyte buffer
(Phosphate/ Citrate)
- Mercury
- Mercuric Nitrate
- Reference electrode filling
solution
- Amalgamated Zn
- Hydrochloric Acid
- Vanadous Chloride
- HF
- Methanol
- Siliconizing solution
- Alumina grits
For Dithizone
- Standard Cd
- Sodium Potassium Tartrate
- Sodium Hydroxide
- Potassium Cyanide
- Hydroxylamine HCl
- Dithizone
- Chloroform
- Tartaric Acid
- Hydrochloric Acid
- Thymol Blue
- Sodium Hydroxide
 Chromium - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
- ICP - ICP - Standard Cr - Hotplate
- Colorimetric - Spectrophotometer - Calibration standards - Fume hood
- QC standards
- Sulfuric Acid
- Phosphoric Acid
- Methyl Orange
- Hydrogen Peroxide
- Ammonium Hydroxide
- Potassium Permanganate
- Sodium Azide
- Diphenylcarbazide
- Chloroform
- Cupferron
 Cyanide - Titrimetric - For Colorimetric: - Sodium Hydroxide - Cyanide distillation
- Colorimetric Spectrophotometer - Magnesium Chloride apparatus
- CN Selective Electrode - For CNSE: Expanded scale - Sulfuric Acide - For Titrimetric/ CNSE: Koch
(CNSE) pH meter or specific ion - Lead Carbonate Microburette
meter - Sulfamic Acid - For CNSE: Cyanide-ion-
For Titrimetric: selective electrode
- P-
dimethylaminobenzalrhodan
ine
- Acetone
- Standard Silver Nitrate
- Potassium Dichromate
For Colorimetric:
- Chloramine –T
- Standard CN
- Barbituric Acid
- Pyridine
- Sodium Acetate
- Sodium Hydroxide
For CNSE:
- Standard CN
- Sodium Hydroxide
- Potassium Nitrate
 Fluoride - Ion chromatography (IC) - IC - Standard Fluoride - For ISE: Fluoride electrode
- Ion-selective electrodes - For ISE: Expanded scale For IC:
(ISE) pH meter or specific ion - Eluent solution
- SPADNS meter - Regenerate solution
- Complexone Method - For SPADNS: For ISE:
Spectrophotometer - Glacial Acetic Acid (HAc)
- For Complexone: Fluoride - Sodium Chloride
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
manifold - Cyclohexylenediaminetetra
Acetic Acid (CDTA)
- Sodium Hydroxide
For SPADNS:
- Zyrconyl Chloride
For Complexone:
- Sulfuric Acid
- Sodium Acetate
- Glacial Acetic Acid
- Alizarin Fluorine
- Ammonium Hydroxide
- Lanthanum Nitrate
- Tertiary Butanol
- Polyoxyethylene 23 lauryl
ether
 Lead (Pb) - FAAS - FAAS - Standard Lead - For FAAS: Acetylene Gas
- EAAS - EAAS - Calibration standards - For EAAS/ ICP: Argon
- ICP - ICP - QC standards - Hotplate
- Anodic Stripping - For ASV: Electrochemical For FAAS/ EAAS/ ICP/ ASV: - Fume hood
Voltammetry (ASV) analyzer - Nitric Acid - For ASV: Electrodes and
- Dithizone Method - For Dithizone: - Hydrochloric Acid cells
Spectrophotometer For ASV:
- Nitrogen high purity
- Electrolyte buffer
(phosphate/Citrate)
- Mercury
- Mercuric Nitrate
- Reference electrode filling
solution
- Amalgamated Zn
- Hydrochloric Acid
- Vanadous chloride
- HF
- Methanol
- Siliconizing solution
- Alumina grits
For Dithizone:
- Nitric Acid
- Ammonium Hydroxide
- Ammonium Citrate
- Sodium Sulfite
- Potassium Cyanide
- Hydroxylamine HCl
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
- Dithizone
- Chloroform
- Iodine
- Potassium Iodide
 Total Mercury (Hg) - Cold vapor AAS - Cold vapor AAS - Standard Hg
- ICP/ MS - ICP/ MS - Calibration standards
- QC standards
For Cold vapor AAS:
- Nitric Acid
- Potassium Permanganate
- Potassium Persulfate
- Sodium Chloride
- Hydroxylamine
sulfate/chloride
- Stannous Chloride
- Hydrochloric Acid
- Sulfuric Acid
 Nickel (Ni) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Standard Ni - Hotplate
- Calibration standards - Fume hood
- QC standards

 Nitrate (NO3-) - Nitrate Electrode Method
(NEM)
- Cd Reduction Method
(CRM)
- Ion chromatography (IC)
- For NEM: pH meter, - Standard Nitrate For NEM:
expanded-scale For NEM: - Double –junction reference
- For CRM: - Aluminum Sulfate electrode
Spectrophotometer - Silver Sulfate - Nitrate Ion electrode
- IC - Boric Acid For CRM:
- Sulfamic Acid - Reduction column
- Sodium Hydroxide
For CRM:
- Cd granules
- Hydrochloric Acid
- Copper Sulfate
- Color reagent: phosphoric
acid, sulfanilamide, N-(1-
naphthyl)-ethylenediamine
dihydrochloride
- Ammonium Chloride
- EDTA
For IC:
- Eluent solution
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
- Regenerant solution
 Nitrite (NO2-) - Colorimetric (Diazotization) - For Colorimetric: - Standard Nitrite
- Ion Chromatography (IC) Spectrophotometer For Colorimetric:
- IC - Phosphoric acid
- Sulfanilamide
- N-(1-naphthyl)-
ethylenediamine
dihydrochloride
For IC:
- Eluent solution
- Regenerant solution
 Selenium (Se) - Hydride Generation AAS - Hydride Generation AAS - Selenium standard - For EAAS/ ICP: Argon
- EAAS - EAAS - Calibration standards - For AAS/ EAAS/ ICP:
- ICP - ICP - QC standards Reaction Cell
- Colorimetric - For Colorimetric: For Hydride Generation AAS/ - Hotplate
- Fluorometric Spectrophotometer EAAS: - Fume hood
- For Fluorometric: - Nitric Acid For Colorimetric/ Fluorometric:
Fluorometer - Hydrochloric Acid - Water bath
- Standard As - pH meter
- Sodium Borohydrate - Centrifuge
- Sodium Iodide - Shaker
- Potassium Persulfate
- Perchloric Acid
For Colorimetric/ Fluorometric:
- HCL
- Ammonium Hydroxide
- Cyclohexane
- 2,3 Diaminonaphthalene
(DAN) solution
- Hydroxylamine EDTA
- Disodium EDTA
 ANNEX K
List of Equipment, Reagent, Laboratory Ware and Materials for Specific Test

Name of Hospital :_________________________________________________________________________________________________

Address of Hospital :_________________________________________________________________________________________________

Chemical Analysis (Organic Chemical Constituent)

Please tick () appropriate box/es for the constituent used:

Laboratory Ware and

Constituent Test/ Method Equipment Reagent/ Media
Materials
 Volatile Organic - GC/PID - GC/PID - Standard solution - Purge and Trap system or
Compounds (e.g. benzene, - GC/ELCD - GC/ELCD - Internal standards/surrogate Headspace autosampler
toluene, chloromethane, - GC/MS - GC/MS standards LLE
chlorobenzene, etc.); - Calibration standards - Drying column
Volatile Aromatic; Volatile - QC standards - Kuderna-Danish set up
Halocarbons; 1,2 (EDB) - Sample prep/extraction - Vials
Dibromoethane; 1,2 supplies SPE
Dibromo-3- Chloropropane LLE - SPE column
(DBCP) - Sodium Hydroxide - Sample concentrator
- Sodium Sulfate Gas
- Sodium Thiosulfate - Nitrogen gas*
- Sulfuric Acid - Hydrogen gas*
- Acetone - Helium gas*
- Methanol
- Methylene Chloride * gases depend on the
SPE equipment
- Appropriate solvent
 Trihalomethanes and - GC/MS - GC/MS LLE - Sample vials
Chlorinated Organic - GC-ECD - GC-ECD - Extraction Solvent - Microsyringes
Solvents (pentane, hexane, MTBE) - Extraction vessel
- Methyl Alcohol - Specific columns
- Neat Standard materials – - Mechanical Shaker
Calibration standards Gas
- Internal Standard - Nitrogen gas*
- Stock Standards - Hydrogen gas*
- QC standards - Helium gas*
- Calibration standards
* gases depend on the
equipment

Constituent
 Disinfection By Products: - GC-ECD
Haloacetic Acids and
Trichlorophenol
 Disinfection by Products: - PFBHA LLE GC
Aldehydes
 Polynuclear Aromatic - GCMS
Hydrocarbons, Phthalates,
ANNEX K
Laboratory Ware and
Test/ Method Equipment Reagent/ Media
Materials
- GC-ECD LLE - Sample vials
- Extraction Solvent (MTBE) - Microsyringes
- Methanol, pesticide grade - Extraction vessel
- Sodium Sulfate - Specific columns
- Ammonium Chloride - Mechanical Shaker
- Reagents for Diazomethane - Volumetric flask, 2,5, 10 ml
generation
- Diethylene glycol
monoethylether
- N-methyl-N-nitroso-p-
toluene sulfonamide
- Ethyl Ether, absolute
- Potassium Hydroxide
- Standard materials
- Internal Standard
- Surrogate Standards
- Calibration Standards
- QC standards
- Silica Gel
- Sulfuric Acid
- Copper Sulfate
pentahydrate
- GC-ECD - Hexane, UV grade - Sample containers and
- Methanol extraction vials: 40 ml screw
- Ammonium Chloride top
- Sulfuric Acid - Microsyringe
- Organic free reagent water - Volumetric flask, 5, 10, 25
- Buffer pH 6: Potassium ml
hydrogen phthalate, Sodium - Syringe: 20 ml, hypodermic
Hydroxide - Automatic pipette dispenser
- Derivatizing agent: PFBHA - Water bath or incubator
(2,3,4,5,6 - Pasteur pipette
pentafluorobenzyl-
hydroxylamine
hydrochloride
- Aldehyde standard
- Internal standard
- Surrogate standard
- Calibration standard
- QC standard
- GCMS - Sodium Hydroxide solution, LLE
10 N - Drying column

Constituent
Phenolics
 Polychlorinated biphenyls
 Organochlorine pesticides
ANNEX K
Laboratory Ware and
Test/ Method Equipment Reagent/ Media
Materials
- Sodium Sulfate - Kuderna-Danish set up
- Sodium Thiosulfate - Vials
- Sulfuric Acid, 1+1 - Separatory funnel, 2 L
- Acetone, Methanol, - Drying column,
Methylene Chloride, chromatographic
pesticide quality - Water bath
- Stock standard solution - Continuous liquid extractor
- Calibration standards
- QC standards
- GCMS GCMS GCMS LLE
- GC-ECD - Sodium Hydroxide solution, - Drying column
10 N - Kuderna-Danish set up
- Sodium Sulfate - Vials
- Sodium Thiosulfate - Separatory funnel, 2 L
- Sulfuric Acid, 1+1 - Drying column,
- Acetone, Methanol, chromatographic
Methylene Chloride, - Water bath
pesticide quality - Continuous liquid extractor
- Standard solution GC-ECD LLE
- Calibration standards - Drying column
- QC standards - Kuderna-Danish set up
GC-ECD - Vials
- Hexane - Separatory funnel, 2 L
- Petroleum Ether - Drying column,
- Diethyl Ether chromatographic
- Ethyl Acetate - Water bath
- Methylene Chloride - Continuous liquid extractor
- Magnesia-silica gel - Glass wool
- Sodium Sulfate
- Silanized glass wool
- Carrier gas: Nitrogen and
Argon Methane
- PCB reference material
- Calibration standards
- QC standards
- GC-ECD GC-ECD GC-ECD LLE
- GCMS - Hexane - Drying column
- Petroleum Ether - Kuderna-Danish set up
- Diethyl Ether - Vials
- Ethyl Acetate - Separatory funnel, 2 L
- Methylene Chloride - Drying column,
- Magnesia-silica gel chromatographic
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
- Sodium Sulfate - Water bath
- Silanized glass wool - Continuous liquid extractor
- Carrier gas: Nitrogen and - Glass wool
Argon Methane GCMS LLE
- OCP reference material - Drying column
- Calibration standards - Kuderna-Danish set up
- QC standards - Vials
GCMS - Separatory funnel, 2 L
- Sodium Hydroxide solution, - Drying column,
10 N chromatographic
- Sodium Sulfate - Water bath
- Sodium Thiosulfate - Continuous liquid extractor
- Sulfuric Acid, 1+1
- Acetone, Methanol,
Methylene Chloride,
pesticide quality
- Stock standard solution
- Calibration standards
- QC standards
 Carbamate Pesticides - HPLC - HPLC equipped with - Methanol - Micro-filter
column - Sodium Hydroxide, 0.05 N
- 2-Mercaptoethanol solution
- Sodium Borate
- OPA reaction solution (0-
phthalaldehyde)
- Monochloroacetic acid
buffer
- Stock pesticide solution
- Calibration standards
- QC standards
- Sample bottle, 120 ml screw
cap polypropylene
 Acid Herbicide Compound - GC-ECD - GC-ECD (Micro Liquid- - 5 MTBE - Sample and extraction vials,
Liquid Extraction GC) - Sodium Sulfate 40 or 60 ml screw cap vials,
- Methanol TFE silicon
- Reagents for diazomethane - Microsyringe
generation - Syringe, 30 ml
- Sodium Hydroxide - Microvolumetric flask: 2, 10
- N-methyl-N-nitroso-p- ml
toluene sulfonamide - Mechanical shaker
- Silica gel - Extract and standard
- Sulfuric Acid solution storage container:

Constituent Test/ Method
 Glyphosate Herbicides - Liquid Chromatographic
Post-Column Fluorescence
ANNEX K
Laboratory Ware and
Equipment Reagent/ Media
Materials
- Copper Sulfate 1.8 ml clear glass, 14 ml
pentahydrate amber glass screw cap vials
- Analytical standards with TFE-lines silicone
- Calibration standards septa
- QC standards - Transfer pipette (Pasteur
pipette)
- Salt scoop
- Diazomethane generator
- pH strips
- HPLC w/ fluorescence - Phosphoric Acid - Filter, 0.45 um
detector - Sulfuric Acid - Round bottom flask, 500 ml
- Hydrochloric Acid - Rotary evaporator
- Potassium Dihydrogen - Nitrogen gas for drying
Phosphate - Filter paper
- Disodium Ethylenediamine
Tetraacetic Dihydrate
(EDTA)
- Oxidation Reagent:
- Sodium Chloride
- Sodium Hydroxide
- Calcium Hypochlorite
- Pot Dihydrogen Phosphate
- Fluorogenic labeling
reagent
- 0-phthaldehyde
- 2-mercaptoethanol
- Boric Acid powder
- Potassium Hydroxide
- Glyphosate analytical
standards
- Amino Methylphosphoric
Acid analytical standard
- Glyphosate and AMPA
fortification standard
- Glyphosate and AMPA
HPLC calibration standard
 ANNEX K
List of Equipment, Reagent, Laboratory Ware and Materials for Specific Test

Name of Hospital :_________________________________________________________________________________________________

Address of Hospital :_________________________________________________________________________________________________

Chemical Analysis (Disinfection and other Disinfection by Products)

Please tick () appropriate box/es for the constituent used:

Laboratory Ware and

Constituent
 Chlorine (Cl2) Residual
 Chlorine (Cl2) Residual
Test/ Method
- Iodometric Method
- Amperometric Titration
- DPD Ferrous Titration
Equipment Reagent/ Media
Materials
For Iodometric: For Amperometric:
- Acetic Acid, conc - End point detection
- Potassium Iodide apparatus: cell unit
- Standard Sodium connected to micrometer
Thiosulfate 0.1 N with reference electrode
- Potassium Bi-iodate or - Agitator
Potassium Iodate or
K2Cr2O7 Starch indicator
- Iodine
For Amperometric:
- Standard phenylarsine
oxide titrant: w/ Sodium
Hydroxide, Hydrochloric
Acid, starch
- Phosphate buffer solution,
pH 7: Potassium
Dihydrogen Phosphate,
Disodium Hydrogen
Phosphate, Sodium
Hypochlorite, Chlorine,
Potassium Iodide
- Acetate buffer: Sodium
Acetate, conc. Acetic Acid
For DPD Ferrous Titration:
- Phosphate buffer solution:
Potassium Dihydrogen
Phosphate, Disodium
Hydrogen Phosphate,
EDTA, Mercuric Chloride
- N, N-Diethyl-p-
phenylenediamine (DPD)
oxalate w/ Sulfuric Acid,
EDTA

Constituent Test/ Method
 Chlorine (Cl2) Residual - DPD Colorimetric Method
- Syringaldazine
- Iodometric Electrode
Technique
Equipment
- For DPD Colorimetric/
Syringaldazine: Photometer/
Spectrophotometer
ANNEX K
Laboratory Ware and
Reagent/ Media
Materials
- Standard Ferrous
ammonium sulfate titrant: w/
Sulfuric Acid, Phosphoric
Acid, Barium Diphenylamine
Sulfonate, Potassium
Dichromate
- Potassium Iodide
- Sodium Arsenite solution
- Thioacetamide
- Glycine solution
- Barium Chloride
For DPD Colorimetric: For Syringaldazine:
- Phosphate buffer solution: - Ultrasonic agitator
Potassium Dihydrogen - 30.5 cm Vigerux column
Phosphate, Disodium For Iodometric Electrode:
Hydrogen Phosphate, - Platinum and Iodide ion-
EDTA, Mercuric Chloride selective electrode
- N, N-Diethyl-p-
phenylenediamine (DPD)
oxalate: w/ Sulfuric Acid,
EDTA
- Standard Ferrous
ammonium sulfate titrant: w/
Sulfuric Acid, Phosphoric
Acid, Barium Diphenylamine
Sulfonate, Potassium
Dichromate
- Potassium Iodide
- Sodium Arsenite solution
- Thioacetamide
- Chlorine demand free water
For Syringaldazine:
- Chlorine demand free water
- 2- propanol
- Buffer: Potassium
Dihydrogen Phosphate,
Disodium Hydrogen
Phosphate
- Hypochlorite solution
For Iodometric Electrode:
- pH 4 buffer solution:
Sodium Acetate, conc.
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
Acetic Acid
- Chlorine demand free water
- Standard Potassium iodate
 Iodine (I2) - Leuco Crystal Violet Method - For Leuco Crystal Violet: - Iodine demand free water For Amperometric:
- Amperometric Titration Photometer/ - Standard Iodine - End point detection
Spectrophotometer - Standard Sodium apparatus: cell unit
Thiosulfate connected to micrometer
- Potassium Iodide with reference electrode
- Standard Sodium - Agitator
Thiosulfate 0.1 N
- Potassium Bi-iodate or
Potassium Iodate or
Potassium Dichromate
- Starch indicator
For Amperometric:
- Standard phenylarsine
oxide titrant: w/ Sodium
Hydroxide, Hydrochloric
Acid, starch
- Acetate buffer: Sodium
Acetate, conc. Acetic Acid
 Cyanogen Chloride - Colorimetric - Spectrophotometer - Chloramine –T - Cyanide distillation
- Standard CN apparatus
- Barbituric Acid
- Pyridine
- Sodium Acetate
- Sodium Hydroxide
- Sodium Chloride
- Sodium Carbonate
- Sulfuric Acid
- EDTA
- Formaldehyde
- Phosphate buffer: Sodium
dihydrogen phosphate
monohydrate
 ANNEX K
List of Equipment, Reagent, Laboratory Ware and Materials for Specific Test

Name of Hospital :_________________________________________________________________________________________________

Address of Hospital :_________________________________________________________________________________________________

Chemical Analysis (Physical and Chemical Quality for Acceptability Aspects)

Please tick () appropriate box/es for the constituent used:

Laboratory Ware and

Constituent Test/ Method Equipment Reagent/ Media
Materials
 Color - Visual comparison Method - Spectrophotometer - Potassium Chloroplatinate - Nessler tubes 100 ml, short
- Spectrophotometric Method - Cobaltous Chloride form
- Sodium Hydroxide - Filtration apparatus
- Sulfuric Acid
 Turbidity - Nephelometric Method - Nephelometer/Turbidimeter - Turbidity standards
 Acidity - Titration Method - Carbon dioxide free water
- Potassium Hydrogen
Phthalate
- Sodium Hydroxide
- Phenolphthalein
 Alkalinity - Titration Method - Sodium Carbonate
- Standard Sulfuric Acid, 0.02
N
- Methyl Red indicator
- Bromcresol Green indicator
 Hardness - EDTA Titrimetric Method - Buffer Solution:
- Ammonium Chloride
- Ammonium Hydroxide
- EDTA
- Complexing agent:
- Sodium Hydroxide,
Sodium Cyanide
- Sodium Sulfide
nonahydrate
- Magnesium salt of 1,2
cyclohexane
diaminetetraacetic acid
(MgCDTA)
- Indicators: Eriocrome black
T or Calmagite
- Standard EDTA 0.01 M
 Conductivity - Conductivity meter
 Total Solids - Gravimetric - Drying Oven - Evaporating Dish
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
- Dessicator
 Total Dissolved Solids - Gravimetric - Drying Oven - Evaporating Dish
- Dessicator
- Glass fiber filter
- Filtration apparatus
 Total Suspended Solids - Gravimetric - Drying Oven - Evaporating Dish
- Dessicator
- Glass fiber filter
- Filtration apparatus

 Aluminum (Al) - FAAS - FAAS
- EAAS - EAAS
- ICP - ICP
- Eriochrome Cyanide R - For Eriochrome Cyanide R:
Spectrophotometer
 Chloride (Cl-) - Argentometric Method - For Potentiometric Method:
- Potentiometric Method Electronic voltmeter
- Automated Ferricyanide - Mechanical stirrer
Method - For Automated Ferricyanide
- Ion Chromatography Method: Automated
analytical equipment
- Ion Chromatograph
- Nitric Acid - For FAAS: Acetylene Gas
- Hydrochloric Acid - For EAAS/ ICP: Argon
- Standard Al - Hotplate
- Calibration standards - Fume hood
- QC standards - For Eriochrome Cyanide R:
For Eriochrome Cyanide R: Nessler tubes, 50 ml
- Sulfuric Acid
- Ascorbic Acid
- Buffer reagent: Sodium
Acetate, Acetic Acid
- Stock dye solution:
Solochrome Cyanide or
Eriochrome Cyanide R
- Methyl Orange
- EDTA
- Sodium Hydroxide
For Argentometric Method: - For Potentiometric Method:
- Potassium Chromate Glass and silver-silver
- Standard Silver Nitrate: chloride electrode
0.0141 N - For Automated Ferricyanide
- Standard Sodium Chloride: Method: Filters 480 nm
0.0141 N
For Potentiometric Method:
- Standard Sodium Chloride,
0.0141 N
- Nitric Acid, conc.
- Standard Silver Nitrate,
0.0141 N
- Pretreatment reagents:
Sulfuric Acid (1+1),
Hydrogen Peroxide, Sodium
Hydroxide, 1 N
For Automated Ferricyanide
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
Method:
- Color Reagent:
- Stock Mercuric Thiocyanate
- Stock Ferric Nitrate
- Polyxylene 23 lauryl ether
- Standard chloride solution
For Ion Chromatography:
- Eluent solution: Sodium
bicarbonate
- Regenerant solution:
Sulfuric Acid, 0.025 N
- Standard Chloride solution
 Copper (Cu) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Standard Cu - Hotplate
- Neocuproin Method - For Neocuproin Method/ - Calibration standards - Fume hood
- Bathocuproin Method Bathocuproin Method: - QC standards - For Neocuproin Method:
Spectrophotometer/ Filter For Neocuproin Method: Separatory funnel, 125 ml
photometer - Redistilled water, copper - For Bathocuproin Method:
free Nessler tubes 100 ml tall
- Standard Cu form
- Sulfuric Acid, conc. - Acid washed glassware
- Hydroxylamine
hydrochloride
- Sodium Citrate
- Chloroform
- Ammonium Hydroxide
- Congo red paper
- Methanol
- Nitric Acid, conc.
- Hydrochloric Acid, conc.
For Bathocuproin Method:
- Redistilled water, copper
free
- Standard Cu
- Hydroxylamine
Hydrochloride
- Sodium Citrate
- Disodium Bathocuproin
Disulfonate solution
- Chloroform
- Hydrochloric Acid, conc
 Iron (Fe) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Standard Fe - Hotplate
- Phenanthroline Method - For Phenanthroline Method/ - Calibration standards - Fume hood
- Bathocuproin Method Bathocuproin Method: - QC standards - For Phenanthroline Method:
Spectrophotometer/ Filter For Phenanthroline Method: Nessler tubes 100 ml tall
photometer - Standard Fe form
- Hydroxylamine - Acid washed glassware
Hydrochloride - Separatory funnel, 125 ml
- Sodium Acetate
- Phenanthroline
- Ammonium Acetate buffer:
w/ glacial acetic acid
- Disopropyl or isopropyl
ether
- Hydrochloric Acid, conc.
For Bathocuproin Method:
- Redistilled water, copper
free
- Standard Cu
- Hydroxylamine
Hydrochloride
- Sodium Citrate
- Disodium Bathocuproin
Disulfonate solution
- Chloroform
- Hydrochloric Acid, conc
 Manganese (Mn) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Standard Mn - Hotplate
- Persulfate Method - For Persulfate Method: - Calibration standards - Fume hood
Spectrophotometer/ Filter - QC standards - For Persulfate Method:
photometer For Persulfate Method: Nessler tubes 100 ml tall
- Standard Mn form
- Special reagent: Mercuric
Sulfate
- Nitric Acid, conc.
- Silver Nitrate
- Hydrogen Peroxide
- Ammonium Persulfate
- Phosphoric Acid
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
 Sodium (Na) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- ICP - ICP - Hydrochloric Acid - For EAAS/ ICP: Argon
- Standard Sodium - Hotplate
- Calibration standards - Fume hood
- QC standards
 Zinc (Zn) - FAAS - FAAS - Nitric Acid - For FAAS: Acetylene Gas
- EAAS - EAAS - Hydrochloric Acid - For EAAS/ ICP: Argon
- ICP - ICP - Standard Zn - Hotplate
- Dithizone Method I - For Dithizone Method I: - Calibration standards - Fume hood
- Dithizone Method II Spectrophotometer/ Filter - QC standards - For Dithizone Method I:
photometer For Dithizone Method I: Separatory funnel, 125 ml
- pH meter - Zinc free, water - Nessler tubes
- Standard Zn - Acid washed glassware
- Sodium Acetate
- Acetic Acid, 1+7
- Sodium Thiosulfate
- Stock Dithizone
- Carbon Tetrachloride
- Sodium Citrate
- Hydrochloric Acid, conc.
For Dithizone Method II:
- Zinc free, water
- Standard Zn
- Methyl Red
- Acetic Acid, conc.
- Ammonium Hydroxide
- Potassium Cyanide
- Stock Dithizone
- Carbon Tetrachloride
- Sodium Citrate
- Bis (2-hydroyetthyl
dithiocarbamate solution)
diethanolamine, Carbon
Disulfide, Methanol
- Sodium Sulfide
- Nitric Acid, 6 N
- Hydrogen Sulfide
 Zinc (Zn) - Zincon Method - Spectrophotometer/ Filter For Zincon Method:
photometer - Zinc free, water
- pH meter - Standard Zn
- Sodium Ascorbate
- Sodium Hydroxide

Constituent Test/ Method
 Silica - Gravimetric
- Molybdosilicate Method
- Heteropoly Blue Method
 Sulfide (S-) - Methylene Blue
- Gas Dialysis, Automated
Methylene Blue Method
(ask for the diagram)
- Iodometric Method
- Ion Selective Electrode
Method
Equipment
- For Molybdosilicate
Method / Heteropoly Blue
Method:
Spectrophotometer/ Filter
photometer
- For Methylene Blue:
Spectrophotometer/ Filter
photometer
- For Gas Dialysis,
Automated Methylene Blue
Method: Automated
Analytical Equipment
- For Ion Selective Electrode
Method: pH meter
- Magnetic Stirrer
ANNEX K
Laboratory Ware and
Reagent/ Media
Materials
- Boric Acid
- Zincon reagent in methanol
- Cyclohexanone, purified
- Hydrochloric Acid
- Potassium Cyanide
- Hydrochloric Acid, 1+1, - Platinum crucible with
1+50 covers
- Sulfuric Acid, 1+1 - Platinum evaporating dish,
- Hydrofluoric Acid, 48% 200 ml
- Perchloric Acid, 72%
For Molybdosilicate Method:
- Sodium Bicarbonate
- Sulfuric Acid, 1N
- Hydrochloric Acid 1+1
- Ammonium Molybdate
- Oxalic Acid
- Standard Silica
For Heteropoly Blue Method:
- Sodium Bicarbonate
- Sulfuric Acid, 1N
- Hydrochloric Acid 1+1
- Ammonium Molybdate
- Oxalic Acid
- Standard Silica
- 1-amino-2-naphthol-4-
sulfonic acid
- Sodium Sulfite
- Sodium Bisulfite
For Methylene Blue: - For Ion Selective Electrode
- Amine Sulfuric Acid stock Method: Silver/sulfide
solution: N, N dimethyl-p- electrode
phenylenediamine oxalate, - Double junction reference
Sulfuric Acid electrode
- Amine Sulfuric Acid reagent - Electrode polishing
- Ferric Chloride - Strip
- Diammonium Hydrogen - Electrochemical cell
Phosphate - Gas dispersion tube
- Methylene Blue
- Sodium Sulfide
For Gas Dialysis, Automated
Methylene Blue Method:
- N, N dimethyl-p-
phenylenediamine

Constituent Test/ Method
 Sulfate (SO4-2) - Ion Chromatographic
Method
- Gravimetric Method with
Ignition of Residue
- Gravimetric Method with
Drying of Residue
- Turbidimetric
ANNEX K
Laboratory Ware and
Equipment Reagent/ Media
Materials
dihydrochloride
- Hydrochloric Acid, 6 n
- Ferric Chloride
- Sodium Hydroxide, 0.01 N
Standard Sulfide (Sodium
Sulfide
- Zinc Acetate
For Iodometric Method:
- Hydrochloric Acid, 6 N
- Standard Iodine solution,
0.0250 N
- Potassium Iodide
- Standard Sodium
Thiosulfate, 0.0250N
- Starch solution
For Ion Selective Electrode
Method:
- Alkaline antioxidant
reagent: Sodium Hydroxide,
Ascorbic Acid, Disodium
EDTA
- Lead Perchlorate (0.1M)
- Sulfide standard (Sodium)
- Ion Chromatograph For Ion Chromatographic For Gravimetric Method with
- For Gravimetric Method Method: Ignition of Residue/ Gravimetric
with Ignition of Residue/ - Eluent solution: Sodium Method with Drying of Residue:
Gravimetric Method with Bicarbonate-Sodium - Evaporating Dish
Drying of Residue: Drying Carbonate - Dessicator
Oven - Regenerant solution: - Acid washed filter paper or
- Drying Oven Sulfuric Acid, 0.025 N Membrane filter, 0.45 um
- For Turbidimetric: - Standard Sulfate - Filtration apparatus
Spectrophotometer/ Filter For Gravimetric Method with For Turbidimetric:
photometer/ Nephelometer Ignition of Residue/ Gravimetric - Stopwatch
- Magnetic stirrer Method with Drying of Residue: - Measuring spoon
- Methyl Red
- Hydrochloric Acid, 1+1
- Barium Chloride solution
- Silver Nitrate-Nitric Acid
reagent
- Silicone fluid
For Turbidimetric:
- Buffer solution A:
Magnesium Chloride,
 ANNEX K
Laboratory Ware and
Constituent Test/ Method Equipment Reagent/ Media
Materials
Sodium Acetate, Potassium
Nitrate, Acetic Acid or
- Buffer solution B:
Magnesium Chloride,
Sodium Acetate, Potassium
Nitrate, Sodium Sulfate,
Acetic Acid
- Barium Chloride, crystals,
20 to 30 mesh
- Standard Sulfate
 Sulfate (SO4-2) - Automated Methylthymol - Automated analytical - Barium Chloride solution - Ion Exchange column
Blue Method (ask for the equipment - Methylthymol Blue reagent
diagram) with Barium Chloride
solution, 1 N HCl, 95%
ethanol
- Buffer solution: Ammonium
Chloride, Ammonium
Hydroxide
- EDTA reagent
- Sodium Hydroxide, 0.36 N
- Standard sulfate
 pH - Electrometric Method - pH meter - pH standards: 4, 7, 10

Note: For analytes/parameters not included in the list and with PNSDW (2007) standards being performed by the laboratory, the list of
equipment, reagents and laboratory supplies shall be submitted to NRL-EAMC for evaluation.

 FAAS – Flame Atomic Absorption Spectrometry

 EAAS – Electrothermal Atomic Absorption Spectrometry
 GC – Gas Chromatography
 ECD – Electron Capture Detector
 MS – Mass Spectrophotometer
 PID – Photoionization Detector
 HPLC – High-performance Liquid Chromatography
 ICP – Inductively Coupled Plasma
 ANNEX K
 IC – Ion Chromatography
 PFBHA – Pentafluorobenzyl-hydroxylamine
 PNSDW – Philippine National Standards for Drinking Water (2007)

Reference: Standard Methods for the Examination of Water and Wastewater, 19 th and 20th ed.