Professional Documents
Culture Documents
Identification of Medically Important Bacteria
Identification of Medically Important Bacteria
Chocolate agar
• Loeffler’s
coagulated
serum:
growth
of
Corynebacterim
diphtheria
and
produc%on
of
metachroma:c
granules
Buffered charcoal
yeast extract agar
with Legionella Enrichment
media:
contain
specific
colonies nutrients
required
for
the
growth
of
a
par%cular
pathogen
• used
to
enhance
the
growth
of
a
par%cular
pathogen
from
a
mixture
of
organisms
by
taking
advantage
of
its
nutri%on
specificity
TCBS agar
• MacConkey
sorbitol
agar:
selec%on
and
differen%a%on
of
E.coli
O157:H7
in
stool
specimens
• EHEC
(E.coli
O157:H7)
does
not
ferment
sorbitol
Pink lactose Pale non lactose
fermenting colonies fermenting colonies
Differen3al
media:
contain
some
nutrient
or
chemical
which
allows
colonies
of
bacterial
species
or
type
to
exhibit
certain
metabolic
or
culture
characteris%cs
which
can
be
used
to
dis%nguish
them
from
other
bacteria
in
the
same
plate
Cagulase + S.aurues
Gram positive cocci
clusters chains
Staphylococci Streptococci
Staphylococci
Streptococci
Catalase test
positive negative
Staphylococci Streptococci
coagulase
positive negative
Staphylococcus aureus Staphylococcus epidermidis/
saprophyticus
Streptococci
streptococci Growth on blood agar identification
Gamma hemolysis,
Enterococcus spp., Beta hemolysis,
Streptococcus pyogenes
Streptococcus agalactiae
Gram positive cocci in chains
Beta hemolysis
Group A? group B?
CATALASE
negative
PYR POSITIVE
PYROGLUTAMYL
Alpha hemolysis, Streptococcus pneumoniae,?
S.mutans ? Viridans streptococci
Optochin resistant
S.mitis
Optochin sensitivity
S.pneumoniae
Alpha hemolysis
Beta hemolysis on blood agar
on blood agar
Streptococcus Viridans
pneumoniae streptococci
Optochin
Group A or Group B
Bile solubility
Sensitive resistant
soluble insoluble
Group A Group B
Streptococcus Viridans
pneumoniae streptococci
Enterococcus
• No hemolysis on
blood agar
• 40% bile tolerance
• Grow in 6.5%
sodium chloride
Gamma hemolysis,
Enterococcus spp.
GRAM NEGATIVE COCCI
Growth on
Thayer Martin
medium
Fermentation of sugars
Neisseria gonorrhoea
Neisseria meningitidis
Gram negative cocci in pairs
Oxidase test
positive
Neisseria
Klebsiella pneumoniae
Gram negative bacteria; A lactose fermenting; (E.coli or Klebsiella)
B. non lactose fermenting colonies (Salmonella or Proteus or
Pseudomonas or Shigella on Mac Conkey’s medium
Lactose fermenting Gram negative bacilli
E.coli ??
Klebsiella ??
Indole test
Oxidase test
Oxidase positive
Oxidase negative
Pseudomonas spp;
Salmonella spp;
Vibrio cholerae
Shigella spp;
Proteus spp;
Oxidase negative: PROTEUS AND
SALMONELLA
Urease +ve
swarming
TSI
• Fermentation of glucose,
lactose, and/or sucrose
• Reduction of sulfur to
hydrogen sulfide
• Gas formation
Used for
Enterobacteriaceae
API system for identification
CAMPYLOBACTER COLONIES
GROWN AT 420c MICROAEROPHILIC
Motility
H 2S
No H2S
producer
Salmonella Shigella
Gram positive rods
Aerobic Gram positive rods
Bacillus anthracis Listeria monocytogenes
Anaerobic rods;
Bacteroides fragilis
Gram negative cocco
bacilli with fastidious
growth requirements
Haemophilus influenzae
Culture performed using chocolate
agar
COLONIES ON
LOWENSTEIN
JENSEN MEDIUM