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2000 - Sharma Et Al. - Oral Pharmacokinetics of Omeprazole and Lansoprazole After Single and Repeated Doses As Intact Capsules or As Sus
2000 - Sharma Et Al. - Oral Pharmacokinetics of Omeprazole and Lansoprazole After Single and Repeated Doses As Intact Capsules or As Sus
problems in our studies, we subsequently evaluated the by the institutional review board of the University of
effectiveness of omeprazole and lansoprazole as suspen- Arkansas for Medical Sciences, Little Rock, AR.
sions in 8.4% sodium bicarbonate.1, 2 These were All studies were performed in the Division of Gastro-
termed, respectively, simpli®ed omeprazole suspension enterology, University of Arkansas for Medical Sciences,
and simpli®ed lansoprazole suspension. The per-gas- Little Rock, AR. In a pre-determined random order,
trostomy administration of simpli®ed lansoprazole sus- subjects received each of four proton pump inhibitor
pension raised intragastric pH to levels that were formulations once daily for 5 days. The dosing periods
comparable to those seen with the intact capsule were separated by 9-day washout periods. The four
formulation of lansoprazole.5 However, simpli®ed omep- formulations studied were intact omeprazole capsules
razole suspension administered through a gastrostomy 20 mg, simpli®ed omeprazole suspension 20 mg, intact
had an effect on intragastric pH that was quantitatively lansoprazole capsules 30 mg and simpli®ed lansoprazole
smaller than that observed with either intact omepraz- suspension 30 mg. These were taken at 08.00 hours on
ole capsules or intact, non-encapsulated omeprazole each day, 30 min before the ®rst food of the day and
granules in orange juice.6 after an overnight fast. Study drugs were administered
Little is known about the absorption of either omep- in this way to mimic, as far as was reasonably possible,
razole or lansoprazole when administered as a suspen- the manner in which proton pump inhibitors are
sion in sodium bicarbonate. In one study, an oral usually prescribed.
suspension of lansoprazole granules in a ¯avouring On the ®rst and ®fth days of dosing, subjects had serial
solution produced comparable plasma levels to those blood sampling for plasma drug levels. An intravenous
seen with the administration of intact lansoprazole cannula was inserted into an arm vein and 10 mL blood
capsules.7 The administration of intact lansoprazole samples were withdrawn at 0, 0.5, 1, 1.5, 2, 2.5, 3 and
granules orally in apple sauce, or via a nasogastric tube 6 h after dosing for, as appropriate, plasma omeprazole
in apple juice, produces plasma lansoprazole levels that or lansoprazole levels. Subjects were observed during
are comparable to those seen with the intact capsule.8, 9 these times in a specialized clinical investigation unit.
The absorption of omeprazole from its standard capsule Subjects had a standard breakfast 30 min after dosing
formulation displays marked inter- and intra-individual and had nothing further by mouth until the conclusion
variability.10 Furthermore, the proportion of the omep- of the study. Blood samples were collected into tubes
razole dose that is absorbed increases with repeated containing heparin sodium and centrifuged at
once daily administration to reach a plateau by the 5th 2500 r.p.m. for 15 min. Plasma was decanted and
day.10±12 However, the bioavailability of lansoprazole samples stored at )20 °C until being shipped in batch at
does not progressively increase with repeated daily the conclusion of the study to Harris Laboratories
dosing, being near maximal after a single dose.13, 14 (Lincoln, NE) for analysis.
We conducted this study to compare the absorption Lansoprazole (internal standardÐomeprazole) and
characteristics of each proton pump inhibitor when omeprazole (internal standardÐlansoprazole) were
administered after single and repeated once-daily dos- extracted from heparinized plasma into ethyl ether:
ing, as intact capsules and as suspensions in sodium methylene chloride, 70:30 (v:v). The extracts were dried
bicarbonate. under nitrogen and reconstituted in acetonitrile and
injected into an on-line LC/MS/MS system. Detection of
the analytes was performed with an Inertsil silica
MATERIALS AND METHODS
column and a Perkin-Elmer API 365 tandem mass
Twelve healthy women were recruited for the study. spectrometer using a turbo ion spray interface. Positive
Their mean ( s.d.) age was 36.8 ( 9.8) years, their ions (m/z of 370.2±> 251.9 for lansoprazole and
mean ( s.d.) weight was 73.6 ( 12.7) kg and their 346.2±> 198.1 for omeprazole) were monitored in the
mean ( s.d.) height was 1.66 ( 0.07) m. None had MRM mode.
any signi®cant past medical history of note and none We have previously described the methods for the
was taking any regular prescribed medication. None preparation of simpli®ed omeprazole suspension and
had received either omeprazole or lansoprazole in the simpli®ed lansoprazole suspension.5, 6 Brie¯y, these
preceding month. Each subject gave her written, were prepared by opening a 20-mg omeprazole capsule
informed consent to the study, which was approved or a 30-mg lansoprazole capsule, pouring the granular
contents into 10 mL of 8.4% sodium bicarbonate the suspensions and capsules were compared using the
(pH 7.8; osmolality 20 mOsm/mL) and gently mixing paired Student's t-tests. All tests were two-tailed;
until the contents were suspended. The resulting milky P-values of 0.05 or lower were considered statistically
white liquid was then administered by mouth. The signi®cant.
suspensions were freshly prepared for each administra-
tion during this study. The pH of simpli®ed omeprazole
RESULTS
suspension was 7.97; the pH of simpli®ed lansoprazole
suspension was 7.92. Each subject completed all four dosing schedules. No
Using a 10-cm visual analogue scale, subjects were adverse events were reported during dosing with
asked to rate the palatability of each of the four omeprazole or lansoprazole, either as intact capsules
formulations administered on day 1 and day 5. On this or as suspensions. For both omeprazole and lansopraz-
scale, zero indicated the worst imaginable taste and 10 ole, each subject preferred the intact capsule formula-
the best. tion to the liquid suspension in sodium bicarbonate
(P < 0.001 for each comparison).
Statistical analysis
Omeprazole and simpli®ed omeprazole suspension
Plasma omeprazole and lansoprazole levels were tabu-
lated for each subject. Maximum plasma concentration Table 1 lists the summary pharmacokinetic data for
(Cmax) and time to Cmax (tmax) were derived from omeprazole when given as intact capsules and as
inspection of the data. The area under the plasma simpli®ed omeprazole suspension. There was no signi-
concentration/time curve (AUC) was calculated accord- ®cant change in tmax between days 1 and 5 of dosing
ing to the linear trapezoidal rule. with either omeprazole capsules or simpli®ed omepraz-
Data for tmax, Cmax and AUC were summarized as ole suspension. However, tmax on simpli®ed omeprazole
medians and ranges, and analysed non-parametrically. suspension was signi®cantly lower than on omeprazole
Values for tmax, Cmax and AUC with omeprazole capsules capsules at day 1 (P 0.03) but not at day 5
were compared to those with simpli®ed omeprazole (P 0.075). During dosing with omeprazole capsules,
suspension using the Wilcoxon matched-pairs signed- Cmax and AUC were signi®cantly higher on day 5 than
rank test. Corresponding data for lansoprazole and on day 1 (P 0.041 and 0.003, respectively). How-
simpli®ed lansoprazole suspension were handled simi- ever, there was no signi®cant difference for Cmax or
larly. Omeprazole data were not compared with lan- AUC, comparing days 1 and 5 of simpli®ed omeprazole
soprazole data. Data for tmax, Cmax and AUC for each suspension administration (P 0.388 and 0.093,
formulation on day 1 of its administration were respectively).
compared with those on day 5 of administration. The On day 5, Cmax after standard omeprazole capsules was
relative bioavailability of omeprazole from simpli®ed signi®cantly higher than with simpli®ed omeprazole
omeprazole suspension and of lansoprazole from suspension (P 0.034), although no signi®cant differ-
simpli®ed lansoprazole suspension was de®ned as ence was seen on day 1 (P 0.158). AUC was higher
AUCsuspension/AUCcapsule..7 Data on palatability between after standard omeprazole capsules than after simpli®ed
Table 1. Summary pharmacokinetic data expressed as median (range) following omeprazole 20 mg o.d. as capsules and as simpli®ed
omeprazole suspension
Table 2. Summary pharmacokinetic data expressed as median (range) following lansoprazole 30 mg o.d. as capsules and as simpli®ed
lansoprazole suspension
in AUC seen between days 1 and 5 of dosing with lansoprazole suspension had a profound and consistent
simpli®ed lansoprazole suspension (Table 2). By day 5, effect on intragastric acidity.5 Since lansoprazole is well
the bioavailability of lansoprazole from simpli®ed lan- absorbed when given as simpli®ed lansoprazole suspen-
soprazole suspension was similar to that seen with the sion, further study of this formulation in appropriate
capsule formulation. clinical situations is certainly justi®ed.
Phillips et al. have previously studied simpli®ed omep-
razole suspension in the prevention of stress-related ACKNOWLEDGEMENT
gastric bleeding in critically ill patients in an intensive
care unit.15 They administered two 40 mg doses of Financial support for this study was provided by TAP
simpli®ed omeprazole suspension on the ®rst day Pharmaceuticals Inc., Deer®eld, IL, USA.
followed by a daily 20 mg dose for the duration of their
study. These patients were not comparable to those in REFERENCES
our previous pharmacodynamic study of simpli®ed
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