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Virology Introductionby Shyamal
Virology Introductionby Shyamal
Lecture
Dr Shyamal Kr Paul
Assoc. Prof.
Microbiology
MMC
What is a virus?
Basic structure of a virus?
Classification of virus?
What is the principle of viral replication?
What are the steps of viral replication?
Define – capsomere, capsid, virion, envelop
Atypical virus like particels- Prions, viroid,
pseudovirion, defective virus
infected!
The virus concept.
In 1892, Dmitri Iwanowski, a Russian botanist,
showed that extracts from diseased tobacco
plants could transmit disease to other plants
after passage through ceramic filters fine
enough to retain the smallest known bacteria.
Generally recognised as the beginning of
Virology.
virion
capsid
capsomeres
nucleic acid
envelope
DNA or RNA
Single stranded or double stranded
Sense (+) or antisense (-) nucleic acid
Linear or circular genome
Intact or segmented nucleic acid
Non-enveloped viruses:
They are relatively stable and resistant to environment
insult i.e. they can resist desiccation, dry heat and soap
They generally kill the cells they infect as part of their life
cycle i.e. cell lysis
Enveloped viruses:
Can shed from infected cells in long term chronic
infection. This is called budding
Envelopes are usually derived from the host cell
membrane
The envelope is relatively sensitive to desiccation, dry
heat and detergents
1/4/2014 Dr. Shyamal Kr Paul, Virology introduction 22
Viral replication
During penetration, the viral envelope fuses with host cell membrane
and nucleocapsid enters the host cell. This is followed by uncoating
during which the viral capsid is enzymatically degraded and the viral
genome
1/4/2014 Dr. Shyamal Kr Paul, Virology introduction 29
Enveloped Virus Replication