Objective

PPale temporary mount of human cheek epithelial

a

record observations and draw their cells, and to study its characteristics, to
labelled diagrams.
Materials Requireda
ACIean Spatula or a toothpick, a brush. a
stain, a
glycerine, a compound microscope. needle. slide, cover slip, blotting paper, methylene Diue
Theory
Like plant cells, animal cell is also the
structural and functional unit of living organisms. However, cell
wall is absent in animal cells.
The outermost protective
animal cell comprises cell covering of an animal cell is a cell membrane. An
membrane, nucleus, cytoplasm and other cell organelles.
Cheek cells are
squamous epithelium. The epithelial tissue is the outermost
and cavity of an animal body. Cheek cells are thin and flat
covering of most organs
pavement.
Procedure
1. Rinse your mouth with fresh but warm water to remove
any food particles.
2. Gently scrap the inner side of your cheek with the help of a toothpick or a clean and sterilized
spatula.
3. Keep the scrapped material into a drop of water.
4. Spread the scrappings, uniformly with the help of needle.
5. Add a drop of methylene blue stain. Put a drop of glycerine over it after about 3-minutes.
6.
6. Gently place the coverslip with the help of needle over the slide to avoid entry of air bubbles.
7. Remove the excess glycerine from the edges of coverslip with the help of blorting paper.
8. Observe the slide first under low power and then high power of microscope.
9. Draw diagrams of cells as seen.

Observations
a) Observation under low power of microscope:
and compactly arranged. Cell
epithelial cells are seen. These are smaller, polygonal shape
in
Everal
membrane boundaries are irregular.
 - Cell membrane

- Nucleus

-Cytoplasm

Fig.13. Check cells of Human

(b) Observation under high power of microscope

Cell membrane is distinct. It comprises a dense cytoplasm and a distinct nucleus.

Results and Discussion

Cheek cells are epithelial tissue. These are small, polygonal and compactly arranged. Cells have
irregular cell membrane as outermost boundaries. Each cell encloses a distinct nucleus and a vacuole.

Precautions
1. Scrap the cheeks gently to avoid injury.
2. Scrappings should be thin and uniform.
3. Avoid the excess or under staining of the cheeks cells.
4. Avoid the entry of air bubbles while mounting the coverslip.