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Photosynthesis Short Concepts
Photosynthesis Short Concepts
Photosynthesis Short Concepts
NCERT SOLUTIONS
EXEMPLER PROBLEMS
2020
32 YEARS OF NEET MCQS
PHOTOSYNTHESIS
BIOLOGY ASSIGNMENT
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Chapter–13
PHOTOSYNTHESIS IN HIGHER PLANTS
MIND MAP
NCERT SOLUTIONS
-:IMPORTANT POINTS:-
1) Green plants carry out 'photosynthesis' a physio-chemical process by which
they use light energy to drive the synthesis of organic compounds.
2) Photosynthesis in plants requires chlorophyll, light and CO2
3) Joseph Priestley (1733-1804) in 1770, performed experiments to find out the
role of oxygen in growth of green plants.
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18) In light reaction, pigments are associated into two discrete photochemical
Light Harvesting Complexes (LHC) within the Photosystem I (PS-I) and
Photosystem II (PS-II).
19) Each photosystem has all the pigments (except one molecule of
chlorophyll-α) forming a light harvesting system also called antennae.
20) The single chlorophyll-α molecule forms the reaction center.
21) In PIS-I, the reaction center chlorophyll-α has an absorption peak at 700
nm, hence is called P700, while in PSS-II, it has absorption maxima at 680 and
called P680
22) In PS-II, the chlorophyll-α absorbs 680 um wavelengths of red Light causing
electrons to become excited and jump into an orbit,
23) These electrons are picked up by an electron acceptor, which passes them
Co an electrons transport system consisting of cytochromes.
24) These electrons are not used up but are passed on to the pigments of PS-I,
25) The electrons in the reaction center PS-I are also excited when they receive
red light of wavelength 700 mm and are transferred to another acceptor
molecule.
26) These electrons move downhill to a molecule of energy rich NADP+. The
addition of these electrons reduces NADP+ to NADPH+H+.
27) This whole scheme of transfer of electrons, starting from PS-II, uphill to the
acceptor and finally down hill to NADP+ causing it to reduced to NADPH+H+. Is
called Z-Scheme, due to its characteristic shape.
Cyclic Photophosphorylation
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29) The process by which ATP is synthesized by cells (in mitochondria and
chloroplasts) is called Photophosphorylation.
30) Photophosphorylation is the synthesis of ATP from ADP and inorganic
phosphate in the presence of light. It can be of two types.
a) Non-Cyclic Photophosphorylation – occurs when two photosystems work
in a series. PS-II and then PS-I
b) Cyclic Photophosphorylation occurs when only PS-I is functional. The
electron is circulated within the photosystem and cyclic flow of electrons
occurs. It occurs in stroma, lamella and when wavelength of beyond 680
nm is available.
31) The cyclic flow results only in synthesis of ATP but of NADPH +H+
32) Chemiosmotic hypothesis explains the mechanism of ATP synthesis in
chloroplast. ATP synthesis is related to development of a proton gradient
across a membrane.
33) The steps that cause a proton gradient to develop are:
i. The proton or hydrogen ions that are produced by splitting of water,
accumulate within the lumen of thylakoids
ii. As electrons move through the photosystems, protons are transported
across the membrane. This is because the primary acceptor of electron,
which is located towards the outer side of membrane transfers its electrons
not to an electron carrier but to H carrier. Hence, this molecule removes a
proton from the stroma, while transporting an electron. The proton is then
released into the inner side of the membrane.
iii. The NADP reductase enzyme is located on the stroma side of membrane.
The protons are necessary for the reduction of NADP+ to NADPH + H+.
These protons are also removed from the stroma.
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34.A proton gradient develops when within the chloroplast protons in the stroma
decrease in number, while in the lumen there is accumulation of protons.
35.The proton gradient is necessary as the breakdown of gradient releases
energy.
36.The breakdown of gradient provides enough energy to cause a change in F l
particle of the ATPase, which makes the enzyme synthesize several molecules
of energy packed ATP. ATPase enzyme catalyzes the formation of ATP.
37.The ATP is used immediately in biosynthetic reaction taking place in stroma,
responsible for fixing CO2 and synthesis of sugar.
38.Biosynthetic phase or dark reaction does not directly depend on the presence
of light but 'is dependent on the products of light reaction, i.e., ATP and
NADPH, besides CO2and H2O.
39.CO2 assimilation during photosynthesis follow two routes:
• C3-pathway, in which first product of CO2 fixation is a C3 acid (PGA-3
phosphoglyceric acid).
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The Calvin cycle proceeds in three stage: (I) carboxylation during which
CO2 Combines with ribulose-l , 5-bisphosphate: (2) reduction, during
which carbohydrate is formed at the expenses of the photochemically
made ATP and NADPH: and (3) regeneration during which the CO2
accepter ribulose-l,
5-bisphosphate is formed again, so that the cycle continues,
45.For every CO2 molecule entering the Calvin cycle, 3 molecules of ATP and two
of NADPH are required. To make one molecule of glucose 6 turns of cycle are
required.
46.C4-pathway is present is plants that are adapted to live in dry tropical regions.
47.These plants (C4-plants) have the C4 oxaloacetic acid as the first CO2 fixation
product, they use the C3-pathway or the Calvin cycle as the main biosynthetic
pathway.
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48.Characteristics of C4-plants:
i. They have a special type of leaf anatomy.
ii. They can tolerate high temperatures.
iii. They show a high light intensity.
iv. They lack a process called photorespiration and have greater productivity
of biomass.
49. C4-plants have bundle sheath cells in their leaves called as 'Kranz anatomy'.
50. In Kranz anatomy, bundle sheath cells form several layers around the
vascular bundles, having a large number of chloroplasts, thick walls
impervious to gas exchange and no intercellular spaces.
51. C4-plants follow Hatch and Slack pathway. In this, the primary acceptor is a
3-carbon molecule Phosphoenol Pyruvate (PEP) and is present in
mesophyll cells. The enzyme used is PEP carboxylase.
52. The mesophyll cells lack RuBisCO enzyme. The C4-acid OAA is formed in the
mesophyll cells.
53. OAA then forms other 4-carbon compounds like malic acid or aspartic acid
in the mesophyll cells itself.
54. The 3-carbon molecule released is transported back to mesophyll cells
where it is converted to PEP again, thus completing the cycle.
55. The CO2 released in bundle sheath cells now enters the C3 or the Calvin
pathway (common to all plants), Calvin cycle does not take place in
mesophyll cells in (C4-plants but occurs only in bundle sheath cells,
56. Photorespiration is also a process, which creates an important difference
between C3 and C4 plants
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iii. In this pathway, there is neither synthesis of sugars nor of ATP. Rather, it
results in release of CO2 with the utilization of ATP. So, photorespiration is
a wasteful process here.
58. In C4-plants, photorespiration does not occur, this is because they have the
mechanism that increases the concentration of CO2 at the enzyme site.
i. This takes place C4-acid from mesophyll cells is broken down in the bundle
sheath cells to release CO2, this results in increasing the intracellular
concentration of CO2
ii. In turn, this ensures that the RuBisCO functions as a carboxylase minimizing
the oxygenase activity.
iii. The (C4-plants lack photorespiration, that is why the productivity and fields
are better in these plants.
59. Photosynthesis is under the influence of both internal (plant) and external
factors.
i. Internal factors are the number, size, age and orientation of leaves,
mesophyll cells and chloroplasts, internal CO2 concentration and the
amount of chlorophyll.
ii. External factors are availability of sunlight temperature, CO2 concentration
and water.
60. Black man's law of limiting factors state that 'if a chemical process is
attached by more than one factor, then its rate will be determined by the
factor, which is nearest to its minimal value. It is the factor, which directly
affects the process, if its quantity is changed.
61. Light as a limiting factor:
i. At higher intensities, gradually the rate does not show further increase as
other factors become limiting.
ii. Light saturation occurs at 10% of the full sunlight.
iii. Increase in incident light beyond a point causes the breakdown Of
chlorophyll and a decrease in photosynthesis.
62. Carbon dioxide as a limiting factor;
i. The C3 and C4-plants respond differently to CO2 concentrations.
ii. At high light intensities, both C3 and C4-plants, show increase in the rates of
photosynthesis, Hence, C4-plants show saturation at about 360/µlL , while
C3 responds to increased CO2 concentration acting as a limiting factor.
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iii. This is the reason C3 plants like tomatoes and bell pepper are allowed to
grow in CO2 enriched atmosphere that leads to high yield.
63. Temperature as a limiting factor:
i. The C4-plants respond to higher temperatures and show higher rate of
photosynthesis, while C3-plants have a much lower optimum temperature.
64. Water stress causes stomata to close hence, reducing the CO2 availability.
NCERT Exercises
1) By looking at a plant externally can you tell whether a plant is C3 or C4? Why
and how?
Answer Usually plants growing in dry conditions use C4-pathways. It cannot be
said conclusively, if the plant is a C4 or C4 buy looking at external appearance.
2) By looking at which internal structure of a plant can you tell whether a plant
is C3 or C4? Explain.
Answer The particularly large cells around the vascular bundles of the CA-
pathway plants are called bundle sheath cells and the leaves, which have such
anatomy are said to have 'Kranz anatomy. 'Kranz' means 'wreath' and is a
reflection of the arrangement of cells, the bundle sheath cells may form several
layers around the vascular bundles; they are characterized by having a large
number of chloroplasts, thick walls impervious to gaseous exchange and no
intercellular spaces.
3) Even though a very few cells in a C4,-plant carry out the biosynthetic-
Calvin pathway, yet they are highly productive. Can you discuss why?
Answer C4-plants chemically fix carbon dioxide in the cells of the mesophyll by
adding it to the three-carbon molecule phosphoenolpyruvate (PEP), a reaction
catalyzed by an enzyme called PEP carboxylase. It creates the four-carbon organic
acid, oxaloacetic acid. Oxaloacetic acid or malate synthesized by this process is
then translocated to specialized bundle sheath cells where the enzyme, RuBisCO
and other Calvin cycle enzymes are located and where CO2 released by
decarboxylation of the four-carbon acids is then fixed by RuBisCO activity to the
three-carbon sugar 3-phosphoglyceric acids.
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6. Why is the colour of a leaf kept in the dark frequently yellow or pale green?
Which pigment do you think is more stable?
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7. Look at leaves of the same plant on the shady side and compare it with the
leaves on the sunny side. Or, compare the potted plants kept in the sunlight
with those in the shade. Which of them has leaves that are darker green? Why?
Answer The leaves of the same plant on the sunny side are dark green as
compare it with the leaves on the sunny side.
8. The following figure shows the effect of light on the rate of photosynthesis.
Based on the graph, answer the following questions
i. At which point/s (A, B or C) in the curve is light a limiting factor?
ii. What could be the limiting factor/s in region A?
iii. What do C and D represent on the curve?
Answer
i. Points B-C of the curve, the rate did not increase with an increase in its
concentration, because light becomes a limiting factor under these
conditions.
ii. In A region, the rate of photosynthesis shows proportionate increase up to
a certain CO2 concentration, beyond, which the rate again becomes
constant.
iii. Points C and D represent that if the light intensity is doubled, CO 2
concentration again becomes limiting factor beyond this concentration.
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Chlorophyll does not receive any electrons The source ot electrons is photolysis ot
5 from donor, water.
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The system does not take part in This system is connected with C02 fixation
6 photosynthesis expect in bacteria, and is dominant in green plants,
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Question 8. ATPase enzyme consists of two parts. What are those parts? How
are they arranged in the thylakoid membrane? Conformational change occur in
which part of the enzyme?
Answer ATPase enzyme consists of two parts, i.e., a stalk and a headpiece. These
two parts are located completely across the inner mitochondrial membrane. The
conformational changes occur in the headpiece of this enzyme.
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oxygen from carbon dioxide and water, In cellular respiration cells make ATP
(which is used as energy by the cells) and consume oxygen during the conversion
of glucose to carbon dioxide and water.
Question 4. If a green plant is kept in dark with proper ventilation, can this plant
carry out photosynthesis? Can anything be given as supplement to maintain its
growth or survival?
Answer It would die because plants need sun light for photosynthesis. The sun is
the only thing that will help plants make their own food. If there is a small
opening in the room that let in sunlight however, the plant would grow towards
the opening or the light to get it's food.
Question 6. In tropical rain forests, the canopy is thick and shorter plants growing
below it, receive filtered light. How are they able to carry out photosynthesis?
Answer The light filtering through the canopy of broad-leaved trees is richer in
red waves and poorer in blue-violet radiations. Red rays induce maximum growth
and development in leaves and stems. Blue-violet radiations keep the growth
under check but the plant organs are otherwise normal
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Question 9. Find out how Melvin Calvin worked out the complete biosynthetic pathway for the
synthesis of sugar.
Answer Calvin discovered the 'Calvin cycle' by choosing a suitable organism to work with a
single-celled alga called Chlorella, devising a Claver piece of apparatus and by applying two
carefully-chosen techniques.
The first technique he used was to track the path of carbon during photosynthesis by using a
radioactive isotope, 14 C.
The second technique was two-dimensional paper chromatography By this technique, the
separated the substances, which the had extracted from the cells.
At the end, he was able to describe the sequence of compounds through which the carbon
passed as it was converted from carbon dioxide to carbohydrate. He discovered that it was in
fact a cycle of reactions, hence the name 'Calvin cycle'.
Question 10. Six turns of Calvin cycle are required to generate one mole of glucose.
Explain.
Answer Calvin cycle is a series of reactions that lead to the formation of glucose.
The steps involve utilization of 2 molecules of ATP for phosphorylation and two
of NADPH for reduction per CO2 molecule fixed. The fixation of six molecules of
CO2 and six turns of the cycle are required for the removal of one molecule of
glucose from the pathway.
Hence, for every CO2 molecule entering the Calvin cycle, 3 molecules Of ATP and
2 of NADPH are required. It is probably to meet this difference in number of ATP
and NADPH used in the dark reaction that the cyclic phosphorylation takes place.
To make one molecule of glucose six turns Of the cycle are required.
Question 11. Complete the flow chart for cyclic photophosphorylation of the photosystem-l.
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Answer
Question 12. In what kind of plants do you come across 'Kranz' anatomy? To
which conditions are those plants better adapted? How are these plants better
adapted than the plants, which lack this anatomy?
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Answer C4-plants have Kranz anatomy. These plants are better adapted to dry
tropical regions. These plants are better adapted than plants which lack this
anatomy (Kranz anatomy) because
i. They can tolerate high temperatures.
ii. They show a response to highlight intensities.
iii. They lack a process called photophosphorylation and have greater
productivity of biomass.
Question 13. A process is occurring throughout the day, in X organism. Cells are
participating in this process. During this process, ATP, CO2 and water are evolved.
It is not a light dependent process.
i. Name the process.
ii. Is it a catabolic or an anabolic process?
iii. What could be the raw material for this process?
Answer
I. Respiration
II. It is a catabolic process
III. Glucose, fat or protein.
Question 14. Tomatoes, carrots and chilies are red in color due to the presence of
one pigment. Is it a photosynthetic pigment?
Answer: Lycopene pigment, which is a chromoplast. It is a non-photosynthetic
pigment.
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Question 17. A cyclic process is occurring in C3-plant, which is light dependent and
needs O2. This process doesn't produce energy rather it consumes energy.
i. Can you name the given process?
ii. Is it essential for survival?
iii. What are the end products of this process?
iv. Where does it occur?
Answer
i. Photorespiration.
ii. No, it is not essential for survival,
iii. Hydrogen peroxide
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Question 18. Suppose Euphorbia and maize are grown in the tropical area.
i. Which one of them do you think will be able to survive under such
conditions?
ii. Which one of them is more efficient in terms of photosynthetic activity?
iii. What difference do you think are there in their leaf anatomy?
Answer Euphorbia will be able to survive under such conditions.
Maize is more efficient in terms of photosynthetic activity,
The maize is a C4-plants have Kranz anatomy type of leaf anatomy, The bundle
sheath -cells are characterized by having a large number of chloroplasts, thick
walls impervious to gaseous exchange and no intercellular spaces, In Euphorbia,
which undergoes CAM (Crassulacean Acid Metabolism) both pathways occur in
mesophyll cells only they do not have Kranz anatomy. Their stomata remain
closed during the day.
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Question 4. In the figure given below, the black line (upper) indicates action
spectrum for photosynthesis and the lighter line (lower) indicates the
absorption spectrum of chlorophyll-a, answer the followings
What does the action spectrum indicate? How can we plot an action spectrum?
Explain with an example.
How can we derive an absorption spectrum for any substance?
If chlorophyll-a is responsible for light reaction of photosynthesis, why do the
action spectrum and absorption spectrum not overlap?
Answer
The effectiveness of different wavelengths of light on photosynthesis is
measured and plotted the amount of action. This is called the action spectrum
of photosynthesis.
Absorption of different wavelengths of light by a particular pigment is plotted
and is called the absorption spectra of that pigment.
Chlorophyll-a is responsible for light reaction of photosynthesis, but the action
spectrum and absorption spectrum do not overlap because though chlorophyll
is the main pigments responsible for absorption of light, other thylakoid
pigments like chlorophyll-b. xanthophylls and carotenoids, which are called
accessory pigments also absorb and transfer the energy to chlorophyll-a.
Indeed, they not only enable a wider range of wavelength of incoming light to
be utilized for photosynthesis but also protect chlorophyll-a from photo-
oxidation,
Question 5. What are the important events and end products of the light
reaction?
Answer-Light reaction or the photochemical phase include light absorption.
Water splitting, oxygen release and the formation of high-energy chemical
intermediaries, ATP and NADPH, The pigments are organized in Photo system I
(PS-I) and photosystem-II (PS-II). The pigments help to make Photosynthesis more
efficient by absorbing different wavelengths of light.
Products of light reaction (cyclic and non-cyclic) are ATP, NADPH2 and O2.The
oxygen is liberated from the green Plants. ATP and NADPH2 are used in dark
reaction for the reduction of CO2 to form carbohydrates, Hence, the ATP and
NADPH2 the products of light reaction are called assimilatory power,
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Question 10. Why is RuBisC0 enzyme the most abundant enzyme in the world?
Answer The enzyme ribulose-l, 5-bisphosphate carboxylase/oxygenase, most
commonly known by the shorter name RuBisCO is used in the Calvin cycle to
catalyse the first major step of carbon fixation. RuBisCO is thought to be the most
abundant protein in the world since it is present in every plant that undergoes
photosynthesis and molecular synthesis through the Calvin cycle. It makes about
20-25% of the soluble protein in leaves and is made on the Earth at the rate of
about 1000 kg/s. It is estimated that every person on Earth is supported by about
44 kg of RuBisCO.
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Answer In C4-plants, photorespiration does not occur. This is because they have
a mechanism that increases the concentration of CO2 at the enzyme site. This
takes place when the C4-acid from the mesophyll is broken down in the bundle
sheath cells to release CO2, This results in increasing the intracellular
concentration of CO2. In turn, this ensures that the RuBisCO functions as a
carboxylase minimizing the oxygenase activity,
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3. A plant in your garden avoids photorespiratory losses, has improved water use
efficiency, shows high rates of photosynthesis at high temperatures and has
improved efficiency of nitrogen utilization, in which of the following physiological
groups would you assign this plant? [NEET 2016, Phase I]
a) C4 b) CAM c) Nitrogen Fixer d) C3
4. Emerson's enhancement effect and red drop have been instrumental in the
discovery of [NEET 2016, Phase I]
a) Two photosystems operating simultaneously
b) Photophosphorylation and cyclic electron transport
c) Oxidative phosphorylation
d) Photophosphorylation and non-cyclic electron transport
6. The oxygen evolved during photosynthesis comes from water molecules. Which
one of the following pairs of elements involved in this reaction? [NEET 2016,P-1]
a) Manganese and chlorine b) Manganese and potassium
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7. The process which makes major difference between CJ3 and C 4-plants is
[NEET2016,Phase II]
(a) glycotysis (b) Calvin cycle (c) photorespiration (d) respiration
14. PGA as the first C02 -fixation product was discovered in photosynthesis of
[CBSE AIPMT 2010]
(a) bryophyte (b) gymnosperm (c) angiosperm (d) alga
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16. Stroma in the chloroplasts of higher plants contains [CBSE AIPMT 2009]
a) light-independent reaction enzymes
b) tight-dependent reaction enzymes
c) ribosomes
d) chlorophyll
20.In the leaves of (C4-plants, malic acid formation during CO2 -fixation occurs in
the cells of [CBSE AIPMT 2007, 08]
a) mesophyll b) bundle sheath c) phloem d) epidermis
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protein
24.As compared to a C4-plant, how many additional molecules of ATP are needed
for net production of one molecule of hexose sugar by C4 a-plants [CBSE
AIPMT 2005]
a) 26 b) 6 c) 12 d) Zero
26. In C3 -plants, the first stable product of photosynthesis during the dark
reaction is [CBSE AIPMT 2004]
a) malic acid b) oxaloacetic acid
c) 3-phosphoglyceric acid d) phosphoglyceraldehyde
27. Plants adapted to low light intensity have [CBSE AIPMT 2004]
a) larger photosynthetic unit size than the sun plants
b) higher rate of CO2 fixation than the sun plants
c) more extended root system
d) leaves modified to spines
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29. Which fractions of the visible spectrum of solar radiations are primarily
absorbed by carotenoids of the higher plants? [CBSE AIPMT 2003]
a) Violet and blue b) Blue and green c) Green and red d) Red and violet
30. In sugarcane plant 14 CO2 is fixed in malic acid, in which the enzyme that fixes
CO2 is [CBSE AIPMT 2003]
a) fructose phosphatase
b) ribulose bisphosphate carboxylase
c) phosphoenol pyruvic acid carboxylase
d) ribulose phosphate kinase
31. Which one of the following is wrong in relation to photorespiration?
[CBSE AIPMT 2003]
a) It is a characteristic of C3-plants
b) It occurs in chloroplasts
c) It occurs in day time only
d) It is a characteristic of C4-plants
32. Which element is located at the centre of the porphyrin ring in chlorophyll?
[CBSE AIPMT 2003]
a) Manganese b) Calcium c) Magnesium d) Potassium
35. Which pigment system is inactivated in red drop? [CBSE AIPMT 2001]
a) PS-I and PS-II b) Ps-I c) PS-II d) None of the above
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37. How many turns of Calvin cycle yield one molecule of glucose? [AIPMT 2000]
a) 8 b) 2 c) 6 d) 4
39. Fixation of one CO2 molecule through Calvin cycle requires [CBSE AIPMT 2000]
a) 1 ATP and 2NADPH2 b) 2 ATP and 2NADPH2
c) 3 ATP and 2NADPH2 d) 2ATP and INADPH2
40. Photochemical reactions in the chloroplast are directly involved in [AIPMT 2000]
a) formation of phosphoglyceric acid
b) fixation of carbon dioxide
c) synthesis of glucose and starch
d) photolysis of water and phosphorylation of ADP to ATP
41. Which enzyme is most abundantly found on earth? [CBSE AIPMT 1990]
a) Catalase b) RuBisCO c) Nitrogenase d) Invoctase
42. Protochlorophyll differs from chlorophyll in lacking [CBSE AIPMT 1998]
a) 2 hydrogen atoms in one of its pyrrole rings
b) 2 hydrogen atoms in two of its pyrrole rings
c) 4 hydrogen atoms in one of its pyrrole rings
d) 4 hydrogen atoms in two of its pyrrole rings
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44. The principle of limiting factors wag proposed by [CBSE AIPMT 1996]
a) Blackmann b) Hill c) Arnon d) Liebig
45. Which one of the following is represented by Calvin cycle? [CBSE AIPMT 1996]
a) Reductive carboxylation b) Oxidative carboxylation
c) Photophosphorylation d) Oxidative phosphorylation
46. Photorespiration is favored by [CBSE AIPMT 1996]
a) high O2 and low CO2 b) low light and high O2
c) low temperature and high O d) low O2 and high CO2
47. Chlorophyll-a molecule at its carbon atom 3 of the pyrrole ring-II has one of
the following [CBSE AIPMT 1996]
a) aldehyde group b) methyl group c) carboxyl group d) magnesium
49. Pigment acting as a reaction centre during photosynthesis is[CBSE AIPMT 1994]
a) carotene b) phytochrome c) P700 d) cytochrome
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53. A photosynthesis in g plant is releasing 18O more than the normal. The plant
must have been supplied with [CBSE AIPMT 1993]
18 18
a) O3 b) H2O with O c) CO2 with O d) C6H12O6 with 18 O
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60. The enzyme that catalyses initial carbon dioxide fixation in Co-plants is
[CBSE AIPMT 1992, 2002]
a) RuBP carboxylase b) PEP carboxylase
c) carbonic anhydrase d) carboxydismutase
63. Photosynthetic pigments found in the chloroplasts occur in [CBSE AIPMT 1991]
a) thylakoid membranes b) plastoglobules
c) matrix d) chloroplast envelope
65. During monsoon, the rice crop of Eastern states of India shows lesser yield
due to limiting factor of [CBSE AIPMT 1997]
a) CO2 b) light c) temperature d) water
66. Which technique has helped in investigation of Calvin cycle? [CBSE AIPMT 1991]
a) X-ray crystallography b) X-ray technique
c) Radioactive isotope technique d) intermittent light
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68. The first carbon dioxide acceptor in C4-plants is [CBSE AIPMT 1990, 92]
a) phosphoenol-pyruvate b) ribulose I ,5-diphosphate
c) oxalo acetic acid d) phosphoolyceric acid
71. A very efficient converter of solar energy with net productivity of 2-4 kg/m 2 or
more is the crop of [CBSE AIPMT 1989]
a) wheat b) sugarcane c) rice d) bajra
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: ANSWER KEYS:
1) b 2) c 3) a 4) a
5) a 6) a 7) c 8) d
9) a 10) d 11) c 12) d
13) b 14) d 15) d 16) a
17) b 18) d 19) c 20) a
21) d 22) c 23) a 24) c
25) d 26) c 27) a 28) C
29) a 30) c 31) d 32) c
33) a 34) b 35) c 36) a
37) c 38) a 39) c 40) d
41) b 42) a 43) b 44) a
45) a 46) a 47) a 48) b
49) c 50) a 51) b 52) b
53) b 54) d 55) b 56) c
57) d 58) c 59) d 60) b
61) b 62) c 63) a 64) a
65) b 66) c 67) a 68) a
69) a 70) b 71) b 72) c
73) d 74) d
2. (c) In C4-plants, the initial fixation of CO2 occurs in mesophyll cells. The primary
acceptor of CO2 is Phosphoenol Pyruvate (PEP). It combines with CO2 in the
presence of enzyme PEP carboxylase to form the first stable product, i.e. Oxalo
Acetic Acid (OAA). Where as C3 -plants lack PEP carboxylase enzyme. They possess
RuBisCO enzyme. This enzyme can work as both carboxylase (fixation of CO2) and
oxygenase (fixation of O2). RuBisCO has a much greater affinity for CO2 than for O2
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and the binding is competitive. At higher temperature, its affinity for CO2
decrease and it works as oxygenase.
Therefore, at higher temperature photosynthesis decrease in C3-plants, while in
C4 -plants it increases.
8. (d) The light-independent reactions (or dark reactions, takes place in the
stromal matrix of the chloroplasts.
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12.(d) CAM plants are mostly succulent xerophytes. The stomata in these plants
remain closed during the day, This helps to check the transpiration, In this
way, water is conserved.
15. (b) C4 -plants are more efficient in photosynthesis than C3-plants but use
more energy. They possess the larger number of chloroplasts in the leaf cells. In
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the leaves of C4 -plants, the vascular bundles are surrounded by bundle sheath
cells which in turn are surrounded by mesophyll cells.
Chloroplast in bundle sheath cells are larger and always contain grana, whereas
chloroplasts in mesophyll cells are smaller.
16. (a) In higher plants, enzymes for light independent reactions (dark reactions)
are present in the stroma of chloroplasts.
Light dependent reaction occurs in grana of chloroplast.
Ribosomes are necessary for protein synthesis.
Chlorophyll is green photosynthetic pigment found in chloroplasts.
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This is relatively inactive and highly soluble sugar playing little direct role in
metabolism and so, making an ideal transport sugar.
20.(a) The oxalic acid is reduced to malic acid in mesophyll cells, from
chloroplast of mesophyll cells the malic acid is transferred to the chloroplast
of bundle sheath cells where, it is decarboxylated to form CO2 and pyruvic
acid.
P700 A1 A2 A3
Chl-a+ phyloquinone Fe-S PRotein P430
The reduced P430 passes its electrons to ferredoxin (Fd) present at outer surface
of thylakoid membrane.
24.(c) In C4 -plants every CO2 molecule has to be fixed twice, so these plants are
needed more energy for the synthesis of hexose sugar molecules than C3-
plants in which CO2 has to be fixed only once. 18 ATP molecules are required
by C3 -plants for the synthesis of one molecule of hexose sugar while 30 ATP
molecules are needed by the C4 -plants for the same. Thus, C4 -plants have a
need of 12 ATP molecules extra than C3 -plants for the synthesis of one
molecule of hexose sugar.
25. (d) The fixation of CO2 in C4 -plants takes place in two places and by two
different organic compounds, Phosphoenol Pyruvate (PEP) is found in mesophyll
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cells which primarily fixes atmospheric CO2 into oxalo acetic acid(4C)RuBisCO) is
present in bundle sheath cells where final fixation of CO2 in hexose sugars takes
place, CO2 is primarily fixed by PEP carboxylase because this enzyme has greater
affinity to CO2 than RuBisCO.
26.(c) In C3 -plants the first stable product formed during dark reaction is 3-
phosphoglycerie acid. Since, it is a 3-carbon compound hence, the pathway is
referred as C3 -pathway. Oxalo Acetic Acid (OAA) is the first stable compound
in C4-plants. It is a 4C compound,
27.(a) Shade tolerant plants have lower photosynthetic rates and hence, lower
growth rates. On the other hand, these plants have larger photosynthetic unit
size than the sun plants.
29.(a) Carotenoids are a group of yellow, red and orange pigments which function
as accessory pigments and protect chlorophyll molecules from destruction by
intensive light rays. Carotenoids have three absorption peaks in the blue-violet
range of the spectrum.
Malic Acid
+
NADP+
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34.(b) As a result of light reaction, oxygen, NADPH and ATP are formed, Oxygen is
released into the atmosphere while NADPH and ATP are utilised for reduction
of CO2 to carbohydrate in dark reaction.
35.(c) The fall in photosynthetic yield beyond red region of spectrum (680 nm) is
called red drop. Reaction centre of PS-II is P680 while that of PS-I is P700. So in
the red drop reaction PS-II is inactivated.
36. (a) Maize is a C4 -plant. C4-plants have Kranz type anatomy of leaves.
PGA (3-Phosphoglyceric Acid) is formed during Calvin cycle.
OAA (Oxalo Acetic Acid) a 4C compound is formed during Hatch and Slack cycle
(C4 cycle).
37. (c) Conversion of CO2 to simple (reduced) organic compounds is called CO2
assimilation or CO2 fixation or carbon fixation. This fixation pathway was
elucidated in the early 1950s by Melvin Calvin and Coworkers and is often called
as Calvin cycle.
Since, one molecule of carbon is fixed in one turn of the Calvin cycle. So, SIX turns
of the cycle are required to fix the glucose molecule containing 6 carbon atoms.
38. (a) The entire process of photosynthesis is driven by light energy coming
from the sun. This energy is first captured by chlorophyll molecules and later on
utilised for the synthesis of ATP (chemical energy) molecules which are later
utilised in the dark reaction, i.e., Calvin cycle.
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40. (d) CO2 is fixed in the stroma of the chloroplast leading to the synthesis of
PGA from which glyceraldehyde phosphate is formed. From gayceraldehyde
phosphate, sugar and starch are formed. All these do not require light. However,
photolysis of water and phosphorylation of ADP to ATP requires light energy.
41. (b) RuBisCO (RuBP carboxylase) is the most abundant protein on this
planet, RuBisCO constitutes 16% of chloroplast protein, It is required for CO2
fixation with RuBP (Ribulose Biphosphate) in Calvin cycle.
44. (a) The principle of limiting factors was given by Blackmann, a British plant
physiologist in 1905, according to him, light intensity, carbon dioxide
concentration and temperature are the limiting factors in photosynthesis. When a
process is conditioned as to its rapidity by a number of separate factors, the rate
of the process is limited by the pace of the slowest factor.
45. (a) In dark phase or Calvin cycle, carbon dioxide is assimilated with the help
of assimilatory power (ATP and NADPH2) to produce organic acid, The cycle
involves reduction of carbon involving carboxylation, glycolytic reversal and
regeneration of RuBP. C3 cycle is also known as reductive pentose pathway or
Photosynthetic Carbon Reduction (PCR).
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47. (a) Chlorophyll has a tetrapyrrole porphyrin head and a long chain alcohol
called phytol tail. Each pyrrole is a 5 member ring with one nitrogen and four
carbon. A non-ionic Mg atom lies in the centre of porphyrin, attached to nitrogen
atoms of pyrrole rings Chloroohyll-a has methyl group at carbon 3 of pyrrole ring
and chlorophyll-b has formyl (aldehyde) group attached to this atom.
49. (c) Photosynthetic pigment molecules (e.g. P700, P680) are able to convert
light energy into chemical energy. These pigment molecules which together
forms the photosynthetic units, possess photocentres (reaction centre = trap
centre) surrounded by harvesting molecules differentiated into core molecules
and antenna molecules.
52. (b) 90% of total photosynthesis is carried out by aquatic plants, chiefly
algae (80% in oceans and 10% in freshwater), 10% of total photosynthesis is
performed by land plants.
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53. (b) Ruben, Hassid and Kamen (1941) using heavy isotope of oxygen, O18 in
water, found that oxygen evolved in photosynthesis comes from water. Evolution
of oxygen does not require carbon dioxide.
54. (d) Maximum solar energy is trapped by growing grasses, as they have the
largest surface area for absorption. Limited number of algal individual are
growing in tank so, they absorb limited amount of light.
56. (c) Cytochromes (Kelvin; 1925) are the electron transport intermediates
containing heme (or related prosthetic groups) in which the iron undergoes
valency changes during electron transfer and produces energy (ATP) in both
photosynthesis and respiration.
57. (d) In plants, translocation, i.e the movement of organic nutrients from the
region of supply to the region of sink or utilisation occurs through phloem (sieve
tube/sieve cells) tissue. Translocated organic nutrients constitute 10-26%
carbohydrates (usually sucrose) and 1% nitrogenous components (mostly amino
acids).
58. (c) PS-II is present in appressed part of granal thylakoids. PS-I is present in
stroma thylakoids and non-appressed parts of granal thylakoids.
59. (d) The plants in which the first stable product of dark reaction of
photosynthesis is a 4-carbon compound called C4 -plants, e g. sugarcane, maize,
sorghum, These plants shows characteristic Kranz anatomy. The first CO2
acceptor in these plants is Phosphopheoenol Pyruvate (PEP).
60. (b) In C4-plants, mesophyll cells fix carbon dioxide with the help of
phosphophoenol-pyruvate (the first acceptor) in the presence of PEP carboxylase
compound oxaloacetic acid (first product).
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62. (C) Light reaction of photosynthesis occurs 'in granal thylakoid membranes
of chloroplast while dark reaction occurs in the stroma or matrix, i.e. outside the
photosynthetic lamellae of chloroplast.
64. (a) Ferredoxin (Fd) is a soluble protein which acts as electron carrier and
forms a constituent of PS-I Ferredoxin passes electrons to reductase complex
which helps in reducing NADP+ to NADPH (a strong reducing agent).
65. (b) According to the principle of limiting factor, the rate of the process is
limited by the pace of the slowest factor. Light intensity varies with latitude,
altitude, season, topography, presence or absence of interceptors like cloud, dust,
fog, humidity etc. In Eastern states, low light intensity during monsoon results in
low photosynthesis and hence, lesser yield.
66. (c) Calvin, Benson and Basshan utilized C14 (with long life) to trace the path
of carbon in photosynthesis. Calvin was awarded Nobel Prize in 1961 in
recognition to his work with CI4 isotope. He discovered the cycle involved in
carbon assimilation, known as Calvin cycle or C3-cycle.
67. (a) Leaves of C4 -plants (e.g. sugarcane, maize) are characterized by Kranz
anatomy in which the mesophyll is undifferentiated and its cells occur in
concentric layers around vascular bundles. Vascular bundles are surrounded by
large sized bundle sheath cells which are arranged in a wreath like manner (Kranz-
wreath).
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69. (a) C4 plants possess two types of chloroplasts granal in mesophyll cells and
agranal in bundle sheath cells. Mesophyll cells are specialized to perform light
reaction and bundle sheath cells possess RuBisCO, here CO2 is fixed through
Calvin cycle.
72. (c) A chlorophyll molecule consists of two parts, the porphyrin ring (head)
15 x 15 Å and a phytol tail (20 Å)
73. (d) In dark reaction of reducing agent (NADPH) and source of energy (ATP)
formed during light reaction, are utilised in the conversion of CO2 to
carbohydrates.
NADP Reduces
NADP+ + 2e- + H+ NADPH
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• 41 YEARS OF JEE MPC PAPERS (CHAPTER WISE, TOPIC WISE WITH SOLUTIONS)
• 32 YEARS OF NEET BPC PAPERS (CHAPTER WISE, TOPIC WISE WITH SOLUTIONS)
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